Fatty acylation promotes fusion of transport vesicles with Golgi cisternae

doi:10.1083/jcb.110.4.955

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Fatty acylation promotes fusion of transport vesicles with Golgi cisternae

N Pfanner, BS Glick, SR Arden and JE Rothman
Department of Biology, Princeton University, New Jersey 08544-1014.

Two different methods, stimulation of transport by fatty acyl-coenzyme A (CoA) and inhibition of transport by a nonhydrolyzable analogue of palmitoyl-CoA, reveal that fatty acylation is required to promote fusion of transport vesicles with Golgi cisternae. Specifically, fatty acyl-CoA is needed after the attachment of coated vesicles and subsequent uncoating of the vesicles, and after the binding of the NEM- sensitive fusion protein (NSF) to the membranes, but before the actual fusion event. We therefore suggest that an acylated transport component participates, directly or indirectly, in membrane fusion.
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