Distinct transport vesicles mediate the delivery of plasma membrane proteins to the apical and basolateral domains of MDCK cells

doi:10.1083/jcb.111.3.987

This Article

	Full Text (PDF, 3050K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Wandinger-Ness, A.
	Articles by Simons, K.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Wandinger-Ness, A.
	Articles by Simons, K.


Social Bookmarking

	What's this?

ARTICLES

Distinct transport vesicles mediate the delivery of plasma membrane proteins to the apical and basolateral domains of MDCK cells

A Wandinger-Ness, MK Bennett, C Antony and K Simons
Cell Biology Program, European Molecular Biology Laboratory, Heidelberg, Federal Republic of Germany.

Immunoisolation techniques have led to the purification of apical and basolateral transport vesicles that mediate the delivery of proteins from the trans-Golgi network to the two plasma membrane domains of MDCK cells. We showed previously that these transport vesicles can be formed and released in the presence of ATP from mechanically perforated cells (Bennett, M. K., A. Wandinger-Ness, and K. Simons, 1988. EMBO (Euro. Mol. Biol. Organ.) J. 7:4075-4085). Using virally infected cells, we have monitored the purification of the trans-Golgi derived vesicles by following influenza hemagglutinin or vesicular stomatitis virus (VSV) G protein as apical and basolateral markers, respectively. Equilibrium density gradient centrifugation revealed that hemagglutinin containing vesicles had a slightly lower density than those containing VSV-G protein, indicating that the two fractions were distinct. Antibodies directed against the cytoplasmically exposed domains of the viral spike glycoproteins permitted the resolution of apical and basolateral vesicle fractions. The immunoisolated vesicles contained a subset of the proteins present in the starting fraction. Many of the proteins were sialylated as expected for proteins existing the trans-Golgi network. The two populations of vesicles contained a number of proteins in common, as well as components which were enriched up to 38-fold in one fraction relative to the other. Among the unique components, a number of transmembrane proteins could be identified using Triton X-114 phase partitioning. This work provides evidence that two distinct classes of vesicles are responsible for apical and basolateral protein delivery. Common protein components are suggested to be involved in vesicle budding and fusion steps, while unique components may be required for specific recognition events such as those involved in protein sorting and vesicle targeting.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Farr, G. A., Hull, M., Mellman, I., Caplan, M. J. (2009). Membrane proteins follow multiple pathways to the basolateral cell surface in polarized epithelial cells. JCB 186: 269-282 [Abstract] [Full Text]
Klemm, R. W., Ejsing, C. S., Surma, M. A., Kaiser, H.-J., Gerl, M. J., Sampaio, J. L., de Robillard, Q., Ferguson, C., Proszynski, T. J., Shevchenko, A., Simons, K. (2009). Segregation of sphingolipids and sterols during formation of secretory vesicles at the trans-Golgi network. JCB 185: 601-612 [Abstract] [Full Text]
