BIK1, a protein required for microtubule function during mating and mitosis in Saccharomyces cerevisiae, colocalizes with tubulin

doi:10.1083/jcb.111.6.2573

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BIK1, a protein required for microtubule function during mating and mitosis in Saccharomyces cerevisiae, colocalizes with tubulin

V Berlin, CA Styles and GR Fink
Whitehead Institute for Biomedical Research, Cambridge, Massachusetts.

BIK1 function is required for nuclear fusion, chromosome disjunction, and nuclear segregation during mitosis. The BIK1 protein colocalizes with tubulin to the spindle pole body and mitotic spindle. Synthetic lethality observed in double mutant strains containing a mutation in the BIK1 gene and in the gene for alpha- or beta-tubulin is consistent with a physical interaction between BIK1 and tubulin. Furthermore, over- or underexpression of BIK1 causes aberrant microtubule assembly and function, bik1 null mutants are viable but contain very short or undetectable cytoplasmic microtubules. Spindle formation often occurs strictly within the mother cell, probably accounting for the many multinucleate and anucleate bik1 cells. Elevated levels of chromosome loss in bik1 cells are indicative of defective spindle function. Nuclear fusion is blocked in bik1 x bik1 zygotes, which have truncated cytoplasmic microtubules. Cells overexpressing BIK1 initially have abnormally short or nonexistent spindle microtubules and long cytoplasmic microtubules. Subsequently, cells lose all microtubule structures, coincident with the arrest of division. Based on these results, we propose that BIK1 is required stoichiometrically for the formation or stabilization of microtubules during mitosis and for spindle pole body fusion during conjugation.
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Yamamoto, A., Hiraoka, Y. (2003). Cytoplasmic dynein in fungi: insights from nuclear migration. J. Cell Sci. 116: 4501-4512 [Abstract] [Full Text]
Trelles-Sticken, E., Loidl, J., Scherthan, H. (2003). Increased ploidy and KAR3 and SIR3 disruption alter the dynamics of meiotic chromosomes and telomeres. J. Cell Sci. 116: 2431-2442 [Abstract] [Full Text]
van Breugel, M., Drechsel, D., Hyman, A. (2003). Stu2p, the budding yeast member of the conserved Dis1/XMAP215 family of microtubule-associated proteins is a plus end-binding microtubule destabilizer. JCB 161: 359-369 [Abstract] [Full Text]
Hwang, H.-S., Song, K. (2002). IBD2 Encodes a Novel Component of the Bub2p-Dependent Spindle Checkpoint in the Budding Yeast Saccharomyces cerevisiae. Genetics 161: 595-609 [Abstract] [Full Text]
Coquelle, F. M., Caspi, M., Cordelieres, F. P., Dompierre, J. P., Dujardin, D. L., Koifman, C., Martin, P., Hoogenraad, C. C., Akhmanova, A., Galjart, N., De Mey, J. R., Reiner, O. (2002). LIS1, CLIP-170's Key to the Dynein/Dynactin Pathway. Mol. Cell. Biol. 22: 3089-3102 [Abstract] [Full Text]
Schuyler, S., Pellman, D (2001). Search, capture and signal: games microtubules and centrosomes play. J. Cell Sci. 114: 247-255 [Abstract]
Miller, R. K., Cheng, S.-C., Rose, M. D. (2000). Bim1p/Yeb1p Mediates the Kar9p-dependent Cortical Attachment of Cytoplasmic Microtubules. Mol. Biol. Cell 11: 2949-2959 [Abstract] [Full Text]
Korinek, W. S., Copeland, M. J., Chaudhuri, A., Chant, J. (2000). Molecular Linkage Underlying Microtubule Orientation Toward Cortical Sites in Yeast. Science 287: 2257-2259 [Abstract] [Full Text]
Cullen, C. F., Deak, P., Glover, D. M., Ohkura, H. (1999). mini spindles: A Gene Encoding a Conserved Microtubule-Associated Protein Required for the Integrity of the Mitotic Spindle in Drosophila. JCB 146: 1005-1018 [Abstract] [Full Text]
