Guanine nucleotides modulate the effects of brefeldin A in semipermeable cells: regulation of the association of a 110-kD peripheral membrane protein with the Golgi apparatus

doi:10.1083/jcb.112.4.579

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Guanine nucleotides modulate the effects of brefeldin A in semipermeable cells: regulation of the association of a 110-kD peripheral membrane protein with the Golgi apparatus

JG Donaldson, J Lippincott-Schwartz and RD Klausner
Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892.

The release of a 110-kD peripheral membrane protein from the Golgi apparatus is an early event in brefeldin A (BFA) action, preceding the movement of Golgi membrane into the ER. ATP depletion also causes the reversible redistribution of the 110-kD protein from Golgi membrane into the cytosol, although no Golgi disassembly occurs. To further define the effects of BFA on the association of the 110-kD protein with the Golgi apparatus we have used filter perforation techniques to produce semipermeable cells. All previously observed effects of BFA, including the rapid redistribution of the 110-kD protein and the movement of Golgi membrane into the ER, could be reproduced in the semipermeable cells. The role of guanine nucleotides in this process was investigated using the nonhydrolyzable analogue of GTP, GTP gamma S. Pretreatment of semipermeable cells with GTP gamma S prevented the BFA-induced redistribution of the 110-kD protein from the Golgi apparatus and movement of Golgi membrane into the ER. GTP gamma S could also abrogate the observed release of the 110-kD protein from Golgi membranes which occurred in response to ATP depletion. Additionally, when the 110-kD protein had first been dissociated from Golgi membranes by ATP depletion, GTP gamma S could restore Golgi membrane association of the 110-kD protein, but not if BFA was present. All of these effects observed with GTP gamma S in semipermeable cells could be reproduced in intact cells treated with AlF4-. These results suggest that guanine nucleotides regulate the dynamic association/dissociation of the 110-kD protein with the Golgi apparatus and that BFA perturbs this process by interfering with the association of the 110-kD protein with the Golgi apparatus.
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Gazina, E. V., Mackenzie, J. M., Gorrell, R. J., Anderson, D. A. (2002). Differential Requirements for COPI Coats in Formation of Replication Complexes among Three Genera of Picornaviridae. J. Virol. 76: 11113-11122 [Abstract] [Full Text]
Dahm, T., White, J., Grill, S., Füllekrug, J., Stelzer, E. H.K. (2001). Quantitative ER {left-right-arrow} Golgi Transport Kinetics and Protein Separation upon Golgi Exit Revealed by Vesicular Integral Membrane Protein 36 Dynamics in Live Cells. Mol. Biol. Cell 12: 1481-1498 [Abstract] [Full Text]
Kegel, K. B., Kim, M., Sapp, E., McIntyre, C., Castano, J. G., Aronin, N., DiFiglia, M. (2000). Huntingtin Expression Stimulates Endosomal-Lysosomal Activity, Endosome Tubulation, and Autophagy. J. Neurosci. 20: 7268-7278 [Abstract] [Full Text]
Kano, F., Sako, Y., Tagaya, M., Yanagida, T., Murata, M. (2000). Reconstitution of Brefeldin A-induced Golgi Tubulation and Fusion with the Endoplasmic Reticulum in Semi-Intact Chinese Hamster Ovary Cells. Mol. Biol. Cell 11: 3073-3087 [Abstract] [Full Text]