Henry, L., Sheff, D. R. (2008). Rab8 Regulates Basolateral Secretory, But Not Recycling, Traffic at the Recycling Endosome. Mol. Biol. Cell 19: 2059-2068 [Abstract] [Full Text]
Spiliotis, E. T., Hunt, S. J., Hu, Q., Kinoshita, M., Nelson, W. J. (2008). Epithelial polarity requires septin coupling of vesicle transport to polyglutamylated microtubules. JCB 180: 295-303 [Abstract] [Full Text]
Pocard, T., Le Bivic, A., Galli, T., Zurzolo, C. (2007). Distinct v-SNAREs regulate direct and indirect apical delivery in polarized epithelial cells. J. Cell Sci. 120: 3309-3320 [Abstract] [Full Text]
Paladino, S., Pocard, T., Catino, M. A., Zurzolo, C. (2006). GPI-anchored proteins are directly targeted to the apical surface in fully polarized MDCK cells. JCB 172: 1023-1034 [Abstract] [Full Text]
Delacour, D., Gouyer, V., Zanetta, J.-P., Drobecq, H., Leteurtre, E., Grard, G., Moreau-Hannedouche, O., Maes, E., Pons, A., Andre, S., Le Bivic, A., Gabius, H. J., Manninen, A., Simons, K., Huet, G. (2005). Galectin-4 and sulfatides in apical membrane trafficking in enterocyte-like cells. JCB 169: 491-501 [Abstract] [Full Text]
Ellis, M. A., Miedel, M. T., Guerriero, C. J., Weisz, O. A. (2004). ADP-ribosylation Factor 1-independent Protein Sorting and Export from the trans-Golgi Network. J. Biol. Chem. 279: 52735-52743 [Abstract] [Full Text]
Paladino, S., Sarnataro, D., Pillich, R., Tivodar, S., Nitsch, L., Zurzolo, C. (2004). Protein oligomerization modulates raft partitioning and apical sorting of GPI-anchored proteins. JCB 167: 699-709 [Abstract] [Full Text]
Ang, A. L., Taguchi, T., Francis, S., Folsch, H., Murrells, L. J., Pypaert, M., Warren, G., Mellman, I. (2004). Recycling endosomes can serve as intermediates during transport from the Golgi to the plasma membrane of MDCK cells. JCB 167: 531-543 [Abstract] [Full Text]
Murphy, S. J., Dore, J. J. E., Edens, M., Coffey, R. J., Barnard, J. A., Mitchell, H., Wilkes, M., Leof, E. B. (2004). Differential Trafficking of Transforming Growth Factor-{beta} Receptors and Ligand in Polarized Epithelial Cells. Mol. Biol. Cell 15: 2853-2862 [Abstract] [Full Text]
Rosin, D., Schejter, E., Volk, T., Shilo, B.-Z. (2004). Apical accumulation of the Drosophila PDGF/VEGF receptor ligands provides a mechanism for triggering localized actin polymerization. Development 131: 1939-1948 [Abstract] [Full Text]
Wisco, D., Anderson, E. D., Chang, M. C., Norden, C., Boiko, T., Folsch, H., Winckler, B. (2003). Uncovering multiple axonal targeting pathways in hippocampal neurons. JCB 162: 1317-1328 [Abstract] [Full Text]
Delacour, D., Gouyer, V., Leteurtre, E., Ait-Slimane, T., Drobecq, H., Lenoir, C., Moreau-Hannedouche, O., Trugnan, G., Huet, G. (2003). 1-Benzyl-2-acetamido-2-deoxy-{alpha}-D-galactopyranoside Blocks the Apical Biosynthetic Pathway in Polarized HT-29 Cells. J. Biol. Chem. 278: 37799-37809 [Abstract] [Full Text]
Grant, K. S., Wira, C. R. (2003). Effect of Mouse Uterine Stromal Cells on Epithelial Cell Transepithelial Resistance (TER) and TNF{alpha} and TGF{beta} Release in Culture. Biol. Reprod. 69: 1091-1098 [Abstract] [Full Text]
