Myogenic programs of mouse muscle cell lines: expression of myosin heavy chain isoforms, MyoD1, and myogenin

doi:10.1083/jcb.111.3.1149

This Article

	Full Text (PDF, 2007K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Miller, J. B.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Miller, J. B.


Social Bookmarking

	What's this?

ARTICLES

Myogenic programs of mouse muscle cell lines: expression of myosin heavy chain isoforms, MyoD1, and myogenin

JB Miller
Cecil B. Day Laboratory for Neuromuscular Research, Massachusetts General Hospital, Charlestown 02129.

Different mouse muscle cell lines were found to express distinct patterns of myosin heavy chain (MHC) isoforms, MyoD1, and myogenin, but there appeared to be no correlation between the pattern of MHC expression and the patterns of MyoD1 and myogenin expression. Myogenic cell lines were generated from unconverted C3H10T1/2 cells by 5- azacytidine treatment (Aza cell lines) and by stable transfection with MyoD1 (TD cell lines) or myogenin (TG cell lines). Myogenic differentiation of the newly generated cell lines was compared to that of the C2C12 and BC3H-1 cell lines. Immunoblot analysis showed that differentiated cells of each line expressed the embryonic and slow skeletal/beta-cardiac MHC isoforms though slow MHC was expressed at a much lower, barely detectable level in BC3H-1 cells. Differentiated cells of each line except BC3H-1 also expressed an additional MHC(s) that was probably the perinatal MHC isoform. Myogenin mRNA was expressed by every cell line, and, with the exception of BC3H-1 (cf., Davis, R. L., H. Weintraub, and A. B. Lassar. 1987. Cell. 51:987-1000), MyoD1 mRNA was expressed by every cell line. To determine if MyoD1 expression would alter the differentiation of BC3H-1 cells, cell lines (termed BD) were generated by transfecting BC3H-1 cells with MyoD1 under control of the beta-actin promoter. The MyoD1 protein expressed in BD cells was correctly localized in the nucleus, and, unlike the parental BC3H-1 cell line that formed differentiated MHC-expressing cells, which were predominantly mononucleated, BD cell lines formed long, multinucleated myotubes (cf., Brennan, T. J., D. G. Edmondson, and E. N. Olson. 1990. J. Cell. Biol. 110:929-938). Despite the differences in morphology and MyoD1 expression, BD myotubes and the parent BC3H-1 cells expressed the same pattern of sarcomeric MHCs.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Bryan, B. A., Walshe, T. E., Mitchell, D. C., Havumaki, J. S., Saint-Geniez, M., Maharaj, A. S., Maldonado, A. E., D'Amore, P. A. (2008). Coordinated Vascular Endothelial Growth Factor Expression and Signaling During Skeletal Myogenic Differentiation. Mol. Biol. Cell 19: 994-1006 [Abstract] [Full Text]
Watanabe, S., Kondo, S., Hayasaka, M., Hanaoka, K. (2007). Functional analysis of homeodomain-containing transcription factor Lbx1 in satellite cells of mouse skeletal muscle. J. Cell Sci. 120: 4178-4187 [Abstract] [Full Text]
Chandran, R., Knobloch, T. J., Anghelina, M., Agarwal, S. (2007). Biomechanical signals upregulate myogenic gene induction in the presence or absence of inflammation. Am. J. Physiol. Cell Physiol. 293: C267-C276 [Abstract] [Full Text]
Cottle, D. L., McGrath, M. J., Cowling, B. S., Coghill, I. D., Brown, S., Mitchell, C. A. (2007). FHL3 binds MyoD and negatively regulates myotube formation. J. Cell Sci. 120: 1423-1435 [Abstract] [Full Text]
