Cloning and expression of gamma-adaptin, a component of clathrin-coated vesicles associated with the Golgi apparatus

doi:10.1083/jcb.111.6.2319

This Article

	Full Text (PDF, 1396K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Robinson, M. S.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Robinson, M. S.


Social Bookmarking

	What's this?

ARTICLES

Cloning and expression of gamma-adaptin, a component of clathrin-coated vesicles associated with the Golgi apparatus

MS Robinson
Department of Clinical Biochemistry, University of Cambridge, Addenbrooke's Hospital, England.

Adaptins are the major components of adaptors, the protein complexes that link clathrin to transmembrane proteins (e.g., receptors) in coated pits and vesicles. The plasma membrane adaptor contains an alpha- adaptin subunit and a beta-adaptin subunit, while the Golgi adaptor contains a gamma-adaptin subunit and a beta'-adaptin subunit. A partial cDNA clone encoding gamma-adaptin was isolated from a bovine brain expression library by screening with antibodies, and was used to obtain a cDNA clone from a mouse brain library containing the full coding sequence. The identity of the clones was confirmed by protein sequencing. The deduced amino acid sequence of gamma-adaptin was found to be homologous to that of alpha-adaptin, with several stretches of identical amino acids or conservative substitutions in the first approximately 70 kD, and 25% identity overall. Weaker homology was seen between gamma- and beta-adaptins. Like both alpha- and beta-adaptins, gamma-adaptin has a proline and glycine-rich hinge region, dividing it into NH2- and COOH-terminal domains. A chimeric gamma-adaptin was constructed from the mouse and bovine cDNAs and transfected into Rat 1 fibroblasts. Immunofluorescence microscopy was carried out using an mAb which recognizes an epitope present on the chimera but not found on the rodent protein. The construct was found to have a distribution typical of endogenous gamma-adaptin. Using this transfection system, it should now be possible to exchange domains between alpha- and gamma-adaptins, to try to find out how adaptors are targeted to the appropriate membrane compartment of the cell, and how they recruit the appropriate receptors into the coated vesicle.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Fanjul, M., Alvarez, L., Hollande, E. (2007). Expression and Subcellular Localization of a 35-kDa Carbonic Anhydrase IV in a Human Pancreatic Ductal Cell Line (Capan-1). J. Histochem. Cytochem. 55: 783-794 [Abstract] [Full Text]
Nie, Z., Fei, J., Premont, R. T., Randazzo, P. A. (2005). The Arf GAPs AGAP1 and AGAP2 distinguish between the adaptor protein complexes AP-1 and AP-3. J. Cell Sci. 118: 3555-3566 [Abstract] [Full Text]
Mattera, R., Ritter, B., Sidhu, S. S., McPherson, P. S., Bonifacino, J. S. (2004). Definition of the Consensus Motif Recognized by {gamma}-Adaptin Ear Domains. J. Biol. Chem. 279: 8018-8028 [Abstract] [Full Text]
Gazina, E. V., Mackenzie, J. M., Gorrell, R. J., Anderson, D. A. (2002). Differential Requirements for COPI Coats in Formation of Replication Complexes among Three Genera of Picornaviridae. J. Virol. 76: 11113-11122 [Abstract] [Full Text]
Wedlich-Soldner, R., Schulz, I., Straube, A., Steinberg, G. (2002). Dynein Supports Motility of Endoplasmic Reticulum in the Fungus Ustilago maydis. Mol. Biol. Cell 13: 965-977 [Abstract] [Full Text]
Rous, B. A., Reaves, B. J., Ihrke, G., Briggs, J. A.G., Gray, S. R., Stephens, D. J., Banting, G., Luzio, J. P. (2002). Role of Adaptor Complex AP-3 in Targeting Wild-Type and Mutated CD63 to Lysosomes. Mol. Biol. Cell 13: 1071-1082 [Abstract] [Full Text]