Tirnauer, J. S., O'Toole, E., Berrueta, L., Bierer, B. E., Pellman, D. (1999). Yeast Bim1p Promotes the G1-specific Dynamics of Microtubules. JCB 145: 993-1007 [Abstract] [Full Text]
Diamantopoulos, G. S., Perez, F., Goodson, H. V., Batelier, G., Melki, R., Kreis, T. E., Rickard, J. E. (1999). Dynamic Localization of CLIP-170 to Microtubule Plus Ends Is Coupled to Microtubule Assembly. JCB 144: 99-112 [Abstract] [Full Text]
Feierbach, B., Nogales, E., Downing, K. H., Stearns, T. (1999). Alf1p, a CLIP-170 Domain-containing Protein, Is Functionally and Physically Associated with {alpha}-Tubulin. JCB 144: 113-124 [Abstract] [Full Text]
Hofmann, C., Cheeseman, I. M., Goode, B. L., McDonald, K. L., Barnes, G., Drubin, D. G. (1998). Saccharomyces cerevisiae Duo1p and Dam1p, Novel Proteins Involved in Mitotic Spindle Function. JCB 143: 1029-1040 [Abstract] [Full Text]
Oka, M., Nakai, M., Endo, T., Lim, C. R., Kimata, Y., Kohno, K. (1998). Loss of Hsp70-Hsp40 Chaperone Activity Causes Abnormal Nuclear Distribution and Aberrant Microtubule Formation in M-phase of Saccharomyces cerevisiae. J. Biol. Chem. 273: 29727-29737 [Abstract] [Full Text]
Miller, R. K., Heller, K. K., Frisèn, L., Wallack, D. L., Loayza, D., Gammie, A. E., Rose, M. D. (1998). The Kinesin-related Proteins, Kip2p and Kip3p, Function Differently in Nuclear Migration in Yeast. Mol. Biol. Cell 9: 2051-2068 [Abstract] [Full Text]
Chen, X. P., Yin, H., Huffaker, T. C. (1998). The Yeast Spindle Pole Body Component Spc72p Interacts with Stu2p and Is Required for Proper Microtubule Assembly. JCB 141: 1169-1179 [Abstract] [Full Text]
Dujardin, D., Wacker, U. I., Moreau, A., Schroer, T. A., Rickard, J. E., De Mey, J. R. (1998). Evidence for a Role of CLIP-170 in the Establishment of Metaphase Chromosome Alignment. JCB 141: 849-862 [Abstract] [Full Text]
Lantz, V. A., Miller, K. G. (1998). A Class VI Unconventional Myosin Is Associated with a Homologue of a Microtubule-binding Protein, Cytoplasmic Linker Protein-170, in Neurons and at the Posterior Pole of Drosophila Embryos. JCB 140: 897-910 [Abstract] [Full Text]
Miller, R. K., Rose, M. D. (1998). Kar9p Is a Novel Cortical Protein Required for Cytoplasmic Microtubule Orientation in Yeast. JCB 140: 377-390 [Abstract] [Full Text]
Beh, C. T., Brizzio, V., Rose, M. D. (1997). KAR5 Encodes a Novel Pheromone-inducible Protein Required for Homotypic Nuclear Fusion. JCB 139: 1063-1076 [Abstract] [Full Text]
Schwartz, K., Richards, K., Botstein, D. (1997). BIM1 Encodes a Microtubule-binding Protein in Yeast. Mol. Biol. Cell 8: 2677-2691 [Abstract] [Full Text]
Waterman-Storer, C. M., Holzbaur, E. L. F. (1996). The Product of the Drosophila Gene, Glued, Is the Functional Homologue of the p150[IMAGE]Component of the Vertebrate Dynactin Complex. J. Biol. Chem. 271: 1153-1159 [Abstract] [Full Text]
Guenette, S, Magendantz, M, Solomon, F (1995). Suppression of a conditional mutation in alpha-tubulin by overexpression of two checkpoint genes. J. Cell Sci. 108: 1195-1204 [Abstract]
Pierre, P., Pepperkok, R., Kreis, T. E. (1994). Molecular characterization of two functional domains of CLIP-170 in vivo. J. Cell Sci. 107: 1909-1920 [Abstract]
Aist, J., Liang, H, Berns, M. (1993). Astral and spindle forces in PtK2 cells during anaphase B: a laser microbeam study. J. Cell Sci. 104: 1207-1216 [Abstract]
Page, B D, Snyder, M (1992). CIK1: a developmentally regulated spindle pole body-associated protein important for microtubule functions in Saccharomyces cerevisiae.. Genes Dev. 6: 1414-1429 [Abstract]
Lin, H., de Carvalho, P., Kho, D., Tai, C.-Y., Pierre, P., Fink, G. R., Pellman, D. (2001). Polyploids require Bik1 for kinetochore-microtubule attachment. JCB 155: 1173-1184 [Abstract] [Full Text]