cDNAs of cell adhesion molecules of different specificity induce changes in cell shape and border formation in cultured S180 cells

doi:10.1083/jcb.110.4.1239

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cDNAs of cell adhesion molecules of different specificity induce changes in cell shape and border formation in cultured S180 cells

F Matsuzaki, RM Mege, SH Jaffe, DR Friedlander, WJ Gallin, JI Goldberg, BA Cunningham and GM Edelman
Rockefeller University, New York 10021.

The liver cell adhesion molecule (L-CAM) and N-cadherin or adherens junction-specific CAM (A-CAM) are structurally related cell surface glycoproteins that mediate calcium-dependent adhesion in different tissues. We have isolated and characterized a full-length cDNA clone for chicken N-cadherin and used this clone to transfect S180 mouse sarcoma cells that do not normally express N-cadherin. The transfected cells (S180cadN cells) expressed N-cadherin on their surfaces and resembled S180 cells transfected with L-CAM (S180L cells) in that at confluence they formed an epithelioid sheet and displayed a large increase in the number of adherens and gap junctions. In addition, N- cadherin in S180cadN cells, like L-CAM in S180L cells, accumulated at cellular boundaries where it was colocalized with cortical actin. In S180L cells and S180cadN cells, L-CAM and N-cadherin were seen at sites of adherens junctions but were not restricted to these areas. Adhesion mediated by either CAM was inhibited by treatment with cytochalasin D that disrupted the actin network of the transfected cells. Despite their known structural similarities, there was no evidence of interaction between L-CAM and N-cadherin. Doubly transfected cells (S180L/cadN) also formed epithelioid sheets. In these cells, both N- cadherin and L-CAM colocalized at areas of cell contact and the presence of antibodies to both CAMs was required to disrupt the sheets of cells. Studies using divalent antibodies to localize each CAM at the cell surface or to perturb their distributions indicated that in the same cell there were no interactions between L-CAM and N-cadherin molecules. These data suggest that the Ca(++)-dependent CAMs are likely to play a critical role in the maintenance of epithelial structures and support a model for the segregation of CAM mediated binding. They also provide further support for the so-called precedence hypothesis that proposes that expression and homophilic binding of CAMs are necessary for formation of junctional structures in epithelia.
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Gavard, J., Lambert, M., Grosheva, I., Marthiens, V., Irinopoulou, T., Riou, J.-F., Bershadsky, A., Mege, R.-M. (2004). Lamellipodium extension and cadherin adhesion: two cell responses to cadherin activation relying on distinct signalling pathways. J. Cell Sci. 117: 257-270 [Abstract] [Full Text]
Renaud-Young, M., Gallin, W. J. (2002). In the First Extracellular Domain of E-cadherin, Heterophilic Interactions, but Not the Conserved His-Ala-Val Motif, Are Required for Adhesion. J. Biol. Chem. 277: 39609-39616 [Abstract] [Full Text]
Lin, J. H.-C., Takano, T., Cotrina, M. L., Arcuino, G., Kang, J., Liu, S., Gao, Q., Jiang, L., Li, F., Lichtenberg-Frate, H., Haubrich, S., Willecke, K., Goldman, S. A., Nedergaard, M. (2002). Connexin 43 Enhances the Adhesivity and Mediates the Invasion of Malignant Glioma Cells. J. Neurosci. 22: 4302-4311 [Abstract] [Full Text]
Schreider, C., Peignon, G., Thenet, S., Chambaz, J., Pincon-Raymond, M. (2002). Integrin-mediated functional polarization of Caco-2 cells through E-cadherin--actin complexes. J. Cell Sci. 115: 543-552 [Abstract] [Full Text]
