Substratum-growth factor collaborations are required for the mitogenic activities of activin and FGF on embryonal carcinoma cells

doi:10.1083/jcb.114.4.841

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Substratum-growth factor collaborations are required for the mitogenic activities of activin and FGF on embryonal carcinoma cells

D Schubert and H Kimura
Salk Institute, San Diego, California 92186-5800.

When P19 mouse embryonal carcinoma cells are grown in a serum-free N2 medium on surfaces of tissue culture plastic, they die within two days. The death of these P19 cells is prevented by activin A and basic FGF (bFGF). The cells do not divide under these conditions. However, when P19 cells are cultured on substrata of extracellular matrix proteins such as laminin and fibronectin, activin A and bFGF are potent mitogens. These data show that the substratum to which cells are exposed can regulate their mitogenic response to growth factors.
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