Wang, X., Huang, Q.-Q., Breckenridge, M. T., Chen, A., Crawford, T. O., Morton, D. H., Jin, J.-P. (2005). Cellular Fate of Truncated Slow Skeletal Muscle Troponin T Produced by Glu180 Nonsense Mutation in Amish Nemaline Myopathy. J. Biol. Chem. 280: 13241-13249 [Abstract] [Full Text]
Zebedin, E., Sandtner, W., Galler, S., Szendroedi, J., Just, H., Todt, H., Hilber, K. (2004). Fiber type conversion alters inactivation of voltage-dependent sodium currents in murine C2C12 skeletal muscle cells. Am. J. Physiol. Cell Physiol. 287: C270-C280 [Abstract] [Full Text]
McGrath, M. J., Mitchell, C. A., Coghill, I. D., Robinson, P. A., Brown, S. (2003). Skeletal muscle LIM protein 1 (SLIM1/FHL1) induces {alpha}5{beta}1-integrin-dependent myocyte elongation. Am. J. Physiol. Cell Physiol. 285: C1513-C1526 [Abstract] [Full Text]
Shuler, C.F., Dalrymple, K.R. (2001). Molecular Regulation of Tongue and Craniofacial Muscle Differentiation. CROBM 12: 3-17 [Abstract] [Full Text]
Ridgeway, A. G., Wilton, S., Skerjanc, I. S. (2000). Myocyte Enhancer Factor 2C and Myogenin Up-regulate Each Other's Expression and Induce the Development of Skeletal Muscle in P19 Cells. J. Biol. Chem. 275: 41-46 [Abstract] [Full Text]
Sabourin, L. A., Girgis-Gabardo, A., Seale, P., Asakura, A., Rudnicki, M. A. (1999). Reduced Differentiation Potential of Primary MyoD-/- Myogenic Cells Derived from Adult Skeletal Muscle. JCB 144: 631-643 [Abstract] [Full Text]
Cooper, R., Tajbakhsh, S, Mouly, V, Cossu, G, Buckingham, M, Butler-Browne, G. (1999). In vivo satellite cell activation via Myf5 and MyoD in regenerating mouse skeletal muscle. J. Cell Sci. 112: 2895-2901 [Abstract]
Dominov, J. A., Dunn, J. J., Miller, J. B. (1998). Bcl-2 Expression Identifies an Early Stage of Myogenesis and Promotes Clonal Expansion of Muscle Cells. JCB 142: 537-544 [Abstract] [Full Text]
Meyers, M. B., Puri, T. S., Chien, A. J., Gao, T., Hsu, P.-H., Hosey, M. M., Fishman, G. I. (1998). Sorcin Associates with the Pore-forming Subunit of Voltage-dependent L-type Ca2+ Channels. J. Biol. Chem. 273: 18930-18935 [Abstract] [Full Text]
Yoshida, N, Yoshida, S, Koishi, K, Masuda, K, Nabeshima, Y (1998). Cell heterogeneity upon myogenic differentiation: down-regulation of MyoD and Myf-5 generates 'reserve cells'. J. Cell Sci. 111: 769-779 [Abstract]
Mangan, M., Olmsted, J. (1996). A muscle-specific variant of microtubule-associated protein 4 (MAP4) is required in myogenesis. Development 122: 771-781 [Abstract]
Breitbart, R., Liang, C., Smoot, L., Laheru, D., Mahdavi, V, Nadal-Ginard, B (1993). A fourth human MEF2 transcription factor, hMEF2D, is an early marker of the myogenic lineage. Development 118: 1095-1106 [Abstract]
Hughes, S., Taylor, J., Tapscott, S., Gurley, C., Carter, W., Peterson, C. (1993). Selective accumulation of MyoD and myogenin mRNAs in fast and slow adult skeletal muscle is controlled by innervation and hormones. Development 118: 1137-1147 [Abstract]
Smith, T., Block, N., Rhodes, S., Konieczny, S., Miller, J. (1993). A unique pattern of expression of the four muscle regulatory factor proteins distinguishes somitic from embryonic, fetal and newborn mouse myogenic cells. Development 117: 1125-1133 [Abstract]
Donoghue, M. J., Patton, B. L., Sanes, J. R., Merlie, J. P. (1992). An axial gradient of transgene methylation in murine skeletal muscle: genomic imprint of rostrocaudal position. Development 116: 1101-1112 [Abstract]
Yu, Y T, Breitbart, R E, Smoot, L B, Lee, Y, Mahdavi, V, Nadal-Ginard, B (1992). Human myocyte-specific enhancer factor 2 comprises a group of tissue-restricted MADS box transcription factors.. Genes Dev. 6: 1783-1798 [Abstract]