Robertson, A. M., Allan, V. J. (2000). Brefeldin A-dependent Membrane Tubule Formation Reconstituted In Vitro Is Driven by a Cell Cycle-regulated Microtubule Motor. Mol. Biol. Cell 11: 941-955 [Abstract] [Full Text]
Alvarez, C., Fujita, H., Hubbard, A., Sztul, E. (1999). ER to Golgi Transport: Requirement for P115 at a Pre-Golgi Vtc Stage. JCB 147: 1205-1222 [Abstract] [Full Text]
Hu, T., Kao, C.-Y., Hudson, R. T., Chen, A., Draper, R. K. (1999). Inhibition of Secretion by 1,3-Cyclohexanebis(methylamine), a Dibasic Compound That Interferes with Coatomer Function. Mol. Biol. Cell 10: 921-933 [Abstract] [Full Text]
Blum, R, Pfeiffer, F, Feick, P, Nastainczyk, W, Kohler, B, Schafer, K., Schulz, I (1999). Intracellular localization and in vivo trafficking of p24A and p23. J. Cell Sci. 112: 537-548 [Abstract]
Zhang, Q., Cox, D., Tseng, C.-C., Donaldson, J. G., Greenberg, S. (1998). A Requirement for ARF6 in Fcgamma Receptor-mediated Phagocytosis in Macrophages. J. Biol. Chem. 273: 19977-19981 [Abstract] [Full Text]
Cole, N. B., Ellenberg, J., Song, J., DiEuliis, D., Lippincott-Schwartz, J. (1998). Retrograde Transport of Golgi-localized Proteins to the ER. JCB 140: 1-15 [Abstract] [Full Text]
Monier, S, Chardin, P, Robineau, S, Goud, B (1998). Overexpression of the ARF1 exchange factor ARNO inhibits the early secretory pathway and causes the disassembly of the Golgi complex. J. Cell Sci. 111: 3427-3436 [Abstract]
Song, J, Khachikian, Z, Radhakrishna, H, Donaldson, J. (1998). Localization of endogenous ARF6 to sites of cortical actin rearrangement and involvement of ARF6 in cell spreading. J. Cell Sci. 111: 2257-2267 [Abstract]
Kappeler, F., Klopfenstein, D. R.�Ch., Foguet, M., Paccaud, J.-P., Hauri, H.-P. (1997). The Recycling of ERGIC-53 in the Early Secretory Pathway. ERGIC-53 CARRIES A CYTOSOLIC ENDOPLASMIC RETICULUM-EXIT DETERMINANT INTERACTING WITH COPII. J. Biol. Chem. 272: 31801-31808 [Abstract] [Full Text]
Phung, T. L., Roncone, A., Jensen, K. L.�d. M., Sparks, C. E., Sparks, J. D. (1997). Phosphoinositide 3-Kinase Activity Is Necessary for Insulin-dependent Inhibition of Apolipoprotein B Secretion by Rat Hepatocytes and Localizes to the Endoplasmic Reticulum. J. Biol. Chem. 272: 30693-30702 [Abstract] [Full Text]
Sciaky, N., Presley, J., Smith, C., Zaal, K. J.M., Cole, N., Moreira, J. E., Terasaki, M., Siggia, E., Lippincott-Schwartz, J. (1997). Golgi Tubule Traffic and the Effects of Brefeldin A Visualized in Living Cells. JCB 139: 1137-1155 [Abstract] [Full Text]
Mironov, A., Colanzi, A., Silletta, M. G., Fiucci, G., Flati, S., Fusella, A., Polishchuk, R., Mironov, A. Jr., Tullio, G. D., Weigert, R., Malhotra, V., Corda, D., Matteis, M. A. D., Luini, A. (1997). Role of NAD+ and ADP-Ribosylation in the Maintenance of the Golgi Structure. JCB 139: 1109-1118 [Abstract] [Full Text]
Radhakrishna, H., Donaldson, J. G. (1997). ADP-Ribosylation Factor 6 Regulates a Novel Plasma Membrane Recycling Pathway. JCB 139: 49-61 [Abstract] [Full Text]
Weigert, R., Colanzi, A., Mironov, A., Buccione, R., Cericola, C., Sciulli, M. G., Santini, G., Flati, S., Fusella, A., Donaldson, J. G., Di Girolamo, M., Corda, D., De Matteis, M. A., Luini, A. (1997). Characterization of Chemical Inhibitors of Brefeldin A-activated Mono-ADP-ribosylation. J. Biol. Chem. 272: 14200-14207 [Abstract] [Full Text]
