Mapping enteroendocrine cell populations in transgenic mice reveals an unexpected degree of complexity in cellular differentiation within the gastrointestinal tract

doi:10.1083/jcb.110.5.1791

This Article

Full Text (PDF, 9705K)

Alert me when this article is cited

Citation Map

Services

Email this article

Similar articles in this journal

Similar articles in PubMed

Alert me to new content in the JCB

Download to citation manager

Citing Articles

Citing Articles via HighWire

Citing Articles via CrossRef

Citing Articles via Google Scholar

Google Scholar

Articles by Roth, K. A.

Articles by Gordon, J. I.

Search for Related Content

PubMed

PubMed Citation

Articles by Roth, K. A.

Articles by Gordon, J. I.

Pubmed/NCBI databases

Compound via MeSH
Substance via MeSH

Social Bookmarking

What's this?

ARTICLES

Mapping enteroendocrine cell populations in transgenic mice reveals an unexpected degree of complexity in cellular differentiation within the gastrointestinal tract

KA Roth, JM Hertz and JI Gordon
Department of Pathology, Washington University School of Medicine, St. Louis, Missouri 63110.

The gastrointestinal tract is lined with a monolayer of cells that undergo perpetual and rapid renewal. Four principal, terminally differentiated cell types populate the monolayer, enterocytes, goblet cells, Paneth cells, and enteroendocrine cells. This epithelium exhibits complex patterns of regional differentiation, both from crypt- to-villus and from duodenum-to-colon. The "liver" fatty acid binding protein (L-FABP) gene represents a useful model for analyzing the molecular basis for intestinal epithelial differentiation since it exhibits cell-specific, region-specific, as well as developmental stage specific expression. We have previously linked portions of the 5' nontranscribed domain of the rat L-FABP gene to the human growth hormone (hGH) gene and analyzed expression of the fusion gene in adult transgenic mice. High levels of hGH expression were noted in enterocytes as well as cells that histologically resembled enteroendocrine cells. In the present study, we have used immunocytochemical techniques to map the distribution of enteroendocrine cells in the normal adult mouse gut and to characterize those that synthesize L-FABP. In addition, L-FABP/hGH fusion genes were used to identify subsets of enteroendocrine cells based on their ability to support hGH synthesis in several different pedigrees of transgenic mice. The results reveal remarkable differences in transgene expression between, and within, enteroendocrine cell populations previously classified only on the basis of their neuroendocrine products. In some cases, these differences are related to the position occupied by cells along the duodenal-to-colonic and crypt-to-villus axes of the gut. Thus, transgenes appear to be sensitive tools for examining the cellular and regional differentiation of this class of intestinal epithelial cells.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Samuel, B. S., Shaito, A., Motoike, T., Rey, F. E., Backhed, F., Manchester, J. K., Hammer, R. E., Williams, S. C., Crowley, J., Yanagisawa, M., Gordon, J. I. (2008). Effects of the gut microbiota on host adiposity are modulated by the short-chain fatty-acid binding G protein-coupled receptor, Gpr41. Proc. Natl. Acad. Sci. USA 105: 16767-16772 [Abstract] [Full Text]
Bogunovic, M., Dave, S. H., Tilstra, J. S., Chang, D. T. W., Harpaz, N., Xiong, H., Mayer, L. F., Plevy, S. E. (2007). Enteroendocrine cells express functional Toll-like receptors. Am. J. Physiol. Gastrointest. Liver Physiol. 292: G1770-G1783 [Abstract] [Full Text]
Markovics, J. A., Carroll, P. A., Robles, M. T. S., Pope, H., Coopersmith, C. M., Pipas, J. M. (2005). Intestinal Dysplasia Induced by Simian Virus 40 T Antigen Is Independent of p53. J. Virol. 79: 7492-7502 [Abstract] [Full Text]
Drori, S., Girnun, G. D., Tou, L., Szwaya, J. D., Mueller, E., Kia, X., Shivdasani, R. A., Spiegelman, B. M. (2005). Hic-5 regulates an epithelial program mediated by PPAR{gamma}. Genes Dev. 19: 362-375 [Abstract] [Full Text]
Schonhoff, S. E., Giel-Moloney, M., Leiter, A. B. (2004). Minireview: Development and Differentiation of Gut Endocrine Cells. Endocrinology 145: 2639-2644 [Abstract] [Full Text]
