The Journal of Cell Biology, Vol 110, 2209-2219, Copyright © 1990 by The Rockefeller University Press

ARTICLES

Epithelium-dependent extracellular matrix synthesis in transforming growth factor-beta 1-growth-inhibited mouse mammary gland

GB Silberstein, P Strickland, S Coleman and CW Daniel
Department of Biology, Sinsheimer Laboratories, University of California, Santa Cruz 95064.

Exogenous transforming growth factor beta (TGF-beta 1) was shown in earlier studies to reversibly inhibit mouse mammary ductal growth. Using small plastic implants to treat regions of developing mammary glands in situ, we now report that TGF-beta 1 growth inhibition is associated with an ectopic accumulation of type I collagen messenger RNA and protein, as well as the glycosaminoglycan, chondroitin sulfate. Both macromolecules are normal components of the ductal extracellular matrix, which, under the influence of exogenous TGF-beta 1, became unusually concentrated immediately adjacent to the epithelial cells at the tip of the ductal growth points, the end buds. Stimulation of extracellular matrix was confined to aggregations of connective tissue cells around affected end buds and was not present around the TGF-beta 1 implants themselves, indicating that the matrix effect was epithelium dependent. Ectopic matrix synthesis was specific for TGF-beta 1 insofar as it was absent at ducts treated with other growth inhibitors, or at ducts undergoing normal involution in response to endogenous regulatory processes. These findings are consistent with the matrix-stimulating properties of TGF-beta 1 reported for other systems, but differ in their strict dependence upon epithelium. A possible role for endogenous TGF-beta 1 in modulating a mammary epithelium-stroma interaction is suggested.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Facebook    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

• Musters, S., Coughlan, K., McFadden, T., Maple, R., Mulvey, T., Plaut, K. (2004). Exogenous TGF-{beta}1 Promotes Stromal Development in the Heifer Mammary Gland. J DAIRY SCI 87: 896-904 [Abstract] [Full Text]  
• Berry, S. D. K., Howard, R. D., Akers, R. M. (2003). Mammary Localization and Abundance of Laminin, Fibronectin, and Collagen IV Proteins in Prepubertal Heifers. J DAIRY SCI 86: 2864-2874 [Abstract] [Full Text]  
• Plaut, K., Dean, A. J., Patnode, T. A., Casey, T. M. (2003). Effect of Transforming Growth Factor-beta (TGF-{beta}) on Mammary Development. J DAIRY SCI 86: E16-27 [Abstract] [Full Text]  
• Koblinski, J. E., Dosescu, J., Sameni, M., Moin, K., Clark, K., Sloane, B. F. (2002). Interaction of Human Breast Fibroblasts with Collagen I Increases Secretion of Procathepsin B. J. Biol. Chem. 277: 32220-32227 [Abstract] [Full Text]  
• Boland, S, Boisvieux-Ulrich, E, Houcine, O, Baeza-Squiban, A, Pouchelet, M, Schoevaert, D, Marano, F (1996). TGF beta 1 promotes actin cytoskeleton reorganization and migratory phenotype in epithelial tracheal cells in primary culture. J. Cell Sci. 109: 2207-2219 [Abstract]  
• Niranjan, B, Buluwela, L, Yant, J, Perusinghe, N, Atherton, A, Phippard, D, Dale, T, Gusterson, B, Kamalati, T (1995). HGF/SF: a potent cytokine for mammary growth, morphogenesis and development. Development 121: 2897-2908 [Abstract]  
• Pierce, D F, Johnson, M D, Matsui, Y, Robinson, S D, Gold, L I, Purchio, A F, Daniel, C W, Hogan, B L, Moses, H L (1993). Inhibition of mammary duct development but not alveolar outgrowth during pregnancy in transgenic mice expressing active TGF-beta 1.. Genes Dev. 7: 2308-2317 [Abstract]  

Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents