A complex of nuclear pore proteins required for pore function

doi:10.1083/jcb.114.1.169

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A complex of nuclear pore proteins required for pore function

DR Finlay, E Meier, P Bradley, J Horecka and DJ Forbes
Department of Biology, University of California, San Diego, La Jolla 92093.

A family of proteins bearing novel N-acetylglucosamine residues has previously been found to be required to form functional nuclear pores. To begin to determine which of the proteins in this family are essential for pore function, antisera were raised to each of three members of the family, p62, p58, and p54. With these antisera, it was possible to deplete nuclear reconstitution extracts of the proteins and to test the depleted nuclei for nuclear transport. In the course of the experiments, it was found that the three proteins exist as a complex; antisera to any one, while specific on a protein blot, coimmunoprecipitated all three proteins. This complex of pore proteins is stable to 2 M salt, 2 M urea, and the detergent Mega 10, indicating the presence of specific and tight protein-protein interactions. By gel filtration, the complex has a molecular mass of 550-600 kD. Nuclei containing pores depleted of the complex are found to be defective for nuclear transport; moreover, we observe a strict linear correlation between the amount of complex present in nuclei and the amount of nuclear transport of which those nuclei are capable. Thus, the p62-p58- p54 complex defines a group of proteins with strong protein-protein interactions that form a unit of pore structure essential for pore function.
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Bailer, S. M., Balduf, C., Hurt, E. (2001). The Nsp1p Carboxy-Terminal Domain Is Organized into Functionally Distinct Coiled-Coil Regions Required for Assembly of Nucleoporin Subcomplexes and Nucleocytoplasmic Transport. Mol. Cell. Biol. 21: 7944-7955 [Abstract] [Full Text]
Ben-Efraim, I., Gerace, L. (2001). Gradient of Increasing Affinity of Importin {beta} for Nucleoporins along the Pathway of Nuclear Import. JCB 152: 411-418 [Abstract] [Full Text]
Elton, D., Simpson-Holley, M., Archer, K., Medcalf, L., Hallam, R., McCauley, J., Digard, P. (2001). Interaction of the Influenza Virus Nucleoprotein with the Cellular CRM1-Mediated Nuclear Export Pathway. J. Virol. 75: 408-419 [Abstract] [Full Text]
Miller, B. R., Powers, M., Park, M., Fischer, W., Forbes, D. J. (2000). Identification of a New Vertebrate Nucleoporin, Nup188, with the Use of a Novel Organelle Trap Assay. Mol. Biol. Cell 11: 3381-3396 [Abstract] [Full Text]
Ho, A. K., Shen, T. X., Ryan, K. J., Kiseleva, E., Levy, M. A., Allen, T. D., Wente, S. R. (2000). Assembly and Preferential Localization of Nup116p on the Cytoplasmic Face of the Nuclear Pore Complex by Interaction with Nup82p. Mol. Cell. Biol. 20: 5736-5748 [Abstract] [Full Text]
Allen, T., Cronshaw, J., Bagley, S, Kiseleva, E, Goldberg, M. (2000). The nuclear pore complex: mediator of translocation between nucleus and cytoplasm. J. Cell Sci. 113: 1651-1659 [Abstract]
Kehlenbach, R. H., Dickmanns, A., Kehlenbach, A., Guan, T., Gerace, L. (1999). A Role for RanBP1 in the Release of CRM1 from the Nuclear Pore Complex in a Terminal Step of Nuclear Export. JCB 145: 645-657 [Abstract] [Full Text]
Bodoor, K, Shaikh, S, Salina, D, Raharjo, W., Bastos, R, Lohka, M, Burke, B (1999). Sequential recruitment of NPC proteins to the nuclear periphery at the end of mitosis. J. Cell Sci. 112: 2253-2264 [Abstract]
Belgareh, N., Snay-Hodge, C., Pasteau, F., Dagher, S., Cole, C. N., Doye, V. (1998). Functional Characterization of a Nup159p-containing Nuclear Pore Subcomplex. Mol. Biol. Cell 9: 3475-3492 [Abstract] [Full Text]
Fahrenkrog, B., Hurt, E. C., Aebi, U., Pante, N. (1998). Molecular Architecture of the Yeast Nuclear Pore Complex: Localization of Nsp1p Subcomplexes. JCB 143: 577-588 [Abstract] [Full Text]