Huber, L. A., Pfaller, K., Vietor, I. (2003). Organelle Proteomics: Implications for Subcellular Fractionation in Proteomics. Circ. Res. 92: 962-968 [Abstract] [Full Text]
Maier, O., Hoekstra, D. (2003). Trans-Golgi Network and Subapical Compartment of HepG2 Cells Display Different Properties in Sorting and Exiting of Sphingolipids. J. Biol. Chem. 278: 164-173 [Abstract] [Full Text]
Tuma, P. L., Nyasae, L. K., Hubbard, A. L. (2002). Nonpolarized Cells Selectively Sort Apical Proteins from Cell Surface to a Novel Compartment, but Lack Apical Retention Mechanisms. Mol. Biol. Cell 13: 3400-3415 [Abstract] [Full Text]
Luo, C., Nobusawa, E., Nakajima, K. (2002). Analysis of the desialidation process of the haemagglutinin protein of influenza B virus: the host-dependent desialidation step. J. Gen. Virol. 83: 1729-1734 [Abstract] [Full Text]
Lipardi, C., Ruggiano, G., Perrone, L., Paladino, S., Monlauzeur, L., Nitsch, L., Le Bivic, A., Zurzolo, C. (2002). Differential Recognition of a Tyrosine-Dependent Signal in the Basolateral and Endocytic Pathways of Thyroid Epithelial Cells. Endocrinology 143: 1291-1301 [Abstract] [Full Text]
Alfalah, M., Jacob, R., Naim, H. Y. (2002). Intestinal Dipeptidyl Peptidase IV Is Efficiently Sorted to the Apical Membrane through the Concerted Action of N- and O-Glycans as Well as Association with Lipid Microdomains. J. Biol. Chem. 277: 10683-10690 [Abstract] [Full Text]
Mora, R., Rodriguez-Boulan, E., Palese, P., Garcia-Sastre, A. (2002). Apical Budding of a Recombinant Influenza A Virus Expressing a Hemagglutinin Protein with a Basolateral Localization Signal. J. Virol. 76: 3544-3553 [Abstract] [Full Text]
Dahm, T., White, J., Grill, S., Füllekrug, J., Stelzer, E. H.K. (2001). Quantitative ER {left-right-arrow} Golgi Transport Kinetics and Protein Separation upon Golgi Exit Revealed by Vesicular Integral Membrane Protein 36 Dynamics in Live Cells. Mol. Biol. Cell 12: 1481-1498 [Abstract] [Full Text]
Martín-Belmonte, F., Puertollano, R., Millán, J., Alonso, M. A. (2000). The MAL Proteolipid Is Necessary for the Overall Apical Delivery of Membrane Proteins in the Polarized Epithelial Madin-Darby Canine Kidney and Fischer Rat Thyroid Cell Lines. Mol. Biol. Cell 11: 2033-2045 [Abstract] [Full Text]
Charron, A. J., Nakamura, S., Bacallao, R., Wandinger-Ness, A. (2000). Compromised Cytoarchitecture and Polarized Trafficking in Autosomal Dominant Polycystic Kidney Disease Cells. JCB 149: 111-124 [Abstract] [Full Text]
Renold, A., Cescato, R., Beuret, N., Vogel, L. K., Wahlberg, J. M., Brown, J. L., Fiedler, K., Spiess, M. (2000). Basolateral Sorting Signals Differ in Their Ability to Redirect Apical Proteins to the Basolateral Cell Surface. J. Biol. Chem. 275: 9290-9295 [Abstract] [Full Text]
Rodionov, D. G., Nordeng, T. W., Kongsvik, T. L., Bakke, O. (2000). The Cytoplasmic Tail of CD1d Contains Two Overlapping Basolateral Sorting Signals. J. Biol. Chem. 275: 8279-8282 [Abstract] [Full Text]
Hernando, N., Sheikh, S., Karim-Jimenez, Z., Galliker, H., Forgo, J., Biber, J., Murer, H. (2000). Asymmetrical targeting of type II Na-Pi cotransporters in renal and intestinal epithelial cell lines. Am. J. Physiol. Renal Physiol. 278: F361-F368 [Abstract] [Full Text]
