The Journal of Cell Biology, Vol 110, 803-815, Copyright © 1990 by The Rockefeller University Press

ARTICLES

Novel function for beta 1 integrins in keratinocyte cell-cell interactions

H Larjava, J Peltonen, SK Akiyama, SS Yamada, HR Gralnick, J Uitto and KM Yamada
Membrane Biochemistry Section, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892.

We have examined the expression, localization, and function of beta 1 integrins on cultured human epidermal keratinocytes using polyclonal and monoclonal antibodies against the beta 1, alpha 2, alpha 3, and alpha 5 integrin subunits. The beta 1 polypeptide, common to all class 1 integrins, was localized primarily in areas of cell-cell contacts of cultured keratinocytes, as were alpha 2 and alpha 3 polypeptides, suggesting a possible role in cell-cell adhesion for these integrin polypeptides. In contrast, the fibronectin receptor alpha 5 subunit showed no such accumulations in regions of cell-cell contact but was more diffusely distributed in the keratinocyte plasma membrane, consistent with the absence of fibronectin at cell-cell contact sites. Colonies of cultured keratinocytes could be dissociated by treatment with monoclonal antibody specific to the beta 1 polypeptide. Such dissociation of cell-cell contacts also occurred under conditions where the monoclonal antibody had no effect on cell-substrate adhesion. Therefore, beta 1 integrin-dependent cell-cell adhesion can be inhibited without affecting other cell-adhesive interactions. Antibody treatment of keratinocytes maintained in either low (0.15 mM) or high (1.2 mM) CaCl2 also resulted in the loss of organization of intracellular F-actin filaments and beta 1 integrins, even when the anti-beta 1 monoclonal antibody had no dissociating effect on keratinocyte colonies at the higher calcium concentration. Our results indicate that beta 1 integrins play roles in the maintenance of cell- cell contacts between keratinocytes and in the organization of intracellular microfilaments. They suggest that in epithelial cells integrins can function in cell-cell interactions as well as in cell- substrate adhesion.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Facebook    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

