pH-induced microtubule-dependent redistribution of late endosomes in neuronal and epithelial cells

doi:10.1083/jcb.113.2.261

This Article

	Full Text (PDF, 6238K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Parton, R. G.
	Articles by Prydz, K.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Parton, R. G.
	Articles by Prydz, K.


Social Bookmarking

	What's this?

ARTICLES

pH-induced microtubule-dependent redistribution of late endosomes in neuronal and epithelial cells

RG Parton, CG Dotti, R Bacallao, I Kurtz, K Simons and K Prydz
European Molecular Biology Laboratory, Cell Biology Program, Heidelberg, Federal Republic of Germany.

The interaction between late endocytic structures and microtubules in polarized cells was studied using a procedure previously shown to cause microtubule-dependent redistribution of lysosomes in fibroblasts and macrophages (Heuser, J. 1989. J. Cell Biol. 108:855-864). In cultured rat hippocampal neurons, low cytoplasmic pH caused cation-independent mannose-6-phosphate receptor-enriched structures to move out of the cell body and into the processes. In filter grown MDCK cells lowering the cytosolic pH to approximately 6.5 caused late endosomes to move to the base of the cell and this process was shown to be microtubule dependent. Alkalinization caused a shift in distribution towards the apical pole of the cell. The results are consistent with low pH causing the redistribution of late endosomes towards the plus ends of the microtubules. In MDCK cells the microtubules orientated vertically in the cell may play a role in this process. The shape changes that accompanied the redistribution of the late endosomes in MDCK cells were examined by electron microscopy. On low pH treatment fragmentation of the late endosomes was observed whereas after microtubule depolymerization individual late endosomal structures appeared to fuse together. The late endosomes of the MDCK cell appear to be highly pleomorphic and dependent on microtubules for their form and distribution in the cell.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Nabavi, N., Urukova, Y., Cardelli, M., Aubin, J. E., Harrison, R. E. (2008). Lysosome Dispersion in Osteoblasts Accommodates Enhanced Collagen Production during Differentiation. J. Biol. Chem. 283: 19678-19690 [Abstract] [Full Text]
Kim, H.-S., Takahashi, M., Matsuo, K., Ono, Y. (2007). Recruitment of CG-NAP to the Golgi apparatus through interaction with dynein-dynactin complex. GENES CELLS 12: 421-434 [Abstract] [Full Text]
Ro, H.-a., Carson, J. H. (2004). pH Microdomains in Oligodendrocytes. J. Biol. Chem. 279: 37115-37123 [Abstract] [Full Text]
TUMA, P. L., HUBBARD, A. L. (2003). Transcytosis: Crossing Cellular Barriers. Physiol. Rev. 83: 871-932 [Abstract] [Full Text]
Tapper, H., Furuya, W., Grinstein, S. (2002). Localized Exocytosis of Primary (Lysosomal) Granules During Phagocytosis: Role of Ca2+-Dependent Tyrosine Phosphorylation and Microtubules. J. Immunol. 168: 5287-5296 [Abstract] [Full Text]
Yang, X., Amemiya, M., Peng, Y., Moe, O. W., Preisig, P. A., Alpern, R. J. (2000). Acid incubation causes exocytic insertion of NHE3 in OKP cells. Am. J. Physiol. Cell Physiol. 279: C410-C419 [Abstract] [Full Text]
Henkel, J. R., Gibson, G. A., Poland, P. A., Ellis, M. A., Hughey, R. P., Weisz, O. A. (2000). Influenza M2 Proton Channel Activity Selectively Inhibits Trans-Golgi Network Release of Apical Membrane and Secreted Proteins in Polarized Madin-Darby Canine Kidney Cells. JCB 148: 495-504 [Abstract] [Full Text]
