Myosin mRNA accumulation and myofibrillogenesis at the myotendinous junction of stretched muscle fibers

doi:10.1083/jcb.111.5.1885

This Article

	Full Text (PDF, 4657K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Dix, D. J.
	Articles by Eisenberg, B. R.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Dix, D. J.
	Articles by Eisenberg, B. R.


Social Bookmarking

	What's this?

ARTICLES

Myosin mRNA accumulation and myofibrillogenesis at the myotendinous junction of stretched muscle fibers

DJ Dix and BR Eisenberg
Department of Physiology and Biophysics, University of Illinois School of Medicine, Chicago 60612.

Myofiber growth and myofibril assembly at the myotendinous junction (MTJ) of stretch-hypertrophied rabbit skeletal muscle was studied by in situ hybridization, immunofluorescence, and electron microscopy. In situ hybridization identified higher levels of myosin heavy chain (MHC) mRNA at the MTJ of fibers stretched for 4 d. Electron microscopy at the MTJ of these lengthening fibers revealed a large cytoplasmic space devoid of myofibrils, but containing polysomes, sarcoplasmic reticulum and T-membranes, mitochondria, Golgi complexes, and nascent filament assemblies. Tallies from electron micrographs indicate that myofibril assembly in stretched fibers followed a set sequence of events. (a) In stretched fiber ends almost the entire sarcolemmal membrane was electron dense but only a portion had attached myofibrils. Vinculin, detected by immunofluorescence, was greatly increased at the MTJ membrane of stretched muscles. (b) Thin filaments were anchored to the sarcolemma at the electron dense sites. (c) Thick filaments associated with these thin filaments in an unregistered manner. (d) Z-bodies splice into thin filaments and subsequently thin and thick filaments fall into sarcomeric register. Thus, the MTJ is a site of mRNA accumulation which sets up regional protein synthesis and myofibril assembly. Stretched muscles also lengthen by the addition of myotubes at their ends. After 6 d of stretch these myotubes make up the majority of fibers at the muscle ends. Essentially all these myotubes repeat the developmental program of primary myotubes and express slow MHC. MHC mRNA distribution in myotubes is disorganized as is the distribution of their myofibrils.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Clanton, T. L., Levine, S. (2009). Respiratory muscle fiber remodeling in chronic hyperinflation: dysfunction or adaptation?. J. Appl. Physiol. 107: 324-335 [Abstract] [Full Text]
Shisha, T., Kiss, S., Pap, K., Simpson, H., Szoke, G. (2006). Relative ability of young and mature muscles to respond to limb lengthening. J Bone Joint Surg Br 88-B: 1666-1669 [Abstract] [Full Text]
Joya, J. E., Kee, A. J., Nair-Shalliker, V., Ghoddusi, M., Nguyen, M.-A. T., Luther, P., Hardeman, E. C. (2004). Muscle weakness in a mouse model of nemaline myopathy can be reversed with exercise and reveals a novel myofiber repair mechanism. Hum Mol Genet 13: 2633-2645 [Abstract] [Full Text]
Russell, B., Motlagh, D., Ashley, W. W. (2000). Form follows function: how muscle shape is regulated by work. J. Appl. Physiol. 88: 1127-1132 [Abstract] [Full Text]
Tidball, J. G., Spencer, M. J., Wehling, M., Lavergne, E. (1999). Nitric-oxide Synthase Is a Mechanical Signal Transducer That Modulates Talin and Vinculin Expression. J. Biol. Chem. 274: 33155-33160 [Abstract] [Full Text]
Koh, T. J, Tidball, J. G (1999). Nitric oxide synthase inhibitors reduce sarcomere addition in rat skeletal muscle. J. Physiol. 519: 189-196 [Abstract] [Full Text]
Ennion, S, Wilkes, D, Gauvry, L, Alami-Durante, H, Goldspink, G (1999). Identification and expression analysis of two developmentally regulated myosin heavy chain gene transcripts in carp (Cyprinus carpio). J. Exp. Biol. 202: 1081-1090 [Abstract]
Frenette, J., Tidball, J. G. (1998). Mechanical loading regulates expression of talin and its mRNA, which are concentrated at myotendinous junctions. Am. J. Physiol. Cell Physiol. 275: C818-C825 [Abstract] [Full Text]
Newlands, S., Levitt, L. K., Robinson, C. S., Karpf, A.B. C., Hodgson, V. R.M., Wade, R. P., Hardeman, E. C. (1998). Transcription occurs in pulses in muscle fibers. Genes Dev. 12: 2748-2758 [Abstract] [Full Text]
Collins, T., Joya, J. E., Arkell, R. M., Ferguson, V., Hardeman, E. C. (1997). Reappearance of the minor alpha -sarcomeric actins in postnatal muscle. Am. J. Physiol. Cell Physiol. 273: C1801-C1810 [Abstract] [Full Text]
Goldspink, P, Sharp, W, Russell, B (1997). Localization of cardiac (alpha)-myosin heavy chain mRNA is regulated by its 3' untranslated region via mechanical activity and translational block. J. Cell Sci. 110: 2969-2978 [Abstract]