Characterization of SIS1, a Saccharomyces cerevisiae homologue of bacterial dnaJ proteins

doi:10.1083/jcb.114.4.623

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Characterization of SIS1, a Saccharomyces cerevisiae homologue of bacterial dnaJ proteins

MM Luke, A Sutton and KT Arndt
Cold Spring Harbor Laboratory, New York 11724-2212.

The Saccharomyces cerevisiae SIS1 gene was identified as a high copy number suppressor of the slow growth phenotype of strains containing mutations in the SIT4 gene, which encodes a predicted serine/threonine protein phosphatase. The SIS1 protein is similar to bacterial dnaJ proteins in the amino-terminal third and carboxyl-terminal third of the proteins. In contrast, the middle third of SIS1 is not similar to dnaJ proteins. This region of SIS1 contains a glycine/methionine-rich region which, along with more amino-terminal sequences, is required for SIS1 to associate with a protein of apparent molecular mass of 40 kD. The SIS1 gene is essential. Strains limited for the SIS1 protein accumulate cells that appear blocked for migration of the nucleus from the mother cell into the daughter cell. In addition, many of the cells become very large and contain a large vacuole. The SIS1 protein is localized throughout the cell but is more concentrated at the nucleus. About one- fourth of the SIS1 protein is released from a nuclear fraction upon treatment with RNase. We also show that overexpression of YDJ1, another yeast protein with similarity to bacterial dnaJ proteins, can not substitute for SIS1.
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Aron, R., Lopez, N., Walter, W., Craig, E. A., Johnson, J. (2005). In Vivo Bipartite Interaction Between the Hsp40 Sis1 and Hsp70 in Saccharomyces cerevisiae. Genetics 169: 1873-1882 [Abstract] [Full Text]
Fan, C.-Y., Ren, H.-Y., Lee, P., Caplan, A. J., Cyr, D. M. (2005). The Type I Hsp40 Zinc Finger-like Region Is Required for Hsp70 to Capture Non-native Polypeptides from Ydj1. J. Biol. Chem. 280: 695-702 [Abstract] [Full Text]
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Han, W., Christen, P. (2003). Mechanism of the Targeting Action of DnaJ in the DnaK Molecular Chaperone System. J. Biol. Chem. 278: 19038-19043 [Abstract] [Full Text]
Lopez, N., Aron, R., Craig, E. A. (2003). Specificity of Class II Hsp40 Sis1 in Maintenance of Yeast Prion [RNQ+]. Mol. Biol. Cell 14: 1172-1181 [Abstract] [Full Text]
Lee, S., Fan, C. Y., Younger, J. M., Ren, H., Cyr, D. M. (2002). Identification of Essential Residues in the Type II Hsp40 Sis1 That Function in Polypeptide Binding. J. Biol. Chem. 277: 21675-21682 [Abstract] [Full Text]
Shen, Y., Meunier, L., Hendershot, L. M. (2002). Identification and Characterization of a Novel Endoplasmic Reticulum (ER) DnaJ Homologue, Which Stimulates ATPase Activity of BiP in Vitro and Is Induced by ER Stress. J. Biol. Chem. 277: 15947-15956 [Abstract] [Full Text]
Salmon, D., Montero-Lomeli, M., Goldenberg, S. (2001). A DnaJ-like Protein Homologous to the Yeast Co-chaperone Sis1 (TcJ6p) Is Involved in Initiation of Translation in Trypanosoma cruzi. J. Biol. Chem. 276: 43970-43979 [Abstract] [Full Text]
Berruti, G., Martegani, E. (2001). MSJ-1, a Mouse Testis-Specific DnaJ Protein, Is Highly Expressed in Haploid Male Germ Cells and Interacts with the Testis-Specific Heat Shock Protein Hsp70-2. Biol. Reprod. 65: 488-495 [Abstract] [Full Text]
Johnson, J. L., Craig, E. A. (2001). An Essential Role for the Substrate-Binding Region of Hsp40s in Saccharomyces cerevisiae. JCB 152: 851-856 [Abstract] [Full Text]
Yan, W., Craig, E. A. (1999). The Glycine-Phenylalanine-Rich Region Determines the Specificity of the Yeast Hsp40 Sis1. Mol. Cell. Biol. 19: 7751-7758 [Abstract] [Full Text]
Merlin, A., Voos, W., Maarse, A. C., Meijer, M., Pfanner, N., Rassow, J. (1999). The J-related Segment of Tim44 Is Essential for Cell Viability: A Mutant Tim44 Remains in the Mitochondrial Import Site, but Inefficiently Recruits mtHsp70 and Impairs Protein Translocation. JCB 145: 961-972 [Abstract] [Full Text]
Lu, Z., Cyr, D. M. (1998). Protein Folding Activity of Hsp70 Is Modified Differentially by the Hsp40 Co-chaperones Sis1 and Ydj1. J. Biol. Chem. 273: 27824-27830 [Abstract] [Full Text]
Terada, K., Kanazawa, M., Bukau, B., Mori, M. (1997). The Human DnaJ Homologue dj2 Facilitates Mitochondrial Protein Import and Luciferase Refolding. JCB 139: 1089-1095 [Abstract] [Full Text]
Leung, S.-M., Hightower, L. E. (1997). A 16-kDa Protein Functions as a New Regulatory Protein for Hsc70 Molecular Chaperone and Is Identified as a Member of the Nm23/Nucleoside Diphosphate Kinase Family. J. Biol. Chem. 272: 2607-2614 [Abstract] [Full Text]
Tsai, J., Douglas, M. G. (1996). A Conserved HPD Sequence of the J-domain Is Necessary for YDJ1 Stimulation of Hsp70 ATPase Activity at a Site Distinct from Substrate Binding. J. Biol. Chem. 271: 9347-9354 [Abstract] [Full Text]
Zhong, T., Luke, M. M., Arndt, K. T. (1996). Transcriptional Regulation of the Yeast DnaJ Homologue SIS1. J. Biol. Chem. 271: 1349-1356 [Abstract] [Full Text]
Wei, J., Hendershot, L. M. (1995). Characterization of the Nucleotide Binding Properties and ATPase Activity of Recombinant Hamster BiP Purified from Bacteria. J. Biol. Chem. 270: 26670-26676 [Abstract] [Full Text]
King, C., Eisenberg, E., Greene, L. (1995). Polymerization of 70-kDa Heat Shock Protein by Yeast DnaJ in ATP. J. Biol. Chem. 270: 22535-22540 [Abstract] [Full Text]
Kimura, Y, Yahara, I, Lindquist, S (1995). Role of the protein chaperone YDJ1 in establishing Hsp90-mediated signal transduction pathways. Science 268: 1362-1365 [Abstract]
Caplan, A. J., Langley, E., Wilson, E. M., Vidal, J. (1995). Hormone-dependent Transactivation by the Human Androgen Receptor Is Regulated by a dnaJ Protein. J. Biol. Chem. 270: 5251-5257 [Abstract] [Full Text]
Hattori, H, Kaneda, T, Lokeshwar, B, Laszlo, A, Ohtsuka, K (1993). A stress-inducible 40 kDa protein (hsp40): purification by modified two-dimensional gel electrophoresis and co-localization with hsc70(p73) in heat-shocked HeLa cells. J. Cell Sci. 104: 629-638 [Abstract]
Horton, L. E., James, P., Craig, E. A., Hensold, J. O. (2001). The Yeast hsp70 Homologue Ssa Is Required for Translation and Interacts with Sis1 and Pab1 on Translating Ribosomes. J. Biol. Chem. 276: 14426-14433 [Abstract] [Full Text]