Subunit selectivity and epitope characterization of mAbs directed against the GABAA/benzodiazepine receptor

doi:10.1083/jcb.110.6.2043

This Article

	Full Text (PDF, 2643K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Ewert, M.
	Articles by Seeburg, P. H.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Ewert, M.
	Articles by Seeburg, P. H.

Social Bookmarking

	What's this?

ARTICLES

Subunit selectivity and epitope characterization of mAbs directed against the GABAA/benzodiazepine receptor

M Ewert, BD Shivers, H Luddens, H Mohler and PH Seeburg
Center for Molecular Biology, University of Heidelberg, FRG.

mAbs bd 17, bd 24, and bd 28 raised against bovine cerebral gamma- aminobutyric acid (GABAA)/benzodiazepine receptors were analyzed for their ability to detect each of 12 GABAA receptor subunits expressed in cultured mammalian cells. Results showed that mAb bd 17 recognizes epitopes on both beta 2 and beta 3 subunits while mAb bd 24 is selective for the alpha 1 subunit of human and bovine, but not of rat origin. The latter antibody reacts with the rat alpha 1 subunit carrying an engineered Leu at position four, documenting the first epitope mapping of a GABAA receptor subunit-specific mAb. In contrast to mAbs bd 17 and bd 24, mAb bd 28 reacts with all GABAA receptor subunits tested but not with a glycine receptor subunit, suggesting the presence of shared epitopes on subunits of GABA-gated chloride channels.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Loup, F., Picard, F., Yonekawa, Y., Wieser, H.-G., Fritschy, J.-M. (2009). Selective changes in GABAA receptor subtypes in white matter neurons of patients with focal epilepsy. Brain 132: 2449-2463 [Abstract] [Full Text]
Sarro, E. C., Kotak, V. C., Sanes, D. H., Aoki, C. (2008). Hearing Loss Alters the Subcellular Distribution of Presynaptic GAD and Postsynaptic GABAA Receptors in the Auditory Cortex. Cereb Cortex 18: 2855-2867 [Abstract] [Full Text]
Tippett, L. J., Waldvogel, H. J., Thomas, S. J., Hogg, V. M., Roon-Mom, W. v., Synek, B. J., Graybiel, A. M., Faull, R. L. M. (2007). Striosomes and mood dysfunction in Huntington's disease. Brain 130: 206-221 [Abstract] [Full Text]
Loup, F., Picard, F., Andre, V. M., Kehrli, P., Yonekawa, Y., Wieser, H.-G., Fritschy, J.-M. (2006). Altered expression of {alpha}3-containing GABAA receptors in the neocortex of patients with focal epilepsy. Brain 129: 3277-3289 [Abstract] [Full Text]
Chen, G., Kittler, J. T., Moss, S. J., Yan, Z. (2006). Dopamine D3 Receptors Regulate GABAA Receptor Function through a Phospho-Dependent Endocytosis Mechanism in Nucleus Accumbens. J. Neurosci. 26: 2513-2521 [Abstract] [Full Text]
Laschet, J. J., Minier, F., Kurcewicz, I., Bureau, M. H., Trottier, S., Jeanneteau, F., Griffon, N., Samyn, B., Van Beeumen, J., Louvel, J., Sokoloff, P., Pumain, R. (2004). Glyceraldehyde-3-Phosphate Dehydrogenase Is a GABAA Receptor Kinase Linking Glycolysis to Neuronal Inhibition. J. Neurosci. 24: 7614-7622 [Abstract] [Full Text]
Baumann, S. W., Baur, R., Sigel, E. (2003). Individual Properties of the Two Functional Agonist Sites in GABAA Receptors. J. Neurosci. 23: 11158-11166 [Abstract] [Full Text]
Sawyer, G. W., Chiara, D. C., Olsen, R. W., Cohen, J. B. (2002). Identification of the Bovine gamma -Aminobutyric Acid Type A Receptor alpha Subunit Residues Photolabeled by the Imidazobenzodiazepine [3H]Ro15-4513. J. Biol. Chem. 277: 50036-50045 [Abstract] [Full Text]
Baumann, S. W., Baur, R., Sigel, E. (2002). Forced Subunit Assembly in alpha 1beta 2gamma 2 GABAA Receptors. INSIGHT INTO THE ABSOLUTE ARRANGEMENT. J. Biol. Chem. 277: 46020-46025 [Abstract] [Full Text]
Klausberger, T., Roberts, J. D. B., Somogyi, P. (2002). Cell Type- and Input-Specific Differences in the Number and Subtypes of Synaptic GABAA Receptors in the Hippocampus. J. Neurosci. 22: 2513-2521 [Abstract] [Full Text]
