Overexpression of tau in a nonneuronal cell induces long cellular processes

doi:10.1083/jcb.114.4.725

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Overexpression of tau in a nonneuronal cell induces long cellular processes

J Knops, KS Kosik, G Lee, JD Pardee, L Cohen-Gould and L McConlogue
Athena Neuroscience Inc., San Francisco, California.

The ways in which the various microtubule-associated proteins (MAPs) contribute to cellular function are unknown beyond the ability of these proteins to modify microtubule dynamics. One member of the MAP family, tau protein, is restricted in its distribution to the axonal compartment of neurons, and has therefore prompted studies that attempt to relate tau function to the generation or maintenance of this structure. Sf9 cells from a moth ovary, when infected with a baculovirus containing a tau cDNA insert, elaborate very long processes. This single gene product expressed in a foreign host cell grossly alters the normal rounded morphology of these cells. The slender, relatively nonbranched appearance of these processes as well as their uniform caliber resembles the light-microscopic appearance of axons observed in several neuronal culture systems. Immunolabeling of the tau-expressing Sf9 cells demonstrated tau reactivity in the induced processes, and EM that microtubule bundles were present in the processes. Microtubule stabilization alone was insufficient to generate processes, since taxol treatment did not alter the overall cell shape, despite the induction of microtubule bundling within the cell body.
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Farah, C. A., Liazoghli, D., Perreault, S., Desjardins, M., Guimont, A., Anton, A., Lauzon, M., Kreibich, G., Paiement, J., Leclerc, N. (2005). Interaction of Microtubule-associated Protein-2 and p63: A NEW LINK BETWEEN MICROTUBULES AND ROUGH ENDOPLASMIC RETICULUM MEMBRANES IN NEURONS. J. Biol. Chem. 280: 9439-9449 [Abstract] [Full Text]
Samsonov, A., Yu, J.-Z., Rasenick, M., Popov, S. V. (2004). Tau interaction with microtubules in vivo. J. Cell Sci. 117: 6129-6141 [Abstract] [Full Text]
Bunker, J. M., Wilson, L., Jordan, M. A., Feinstein, S. C. (2004). Modulation of Microtubule Dynamics by Tau in Living Cells: Implications for Development and Neurodegeneration. Mol. Biol. Cell 15: 2720-2728 [Abstract] [Full Text]
AVILA, J., LUCAS, J. J., PEREZ, M., HERNANDEZ, F. (2004). Role of Tau Protein in Both Physiological and Pathological Conditions. Physiol. Rev. 84: 361-384 [Abstract] [Full Text]
Krishnamurthy, P. K., Johnson, G. V. W. (2004). Mutant (R406W) Human Tau Is Hyperphosphorylated and Does Not Efficiently Bind Microtubules in a Neuronal Cortical Cell Model. J. Biol. Chem. 279: 7893-7900 [Abstract] [Full Text]
Makrides, V., Shen, T. E., Bhatia, R., Smith, B. L., Thimm, J., Lal, R., Feinstein, S. C. (2003). Microtubule-dependent Oligomerization of Tau: IMPLICATIONS FOR PHYSIOLOGICAL TAU FUNCTION AND TAUOPATHIES. J. Biol. Chem. 278: 33298-33304 [Abstract] [Full Text]
Biernat, J., Wu, Y.-Z., Timm, T., Zheng-Fischhofer, Q., Mandelkow, E., Meijer, L., Mandelkow, E.-M. (2002). Protein Kinase MARK/PAR-1 Is Required for Neurite Outgrowth and Establishment of Neuronal Polarity. Mol. Biol. Cell 13: 4013-4028 [Abstract] [Full Text]
Dawson, H., Ferreira, A, Eyster, M., Ghoshal, N, Binder, L., Vitek, M. (2001). Inhibition of neuronal maturation in primary hippocampal neurons from &tgr; deficient mice. J. Cell Sci. 114: 1179-1187 [Abstract]
Hall, G., Chu, B, Lee, G, Yao, J (2000). Human tau filaments induce microtubule and synapse loss in an in vivo model of neurofibrillary degenerative disease. J. Cell Sci. 113: 1373-1387 [Abstract]
Davis, P. K., Johnson, G. V. W. (1999). The Microtubule Binding of Tau and High Molecular Weight Tau in Apoptotic PC12 Cells Is Impaired because of Altered Phosphorylation. J. Biol. Chem. 274: 35686-35692 [Abstract] [Full Text]
Biernat, J., Mandelkow, E.-M. (1999). The Development of Cell Processes Induced by tau Protein Requires Phosphorylation of Serine 262�and 356�in the Repeat Domain and Is Inhibited by Phosphorylation in the Proline-rich Domains. Mol. Biol. Cell 10: 727-740 [Abstract] [Full Text]
Liao, H., Li, Y., Brautigan, D. L., Gundersen, G. G. (1998). Protein Phosphatase 1�Is Targeted to Microtubules by the Microtubule-associated Protein Tau. J. Biol. Chem. 273: 21901-21908 [Abstract] [Full Text]
Elliott, G., O'Hare, P. (1998). Herpes Simplex Virus Type 1�Tegument Protein VP22 Induces the Stabilization and Hyperacetylation of Microtubules. J. Virol. 72: 6448-6455 [Abstract] [Full Text]
Felgner, H., Frank, R., Biernat, J., Mandelkow, E.-M., Mandelkow, E., Ludin, B., Matus, A., Schliwa, M. (1997). Domains of Neuronal Microtubule-associated Proteins and Flexural Rigidity of Microtubules. JCB 138: 1067-1075 [Abstract] [Full Text]