Jacob, R., Alfalah, M., Grunberg, J., Obendorf, M., Naim, H. Y. (2000). Structural Determinants Required for Apical Sorting of an Intestinal Brush-border Membrane Protein. J. Biol. Chem. 275: 6566-6572 [Abstract] [Full Text]
Lipardi, C., Nitsch, L., Zurzolo, C. (2000). Detergent-insoluble GPI-anchored Proteins Are Apically Sorted in Fischer Rat Thyroid Cells, but Interference with Cholesterol or Sphingolipids Differentially Affects Detergent Insolubility and Apical Sorting. Mol. Biol. Cell 11: 531-542 [Abstract] [Full Text]
Marchand, S., Bignami, F., Stetzkowski-Marden, F., Cartaud, J. (2000). The Myristoylated Protein Rapsyn is Cotargeted with the Nicotinic Acetylcholine Receptor to the Postsynaptic Membrane via the Exocytic Pathway. J. Neurosci. 20: 521-528 [Abstract] [Full Text]
Luo, C., Nobusawa, E., Nakajima, K. (1999). An analysis of the role of neuraminidase in the receptor-binding activity of influenza B virus: the inhibitory effect of Zanamivir on haemadsorption. J. Gen. Virol. 80: 2969-2976 [Abstract] [Full Text]
Simonsen, A., Pedersen, K. W., Nordeng, T. W., von der Lippe, A., Stang, E., Long, E. O., Bakke, O. (1999). Polarized Transport of MHC Class II Molecules in Madin-Darby Canine Kidney Cells Is Directed by a Leucine-Based Signal in the Cytoplasmic Tail of the {beta}-Chain. J. Immunol. 163: 2540-2548 [Abstract] [Full Text]
SIMONS, M., SAFFRICH, R., REISER, J., MUNDEL, P. (1999). Directed Membrane Transport Is Involved in Process Formation in Cultured Podocytes. J. Am. Soc. Nephrol. 10: 1633-1639 [Abstract] [Full Text]
Allan, B. B., Balch, W. E. (1999). Protein Sorting by Directed Maturation of Golgi Compartments. Science 285: 63-66 [Abstract] [Full Text]
Cheong, K. H., Zacchetti, D., Schneeberger, E. E., Simons, K. (1999). VIP17/MAL, a lipid raft-associated protein, is involved in apical transport in MDCK cells. Proc. Natl. Acad. Sci. USA 96: 6241-6248 [Abstract] [Full Text]
Kachinsky, A. M., Froehner, S. C., Milgram, S. L. (1999). A PDZ-containing Scaffold Related to the Dystrophin Complex at the Basolateral Membrane of Epithelial Cells. JCB 145: 391-402 [Abstract] [Full Text]
Jacob, R., Preuss, U., Panzer, P., Alfalah, M., Quack, S., Roth, M. G., Naim, H., Naim, H. Y. (1999). Hierarchy of Sorting Signals in Chimeras of Intestinal Lactase-Phlorizin Hydrolase and the Influenza Virus Hemagglutinin. J. Biol. Chem. 274: 8061-8067 [Abstract] [Full Text]
Rodionov, D. G., Nordeng, T. W., Pedersen, K., Balk, S. P., Bakke, O. (1999). A Critical Tyrosine Residue in the Cytoplasmic Tail Is Important for CD1d Internalization But Not for Its Basolateral Sorting in MDCK Cells. J. Immunol. 162: 1488-1495 [Abstract] [Full Text]
Zheng, X., Lu, D., Sadler, J. E. (1999). Apical Sorting of Bovine Enteropeptidase Does Not Involve Detergent-resistant Association with Sphingolipid-Cholesterol Rafts. J. Biol. Chem. 274: 1596-1605 [Abstract] [Full Text]
Fullekrug, J, Scheiffele, P, Simons, K (1999). VIP36 localisation to the early secretory pathway. J. Cell Sci. 112: 2813-2821 [Abstract]
