A homing receptor-IgG chimera as a probe for adhesive ligands of lymph node high endothelial venules

doi:10.1083/jcb.110.6.2221

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A homing receptor-IgG chimera as a probe for adhesive ligands of lymph node high endothelial venules

SR Watson, Y Imai, C Fennie, JS Geoffroy, SD Rosen and LA Lasky
Department of Immunobiology, Genentech, Inc., South San Francisco, California 94080.

The binding of lymphocytes to high endothelial venules (HEV) within peripheral lymph nodes (pln) is thought to be mediated by a lectinlike adhesion molecule termed the pln homing receptor (pln HR). The cloning and sequencing of cDNAs encoding both murine and human pln HR revealed that these adhesion molecules contain protein motifs that are homologous to C-type or calcium dependent lectin domains as well as to epidermal growth factor (egf) and complement-regulatory protein domains. We have produced a novel, antibody-like form of the murine HR by joining the extracellular region of the receptor to a human IgG heavy chain. This antibody-like molecule is capable of recognizing carbohydrates, blocking the binding of lymphocytes to pln HEV, and serving as a histochemical reagent for the staining of pln HEV. This murine HR-IgG chimera should prove useful in analyzing the distribution of the HR ligand(s) in normal as well as in inflammatory states.
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