Scheel, J., Pepperkok, R., Lowe, M., Griffiths, G., Kreis, T. E. (1997). Dissociation of Coatomer from Membranes Is Required for Brefeldin A-induced Transfer of Golgi Enzymes to the Endoplasmic Reticulum. JCB 137: 319-333 [Abstract] [Full Text]
Liang, J. O., Kornfeld, S. (1997). Comparative Activity of ADP-ribosylation Factor Family Members in the Early Steps of Coated Vesicle Formation on Rat Liver Golgi Membranes. J. Biol. Chem. 272: 4141-4148 [Abstract] [Full Text]
Ikonen, E, de Almeid, J., Fath, K., Burgess, D., Ashman, K, Simons, K, Stow, J. (1997). Myosin II is associated with Golgi membranes: identification of p200 as nonmuscle myosin II on Golgi-derived vesicles. J. Cell Sci. 110: 2155-2164 [Abstract]
Erickson, J. W., Zhang, C.-j., Kahn, R. A., Evans, T., Cerione, R. A. (1996). Mammalian Cdc42 Is a Brefeldin A-sensitive Component of the Golgi Apparatus. J. Biol. Chem. 271: 26850-26854 [Abstract] [Full Text]
Randazzo, P. A., Terui, T., Sturch, S., Fales, H. M., Ferrige, A. G., Kahn, R. A. (1995). The Myristoylated Amino Terminus of ADP-ribosylation Factor 1 Is a Phospholipid- and GTP-sensitive Switch. J. Biol. Chem. 270: 14809-14815 [Abstract] [Full Text]
Oda, T., Chen, C.-H., Wu, H. C. (1995). Ceramide Reverses Brefeldin A (BFA) Resistance in BFA-resistant Cell Lines. J. Biol. Chem. 270: 4088-4092 [Abstract] [Full Text]
Sariola, M, Saraste, J, Kuismanen, E (1995). Communication of post-Golgi elements with early endocytic pathway: regulation of endoproteolytic cleavage of Semliki Forest virus p62 precursor. J. Cell Sci. 108: 2465-2475 [Abstract]
Hidalgo, J, Muniz, M, Velasco, A (1995). Trimeric G proteins regulate the cytosol-induced redistribution of Golgi enzymes into the endoplasmic reticulum. J. Cell Sci. 108: 1805-1815 [Abstract]
Donaldson, J.G., Radhakrishna, H., Peters, P.J. (1995). The ARF GTPases: Defining Roles in Membrane Traffic and Organelle Structure. Cold Spring Harb Symp Quant Biol 60: 229-234 [Abstract]
Montmayeur, J., Borrelli, E (1994). Targeting of G alpha i2 to the Golgi by alternative spliced carboxyl-terminal region. Science 263: 95-98 [Abstract]
Wagner, M, Rajasekaran, A., Hanzel, D., Mayor, S, Rodriguez-Boulan, E (1994). Brefeldin A causes structural and functional alterations of the trans-Golgi network of MDCK cells. J. Cell Sci. 107: 933-943 [Abstract]
de Almeida, J., Doherty, J, Ausiello, D., Stow, J. (1993). Binding of the cytosolic p200 protein to Golgi membranes is regulated by heterotrimeric G proteins. J. Cell Sci. 106: 1239-1248 [Abstract]
Roa, M, Cornet, V, Yang, C., Goud, B (1993). The small GTP-binding protein rab6p is redistributed in the cytosol by brefeldin A. J. Cell Sci. 106: 789-802 [Abstract]
Colombo, M., Mayorga, L., Casey, P., Stahl, P. (1992). Evidence of a role for heterotrimeric GTP-binding proteins in endosome fusion. Science 255: 1695-1697 [Abstract]
Donaldson, J., Kahn, R., Lippincott-Schwartz, J, Klausner, R. (1991). Binding of ARF and beta-COP to Golgi membranes: possible regulation by a trimeric G protein. Science 254: 1197-1199 [Abstract]
Ahluwalia, J. P., Topp, J. D., Weirather, K., Zimmerman, M., Stamnes, M. (2001). A Role for Calcium in Stabilizing Transport Vesicle Coats. J. Biol. Chem. 276: 34148-34155 [Abstract] [Full Text]