Kjer-Nielsen, L, van Vliet, C, Erlich, R, Toh, B., Gleeson, P. (1999). The Golgi-targeting sequence of the peripheral membrane protein p230. J. Cell Sci. 112: 1645-1654 [Abstract]
Chen, W., Feng, Y., Chen, D., Wandinger-Ness, A. (1998). Rab11 Is Required for Trans-Golgi Network-to-Plasma Membrane Transport and a Preferential Target for GDP Dissociation Inhibitor. Mol. Biol. Cell 9: 3241-3257 [Abstract] [Full Text]
Lafont, F., Lecat, S., Verkade, P., Simons, K. (1998). Annexin XIIIb Associates with Lipid Microdomains to Function in Apical Delivery. JCB 142: 1413-1427 [Abstract] [Full Text]
Chuang, J.-Z., Sung, C.-H. (1998). The Cytoplasmic Tail of Rhodopsin Acts as a Novel Apical Sorting Signal in Polarized MDCK Cells. JCB 142: 1245-1256 [Abstract] [Full Text]
Hennig, D., Scales, S. J., Moreau, A., Murley, L. L., De Mey, J., Kreis, T. E. (1998). A Formiminotransferase Cyclodeaminase Isoform Is Localized to the Golgi Complex and Can Mediate Interaction of Trans-Golgi Network-derived Vesicles with Microtubules. J. Biol. Chem. 273: 19602-19611 [Abstract] [Full Text]
Low, S. H., Chapin, S. J., Wimmer, C., Whiteheart, S. W., Komuves, L. G., Mostov, K. E., Weimbs, T. (1998). The SNARE Machinery Is Involved in Apical Plasma Membrane Trafficking in MDCK Cells. JCB 141: 1503-1513 [Abstract] [Full Text]
Puertollano, R., Alonso, M. A. (1998). A Short Peptide Motif at the Carboxyl Terminus Is Required for Incorporation of the Integral Membrane MAL Protein to Glycolipid-enriched Membranes. J. Biol. Chem. 273: 12740-12745 [Abstract] [Full Text]
Harder, T., Scheiffele, P., Verkade, P., Simons, K. (1998). Lipid Domain Structure of the Plasma Membrane Revealed by Patching of Membrane Components. JCB 141: 929-942 [Abstract] [Full Text]
Orci, L., Perrelet, A., Rothman, J. E. (1998). Vesicles on strings: Morphological evidence for processive transport within the Golgi stack. Proc. Natl. Acad. Sci. USA 95: 2279-2283 [Abstract] [Full Text]
Grindstaff, K. K., Bacallao, R. L., Nelson, W. J. (1998). Apiconuclear Organization of Microtubules Does Not Specify Protein Delivery from the Trans-Golgi Network to Different Membrane Domains in Polarized Epithelial Cells. Mol. Biol. Cell 9: 685-699 [Abstract] [Full Text]
Scheiffele, P., Verkade, P., Fra, A.M., Virta, H., Simons, K., Ikonen, E. (1998). Caveolin-1 and -2 in the Exocytic Pathway of MDCK Cells. JCB 140: 795-806 [Abstract] [Full Text]
Lipardi, C., Mora, R., Colomer, V., Paladino, S., Nitsch, L., Rodriguez-Boulan, E., Zurzolo, C. (1998). Caveolin Transfection Results in Caveolae Formation but Not Apical Sorting of Glycosylphosphatidylinositol (GPI)-anchored Proteins in Epithelial Cells. JCB 140: 617-626 [Abstract] [Full Text]
Simon, J.-P., Shen, T.-H., Ivanov, I. E., Gravotta, D., Morimoto, T., Adesnik, M., Sabatini, D. D. (1998). Coatomer, but not P200/myosin II, is required for the in vitro formation of trans-Golgi network-derived vesicles containing the envelope glycoprotein of vesicular stomatitis�virus. Proc. Natl. Acad. Sci. USA 95: 1073-1078 [Abstract] [Full Text]