Doray, B., Kornfeld, S. (2001). {gamma}Subunit of the AP-1 Adaptor Complex Binds Clathrin: Implications for Cooperative Binding in Coated Vesicle Assembly. Mol. Biol. Cell 12: 1925-1935 [Abstract] [Full Text]
Puertollano, R., Martinez-Menarguez, J. A., Batista, A., Ballesta, J., Alonso, M. A. (2001). An Intact Dilysine-like Motif in the Carboxyl Terminus of MAL Is Required for Normal Apical Transport of the Influenza Virus Hemagglutinin Cargo Protein in Epithelial Madin-Darby Canine Kidney Cells. Mol. Biol. Cell 12: 1869-1883 [Abstract] [Full Text]
Harley, C. A., Dasgupta, A., Wilson, D. W. (2001). Characterization of Herpes Simplex Virus-Containing Organelles by Subcellular Fractionation: Role for Organelle Acidification in Assembly of Infectious Particles. J. Virol. 75: 1236-1251 [Abstract] [Full Text]
Orzech, E., Cohen, S., Weiss, A., Aroeti, B. (2000). Interactions between the Exocytic and Endocytic Pathways in Polarized Madin-Darby Canine Kidney Cells. J. Biol. Chem. 275: 15207-15219 [Abstract] [Full Text]
Domin, J., Gaidarov, I., Smith, M. E. K., Keen, J. H., Waterfield, M. D. (2000). The Class II Phosphoinositide 3-Kinase PI3K-C2alpha Is Concentrated in the Trans-Golgi Network and Present in Clathrin-coated Vesicles. J. Biol. Chem. 275: 11943-11950 [Abstract] [Full Text]
Dell'Angelica, E. C., Puertollano, R., Mullins, C., Aguilar, R. C., Vargas, J. D., Hartnell, L. M., Bonifacino, J. S. (2000). Ggas: A Family of Adp Ribosylation Factor-Binding Proteins Related to Adaptors and Associated with the Golgi Complex. JCB 149: 81-94 [Abstract] [Full Text]
Poussu, A., Lohi, O., Lehto, V.-P. (2000). Vear, a Novel Golgi-associated Protein with VHS and gamma -Adaptin "Ear" Domains. J. Biol. Chem. 275: 7176-7183 [Abstract] [Full Text]
Drake, M. T., Downs, M. A., Traub, L. M. (2000). Epsin Binds to Clathrin by Associating Directly with the Clathrin-terminal Domain. EVIDENCE FOR COOPERATIVE BINDING THROUGH TWO DISCRETE SITES. J. Biol. Chem. 275: 6479-6489 [Abstract] [Full Text]
Benmerah, A., Poupon, V., Cerf-Bensussan, N., Dautry-Varsat, A. (2000). Mapping of Eps15 Domains Involved in Its Targeting to Clathrin-coated Pits. J. Biol. Chem. 275: 3288-3295 [Abstract] [Full Text]
Lee, W., Ryu, J., Souto, R. P., Pilch, P. F., Jung, C. Y. (1999). Separation and Partial Characterization of Three Distinct Intracellular GLUT4 Compartments in Rat Adipocytes. SUBCELLULAR FRACTIONATION WITHOUT HOMOGENIZATION. J. Biol. Chem. 274: 37755-37762 [Abstract] [Full Text]
Page, L. J., Sowerby, P. J., Lui, W. W.Y., Robinson, M. S. (1999). {gamma}-Synergin: An Eh Domain-Containing Protein That Interacts with {gamma}-Adaptin. JCB 146: 993-1004 [Abstract] [Full Text]
Bogan, J. S., Lodish, H. F. (1999). Two Compartments for Insulin-Stimulated Exocytosis in 3t3-L1 Adipocytes Defined by Endogenous Acrp30 and Glut4. JCB 146: 609-620 [Abstract] [Full Text]
Hirst, J., Bright, N. A., Rous, B., Robinson, M. S. (1999). Characterization of a Fourth Adaptor-related Protein Complex. Mol. Biol. Cell 10: 2787-2802 [Abstract] [Full Text]
Zizioli, D., Meyer, C., Guhde, G., Saftig, P., von Figura, K., Schu, P. (1999). Early Embryonic Death of Mice Deficient in gamma -Adaptin. J. Biol. Chem. 274: 5385-5390 [Abstract] [Full Text]
Benmerah, A, Bayrou, M, Cerf-Bensussan, N, Dautry-Varsat, A (1999). Inhibition of clathrin-coated pit assembly by an Eps15 mutant. J. Cell Sci. 112: 1303-1311 [Abstract]