• Velozo, J, Aguilera, S, Alliende, C, Ewert, P, Molina, C, Perez, P, Leyton, L, Quest, A, Brito, M, Gonzalez, S, Leyton, C, Hermoso, M, Romo, R, Gonzalez, M-J (2009). Severe alterations in expression and localisation of {alpha}6{beta}4 integrin in salivary gland acini from patients with Sjogren syndrome. Ann Rheum Dis 68: 991-996 [Abstract] [Full Text]  
• Yu, W., Fang, X., Ewald, A., Wong, K., Hunt, C. A., Werb, Z., Matthay, M. A., Mostov, K. (2007). Formation of Cysts by Alveolar Type II Cells in Three-dimensional Culture Reveals a Novel Mechanism for Epithelial Morphogenesis. Mol. Biol. Cell 18: 1693-1700 [Abstract] [Full Text]  
• Praetorius, H. A., Praetorius, J., Nielsen, S., Frokiaer, J., Spring, K. R. (2004). {beta}1-Integrins in the primary cilium of MDCK cells potentiate fibronectin-induced Ca2+ signaling. Am. J. Physiol. Renal Physiol. 287: F969-F978 [Abstract] [Full Text]  
• Siu, M. K.Y., Cheng, C. Y. (2004). Extracellular Matrix: Recent Advances on Its Role in Junction Dynamics in the Seminiferous Epithelium During Spermatogenesis. Biol. Reprod. 71: 375-391 [Abstract] [Full Text]  
• Nymalm, Y., Puranen, J. S., Nyholm, T. K. M., Kapyla, J., Kidron, H., Pentikainen, O. T., Airenne, T. T., Heino, J., Slotte, J. P., Johnson, M. S., Salminen, T. A. (2004). Jararhagin-derived RKKH Peptides Induce Structural Changes in {alpha}1I Domain of Human Integrin {alpha}1{beta}1. J. Biol. Chem. 279: 7962-7970 [Abstract] [Full Text]  
• Chattopadhyay, N., Wang, Z., Ashman, L. K., Brady-Kalnay, S. M., Kreidberg, J. A. (2003). {alpha}3{beta}1 integrin-CD151, a component of the cadherin-catenin complex, regulates PTP{micro} expression and cell-cell adhesion. JCB 163: 1351-1362 [Abstract] [Full Text]  
• Koivisto, L., Alavian, K., Hakkinen, L., Pelech, S., McCulloch, C. A., Larjava, H. (2003). Glycogen synthase kinase-3 regulates formation of long lamellipodia in human keratinocytes. J. Cell Sci. 116: 3749-3760 [Abstract] [Full Text]  
• Doornaert, B., Leblond, V., Galiacy, S., Gras, G., Planus, E., Laurent, V., Isabey, D., Lafuma, C. (2003). Negative impact of DEP exposure on human airway epithelial cell adhesion, stiffness, and repair. Am. J. Physiol. Lung Cell. Mol. Physiol. 284: L119-L132 [Abstract] [Full Text]  
• Schreider, C., Peignon, G., Thenet, S., Chambaz, J., Pincon-Raymond, M. (2002). Integrin-mediated functional polarization of Caco-2 cells through E-cadherin--actin complexes. J. Cell Sci. 115: 543-552 [Abstract] [Full Text]  
• Yanez-Mo, M, Tejedor, R, Rousselle, P, Sanchez -Madrid, F (2001). Tetraspanins in intercellular adhesion of polarized epithelial cells: spatial and functional relationship to integrins and cadherins. J. Cell Sci. 114: 577-587 [Abstract]  
• Bergin, E., Levine, J. S., Koh, J. S., Lieberthal, W. (2000). Mouse proximal tubular cell-cell adhesion inhibits apoptosis by a cadherin-dependent mechanism. Am. J. Physiol. Renal Physiol. 278: F758-F768 [Abstract] [Full Text]  
• Katz, J., Sambandam, V., Wu, J. H., Michalek, S. M., Balkovetz, D. F. (2000). Characterization of Porphyromonas gingivalis-Induced Degradation of Epithelial Cell Junctional Complexes. Infect. Immun. 68: 1441-1449 [Abstract] [Full Text]  
• Yu, X, Miyamoto, S, Mekada, E (2000). Integrin alpha 2 beta 1-dependent EGF receptor activation at cell-cell contact sites. J. Cell Sci. 113: 2139-2147 [Abstract]  
• Wang, Z, Symons, J., Goldstein, S., McDonald, A, Miner, J., Kreidberg, J. (1999). (Alpha)3(beta)1 integrin regulates epithelial cytoskeletal organization. J. Cell Sci. 112: 2925-2935 [Abstract]  
• Zuk, A., Bonventre, J. V., Brown, D., Matlin, K. S. (1998). Polarity, integrin, and extracellular matrix dynamics in the postischemic rat kidney. Am. J. Physiol. Cell Physiol. 275: C711-C731 [Abstract] [Full Text]  
• Yanez-Mo, M., Alfranca, A., Cabanas, C., Marazuela, M., Tejedor, R., Angeles Ursa, M., Ashman, L. K., de Landazuri, M. O., Sanchez-Madrid, F. (1998). Regulation of Endothelial Cell Motility by Complexes of Tetraspan Molecules CD81/TAPA-1 and CD151/PETA-3 with {alpha}3{beta}1 Integrin Localized at Endothelial Lateral Junctions. JCB 141: 791-804 [Abstract] [Full Text]  
• Aldo Del Maschio, , Martin-Padura, I., Bernasconi, S., Dejana, E. (1997). Triggering of ß1-Integrin Chain Induces Platelet Adhesion to Cultured Endothelium. Arterioscler. Thromb. Vasc. Bio. 17: 2663-2671 [Abstract] [Full Text]  
• Stepp, M. A., Zhu, L. (1997). Upregulation of {alpha}9 Integrin and Tenascin During Epithelial Regeneration After Debridement in the Cornea. J. Histochem. Cytochem. 45: 189-202 [Abstract] [Full Text]  
