Myosin II heavy chain null mutant of Dictyostelium exhibits defective intracellular particle movement

doi:10.1083/jcb.111.3.1137

This Article

	Full Text (PDF, 2434K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Wessels, D.
	Articles by Soll, D. R.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Wessels, D.
	Articles by Soll, D. R.


Social Bookmarking

	What's this?

ARTICLES

Myosin II heavy chain null mutant of Dictyostelium exhibits defective intracellular particle movement

D Wessels and DR Soll
Department of Biology, University of Iowa, Iowa City 52242.

Both cellular motility and intracellular particle movement are compared between normal Dictyostelium amebae of strain AX4 and amebae of a myosin II heavy chain null mutant, HS2215, using the computer assisted "Dynamic Morphology System." In AX4 cells rapidly translocating in buffer, cytoplasmic expansion is apical and the majority of intracellular particles move anteriorly, towards the site of expansion. When these cells are pulsed with 10(-6) M cAMP, the peak concentration of the natural cAMP wave, cells stop translocating and average particle velocity decreases threefold within 2-4 s after cAMP addition. After 8 s, there is a partial rebound both in cytoplasmic expansion and particle velocity, but in both cases, original apical polarity is lost. In HS2215 cells in buffer, both cellular translocation and average particle velocity are already at the depressed levels observed in normal cells immediately after cAMP addition, and no anterior bias is observed in either the direction of cytoplasmic expansion or the direction of particle movement. The addition of cAMP to myosin-minus cells results in no additional effect. The results demonstrate that myosin II is necessary for (a) the rapid rate of intracellular particle movement, (b) the biased anterior directionality of particle movement, and (c) the rapid inhibition of particle movement by cAMP.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Mondal, S., Bakthavatsalam, D., Steimle, P., Gassen, B., Rivero, F., Noegel, A. A. (2008). Linking Ras to myosin function: RasGEF Q, a Dictyostelium exchange factor for RasB, affects myosin II functions. JCB 181: 747-760 [Abstract] [Full Text]
Wessels, D., Lusche, D. F., Kuhl, S., Heid, P., Soll, D. R. (2007). PTEN plays a role in the suppression of lateral pseudopod formation during Dictyostelium motility and chemotaxis. J. Cell Sci. 120: 2517-2531 [Abstract] [Full Text]
Bellion, A., Baudoin, J.-P., Alvarez, C., Bornens, M., Metin, C. (2005). Nucleokinesis in Tangentially Migrating Neurons Comprises Two Alternating Phases: Forward Migration of the Golgi/Centrosome Associated with Centrosome Splitting and Myosin Contraction at the Rear. J. Neurosci. 25: 5691-5699 [Abstract] [Full Text]
Egelhoff, T. T., Croft, D., Steimle, P. A. (2005). Actin Activation of Myosin Heavy Chain Kinase A in Dictyostelium: A BIOCHEMICAL MECHANISM FOR THE SPATIAL REGULATION OF MYOSIN II FILAMENT DISASSEMBLY. J. Biol. Chem. 280: 2879-2887 [Abstract] [Full Text]
Totsukawa, G., Wu, Y., Sasaki, Y., Hartshorne, D. J., Yamakita, Y., Yamashiro, S., Matsumura, F. (2004). Distinct roles of MLCK and ROCK in the regulation of membrane protrusions and focal adhesion dynamics during cell migration of fibroblasts. JCB 164: 427-439 [Abstract] [Full Text]
Kolega, J. (2003). Asymmetric Distribution of Myosin IIB in Migrating Endothelial Cells Is Regulated by a rho-dependent Kinase and Contributes to Tail Retraction. Mol. Biol. Cell 14: 4745-4757 [Abstract] [Full Text]
Zhang, H., Wessels, D., Fey, P., Daniels, K., Chisholm, R. L., Soll, D. R. (2002). Phosphorylation of the myosin regulatory light chain plays a role in motility and polarity during Dictyostelium chemotaxis. J. Cell Sci. 115: 1733-1747 [Abstract] [Full Text]
Fukui, R., Amakawa, M., Hoshiga, M., Shibata, N., Kohbayashi, E., Seto, M., Sasaki, Y., Ueno, T., Negoro, N., Nakakoji, T., Ii, M., Nishiguchi, F., Ishihara, T., Ohsawa, N. (2000). Increased migration in late G1 phase in cultured smooth muscle cells. Am. J. Physiol. Cell Physiol. 279: C999-C1007 [Abstract] [Full Text]
Wessels, D. J., Zhang, H., Reynolds, J., Daniels, K., Heid, P., Lu, S., Kuspa, A., Shaulsky, G., Loomis, W. F., Soll, D. R. (2000). The Internal Phosphodiesterase RegA Is Essential for the Suppression of Lateral Pseudopods during Dictyostelium Chemotaxis. Mol. Biol. Cell 11: 2803-2820 [Abstract] [Full Text]
Wessels, D, Reynolds, J, Johnson, O, Voss, E, Burns, R, Daniels, K, Garrard, E, O'Halloran, T., Soll, D. (2000). Clathrin plays a novel role in the regulation of cell polarity, pseudopod formation, uropod stability and motility in Dictyostelium. J. Cell Sci. 113: 21-36 [Abstract]
Chung, C. Y., Firtel, R. A. (1999). Paka, a Putative Pak Family Member, Is Required for Cytokinesis and the Regulation of the Cytoskeleton in Dictyostelium discoideum Cells during Chemotaxis. JCB 147: 559-576 [Abstract] [Full Text]
Rivero, F., Kuspa, A., Brokamp, R., Matzner, M., Noegel, A. A. (1998). Interaptin, an Actin-binding Protein of the {alpha}-Actinin Superfamily in Dictyostelium discoideum, Is Developmentally and cAMP-regulated and Associates with Intracellular Membrane Compartments. JCB 142: 735-750 [Abstract] [Full Text]
Kolega, J (1998). Cytoplasmic dynamics of myosin IIA and IIB: spatial 'sorting' of isoforms in locomoting cells. J. Cell Sci. 111: 2085-2095 [Abstract]
Sylwester, A, Wessels, D, Anderson, S., Warren, R., Shutt, D., Kennedy, R., Soll, D. (1993). HIV-induced syncytia of a T cell line form single giant pseudopods and are motile. J. Cell Sci. 106: 941-953 [Abstract]