A heterotrimeric G protein, G alpha i-3, on Golgi membranes regulates the secretion of a heparan sulfate proteoglycan in LLC-PK1 epithelial cells

doi:10.1083/jcb.114.6.1113

This Article

	Full Text (PDF, 2153K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Stow, J. L.
	Articles by Ausiello, D. A.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Stow, J. L.
	Articles by Ausiello, D. A.


Social Bookmarking

	What's this?

ARTICLES

A heterotrimeric G protein, G alpha i-3, on Golgi membranes regulates the secretion of a heparan sulfate proteoglycan in LLC-PK1 epithelial cells

JL Stow, JB de Almeida, N Narula, EJ Holtzman, L Ercolani and DA Ausiello
Renal Unit, Massachusetts General Hospital, Boston.

A heterotrimeric G alpha i subunit, alpha i-3, is localized on Golgi membranes in LLC-PK1 and NRK epithelial cells where it colocalizes with mannosidase II by immunofluorescence. The alpha i-3 was found to be localized on the cytoplasmic face of Golgi cisternae and it was distributed across the whole Golgi stack. The alpha i-3 subunit is found on isolated rat liver Golgi membranes by Western blotting and G alpha i-3 on the Golgi apparatus is ADP ribosylated by pertussis toxin. LLC-PK1 cells were stably transfected with G alpha i-3 on an MT-1, inducible promoter in order to overexpress alpha i-3 on Golgi membranes. The intracellular processing and constitutive secretion of the basement membrane heparan sulfate proteoglycan (HSPG) was measured in LLC-PK1 cells. Overexpression of alpha i-3 on Golgi membranes in transfected cells retarded the secretion of HSPG and accumulated precursors in the medial-trans-Golgi. This effect was reversed by treatment of cells with pertussis toxin which results in ADP- ribosylation and functional uncoupling of G alpha i-3 on Golgi membranes. These results provide evidence for a novel role for the pertussis toxin sensitive G alpha i-3 protein in Golgi trafficking of a constitutively secreted protein in epithelial cells.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Mir, F., Le Breton, G. C. (2008). A Novel Nuclear Signaling Pathway for Thromboxane A2 Receptors in Oligodendrocytes: Evidence for Signaling Compartmentalization during Differentiation. Mol. Cell. Biol. 28: 6329-6341 [Abstract] [Full Text]
An, N., Blumer, J. B., Bernard, M. L., Lanier, S. M. (2008). The PDZ and Band 4.1 Containing Protein Frmpd1 Regulates the Subcellular Location of Activator of G-protein Signaling 3 and Its Interaction with G-proteins. J. Biol. Chem. 283: 24718-24728 [Abstract] [Full Text]
Ghosh, P., Garcia-Marcos, M., Bornheimer, S. J., Farquhar, M. G. (2008). Activation of G{alpha}i3 triggers cell migration via regulation of GIV. JCB 182: 381-393 [Abstract] [Full Text]
Sabath, E., Negoro, H., Beaudry, S., Paniagua, M., Angelow, S., Shah, J., Grammatikakis, N., Yu, A. S. L., Denker, B. M. (2008). G{alpha}12 regulates protein interactions within the MDCK cell tight junction and inhibits tight-junction assembly. J. Cell Sci. 121: 814-824 [Abstract] [Full Text]
Groves, B., Gong, Q., Xu, Z., Huntsman, C., Nguyen, C., Li, D., Ma, D. (2007). A specific role of AGS3 in the surface expression of plasma membrane proteins. Proc. Natl. Acad. Sci. USA 104: 18103-18108 [Abstract] [Full Text]
Cho, H., Kehrl, J. H. (2007). Localization of Gi{alpha} proteins in the centrosomes and at the midbody: implication for their role in cell division. JCB 178: 245-255 [Abstract] [Full Text]
Le-Niculescu, H., Niesman, I., Fischer, T., DeVries, L., Farquhar, M. G. (2005). Identification and Characterization of GIV, a Novel G{alpha}i/s -interacting Protein Found on COPI, Endoplasmic Reticulum-Golgi Transport Vesicles. J. Biol. Chem. 280: 22012-22020 [Abstract] [Full Text]
Robay, A., Toumaniantz, G., Leblais, V., Gauthier, C. (2005). Transfected {beta}3- but Not {beta}2-Adrenergic Receptors Regulate Cystic Fibrosis Transmembrane Conductance Regulator Activity via a New Pathway Involving the Mitogen-Activated Protein Kinases Extracellular Signal-Regulated Kinases. Mol. Pharmacol. 67: 648-654 [Abstract] [Full Text]
Cao, X., Cismowski, M. J., Sato, M., Blumer, J. B., Lanier, S. M. (2004). Identification and Characterization of AGS4: A PROTEIN CONTAINING THREE G-PROTEIN REGULATORY MOTIFS THAT REGULATE THE ACTIVATION STATE OF Gi{alpha}. J. Biol. Chem. 279: 27567-27574 [Abstract] [Full Text]
Chan, D., Strang, M., Judson, B., Brown, W. J. (2004). Inhibition of Membrane Tubule Formation and Trafficking by Isotetrandrine, an Antagonist of G-protein-regulated Phospholipase A2 Enzymes. Mol. Biol. Cell 15: 1871-1880 [Abstract] [Full Text]
Kaushik, R., Yu, F., Chia, W., Yang, X., Bahri, S. (2003). Subcellular Localization of LGN During Mitosis: Evidence for Its Cortical Localization in Mitotic Cell Culture Systems and Its Requirement for Normal Cell Cycle Progression. Mol. Biol. Cell 14: 3144-3155 [Abstract] [Full Text]
Pattingre, S., De Vries, L., Bauvy, C., Chantret, I., Cluzeaud, F., Ogier-Denis, E., Vandewalle, A., Codogno, P. (2003). The G-protein Regulator AGS3 Controls an Early Event during Macroautophagy in Human Intestinal HT-29 Cells. J. Biol. Chem. 278: 20995-21002 [Abstract] [Full Text]
SARMA, T., VOYNO-YASENETSKAYA, T., HOPE, T. J., RASENICK, M. M. (2003). Heterotrimeric G-proteins associate with microtubules during differentiation in PC12 pheochromocytoma cells. FASEB J. 17: 848-859 [Abstract] [Full Text]
Brown, D. (2003). The ins and outs of aquaporin-2 trafficking. Am. J. Physiol. Renal Physiol. 284: F893-F901 [Abstract] [Full Text]
Weiss, T. S., Chamberlain, C. E., Takeda, T., Lin, P., Hahn, K. M., Farquhar, M. G. (2001). Galpha i3 binding to calnuc on Golgi membranes in living cells monitored by fluorescence resonance energy transfer of green fluorescent protein fusion proteins. Proc. Natl. Acad. Sci. USA 98: 14961-14966 [Abstract] [Full Text]
Lampe, P. D., Qiu, Q., Meyer, R. A., TenBroek, E. M., Walseth, T. F., Starich, T. A., Grunenwald, H. L., Johnson, R. G. (2001). Gap junction assembly: PTX-sensitive G proteins regulate the distribution of connexin43 within cells. Am. J. Physiol. Cell Physiol. 281: C1211-C1222 [Abstract] [Full Text]
Gkantiragas, I., Brugger, B., Stuven, E., Kaloyanova, D., Li, X.-Y., Lohr, K., Lottspeich, F., Wieland, F. T., Helms, J. B. (2001). Sphingomyelin-enriched Microdomains at the Golgi Complex. Mol. Biol. Cell 12: 1819-1833 [Abstract] [Full Text]
