Purified N-cadherin is a potent substrate for the rapid induction of neurite outgrowth

doi:10.1083/jcb.110.4.1253

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Purified N-cadherin is a potent substrate for the rapid induction of neurite outgrowth

JL Bixby and R Zhang
Department of Pharmacology, University of Miami School of Medicine, Florida 33136.

N-cadherin is the predominant mediator of calcium-dependent adhesion in the nervous system (Takeichi, M. 1988. Development (Camb.). 102: 639- 655). Investigations using antibodies to block N-cadherin function (Bixby, J.L., R.L. Pratt, J. Lilien, and L.F. Reichardt. 1987. Proc. Natl. Acad. Sci. USA. 84:2555-2569; Bixby, J.L., J. Lilien, and L.F. Reichardt. 1988. J. Cell Biol. 107:353-362; Tomaselli, K.J., K.N. Neugebauer, J.L. Bixby, J. Lilien, and L.F. Reichardt. 1988. Neuron. 1:33-43) or transfection of the N-cadherin gene into heterologous cell lines (Matsunaga, M., K. Hatta, A. Nagafuchi, and M. Takeichi. 1988. Nature (Lond.). 334:62-64) have provided evidence that N-cadherin, alone or in combination with other molecules, can participate in the induction of neurite extension. We have developed an affinity purification procedure for the isolation of whole N-cadherin from chick brain and have used the isolated protein as a substrate for neurite outgrowth. N-cadherin promotes the rapid extension of neurites from chick ciliary ganglion neurons, which extend few or no neurites on adhesive but noninducing substrates such as polylysine, tissue culture plastic, and collagens. N-cadherin is extremely potent, more so than the L1 adhesion molecule, and comparable to the extracellular matrix protein laminin. Compared to laminin, however. N-cadherin promotes outgrowth from ciliary ganglion neurons extremely rapidly and with a distinct morphology. These results provide a direct demonstration that N-cadherin is sufficient to induce neurite outgrowth when substrate bound and suggest that the mechanism(s) involved may differ from that induced by laminin.
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Balsamo, J., Arregui, C., Leung, T., Lilien, J. (1998). The Nonreceptor Protein Tyrosine Phosphatase PTP1B Binds to the Cytoplasmic Domain of N-Cadherin and Regulates the Cadherin-Actin Linkage. JCB 143: 523-532 [Abstract] [Full Text]
Ozawa, M., Kemler, R. (1998). The Membrane-proximal Region of the E-Cadherin Cytoplasmic Domain Prevents Dimerization and Negatively Regulates Adhesion Activity. JCB 142: 1605-1613 [Abstract] [Full Text]
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Shibata, T., Shimoyama, Y., Gotoh, M., Hirohashi, S. (1997). Identification of Human Cadherin-14, a Novel Neurally Specific Type II Cadherin, by Protein Interaction Cloning. J. Biol. Chem. 272: 5236-5240 [Abstract] [Full Text]
Fredette, B., Miller, J, Ranscht, B (1996). Inhibition of motor axon growth by T-cadherin substrata. Development 122: 3163-3171 [Abstract]
Clarke, G., Moss, D. (1994). Identification of a novel protein from adult chicken brain that inhibits neurite outgrowth. J. Cell Sci. 107: 3393-3402 [Abstract]
Levine, E, Lee, C., Kintner, C, Gumbiner, B. (1994). Selective disruption of E-cadherin function in early Xenopus embryos by a dominant negative mutant. Development 120: 901-909 [Abstract]
Takeichi, M (1991). Cadherin cell adhesion receptors as a morphogenetic regulator. Science 251: 1451-1455 [Abstract]
Takeichi, M., Inuzuka, H., Shimamura, K., Fujimori, T., Nagafuchi, A. (1990). Cadherin Subclasses: Differential Expression and Their Roles in Neural Morphogenesis. Cold Spring Harb Symp Quant Biol 55: 319-325 [Abstract]