Human microvascular endothelial cells use beta 1 and beta 3 integrin receptor complexes to attach to laminin

doi:10.1083/jcb.111.3.1233

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Human microvascular endothelial cells use beta 1 and beta 3 integrin receptor complexes to attach to laminin

RH Kramer, YF Cheng and R Clyman
Department of Stomatology, University of California, San Francisco 94143-0512.

Microvascular endothelial cells (MEC) use a set of surface receptors to adhere not only to the vascular basement membrane but, during angiogenic stimulation, to the interstitium. We examined how cultured human MEC interact with laminin-rich basement membranes. By using a panel of monoclonal antibodies, we found that MEC cells express a number of integrin-related receptor complexes, including alpha 1 beta 1, alpha 2 beta 1, alpha 3 beta 1, alpha 5 beta 1, alpha 6 beta 1, alpha V beta 3. Attachment to laminin, a major adhesive protein in basement membranes, was studied in detail. Blocking monoclonal antibodies specific to different integrin receptor complexes showed that the alpha 6 beta 1 complex was important for MEC adhesion to laminin. In addition, blocking antibody also implicated the vitronectin receptor (alpha V beta 3) in laminin adhesion. We used ligand affinity chromatography of detergent-solubilized receptor complexes to further define receptor specificity. On laminin-Sepharose columns, we identified several integrin receptor complexes whose affinity for the ligand was dependent on the type of divalent cation present. Several beta 1 complexes, including alpha 1 beta 1, alpha 2 beta 1, and alpha 6 beta 1 bound strongly to laminin. In agreement with the antibody blocking experiments, alpha V beta 3 was found to bind well to laminin. However, unlike binding to its other ligands (e.g., vitronectin, fibrinogen, von Willebrand factor), alpha V beta 3 interaction with laminin did not appear to be Arg-Gly-Asp (RGD) sensitive. Finally, immunofluorescent staining demonstrated both beta 1 and beta 3 complexes in vinculin-positive focal adhesion plaques on the basal surface of MEC adhering to laminin-coated substrates. The results indicate that both these subfamilies of integrin heterodimers are involved in promoting MEC adhesion to laminin and the vascular basement membrane.
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Desban, N, Duband, J. (1997). Avian neural crest cell migration on laminin: interaction of the alpha1beta1 integrin with distinct laminin-1 domains mediates different adhesive responses. J. Cell Sci. 110: 2729-2744 [Abstract]
Schaefer, T, Roux, M, Stuhlsatz, H., Herken, R, Coulomb, B, Krieg, T, Smola, H (1996). Glycosaminoglycans modulate cell-matrix interactions of human fibroblasts and endothelial cells in vitro. J. Cell Sci. 109: 479-488 [Abstract]
Cattelino, A, Longhi, R, de Curtis, I (1995). Differential distribution of two cytoplasmic variants of the alpha 6 beta 1 integrin laminin receptor in the ventral plasma membrane of embryonic fibroblasts. J. Cell Sci. 108: 3067-3078 [Abstract]
Tozeren, A, Kleinman, H., Wu, S, Mercurio, A., Byers, S. (1994). Integrin alpha 6 beta 4 mediates dynamic interactions with laminin. J. Cell Sci. 107: 3153-3163 [Abstract]
Hara, M, Yaar, M, Tang, A, Eller, M., Reenstra, W, Gilchrest, B. (1994). Role of integrins in melanocyte attachment and dendricity. J. Cell Sci. 107: 2739-2748 [Abstract]
Clyman, R., Tannenbaum, J, Chen, Y., Cooper, D, Yurchenco, P., Kramer, R., Waleh, N. (1994). Ductus arteriosus smooth muscle cell migration on collagen: dependence on laminin and its receptors. J. Cell Sci. 107: 1007-1018 [Abstract]
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