Distel, B., Bauer, U., Le Borgne, R., Hoflack, B. (1998). Basolateral Sorting of the Cation-dependent Mannose 6-Phosphate Receptor in Madin-Darby Canine Kidney Cells. IDENTIFICATION OF A BASOLATERAL DETERMINANT UNRELATED TO CLATHRIN-COATED PIT LOCALIZATION SIGNALS. J. Biol. Chem. 273: 186-193 [Abstract] [Full Text]
Yeaman, C., Gall, A. H. L., Baldwin, A. N., Monlauzeur, L., Bivic, A. L., Rodriguez-Boulan, E. (1997). The O-glycosylated Stalk Domain Is Required for Apical Sorting of Neurotrophin Receptors in Polarized MDCK Cells. JCB 139: 929-940 [Abstract] [Full Text]
Maisner, A., Zimmer, G., Liszewski, M. K., Lublin, D. M., Atkinson, J. P., Herrler, G. (1997). Membrane Cofactor Protein (CD46) Is a Basolateral Protein That Is Not Endocytosed. IMPORTANCE OF THE TETRAPEPTIDE FTSL AT THE CARBOXYL TERMINUS. J. Biol. Chem. 272: 20793-20799 [Abstract] [Full Text]
Saunders, C., Limbird, L. E. (1997). Disruption of Microtubules Reveals Two Independent Apical Targeting Mechanisms for G-protein-coupled Receptors in Polarized Renal Epithelial Cells. J. Biol. Chem. 272: 19035-19045 [Abstract] [Full Text]
Puertollano, R., Li, S., Lisanti, M. P., Alonso, M. A. (1997). Recombinant Expression of the MAL Proteolipid, a Component of Glycolipid-enriched Membrane Microdomains, Induces the Formation of Vesicular Structures in Insect Cells. J. Biol. Chem. 272: 18311-18315 [Abstract] [Full Text]
Odorizzi, G., Trowbridge, I. S. (1997). Structural Requirements for Basolateral Sorting of the Human Transferrin Receptor in the Biosynthetic and Endocytic Pathways of Madin-Darby Canine Kidney Cells. JCB 137: 1255-1264 [Abstract] [Full Text]
Kumar, A., Wetzler, E., Berger, M. (1997). Isolation and Characterization of Complement Receptor Type 1�(CR1) Storage Vesicles From Human Neutrophils Using Antibodies to the Cytoplasmic Tail of CR1. Blood 89: 4555-4565 [Abstract] [Full Text]
Odorizzi, G., Trowbridge, I. S. (1997). Structural Requirements for Major Histocompatibility Complex Class II Invariant Chain Trafficking in Polarized Madin-Darby Canine Kidney Cells. J. Biol. Chem. 272: 11757-11762 [Abstract] [Full Text]
Marzolo, M. P., Bull, P., Gonzalez, A. (1997). Apical sorting of hepatitis B surface antigen (HBsAg) is independent of N-glycosylation and glycosylphosphatidylinositol-anchored protein�segregation. Proc. Natl. Acad. Sci. USA 94: 1834-1839 [Abstract] [Full Text]
Simonsen, A, Stang, E, Bremnes, B, Roe, M, Prydz, K, Bakke, O (1997). Sorting of MHC class II molecules and the associated invariant chain (Ii) in polarized MDCK cells. J. Cell Sci. 110: 597-609 [Abstract]
Maisner, A., Liszewski, M. K., Atkinson, J. P., Schwartz-Albiez, R., Herrler, G. (1996). Two Different Cytoplasmic Tails Direct Isoforms of the Membrane Cofactor Protein (CD46) to the Basolateral Surface of Madin-Darby Canine Kidney Cells. J. Biol. Chem. 271: 18853-18858 [Abstract] [Full Text]
Pemberton, L. F., Rughetti, A., Taylor-Papadimitriou, J., Gendler, S. J. (1996). The Epithelial Mucin MUC1 Contains at Least Two Discrete Signals Specifying Membrane Localization in Cells. J. Biol. Chem. 271: 2332-2340 [Abstract] [Full Text]
Gleeson, P., Anderson, T., Stow, J., Griffiths, G, Toh, B., Matheson, F (1996). p230 is associated with vesicles budding from the trans-Golgi network. J. Cell Sci. 109: 2811-2821 [Abstract]