Vaughan, K., Tynan, S., Faulkner, N., Echeverri, C., Vallee, R. (1999). Colocalization of cytoplasmic dynein with dynactin and CLIP-170 at microtubule distal ends. J. Cell Sci. 112: 1437-1447 [Abstract]
Bayer, N., Schober, D., Prchla, E., Murphy, R. F., Blaas, D., Fuchs, R. (1998). Effect of Bafilomycin A1 and Nocodazole on Endocytic Transport in HeLa Cells: Implications for Viral Uncoating and Infection. J. Virol. 72: 9645-9655 [Abstract] [Full Text]
Verhey, K. J., Lizotte, D. L., Abramson, T., Barenboim, L., Schnapp, B. J., Rapoport, T. A. (1998). Light Chain- dependent Regulation of Kinesin's Interaction with Microtubules. JCB 143: 1053-1066 [Abstract] [Full Text]
Henkel, J. R., Apodaca, G., Altschuler, Y., Hardy, S., Weisz, O. A. (1998). Selective Perturbation of Apical Membrane Traffic by Expression of Influenza M2, an Acid-activated Ion Channel, in Polarized Madin-Darby Canine Kidney Cells. Mol. Biol. Cell 9: 2477-2490 [Abstract] [Full Text]
Daro, E., Sheff, D., Gomez, M., Kreis, T., Mellman, I. (1997). Inhibition of Endosome Function in CHO Cells Bearing a Temperature-sensitive Defect in the Coatomer (COPI) Component {varepsilon}-COP. JCB 139: 1747-1759 [Abstract] [Full Text]
Nabi, I. R., Guay, G., Simard, D. (1997). AMF-R Tubules Concentrate in a Pericentriolar Microtubule Domain After MSV Transformation of Epithelial MDCK Cells. J. Histochem. Cytochem. 45: 1351-1364 [Abstract] [Full Text]
Blocker, A., Severin, F. F., Burkhardt, J. K., Bingham, J. B., Yu, H., Olivo, J.-C., Schroer, T. A., Hyman, A. A., Griffiths, G. (1997). Molecular Requirements for Bi-directional Movement of Phagosomes Along Microtubules. JCB 137: 113-129 [Abstract] [Full Text]
Rajas, F., Gire, V., Rousset, B. (1996). Involvement of a Membrane-bound Form of Glutamate Dehydrogenase in the Association of Lysosomes to Microtubules. J. Biol. Chem. 271: 29882-29890 [Abstract] [Full Text]
Blocker, A., Severin, F. F., Habermann, A., Hyman, A. A., Griffiths, G., Burkhardt, J. K. (1996). Microtubule-associated Protein-dependent Binding of Phagosomes to Microtubules. J. Biol. Chem. 271: 3803-3811 [Abstract] [Full Text]
Sofer, A, Schwarzmann, G, Futerman, A. (1996). The internalization of a short acyl chain analogue of ganglioside GM1 in polarized neurons. J. Cell Sci. 109: 2111-2119 [Abstract]
van Deurs, B, von Bulow, F, Vilhardt, F, Holm, P., Sandvig, K (1996). Destabilization of plasma membrane structure by prevention of actin polymerization. Microtubule-dependent tubulation of the plasma membrane. J. Cell Sci. 109: 1655-1665 [Abstract]
Rapp, S, Saffrich, R, Anton, M, Jakle, U, Ansorge, W, Gorgas, K, Just, W. (1996). Microtubule-based peroxisome movement. J. Cell Sci. 109: 837-849 [Abstract]
Sloane, B., Moin, K, Sameni, M, Tait, L., Rozhin, J, Ziegler, G (1994). Membrane association of cathepsin B can be induced by transfection of human breast epithelial cells with c-Ha-ras oncogene. J. Cell Sci. 107: 373-384 [Abstract]
van Meer, G, van 't Hof, W (1993). Epithelial sphingolipid sorting is insensitive to reorganization of the Golgi by nocodazole, but is abolished by monensin in MDCK cells and by brefeldin A in Caco-2 cells. J. Cell Sci. 104: 833-842 [Abstract]
Parton, R. G., Schrotz, P., Bucci, C., Gruenberg, J. (1992). Plasticity of early endosomes. J. Cell Sci. 103: 335-348 [Abstract]
Bogner, P., Skehan, P., Kenney, S., Sainz, E., Akeson, M. A., Friedman, S. J. (1992). Stabilization of intercellular contacts in MDCK cells during Ca2+ deprivation. Selective effects of monocarboxylic acids on desmosomes. J. Cell Sci. 103: 463-473 [Abstract]