• Desban, N, Duband, J. (1997). Avian neural crest cell migration on laminin: interaction of the alpha1beta1 integrin with distinct laminin-1 domains mediates different adhesive responses. J. Cell Sci. 110: 2729-2744 [Abstract]  
• Christopher, R., Kowalczyk, A., McKeown-Longo, P. (1997). Localization of fibronectin matrix assembly sites on fibroblasts and endothelial cells. J. Cell Sci. 110: 569-581 [Abstract]  
• Kitajima, I., Kawahara, K., Hanyu, N., Shin, H., Tokioka, T., Soejima, Y., Tsutsui, J., Ozawa, M., Shimayama, T., Maruyama, I. (1996). Enhanced E-cadherin expression and increased calcium-dependent cell-cell adhesion in human T-cell leukemia virus type I Tax-expressing PC12 cells. J. Cell Sci. 109: 609-617 [Abstract]  
• Zuk, A, Matlin, K. (1996). Apical beta 1 integrin in polarized MDCK cells mediates tubulocyst formation in response to type I collagen overlay. J. Cell Sci. 109: 1875-1889 [Abstract]  
• Weitzman, J., Chen, A, Hemler, M. (1995). Investigation of the role of beta 1 integrins in cell-cell adhesion. J. Cell Sci. 108: 3635-3644 [Abstract]  
• Symington, B., Carter, W. (1995). Modulation of epidermal differentiation by epiligrin and integrin alpha 3 beta 1. J. Cell Sci. 108: 831-838 [Abstract]  
• Berdichevsky, F, Alford, D, D'Souza, B, Taylor-Papadimitriou, J (1994). Branching morphogenesis of human mammary epithelial cells in collagen gels. J. Cell Sci. 107: 3557-3568 [Abstract]  
• Milstone, L., Hough-Monroe, L, Kugelman, L., Bender, J., Haggerty, J. (1994). Epican, a heparan/chondroitin sulfate proteoglycan form of CD44, mediates cell-cell adhesion. J. Cell Sci. 107: 3183-3190 [Abstract]  
• Rabino, M, Trusolino, L, Prat, M, Cremona, O, Savoia, P, Marchisio, P. (1994). A monoclonal antibody identifies a novel GPI-anchored glycoprotein involved in epithelial intercellular adhesion. J. Cell Sci. 107: 1413-1428 [Abstract]  
• Ojakian, G., Schwimmer, R (1994). Regulation of epithelial cell surface polarity reversal by beta 1 integrins. J. Cell Sci. 107: 561-576 [Abstract]  
• Schoenenberger, C., Zuk, A, Zinkl, G., Kendall, D, Matlin, K. (1994). Integrin expression and localization in normal MDCK cells and transformed MDCK cells lacking apical polarity. J. Cell Sci. 107: 527-541 [Abstract]  
• Watt, F., Kubler, M., Hotchin, N., Nicholson, L., Adams, J. (1993). Regulation of keratinocyte terminal differentiation by integrin-extracellular matrix interactions. J. Cell Sci. 106: 175-182 [Abstract]  
• Zambruno, G, Marchisio, P., Melchiori, A, Bondanza, S, Cancedda, R, De Luca, M (1993). Expression of integrin receptors and their role in adhesion, spreading and migration of normal human melanocytes. J. Cell Sci. 105: 179-190 [Abstract]  
• Tarone, G, Russo, M., Hirsch, E, Odorisio, T, Altruda, F, Silengo, L, Siracusa, G (1993). Expression of beta 1 integrin complexes on the surface of unfertilized mouse oocyte. Development 117: 1369-1375 [Abstract]  
• Geiger, B., Salomon, D., Takeichi, M., Hynes, R. O. (1992). A chimeric N-cadherin/beta 1-integrin receptor which localizes to both cell-cell and cell-matrix adhesions. J. Cell Sci. 103: 943-951 [Abstract]  
• Kim, L. T., Ishihara, S., Lee, C. C., Akiyama, S. K., Yamada, K. M., Grinnell, F. (1992). Altered glycosylation and cell surface expression of beta 1 integrin receptors during keratinocyte activation. J. Cell Sci. 103: 743-753 [Abstract]  
• Uitto, V.-J., Larjava, H., Peltonen, J., Brunette, D.M. (1992). Expression of Fibronectin and Integrins in Cultured Periodontal Ligament Epithelial Cells. JDR 71: 1203-1211 [Abstract]  
• Duband, J., Belkin, A., Syfrig, J, Thiery, J., Koteliansky, V. (1992). Expression of alpha 1 integrin, a laminin-collagen receptor, during myogenesis and neurogenesis in the avian embryo. Development 116: 585-600 [Abstract]  
• Chan, B., Matsuura, N, Takada, Y, Zetter, B., Hemler, M. (1991). In vitro and in vivo consequences of VLA-2 expression on rhabdomyosarcoma cells. Science 251: 1600-1602 [Abstract]  
• Takeichi, M (1991). Cadherin cell adhesion receptors as a morphogenetic regulator. Science 251: 1451-1455 [Abstract]  
• Uitto, V.-J., Larjava, H. (1991). Extracellular Matrix Molecules and their Receptors: An Overview with Special Emphasis on Periodontal Tissues. CROBM 2: 323-354 [Abstract] [Full Text]  
• Hormia, M., Ylanne, J., Virtanen, I. (1990). Expression of Integrins in Human Gingiva. JDR 69: 1817-1823 [Abstract]  

Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents