Microtubule sliding in swimming sperm flagella: direct and indirect measurements on sea urchin and tunicate spermatozoa [published erratum appears in J Cell Biol 1991 Nov;115(4):1204]

doi:10.1083/jcb.114.6.1201

This Article

	Full Text (PDF, 1730K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Brokaw, C. J.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Brokaw, C. J.

Social Bookmarking

	What's this?

ARTICLES

Microtubule sliding in swimming sperm flagella: direct and indirect measurements on sea urchin and tunicate spermatozoa [published erratum appears in J Cell Biol 1991 Nov;115(4):1204]

CJ Brokaw
Division of Biology, California Institute of Technology, Pasadena 91125.

Direct measurements of microtubule sliding in the flagella of actively swimming, demembranated, spermatozoa have been made using submicron diameter gold beads as markers on the exposed outer doublet microtubules. With spermatozoa of the tunicate, Ciona, these measurements confirm values of sliding calculated indirectly by measuring angles relative to the axis of the sperm head. Both methods of measurement show a nonuniform amplitude of oscillatory sliding along the length of the flagellum, providing direct evidence that "oscillatory synchronous sliding" can be occurring in the flagellum, in addition to the metachronous sliding that is necessary to propagate a bending wave. Propagation of constant amplitude bends is not accomplished by propagation of a wave of oscillatory sliding of constant amplitude, and therefore appears to require a mechanism for monitoring and controlling the bend angle as bends propagate. With sea urchin spermatozoa, the direct measurements of sliding do not agree with the values calculated by measuring angles relative to the head axis. The oscillation in angular orientation of the sea urchin sperm head as it swims appears to be accommodated by flexure at the head- flagellum junction and does not correspond to oscillation in orientation of the basal end of the flagellum. Consequently, indirect calculations of sliding based on angles measured relative to the longitudinal axis of the sperm head can be seriously inaccurate in this species.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Gokhale, A., Wirschell, M., Sale, W. S. (2009). Regulation of dynein-driven microtubule sliding by the axonemal protein kinase CK1 in Chlamydomonas flagella. JCB 186: 817-824 [Abstract] [Full Text]
Zilli, L., Schiavone, R., Storelli, C., Vilella, S. (2008). Molecular Mechanisms Determining Sperm Motility Initiation in Two Sparids (Sparus aurata and Lithognathus mormyrus). Biol. Reprod. 79: 356-366 [Abstract] [Full Text]
Gaillard, A. R., Fox, L. A., Rhea, J. M., Craige, B., Sale, W. S. (2006). Disruption of the A-Kinase Anchoring Domain in Flagellar Radial Spoke Protein 3 Results in Unregulated Axonemal cAMP-dependent Protein Kinase Activity and Abnormal Flagellar Motility. Mol. Biol. Cell 17: 2626-2635 [Abstract] [Full Text]
Nicastro, D., McIntosh, J. R., Baumeister, W. (2005). 3D structure of eukaryotic flagella in a quiescent state revealed by cryo-electron tomography. Proc. Natl. Acad. Sci. USA 102: 15889-15894 [Abstract] [Full Text]
Ikeda, K., Brown, J. A., Yagi, T., Norrander, J. M., Hirono, M., Eccleston, E., Kamiya, R., Linck, R. W. (2003). Rib72, a Conserved Protein Associated with the Ribbon Compartment of Flagellar A-microtubules and Potentially Involved in the Linkage between Outer Doublet Microtubules. J. Biol. Chem. 278: 7725-7734 [Abstract] [Full Text]
Bannai, H, Yoshimura, M, Takahashi, K, Shingyoji, C (2000). Calcium regulation of microtubule sliding in reactivated sea urchin sperm flagella. J. Cell Sci. 113: 831-839 [Abstract]
Shingyoji, C, Takahashi, K (1995). Cyclical bending movements induced locally by successive iontophoretic application of ATP to an elastase-treated flagellar axoneme. J. Cell Sci. 108: 1359-1369 [Abstract]
Asai, D., Beckwith, S., Kandl, K., Keating, H., Tjandra, H, Forney, J. (1994). The dynein genes of Paramecium tetraurelia. Sequences adjacent to the catalytic P-loop identify cytoplasmic and axonemal heavy chain isoforms. J. Cell Sci. 107: 839-847 [Abstract]
Smith, E., Sale, W. (1992). Regulation of dynein-driven microtubule sliding by the radial spokes in flagella. Science 257: 1557-1559 [Abstract]