Roseberry, A. G., Bünemann, M., Elavunkal, J., Hosey, M. M. (2001). Agonist-Dependent Delivery of M2 Muscarinic Acetylcholine Receptors to the Cell Surface after Pertussis Toxin Treatment. Mol. Pharmacol. 59: 1256-1268 [Abstract] [Full Text]
Sullivan, B. M., Harrison-Lavoie, K. J., Marshansky, V., Lin, H. Y., Kehrl, J. H., Ausiello, D. A., Brown, D., Druey, K. M. (2000). RGS4 and RGS2 Bind Coatomer and Inhibit COPI Association with Golgi Membranes and Intracellular Transport. Mol. Biol. Cell 11: 3155-3168 [Abstract] [Full Text]
Lin, P., Fischer, T., Weiss, T., Farquhar, M. G. (2000). Calnuc, an EF-Hand Ca2+ binding protein, specifically interacts with the C-terminal alpha 5-helix of Galpha i3. Proc. Natl. Acad. Sci. USA 97: 674-679 [Abstract] [Full Text]
Luo, Y., Denker, B. M. (1999). Interaction of Heterotrimeric G Protein Galpha o with Purkinje Cell Protein-2. EVIDENCE FOR A NOVEL NUCLEOTIDE EXCHANGE FACTOR. J. Biol. Chem. 274: 10685-10688 [Abstract] [Full Text]
Wylie, F., Heimann, K., Le, T. L., Brown, D., Rabnott, G., Stow, J. L. (1999). GAIP, a Galpha i-3-binding protein, is associated with Golgi-derived vesicles and protein trafficking. Am. J. Physiol. Cell Physiol. 276: C497-C506 [Abstract] [Full Text]
Martin, M., Hidalgo, J, Vega, F., Velasco, A (1999). Trimeric G proteins modulate the dynamic interaction of PKAII with the Golgi complex. J. Cell Sci. 112: 3869-3878 [Abstract]
Kolset, S., Vuong, T., Prydz, K (1999). Apical secretion of chondroitin sulphate in polarized Madin-Darby canine kidney (MDCK) cells. J. Cell Sci. 112: 1797-1801 [Abstract]
Babia, T, Ayala, I, Valderrama, F, Mato, E, Bosch, M, Santaren, J., Renau-Piqueras, J, Kok, J., Thomson, T., Egea, G (1999). N-Ras induces alterations in Golgi complex architecture and in constitutive protein transport. J. Cell Sci. 112: 477-489 [Abstract]
Merlet, F., Weinstein, L. S., Goldsmith, P. K., Rarick, T., Hall, J. L., Bisson, J.-P., de Mazancourt, P. (1999). Identification and localization of G protein subunits in human spermatozoa. Mol Hum Reprod 5: 38-45 [Abstract] [Full Text]
Kinane, T. B., Komatsuzaki, K., Aleixo, M. D., Sunday, M. E., Ercolani, L. (1999). Regulation of the G Protein Galpha i2 by Growth and Development in Fetal Airway Epithelium. Am. J. Respir. Cell Mol. Bio. 20: 35-42 [Abstract] [Full Text]
Valenti, G., Procino, G., Liebenhoff, U., Frigeri, A., Benedetti, P. A., Ahnert-Hilger, G., Nurnberg, B., Svelto, M., Rosenthal, W. (1998). A Heterotrimeric G Protein of the Gi Family Is Required for cAMP-triggered Trafficking of Aquaporin 2�in Kidney Epithelial Cells. J. Biol. Chem. 273: 22627-22634 [Abstract] [Full Text]
Druey, K. M., Sullivan, B. M., Brown, D., Fischer, E. R., Watson, N., Blumer, K. J., Gerfen, C. R., Scheschonka, A., Kehrl, J. H. (1998). Expression of GTPase-deficient Gialpha 2 Results in Translocation of Cytoplasmic RGS4 to the Plasma Membrane. J. Biol. Chem. 273: 18405-18410 [Abstract] [Full Text]
Helms, J. B., Helms-Brons, D., Brugger, B., Gkantiragas, I., Eberle, H., Nickel, W., Nurnberg, B., Gerdes, H.-H., Wieland, F. T. (1998). A Putative Heterotrimeric G Protein Inhibits the Fusion of COPI-coated Vesicles. SEGREGATION OF HETEROTRIMERIC G PROTEINS FROM COPI-COATED VESICLES. J. Biol. Chem. 273: 15203-15208 [Abstract] [Full Text]
