Chemical subdomains within the kinetochore domain of isolated CHO mitotic chromosomes

doi:10.1083/jcb.114.2.285

This Article

Full Text (PDF, 1418K)

Alert me when this article is cited

Citation Map

Services

Email this article

Similar articles in this journal

Similar articles in PubMed

Alert me to new content in the JCB

Download to citation manager

Citing Articles

Citing Articles via HighWire

Citing Articles via CrossRef

Citing Articles via Google Scholar

Google Scholar

Articles by Wordeman, L.

Articles by Mitchison, T.

Search for Related Content

PubMed

PubMed Citation

Articles by Wordeman, L.

Articles by Mitchison, T.

Pubmed/NCBI databases

Substance via MeSH

Social Bookmarking

What's this?

ARTICLES

Chemical subdomains within the kinetochore domain of isolated CHO mitotic chromosomes

L Wordeman, ER Steuer, MP Sheetz and T Mitchison
Department of Pharmacology, University of California, San Francisco 94143.

We have used indirect immunofluorescence in combination with correlative EM to subdivide the mammalian kinetochore into two domains based on the localization of specific antigens. We demonstrate here that the fibrous corona on the distal face of the kinetochore plate contains tubulin (previously shown by Mitchison, T. J., and M. W. Kirschner. 1985. J. Cell Biol. 101:755-765) and the minus end-directed, ATP-dependent microtubule motor protein, dynein; whereas a 50-kD CREST antigen is located internal to these components in the kinetochore. Tubulin and dynein can be extracted from the kinetochore by 150 mM KI, leaving other, as yet uncharacterized, components of the kinetochore corona intact. Microtubules and tubulin subunits will associate with kinetochores in vitro after extraction with 150 mM KI, suggesting that other functionally significant, corona-associated molecules remain unextracted. Our results suggest that the corona region of the kinetochore contains the machinery for chromosome translocation along microtubules.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Maresca, T. J., Salmon, E. D. (2009). Intrakinetochore stretch is associated with changes in kinetochore phosphorylation and spindle assembly checkpoint activity. JCB 184: 373-381 [Abstract] [Full Text]
Whyte, J., Bader, J. R., Tauhata, S. B.F., Raycroft, M., Hornick, J., Pfister, K. K., Lane, W. S., Chan, G. K., Hinchcliffe, E. H., Vaughan, P. S., Vaughan, K. T. (2008). Phosphorylation regulates targeting of cytoplasmic dynein to kinetochores during mitosis. JCB 183: 819-834 [Abstract] [Full Text]
Maiato, H., DeLuca, J., Salmon, E. D., Earnshaw, W. C. (2004). The dynamic kinetochore-microtubule interface. J. Cell Sci. 117: 5461-5477 [Abstract] [Full Text]
McCleland, M. L., Gardner, R. D., Kallio, M. J., Daum, J. R., Gorbsky, G. J., Burke, D. J., Stukenberg, P. T. (2003). The highly conserved Ndc80 complex is required for kinetochore assembly, chromosome congression, and spindle checkpoint activity. Genes Dev. 17: 101-114 [Abstract] [Full Text]
Pluta, A., Earnshaw, W., Goldberg, I. (2000). Interphase-specific association of intrinsic centromere protein CENP-C with HDaxx, a death domain-binding protein implicated in Fas-mediated cell death. J. Cell Sci. 111: 2029-2041 [Abstract]
Starr, D., Saffery, R, Li, Z, Simpson, A., Choo, K., Yen, T., Goldberg, M. (2000). HZwint-1, a novel human kinetochore component that interacts with HZW10. J. Cell Sci. 113: 1939-1950 [Abstract]
Aagaard, L, Schmid, M, Warburton, P, Jenuwein, T (2000). Mitotic phosphorylation of SUV39H1, a novel component of active centromeres, coincides with transient accumulation at mammalian centromeres. J. Cell Sci. 113: 817-829 [Abstract]
Zhu, X. (1999). Structural Requirements and Dynamics of Mitosin-Kinetochore Interaction in M Phase. Mol. Cell. Biol. 19: 1016-1024 [Abstract] [Full Text]
Starr, D. A., Williams, B. C., Hays, T. S., Goldberg, M. L. (1998). ZW10 Helps Recruit Dynactin and Dynein to the Kinetochore. JCB 142: 763-774 [Abstract] [Full Text]
Desai, A., Deacon, H. W., Walczak, C. E., Mitchison, T. J. (1997). A method that allows the assembly of kinetochore components onto chromosomes condensed in clarified Xenopus egg�extracts. Proc. Natl. Acad. Sci. USA 94: 12378-12383 [Abstract] [Full Text]
Yao, X., Anderson, K. L., Cleveland, D. W. (1997). The Microtubule-dependent Motor Centromere-associated Protein E (CENP-E) Is an Integral Component of Kinetochore Corona Fibers That Link Centromeres to Spindle Microtubules. JCB 139: 435-447 [Abstract] [Full Text]
Pluta, A. F., Earnshaw, W. C. (1996). Specific Interaction between Human Kinetochore Protein CENP-C and a Nucleolar Transcriptional Regulator. J. Biol. Chem. 271: 18767-18774 [Abstract] [Full Text]
Waters, J., Skibbens, R., Salmon, E. (1996). Oscillating mitotic newt lung cell kinetochores are, on average, under tension and rarely push. J. Cell Sci. 109: 2823-2831 [Abstract]
Pluta, A. F., Mackay, A. M., Ainsztein, A. M., Goldberg, I. G., Earnshaw, W. C. (1995). The Centromere: Hub of Chromosomal Activities. Science 270: 1591-1594 [Abstract]
Vallee, R.B., Vaughan, K.T., Escheverri, C.J. (1995). Targeting of Cytoplasmic Dynein to Membranous Organelles and Kinetochores via Dynactin. Cold Spring Harb Symp Quant Biol 60: 803-811 [Abstract]
Asai, D., Beckwith, S., Kandl, K., Keating, H., Tjandra, H, Forney, J. (1994). The dynein genes of Paramecium tetraurelia. Sequences adjacent to the catalytic P-loop identify cytoplasmic and axonemal heavy chain isoforms. J. Cell Sci. 107: 839-847 [Abstract]