Takatsu, H., Sakurai, M., Shin, H.-W., Murakami, K., Nakayama, K. (1998). Identification and Characterization of Novel Clathrin Adaptor-related Proteins. J. Biol. Chem. 273: 24693-24700 [Abstract] [Full Text]
Tai, A. W., Chuang, J.-Z., Sung, C.-H. (1998). Localization of Tctex-1, a Cytoplasmic Dynein Light Chain, to the Golgi Apparatus and Evidence for Dynein Complex Heterogeneity. J. Biol. Chem. 273: 19639-19649 [Abstract] [Full Text]
Futter, C.E., Gibson, A., Allchin, E.H., Maxwell, S., Ruddock, L.J., Odorizzi, G., Domingo, D., Trowbridge, I.S., Hopkins, C.R. (1998). In Polarized MDCK Cells Basolateral Vesicles Arise from Clathrin-{gamma}-adaptin-coated Domains on Endosomal Tubules. JCB 141: 611-623 [Abstract] [Full Text]
Kamimoto, T., Nagai, Y., Onogi, H., Muro, Y., Wakabayashi, T., Hagiwara, M. (1998). Dymple, a Novel Dynamin-like High Molecular Weight GTPase Lacking a Proline-rich Carboxyl-terminal Domain in Mammalian Cells. J. Biol. Chem. 273: 1044-1051 [Abstract] [Full Text]
Suzuki, T., Ikeda, H. (1998). The Mouse Homolog of the Bovine Leukemia Virus Receptor Is Closely Related to the delta �Subunit of Adaptor-Related Protein Complex AP-3, Not Associated with the Cell Surface. J. Virol. 72: 593-599 [Abstract] [Full Text]
Aguilar, R. C., Ohno, H., Roche, K. W., Bonifacino, J. S. (1997). Functional Domain Mapping of the Clathrin-associated Adaptor Medium Chains �1 and �2. J. Biol. Chem. 272: 27160-27166 [Abstract] [Full Text]
Simpson, F., Peden, A. A., Christopoulou, L., Robinson, M. S. (1997). Characterization of the Adaptor-related Protein Complex, AP-3. JCB 137: 835-845 [Abstract] [Full Text]
Ohno, H., Fournier, M.-C., Poy, G., Bonifacino, J. S. (1996). Structural Determinants of Interaction of Tyrosine-based Sorting Signals with the Adaptor Medium Chains. J. Biol. Chem. 271: 29009-29015 [Abstract] [Full Text]
Benmerah, A., B�gue, B., Dautry-Varsat, A., Cerf-Bensussan, N. (1996). The Ear of alpha-Adaptin Interacts with the COOH-terminal Domain of the Eps15 Protein. J. Biol. Chem. 271: 12111-12116 [Abstract] [Full Text]
Le Borgne, R., Griffiths, G., Hoflack, B. (1996). Mannose 6-Phosphate Receptors and ADP-ribosylation Factors Cooperate for High Affinity Interaction of the AP-1 Golgi Assembly Proteins with Membranes. J. Biol. Chem. 271: 2162-2170 [Abstract] [Full Text]
Ye, W., Lafer, E. M. (1995). Clathrin Binding and Assembly Activities of Expressed Domains of the Synapse-specific Clathrin Assembly Protein AP-3. J. Biol. Chem. 270: 10933-10939 [Abstract] [Full Text]
Traub, L. M., Kornfeld, S., Ungewickell, E. (1995). Different Domains of the AP-1 Adaptor Complex Are Required for Golgi Membrane Binding and Clathrin Recruitment. J. Biol. Chem. 270: 4933-4942 [Abstract] [Full Text]
Sorkin, A., McKinsey, T., Shih, W., Kirchhausen, T., Carpenter, G. (1995). Stoichiometric Interaction of the Epidermal Growth Factor Receptor with the Clathrin-associated Protein Complex AP-2. J. Biol. Chem. 270: 619-625 [Abstract] [Full Text]
Ball, C., Hunt, S., Robinson, M. (1995). Expression and localization of alpha-adaptin isoforms. J. Cell Sci. 108: 2865-2875 [Abstract]
Rad, M., Phan, H., Kirchrath, L, Tan, P., Kirchhausen, T, Hollenberg, C., Payne, G. (1995). Saccharomyces cerevisiae Apl2p, a homologue of the mammalian clathrin AP beta subunit, plays a role in clathrin-dependent Golgi functions. J. Cell Sci. 108: 1605-1615 [Abstract]
Holstein, S., Drucker, M, Robinson, D. (1994). Identification of a beta-type adaptin in plant clathrin-coated vesicles. J. Cell Sci. 107: 945-953 [Abstract]
Camidge, D., Pearse, B. (1994). Cloning of Drosophila beta-adaptin and its localization on expression in mammalian cells. J. Cell Sci. 107: 709-718 [Abstract]