De Vries, L., Elenko, E., McCaffery, J. M., Fischer, T., Hubler, L., McQuistan, T., Watson, N., Farquhar, M. G. (1998). RGS-GAIP, a GTPase-activating Protein for Galpha i Heterotrimeric G Proteins, Is Located on Clathrin-coated Vesicles. Mol. Biol. Cell 9: 1123-1134 [Abstract] [Full Text]
Nickel, W, Malsam, J, Gorgas, K, Ravazzola, M, Jenne, N, Helms, J., Wieland, F. (1998). Uptake by COPI-coated vesicles of both anterograde and retrograde cargo is inhibited by GTPgammaS in vitro. J. Cell Sci. 111: 3081-3090 [Abstract]
Yamaguchi, T., Yamamoto, A., Furuno, A., Hatsuzawa, K., Tani, K., Himeno, M., Tagaya, M. (1997). Possible Involvement of Heterotrimeric G Proteins in the Organization of the Golgi Apparatus. J. Biol. Chem. 272: 25260-25266 [Abstract] [Full Text]
Liu, J., Hughes, T. E., Sessa, W. C. (1997). The First 35 Amino Acids and Fatty Acylation Sites Determine the Molecular Targeting of Endothelial Nitric Oxide Synthase into the Golgi Region of Cells: A Green Fluorescent Protein Study. JCB 137: 1525-1535 [Abstract] [Full Text]
Ohnishi, H., Ernst, S. A., Yule, D. I., Baker, C. W., Williams, J. A. (1997). Heterotrimeric G-protein Gq/11 Localized on Pancreatic Zymogen Granules Is Involved in Calcium-regulated Amylase Secretion. J. Biol. Chem. 272: 16056-16061 [Abstract] [Full Text]
Beau, I., Misrahi, M., Gross, B., Vannier, B., Loosfelt, H., Hai, M. T. V., Pichon, C., Milgrom, E. (1997). Basolateral Localization and Transcytosis of Gonadotropin and Thyrotropin Receptors Expressed in Madin-Darby Canine Kidney Cells. J. Biol. Chem. 272: 5241-5248 [Abstract] [Full Text]
Ueda, H, Saga, S, Shinohara, H, Morishita, R, Kato, K, Asano, T (1997). Association of the gamma12 subunit of G proteins with actin filaments. J. Cell Sci. 110: 1503-1511 [Abstract]
Lagriffoul, A., Charpentier, N., Carrette, J., Tougard, C., Bockaert, J., Homburger, V. (1996). Secretion of Protease Nexin-1 by C6 Glioma Cells Is under the Control of a Heterotrimeric G Protein, Go1. J. Biol. Chem. 271: 31508-31516 [Abstract] [Full Text]
Muniz, M., Alonso, M., Hidalgo, J., Velasco, A. (1996). A Regulatory Role for cAMP-dependent Protein Kinase in Protein Traffic along the Exocytic Route. J. Biol. Chem. 271: 30935-30941 [Abstract] [Full Text]
Ogier-Denis, E., Houri, J.-J., Bauvy, C., Codogno, P. (1996). Guanine Nucleotide Exchange on Heterotrimeric Gi3 Protein Controls Autophagic Sequestration in HT-29 Cells. J. Biol. Chem. 271: 28593-28600 [Abstract] [Full Text]
Denker, B. M., Saha, C., Khawaja, S., Nigam, S. K. (1996). Involvement of a Heterotrimeric G Protein alpha Subunit in Tight Junction Biogenesis. J. Biol. Chem. 271: 25750-25753 [Abstract] [Full Text]
Mayer, T., Touchot, N., Elazar, Z. (1996). Transport between cis and medial Golgi Cisternae Requires the Function of the Ras-related Protein Rab6. J. Biol. Chem. 271: 16097-16103 [Abstract] [Full Text]
Buccione, R., Bannykh, S., Santone, I., Baldassarre, M., Facchiano, F., Bozzi, Y., Di Tullio, G., Mironov, A., Luini, A., De Matteis, M. A. (1996). Regulation of Constitutive Exocytic Transport by Membrane Receptors. J. Biol. Chem. 271: 3523-3533 [Abstract] [Full Text]
Gleeson, P., Anderson, T., Stow, J., Griffiths, G, Toh, B., Matheson, F (1996). p230 is associated with vesicles budding from the trans-Golgi network. J. Cell Sci. 109: 2811-2821 [Abstract]