Naka, T, Oda, Y, Iwamoto, Y, Shinohara, N, Chuman, H, Fukui, M, Tsuneyoshi, M (2001). Immunohistochemical analysis of E-cadherin, {alpha}-catenin, {beta}-catenin, {gamma}-catenin, and neural cell adhesion molecule (NCAM) in chordoma. J. Clin. Pathol. 54: 945-950 [Abstract] [Full Text]
Xu, X., Li, W.E.I., Huang, G.Y., Meyer, R., Chen, T., Luo, Y., Thomas, M.P., Radice, G.L., Lo, C.W. (2001). Modulation of mouse neural crest cell motility by N-cadherin and connexin 43 gap junctions. JCB 154: 217-230 [Abstract] [Full Text]
Zhong, Y., Delgado, Y., Gomez, J., Lee, S. W., Perez-Soler, R. (2001). Loss of H-Cadherin Protein Expression in Human Non-Small Cell Lung Cancer Is Associated with Tumorigenicity. Clin. Cancer Res. 7: 1683-1687 [Abstract] [Full Text]
Lambert, M, Padilla, F, Mege, R. (2000). Immobilized dimers of N-cadherin-Fc chimera mimic cadherin-mediated cell contact formation: contribution of both outside-in and inside-out signals. J. Cell Sci. 113: 2207-2219 [Abstract]
Hsu, M, Andl, T, Li, G, Meinkoth, J., Herlyn, M (2000). Cadherin repertoire determines partner-specific gap junctional communication during melanoma progression. J. Cell Sci. 113: 1535-1542 [Abstract]
Nieman, M. T., Prudoff, R. S., Johnson, K. R., Wheelock, M. J. (1999). N-Cadherin Promotes Motility in Human Breast Cancer Cells Regardless of Their E-Cadherin Expression. JCB 147: 631-644 [Abstract] [Full Text]
Kostin, S., Hein, S., Bauer, E. P., Schaper, J. (1999). Spatiotemporal Development and Distribution of Intercellular Junctions in Adult Rat Cardiomyocytes in Culture. Circ. Res. 85: 154-167 [Abstract] [Full Text]
Iwata, F., Joh, T., Ueda, F., Yokoyama, Y., Itoh, M. (1998). Role of gap junctions in inhibiting ischemia-reperfusion injury of rat gastric mucosa. Am. J. Physiol. Gastrointest. Liver Physiol. 275: G883-G888 [Abstract] [Full Text]
Araki, T., Zimonjic, D. B., Popescu, N. C., Milbrandt, J. (1997). Mechanism of Homophilic Binding Mediated by Ninjurin, a Novel Widely Expressed Adhesion Molecule. J. Biol. Chem. 272: 21373-21380 [Abstract] [Full Text]
Edlund, M., Blikstad, I., Obrink, B.�r. (1996). Calmodulin Binds to Specific Sequences in the Cytoplasmic Domain of C-CAM and Down-regulates C-CAM Self-association. J. Biol. Chem. 271: 1393-1399 [Abstract] [Full Text]
Hunter, I, Lindh, M, Obrink, B (1994). Differential regulation of C-CAM isoforms in epithelial cells. J. Cell Sci. 107: 1205-1216 [Abstract]
Cifuentes-Diaz, C, Nicolet, M, Goudou, D, Rieger, F, Mege, R. (1994). N-cadherin expression in developing, adult and denervated chicken neuromuscular system: accumulations at both the neuromuscular junction and the node of Ranvier. Development 120: 1-11 [Abstract]
Mege, R. M., Goudou, D., Diaz, C., Nicolet, M., Garcia, L., Geraud, G., Rieger, F. (1992). N-cadherin and N-CAM in myoblast fusion: compared localisation and effect of blockade by peptides and antibodies. J. Cell Sci. 103: 897-906 [Abstract]
Kintner, C. (1992). Cadherins and the Morphogenesis of Epithelial Tissues in Xenopus Embryos. Cold Spring Harb Symp Quant Biol 57: 335-344 [Abstract]
Franke, W.W., Troyanovsky, S.M., Koch, P.J., Troyanovsky, R., Fouquet, B., Leube, R.E. (1992). Desmosomal Proteins: Mediators of Intercellular Coupling and Intermediate Filament Anchorage. Cold Spring Harb Symp Quant Biol 57: 643-652 [Abstract]
Takeichi, M (1991). Cadherin cell adhesion receptors as a morphogenetic regulator. Science 251: 1451-1455 [Abstract]
Edelman, G.M., Cunningham, B.A. (1990). Place-dependent Cell Adhesion, Process Retraction, and Spatial Signaling in Neural Morphogenesis. Cold Spring Harb Symp Quant Biol 55: 303-318 [Abstract]
Mary, S., Charrasse, S., Meriane, M., Comunale, F., Travo, P., Blangy, A., Gauthier-Rouviere, C. (2002). Biogenesis of N-Cadherin-dependent Cell-Cell Contacts in Living Fibroblasts Is a Microtubule-dependent Kinesin-driven Mechanism. Mol. Biol. Cell 13: 285-301 [Abstract] [Full Text]