Calcium transients during Fc receptor-mediated and nonspecific phagocytosis by murine peritoneal macrophages

doi:10.1083/jcb.115.1.59

This Article

	Full Text (PDF, 1059K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Hishikawa, T.
	Articles by Knutson, D. W.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Hishikawa, T.
	Articles by Knutson, D. W.


Social Bookmarking

	What's this?

ARTICLES

Calcium transients during Fc receptor-mediated and nonspecific phagocytosis by murine peritoneal macrophages

T Hishikawa, JY Cheung, RV Yelamarty and DW Knutson
Department of Medicine, Pennsylvania State University College of Medicine, Milton S. Hershey Medical Center, Hershey 17033.

Studies with populations of macrophages have produced conflicting results concerning the possibility that the concentration of intracellular ionized calcium [( Ca2+]i) may act as an important mediator for phagocytosis. Since asynchronous changes in [Ca2+]i in individual cells undergoing phagocytosis may be averaged to undetectability in population studies, we studied single adhering murine macrophages using fura-2 and our previously described digital imaging system. The proportion of macrophages phagocytosing IgG-coated latex beads was greater than for uncoated beads (percent phagocytosing cells: 71 +/- 7 vs. 27 +/- 7, P less than 0.01). Phagocytosis of IgG- coated and uncoated beads was always associated with a calcium transient that preceded the initiation of phagocytosis. No calcium transients were detected in cells that bound but did not phagocytose beads. Four major differences between Fc receptor-mediated and nonspecific phagocytosis were detected: (a) the duration of calcium transients was longer for nonspecific phagocytosis compared with Fc receptor-mediated phagocytosis (69.9 +/- 10.2 vs. 48.7 +/- 4.7 s, P less than 0.05) and the magnitude of calcium transients was less for nonspecific phagocytosis (178 +/- 43 vs. 349 +/- 53 nM, P less than 0.05); (b) removal of extracellular calcium abolished the calcium transients associated with nonspecific phagocytosis but had no effect on those associated with receptor-mediated phagocytosis; (c) in the absence of extracellular calcium, buffering intracellular calcium with a chelator reduced Fc receptor-mediated phagocytosis but had no additive inhibitory effect on nonspecific phagocytosis; and (d) inhibition of protein kinase C (PKC) with staurosporine inhibited nonspecific phagocytosis but had no effect on receptor-mediated phagocytosis. Our observations suggest that despite both types of phagocytosis being associated with intracellular calcium transients, the role played by intracellular calcium in the signaling pathways may differ for Fc receptor-mediated and nonspecific phagocytosis by elicited murine macrophages.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Braun, A., Gessner, J. E., Varga-Szabo, D., Syed, S. N., Konrad, S., Stegner, D., Vogtle, T., Schmidt, R. E., Nieswandt, B. (2009). STIM1 is essential for Fc{gamma} receptor activation and autoimmune inflammation. Blood 113: 1097-1104 [Abstract] [Full Text]
Czibener, C., Sherer, N. M., Becker, S. M., Pypaert, M., Hui, E., Chapman, E. R., Mothes, W., Andrews, N. W. (2006). Ca2+ and synaptotagmin VII-dependent delivery of lysosomal membrane to nascent phagosomes. JCB 174: 997-1007 [Abstract] [Full Text]
Scott, C. C., Dobson, W., Botelho, R. J., Coady-Osberg, N., Chavrier, P., Knecht, D. A., Heath, C., Stahl, P., Grinstein, S. (2005). Phosphatidylinositol-4,5-bisphosphate hydrolysis directs actin remodeling during phagocytosis. JCB 169: 139-149 [Abstract] [Full Text]
Girotti, M., Evans, J. H., Burke, D., Leslie, C. C. (2004). Cytosolic Phospholipase A2 Translocates to Forming Phagosomes during Phagocytosis of Zymosan in Macrophages. J. Biol. Chem. 279: 19113-19121 [Abstract] [Full Text]
Nakamura, K., Malykhin, A., Coggeshall, K. M. (2002). The Src homology 2 domain-containing inositol 5-phosphatase negatively regulates Fcgamma receptor-mediated phagocytosis through immunoreceptor tyrosine-based activation motif-bearing phagocytic receptors. Blood 100: 3374-3382 [Abstract] [Full Text]
Colden-Stanfield, M. (2002). Clustering of very late antigen-4 integrins modulates K+ currents to alter Ca2+-mediated monocyte function. Am. J. Physiol. Cell Physiol. 283: C990-C1000 [Abstract] [Full Text]
Su, S.-H., Chen, H.-i., Jen, C. J. (2001). Severe exercise enhances phagocytosis by murine bronchoalveolar macrophages. J. Leukoc. Biol. 69: 75-80 [Abstract] [Full Text]
Botelho, R. J., Teruel, M., Dierckman, R., Anderson, R., Wells, A., York, J. D., Meyer, T., Grinstein, S. (2000). Localized Biphasic Changes in Phosphatidylinositol-4,5-Bisphosphate at Sites of Phagocytosis. JCB 151: 1353-1368 [Abstract] [Full Text]
Colden-Stanfield, M., Scanlon, M. (2000). VCAM-1-induced inwardly rectifying K+ current enhances Ca2+ entry in human THP-1 monocytes. Am. J. Physiol. Cell Physiol. 279: C488-C494 [Abstract] [Full Text]
Ohsawa, K, Imai, Y, Kanazawa, H, Sasaki, Y, Kohsaka, S (2000). Involvement of Iba1 in membrane ruffling and phagocytosis of macrophages/microglia. J. Cell Sci. 113: 3073-3084 [Abstract]
Kaposzta, R, Marodi, L, Hollinshead, M, Gordon, S, da Silva, R. (1999). Rapid recruitment of late endosomes and lysosomes in mouse macrophages ingesting Candida albicans. J. Cell Sci. 112: 3237-3248 [Abstract]
Edberg, J. C., Lin, C.-T., Lau, D., Unkeless, J. C., Kimberly, R. P. (1995). The Ca[IMAGE] Dependence of Human Fc[IMAGE] Receptor-initiated Phagocytosis. J. Biol. Chem. 270: 22301-22307 [Abstract] [Full Text]
Di, A., Krupa, B., Nelson, D. J. (2001). Calcium-G Protein Interactions in the Regulation of Macrophage Secretion. J. Biol. Chem. 276: 37124-37132 [Abstract] [Full Text]