Takei, Y., Kondo, S., Harada, A., Inomata, S., Noda, T., Hirokawa, N. (1997). Delayed Development of Nervous System in Mice Homozygous for Disrupted Microtubule-associated Protein 1B (MAP1B) Gene. JCB 137: 1615-1626 [Abstract] [Full Text]
Cunningham, C. C., Leclerc, N., Flanagan, L. A., Lu, M., Janmey, P. A., Kosik, K. S. (1997). Microtubule-associated Protein 2c Reorganizes Both Microtubules and Microfilaments into Distinct Cytological Structures in an Actin-binding Protein-280-deficient Melanoma Cell Line. JCB 136: 845-857 [Abstract] [Full Text]
Yu, W., Sharp, D. J., Kuriyama, R., Mallik, P., Baas, P. W. (1997). Inhibition of a Mitotic Motor Compromises the Formation of Dendrite-like Processes from Neuroblastoma Cells. JCB 136: 659-668 [Abstract] [Full Text]
Sharp, D., Kuriyama, R, Essner, R, Baas, P. (1997). Expression of a minus-end-directed motor protein induces Sf9 cells to form axon-like processes with uniform microtubule polarity orientation. J. Cell Sci. 110: 2373-2380 [Abstract]
Preuss, U, Biernat, J, Mandelkow, E., Mandelkow, E (1997). The 'jaws' model of tau-microtubule interaction examined in CHO cells. J. Cell Sci. 110: 789-800 [Abstract]
Kempf, M., Clement, A., Faissner, A., Lee, G., Brandt, R. (1996). Tau Binds to the Distal Axon Early in Development of Polarity in a Microtubule- and Microfilament-Dependent Manner. J. Neurosci. 16: 5583-5592 [Abstract] [Full Text]
Mandell, J. W., Banker, G. A. (1996). A Spatial Gradient of Tau Protein Phosphorylation in Nascent Axons. J. Neurosci. 16: 5727-5740 [Abstract] [Full Text]
Sharp, D. J., Kuriyama, R., Baas, P. W. (1996). Expression of a Kinesin-Related Motor Protein Induces Sf9 Cells to Form Dendrite-Like Processes with Nonuniform Microtubule Polarity Orientation. J. Neurosci. 16: 4370-4375 [Abstract] [Full Text]
Black, M. M., Slaughter, T., Moshiach, S., Obrocka, M., Fischer, I. (1996). Tau Is Enriched on Dynamic Microtubules in the Distal Region of Growing Axons. J. Neurosci. 16: 3601-3619 [Abstract] [Full Text]
Goedert, M, Baur, C., Ahringer, J, Jakes, R, Hasegawa, M, Spillantini, M., Smith, M., Hill, F (1996). PTL-1, a microtubule-associated protein with tau-like repeats from the nematode Caenorhabditis elegans. J. Cell Sci. 109: 2661-2672 [Abstract]
Zachow, K., Bentley, D (1996). Blackjack, a novel protein associated with microtubules in embryonic neurons. J. Cell Sci. 109: 1497-1507 [Abstract]
Wagner, U, Utton, M, Gallo, J., Miller, C. (1996). Cellular phosphorylation of tau by GSK-3 beta influences tau binding to microtubules and microtubule organisation. J. Cell Sci. 109: 1537-1543 [Abstract]
DiTella, M., Feiguin, F, Carri, N, Kosik, K., Caceres, A (1996). MAP-1B/TAU functional redundancy during laminin-enhanced axonal growth. J. Cell Sci. 109: 467-477 [Abstract]
Kuriyama, R, Dragas-Granoic, S, Maekawa, T, Vassilev, A, Khodjakov, A, Kobayashi, H (1994). Heterogeneity and microtubule interaction of the CHO1 antigen, a mitosis-specific kinesin-like protein. Analysis of subdomains expressed in insect sf9 cells. J. Cell Sci. 107: 3485-3499 [Abstract]
Ferralli, J, Doll, T, Matus, A (1994). Sequence analysis of MAP2 function in living cells. J. Cell Sci. 107: 3115-3125 [Abstract]
Audebert, S, Koulakoff, A, Berwald-Netter, Y, Gros, F, Denoulet, P, Edde, B (1994). Developmental regulation of polyglutamylated alpha- and beta-tubulin in mouse brain neurons. J. Cell Sci. 107: 2313-2322 [Abstract]
Esmaeli-Azad, B, McCarty, J., Feinstein, S. (1994). Sense and antisense transfection analysis of tau function: tau influences net microtubule assembly, neurite outgrowth and neuritic stability. J. Cell Sci. 107: 869-879 [Abstract]
Baas, P., Pienkowski, T., Cimbalnik, K., Toyama, K, Bakalis, S, Ahmad, F., Kosik, K. (1994). Tau confers drug stability but not cold stability to microtubules in living cells. J. Cell Sci. 107: 135-143 [Abstract]
Teng, K., Georgieff, I., Aletta, J., Nunez, J, Shelanski, M., Greene, L. (1993). Characterization of a PC12 cell sub-clone (PC12-C41) with enhanced neurite outgrowth capacity: implications for a modulatory role of high molecular weight tau in neuritogenesis. J. Cell Sci. 106: 611-626 [Abstract]
Georgieff, I., Liem, R., Couchie, D, Mavilia, C, Nunez, J, Shelanski, M. (1993). Expression of high molecular weight tau in the central and peripheral nervous systems. J. Cell Sci. 105: 729-737 [Abstract]
Edson, K, Weisshaar, B, Matus, A (1993). Actin depolymerisation induces process formation on MAP2-transfected non-neuronal cells. Development 117: 689-700 [Abstract]
Kosik, K. (1992). Alzheimer's disease: a cell biological perspective. Science 256: 780-783 [Abstract]