Bucci, M., Wente, S. R. (1998). A Novel Fluorescence-based Genetic Strategy Identifies Mutants of Saccharomyces cerevisiae Defective for Nuclear Pore Complex Assembly. Mol. Biol. Cell 9: 2439-2461 [Abstract] [Full Text]
Popov, S., Rexach, M., Ratner, L., Blobel, G., Bukrinsky, M. (1998). Viral Protein R Regulates Docking of the HIV-1 Preintegration Complex to the Nuclear Pore Complex. J. Biol. Chem. 273: 13347-13352 [Abstract] [Full Text]
Shah, S., Tugendreich, S., Forbes, D. (1998). Major Binding Sites for the Nuclear Import Receptor Are the Internal Nucleoporin Nup153 and the Adjacent Nuclear Filament Protein Tpr. JCB 141: 31-49 [Abstract] [Full Text]
Lu, D., Yang, H., Raizada, M. K. (1998). Involvement of p62 Nucleoporin in Angiotensin II-Induced Nuclear Translocation of STAT3 in Brain Neurons. J. Neurosci. 18: 1329-1336 [Abstract] [Full Text]
Ho, A. K., Raczniak, G. A., Ives, E. B., Wente, S. R. (1998). The Integral Membrane Protein Snl1p Is Genetically Linked to Yeast Nuclear Pore Complex Function. Mol. Biol. Cell 9: 355-373 [Abstract] [Full Text]
Grandi, P., Dang, T., Pané, N., Shevchenko, A., Mann, M., Forbes, D., Hurt, E. (1997). Nup93, a Vertebrate Homologue of Yeast Nic96p, Forms a Complex with a Novel 205-kDa Protein and Is Required for Correct Nuclear Pore Assembly. Mol. Biol. Cell 8: 2017-2038 [Abstract] [Full Text]
Bastos, R., Pouplana, L. R. d., Enarson, M., Bodoor, K., Burke, B. (1997). Nup84, A Novel Nucleoporin That Is Associated With CAN/Nup214 on the Cytoplasmic Face of the Nuclear Pore Complex. JCB 137: 989-1000 [Abstract] [Full Text]
Bucci, M., Wente, S. R. (1997). In Vivo Dynamics of Nuclear Pore Complexes in Yeast. JCB 136: 1185-1199 [Abstract] [Full Text]
Powers, M. A., Forbes, D. J., Dahlberg, J. E., Lund, E. (1997). The Vertebrate GLFG Nucleoporin, Nup98, Is an Essential Component of Multiple RNA Export Pathways. JCB 136: 241-250 [Abstract] [Full Text]
Goldberg, M., Wiese, C, Allen, T., Wilson, K. (1997). Dimples, pores, star-rings, and thin rings on growing nuclear envelopes: evidence for structural intermediates in nuclear pore complex assembly. J. Cell Sci. 110: 409-420 [Abstract]
Allende, M L, Amsterdam, A, Becker, T, Kawakami, K, Gaiano, N, Hopkins, N (1996). Insertional mutagenesis in zebrafish identifies two novel genes, pescadillo and dead eye, essential for embryonic development.. Genes Dev. 10: 3141-3155 [Abstract]
Emig, S., Schmalz, D., Shakibaei, M., Buchner, K. (1995). The Nuclear Pore Complex Protein p62 Is One of Several Sialic Acid-containing Proteins of the Nuclear Envelope. J. Biol. Chem. 270: 13787-13793 [Abstract] [Full Text]
Macaulay, C., Meier, E., Forbes, D. J. (1995). Differential Mitotic Phosphorylation of Proteins of the Nuclear Pore Complex. J. Biol. Chem. 270: 254-262 [Abstract] [Full Text]
Fabre, E., Schlaich, N.L., Hurt, E.C. (1995). Nucleocytoplasmic Trafficking: What Role for Repeated Motifs in Nucleoporins?. Cold Spring Harb Symp Quant Biol 60: 677-685 [Abstract]
Melchior, F., Guan, T., Paschal, B., Gerace, L. (1995). Biochemical and Structural Analysis of Nuclear Protein Import. Cold Spring Harb Symp Quant Biol 60: 707-716 [Abstract]
Portoles, M, Faura, M, Renau-Piqueras, J, Iborra, F., Saez, R, Guerri, C, Serratosa, J, Rius, E, Bachs, O (1994). Nuclear calmodulin/62 kDa calmodulin-binding protein complexes in interphasic and mitotic cells. J. Cell Sci. 107: 3601-3614 [Abstract]
Buss, F, Kent, H, Stewart, M, Bailer, S., Hanover, J. (1994). Role of different domains in the self-association of rat nucleoporin p62. J. Cell Sci. 107: 631-638 [Abstract]
Dingwall, C, Laskey, R (1992). The nuclear membrane. Science 258: 942-947 [Abstract]
Bailer, S. M., Balduf, C., Katahira, J., Podtelejnikov, A., Rollenhagen, C., Mann, M., Pante, N., Hurt, E. (2000). Nup116p Associates with the Nup82p-Nsp1p-Nup159p Nucleoporin Complex. J. Biol. Chem. 275: 23540-23548 [Abstract] [Full Text]
Lai, M.-C., Lin, R.-I., Tarn, W.-Y. (2001). Transportin-SR2 mediates nuclear import of phosphorylated SR proteins. Proc. Natl. Acad. Sci. USA 98: 10154-10159 [Abstract] [Full Text]