Voisin, T., Lorinet, A.-M., Maoret, J.-J., Couvineau, A., Laburthe, M. (1996). Galpha(i) RNA Antisense Expression Demonstrates the Exclusive Coupling of Peptide YY Receptors to G[IMAGE] Proteins in Renal Proximal Tubule Cells. J. Biol. Chem. 271: 574-580 [Abstract] [Full Text]
Lowe, S., Wong, S., Hong, W (1996). The mammalian ARF-like protein 1 (Arl1) is associated with the Golgi complex. J. Cell Sci. 109: 209-220 [Abstract]
Colombo, M. I., Inglese, J., D'Souza-Schorey, C., Beron, W., Stahl, P. D. (1995). Heterotrimeric G Proteins Interact with the Small GTPase ARF. J. Biol. Chem. 270: 24564-24571 [Abstract] [Full Text]
Chun, M., Lin, H. Y., Henis, Y. I., Lodish, H. F. (1995). Endothelin-induced Endocytosis of Cell Surface ET[IMAGE] Receptors. J. Biol. Chem. 270: 10855-10860 [Abstract] [Full Text]
Ogier-Denis, E., Couvineau, A., Maoret, J. J., Houri, J. J., Bauvy, C., De Stefanis, D., Isidoro, C., Laburthe, M., Codogno, P. (1995). A Heterotrimeric G[IMAGE]-protein Controls Autophagic Sequestration in the Human Colon Cancer Cell Line HT-29. J. Biol. Chem. 270: 13-16 [Abstract] [Full Text]
Brewer, C., Roth, M. (1995). Polarized exocytosis in MDCK cells is regulated by phosphorylation. J. Cell Sci. 108: 789-796 [Abstract]
Dharmawardhane, S, Cubitt, A., Clark, A., Firtel, R. (1994). Regulatory role of the G alpha 1 subunit in controlling cellular morphogenesis in Dictyostelium. Development 120: 3549-3561 [Abstract]
Montmayeur, J., Borrelli, E (1994). Targeting of G alpha i2 to the Golgi by alternative spliced carboxyl-terminal region. Science 263: 95-98 [Abstract]
de Almeida, J., Holtzman, E., Peters, P, Ercolani, L, Ausiello, D., Stow, J. (1994). Targeting of chimeric G alpha i proteins to specific membrane domains. J. Cell Sci. 107: 507-515 [Abstract]
de Almeida, J., Doherty, J, Ausiello, D., Stow, J. (1993). Binding of the cytosolic p200 protein to Golgi membranes is regulated by heterotrimeric G proteins. J. Cell Sci. 106: 1239-1248 [Abstract]
Roa, M, Cornet, V, Yang, C., Goud, B (1993). The small GTP-binding protein rab6p is redistributed in the cytosol by brefeldin A. J. Cell Sci. 106: 789-802 [Abstract]
Rennison, M., Kerr, M, Addey, C., Handel, S., Turner, M., Wilde, C., Burgoyne, R. (1993). Inhibition of constitutive protein secretion from lactating mouse mammary epithelial cells by FIL (feedback inhibitor of lactation), a secreted milk protein. J. Cell Sci. 106: 641-648 [Abstract]
White, J. (1992). Membrane fusion. Science 258: 917-924 [Abstract]
Colombo, M., Mayorga, L., Casey, P., Stahl, P. (1992). Evidence of a role for heterotrimeric GTP-binding proteins in endosome fusion. Science 255: 1695-1697 [Abstract]
Tang, W.-J., Iniguez-Lluhi, J.A., Mumby, S., Gilman, A.G. (1992). Regulation of Mammalian Adenylyl Cyclases by G-Protein {alpha} and {beta}{gamma} Subunits. Cold Spring Harb Symp Quant Biol 57: 135-144 [Abstract]
Miranda, K. C., Khromykh, T., Christy, P., Le, T. L., Gottardi, C. J., Yap, A. S., Stow, J. L., Teasdale, R. D. (2001). A Dileucine Motif Targets E-cadherin to the Basolateral Cell Surface in Madin-Darby Canine Kidney and LLC-PK1 Epithelial Cells. J. Biol. Chem. 276: 22565-22572 [Abstract] [Full Text]