Boshuizen, J. A., Reimerink, J. H. J., Korteland-van Male, A. M., van Ham, V. J. J., Koopmans, M. P. G., Buller, H. A., Dekker, J., Einerhand, A. W. C. (2003). Changes in Small Intestinal Homeostasis, Morphology, and Gene Expression during Rotavirus Infection of Infant Mice. J. Virol. 77: 13005-13016 [Abstract] [Full Text]
Wang, S. Y., Chi, M. M.-Y., Li, L., Moley, K. H., Wice, B. M. (2003). Studies with GIP/Ins cells indicate secretion by gut K cells is KATP channel independent. Am. J. Physiol. Endocrinol. Metab. 284: E988-E1000 [Abstract] [Full Text]
Herrera, P. (2000). Adult insulin- and glucagon-producing cells differentiate from two independent cell lineages. Development 127: 2317-2322 [Abstract]
Strauss, B. L., Boime, I. (2000). Cellular Localization of the Human Chorionic Gonadotropin {beta}-Subunit in Transgenic Mouse Placenta. Endocrinology 141: 430-437 [Abstract] [Full Text]
Hill, M. E., Asa, S. L., Drucker, D. J. (1999). Essential Requirement for Pax6 in Control of Enteroendocrine Proglucagon Gene Transcription. Mol. Endocrinol. 13: 1474-1486 [Abstract] [Full Text]
Gum, J. R. Jr., Hicks, J. W., Gillespie, A.-M., Carlson, E. J., Komuves, L., Karnik, S., Hong, J. C., Epstein, C. J., Kim, Y. S. (1999). Goblet cell-specific expression mediated by the MUC2 mucin gene promoter in the intestine of transgenic mice. Am. J. Physiol. Gastrointest. Liver Physiol. 276: G666-G676 [Abstract] [Full Text]
Slorach, E., Campbell, F., Dorin, J. (1999). A mouse model of intestinal stem cell function and regeneration. J. Cell Sci. 112: 3029-3038 [Abstract]
Rindi, G, Ratineau, C, Ronco, A, Candusso, M., Tsai, M, Leiter, A. (1999). Targeted ablation of secretin-producing cells in transgenic mice reveals a common differentiation pathway with multiple enteroendocrine cell lineages in the small intestine. Development 126: 4149-4156 [Abstract]
Falk, P. G., Hooper, L. V., Midtvedt, T., Gordon, J. I. (1998). Creating and Maintaining the Gastrointestinal Ecosystem: What We Know and Need To Know from Gnotobiology. Microbiol. Mol. Biol. Rev. 62: 1157-1170 [Abstract] [Full Text]
Rokutan, K., Yamada, M., Torigoe, J., Saito, T. (1998). Transforming growth factor-beta inhibits proliferation and maturation of cultured guinea pig gastric pit cells. Am. J. Physiol. Gastrointest. Liver Physiol. 275: G526-G533 [Abstract] [Full Text]
Garabedian, E. M., Roberts, L. J.�J., McNevin, M. S., Gordon, J. I. (1997). Examining the Role of Paneth Cells in the Small Intestine by Lineage Ablation in Transgenic Mice. J. Biol. Chem. 272: 23729-23740 [Abstract] [Full Text]
Simon, T. C., Cho, A., Tso, P., Gordon, J. I. (1997). Suppressor and Activator Functions Mediated by a Repeated Heptad Sequence in the Liver Fatty Acid-binding Protein Gene (Fabpl). EFFECTS ON RENAL, SMALL INTESTINAL, AND COLONIC EPITHELIAL CELL GENE EXPRESSION IN TRANSGENIC MICE. J. Biol. Chem. 272: 10652-10663 [Abstract] [Full Text]
Hermiston, M L, Wong, M H, Gordon, J I (1996). Forced expression of E-cadherin in the mouse intestinal epithelium slows cell migration and provides evidence for nonautonomous regulation of cell fate in a self-renewing system.. Genes Dev. 10: 985-996 [Abstract]
Upchurch, B., Fung, B., Rindi, G, Ronco, A, Leiter, A. (1996). Peptide YY expression is an early event in colonic endocrine cell differentiation: evidence from normal and transgenic mice. Development 122: 1157-1163 [Abstract]
Wice, B. M., Gordon, J. I. (1995). A Tetraspan Membrane Glycoprotein Produced in the Human Intestinal Epithelium and Liver That Can Regulate Cell Density-dependent Proliferation. J. Biol. Chem. 270: 21907-21918 [Abstract] [Full Text]
Bisaha, J. G., Simon, T. C., Gordon, J. I., Breslow, J. L. (1995). Characterization of an Enhancer Element in the Human Apolipoprotein C-III Gene That Regulates Human Apolipoprotein A-I Gene Expression in the Intestinal Epithelium. J. Biol. Chem. 270: 19979-19988 [Abstract] [Full Text]
Li, Q., Karam, S. M., Gordon, J. I. (1995). Simian Virus 40 T Antigen-induced Amplification of Pre-parietal Cells in Transgenic Mice. J. Biol. Chem. 270: 15777-15788 [Abstract] [Full Text]
Lopez, M. J., Upchurch, B. H., Rindi, G., Leiter, A. B. (1995). Studies in Transgenic Mice Reveal Potential Relationships between Secretin-producing Cells and Other Endocrine Cell Types. J. Biol. Chem. 270: 885-891 [Abstract] [Full Text]