Direct demonstration of the lectin activity of gp90MEL, a lymphocyte homing receptor

doi:10.1083/jcb.111.3.1225

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Direct demonstration of the lectin activity of gp90MEL, a lymphocyte homing receptor

Y Imai, DD True, MS Singer and SD Rosen
Department of Anatomy, University of California, San Francisco 94143- 0452.

Considerable evidence implicates gp90MEL as a lymphocyte homing receptor mediating lymphocyte attachment to high endothelial venules of lymph nodes in mouse. The protein appears to function as a calcium- dependent, lectin-like receptor as inferred primarily by the ability of specific carbohydrates to block its function and by the presence of a calcium-type lectin domain in its primary sequence. An ELISA assay is described which provides the first demonstration that the isolated protein has lectin activity and allows a further definition of its carbohydrate specificity. In addition to the monosaccharides mannose-6- phosphate and fructose-1-phosphate, ligand activity is shown for the sulfated glycolipid, sulfatide, and for two sulfated fucose-containing polysaccharides (fucoidin and egg jelly coat) from nonmammalian sources.
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