The signal sequence interacts with the methionine-rich domain of the 54- kD protein of signal recognition particle

doi:10.1083/jcb.113.2.229

Quantitative Colocalization Analysis Software

This Article

	Full Text (PDF, 1039K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by High, S.
	Articles by Dobberstein, B.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by High, S.
	Articles by Dobberstein, B.


Social Bookmarking

	What's this?

ARTICLES

The signal sequence interacts with the methionine-rich domain of the 54- kD protein of signal recognition particle

S High and B Dobberstein
European Molecular Biology Laboratory, Heidelberg, Germany.

The signal sequence of nascent preprolactin interacts with the 54-kD protein of the signal recognition particle (SRP54). To identify the domain or site on SRP54 that interacts with the signal sequence we used a photocross-linking approach followed by limited proteolysis and immunoprecipitation using anti-peptide antibodies specific for defined regions of SRP54. We found that the previously identified methionine- rich RNA-binding domain of SRP54 (SRP54M domain) also interacts with the signal sequence. The smallest fragment that was found to be crosslinked to the signal sequence comprised the COOH-terminal 6-kD segment of the SRP54M domain. No cross-link to the putative GTP-binding domain of SRP54 (SRP54G domain) was found. Proteolytic cleavage between the SRP54M domain and SRP54G domain did not impair the subsequent interaction between the signal sequence and the SRP54M domain. Our results show that both the RNA binding and signal sequence binding functions of SRP54 are performed by the SRP54M domain.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Hainzl, T., Huang, S., Sauer-Eriksson, A. E. (2007). Interaction of signal-recognition particle 54 GTPase domain and signal-recognition particle RNA in the free signal-recognition particle. Proc. Natl. Acad. Sci. USA 104: 14911-14916 [Abstract] [Full Text]
Fehrmann, F., Jung, M., Zimmermann, R., Krausslich, H.-G. (2003). Transport of the Intracisternal A-Type Particle Gag Polyprotein to the Endoplasmic Reticulum Is Mediated by the Signal Recognition Particle. J. Virol. 77: 6293-6304 [Abstract] [Full Text]
GU, S.-Q., PESKE, F., WIEDEN, H.-J., RODNINA, M. V., WINTERMEYER, W. (2003). The signal recognition particle binds to protein L23 at the peptide exit of the Escherichia coli ribosome. RNA 9: 566-573 [Abstract] [Full Text]
Cleverley, R. M., Gierasch, L. M. (2002). Mapping the Signal Sequence-binding Site on SRP Reveals a Significant Role for the NG Domain. J. Biol. Chem. 277: 46763-46768 [Abstract] [Full Text]
Tian, H., Beckwith, J. (2002). Genetic Screen Yields Mutations in Genes Encoding All Known Components of the Escherichia coli Signal Recognition Particle Pathway. J. Bacteriol. 184: 111-118 [Abstract] [Full Text]
Bacher, G., Pool, M., Dobberstein, B. (1999). The Ribosome Regulates the Gtpase of the {beta}-Subunit of the Signal Recognition Particle Receptor. JCB 146: 723-730 [Abstract] [Full Text]
Nakamura, K., Yahagi, S.-i., Yamazaki, T., Yamane, K. (1999). Bacillus subtilis Histone-like Protein, HBsu, Is an Integral Component of a SRP-like Particle That Can Bind the Alu Domain of Small Cytoplasmic RNA. J. Biol. Chem. 274: 13569-13576 [Abstract] [Full Text]
Taylor, G. A., Jeffers, M., Largaespada, D. A., Jenkins, N. A., Copeland, N. G., Woude, G. F.V. (1996). Identification of a Novel GTPase, the Inducibly Expressed GTPase, That Accumulates in Response to Interferon gamma. J. Biol. Chem. 271: 20399-20405 [Abstract] [Full Text]
Kurita, K., Honda, K., Suzuma, S., Takamatsu, H., Nakamura, K., Yamane, K. (1996). Identification of a Region of Bacillus subtilis Ffh, a Homologue of Mammalian SRP54 Protein, That Is Essential for Binding to Small Cytoplasmic RNA. J. Biol. Chem. 271: 13140-13146 [Abstract] [Full Text]
Rapoport, T. (1992). Transport of proteins across the endoplasmic reticulum membrane. Science 258: 931-936 [Abstract]
Swain, J. F., Gierasch, L. M. (2001). Signal Peptides Bind and Aggregate RNA. AN ALTERNATIVE EXPLANATION FOR GTPase INHIBITION IN THE SIGNAL RECOGNITION PARTICLE. J. Biol. Chem. 276: 12222-12227 [Abstract] [Full Text]