Mayer, A, Ivanov, I., Gravotta, D, Adesnik, M, Sabatini, D. (1996). Cell-free reconstitution of the transport of viral glycoproteins from the TGN to the basolateral plasma membrane of MDCK cells. J. Cell Sci. 109: 1667-1676 [Abstract]
Fiedler, K, Simons, K (1996). Characterization of VIP36, an animal lectin homologous to leguminous lectins. J. Cell Sci. 109: 271-276 [Abstract]
Shen, B.-Q., Widdicombe, J. H., Mrsny, R. J. (1995). Effects of Lovastatin on Trafficking of Cystic Fibrosis Transmembrane Conductance Regulator in Human Tracheal Epithelium. J. Biol. Chem. 270: 25102-25106 [Abstract] [Full Text]
Monlauzeur, L., Rajasekaran, A., Chao, M., Rodriguez-Boulan, E., Bivic, A. L. (1995). A Cytoplasmic Tyrosine Is Essential for the Basolateral Localization of Mutants of the Human Nerve Growth Factor Receptor in Madin- Darby Canine Kidney Cells. J. Biol. Chem. 270: 12219-12225 [Abstract] [Full Text]
Simon, J.-P., Ivanov, I.E., Ren, M., Zeng, J., Shopsin, B., Hersh, D., Tempst, P., Erdjument-Bromage, H., Lui, M., De Lemos-Chiarandini, C., Rosenfeld, M., Gravotta, D., Morimoto, T., Adesnik, M., Sabatini, D.D. (1995). Regulation of Post-Golgi Vesicle Production in an In Vitro System. Cold Spring Harb Symp Quant Biol 60: 179-195 [Abstract]
Mellman, I. (1995). Molecular Sorting of Membrane Proteins in Polarized and Nonpolarized Cells. Cold Spring Harb Symp Quant Biol 60: 745-752 [Abstract]
Lafont, F., Simons, K., Ikonen, E. (1995). Dissecting the Molecular Mechanisms of Polarized Membrane Traffic: Reconstitution of Three Transport Steps in Epithelial Cells Using Streptolysin-O Permeabilization. Cold Spring Harb Symp Quant Biol 60: 753-762 [Abstract]
Lutcke, A, Parton, R., Murphy, C, Olkkonen, V., Dupree, P, Valencia, A, Simons, K, Zerial, M (1994). Cloning and subcellular localization of novel rab proteins reveals polarized and cell type-specific expression. J. Cell Sci. 107: 3437-3448 [Abstract]
Wagner, M, Rajasekaran, A., Hanzel, D., Mayor, S, Rodriguez-Boulan, E (1994). Brefeldin A causes structural and functional alterations of the trans-Golgi network of MDCK cells. J. Cell Sci. 107: 933-943 [Abstract]
Deretic, D, Papermaster, D. (1993). Rab6 is associated with a compartment that transports rhodopsin from the trans-Golgi to the site of rod outer segment disk formation in frog retinal photoreceptors. J. Cell Sci. 106: 803-813 [Abstract]
Schweizer, A, Ericsson, M, Bachi, T, Griffiths, G, Hauri, H. (1993). Characterization of a novel 63 kDa membrane protein. Implications for the organization of the ER-to-Golgi pathway. J. Cell Sci. 104: 671-683 [Abstract]
Simons, K., Dupree, P., Fiedler, K., Huber, L.A., Kobayashi, T., Kurzchalia, T., Olkkonen, V., Pimplikar, S., Parton, R., Dotti, C. (1992). Biogenesis of Cell-surface Polarity in Epithelial Cells and Neurons. Cold Spring Harb Symp Quant Biol 57: 611-619 [Abstract]
Nelson, W.J., Wilson, R., Wollner, D., Mays, R., McNeill, H., Siemers, K. (1992). Regulation of Epithelial Cell Polarity: A View from the Cell Surface. Cold Spring Harb Symp Quant Biol 57: 621-630 [Abstract]