Scotti, A. L., Reuter, H. (2001). Synaptic and extrasynaptic gamma -aminobutyric acid type A receptor clusters in rat hippocampal cultures during development. Proc. Natl. Acad. Sci. USA 10.1073/pnas.061028798v1 [Abstract] [Full Text]
Loup, F., Wieser, H.-G., Yonekawa, Y., Aguzzi, A., Fritschy, J.-M. (2000). Selective Alterations in GABAA Receptor Subtypes in Human Temporal Lobe Epilepsy. J. Neurosci. 20: 5401-5419 [Abstract] [Full Text]
Lim, R., Alvarez, F. J, Walmsley, B. (2000). GABA mediates presynaptic inhibition at glycinergic synapses in a rat auditory brainstem nucleus. J. Physiol. 525: 447-459 [Abstract] [Full Text]
Kannenberg, K., Schaerer, M. T., Fuchs, K., Sieghart, W., Sigel, E. (1999). A Novel Serine Kinase with Specificity for beta 3-Subunits Is Tightly Associated with GABAA Receptors. J. Biol. Chem. 274: 21257-21264 [Abstract] [Full Text]
Chapell, R., Bueno, O. F., Alvarez-Hernandez, X., Robinson, L. C., Leidenheimer, N. J. (1998). Activation of Protein Kinase C Induces gamma -Aminobutyric Acid Type A Receptor Internalization in Xenopus Oocytes. J. Biol. Chem. 273: 32595-32601 [Abstract] [Full Text]
Loup, F., Weinmann, O., Yonekawa, Y., Aguzzi, A., Wieser, H.-G., Fritschy, J.-M. (1998). A Highly Sensitive Immunofluorescence Procedure for Analyzing the Subcellular Distribution of GABAA Receptor Subunits in the Human Brain. J. Histochem. Cytochem. 46: 1129-1140 [Abstract] [Full Text]
Rao, A., Kim, E., Sheng, M., Craig, A. M. (1998). Heterogeneity in the Molecular Composition of Excitatory Postsynaptic Sites during Development of Hippocampal Neurons in Culture. J. Neurosci. 18: 1217-1229 [Abstract] [Full Text]
Levi, S, Vannier, C, Triller, A (1998). Strychnine-sensitive stabilization of postsynaptic glycine receptor clusters. J. Cell Sci. 111: 335-345 [Abstract]
Wan, Q., Man, H. Y., Braunton, J., Wang, W., Salter, M. W., Becker, L., Wang, Y. T. (1997). Modulation of GABAA Receptor Function by Tyrosine Phosphorylation of beta �Subunits. J. Neurosci. 17: 5062-5069 [Abstract] [Full Text]
Craig, A. M., Banker, G., Chang, W., McGrath, M. E., Serpinskaya, A. S. (1996). Clustering of Gephyrin at GABAergic but Not Glutamatergic Synapses in Cultured Rat Hippocampal Neurons. J. Neurosci. 16: 3166-3177 [Abstract] [Full Text]
Pollard, S., Thompson, C. L., Stephenson, F. A. (1995). Quantitative Characterization of alpha6 and alpha1alpha6 Subunit-containing Native [IMAGE]-Aminobutyric Acid(A) Receptors of Adult Rat Cerebellum Demonstrates Two alpha Subunits per Receptor Oligomer. J. Biol. Chem. 270: 21285-21290 [Abstract] [Full Text]
Seeburg, P.H., Wisden, W., Verdoorn, T.A., Pritchett, D.B., Werner, P., Herb, A., Luddens, H., Sprengel, R., Sakmann, B. (1990). The GABAA Receptor Family: Molecular and Functional Diversity. Cold Spring Harb Symp Quant Biol 55: 29-40 [Abstract]
Klausberger, T., Ehya, N., Fuchs, K., Fuchs, T., Ebert, V., Sarto, I., Sieghart, W. (2001). Detection and Binding Properties of GABAA Receptor Assembly Intermediates. J. Biol. Chem. 276: 16024-16032 [Abstract] [Full Text]
Schaerer, M. T., Kannenberg, K., Hunziker, P., Baumann, S. W., Sigel, E. (2001). Interaction between GABAA Receptor beta Subunits and the Multifunctional Protein gC1q-R. J. Biol. Chem. 276: 26597-26604 [Abstract] [Full Text]
Baumann, S. W., Baur, R., Sigel, E. (2001). Subunit Arrangement of gamma -Aminobutyric Acid Type A Receptors. J. Biol. Chem. 276: 36275-36280 [Abstract] [Full Text]
Scotti, A. L., Reuter, H. (2001). Synaptic and extrasynaptic gamma -aminobutyric acid type A receptor clusters in rat hippocampal cultures during development. Proc. Natl. Acad. Sci. USA 98: 3489-3494 [Abstract] [Full Text]