Aurintricarboxylic acid rescues PC12 cells and sympathetic neurons from cell death caused by nerve growth factor deprivation: correlation with suppression of endonuclease activity

doi:10.1083/jcb.115.2.461

This Article

	Full Text (PDF, 1821K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Batistatou, A.
	Articles by Greene, L. A.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Batistatou, A.
	Articles by Greene, L. A.


Social Bookmarking

	What's this?

ARTICLES

Aurintricarboxylic acid rescues PC12 cells and sympathetic neurons from cell death caused by nerve growth factor deprivation: correlation with suppression of endonuclease activity

A Batistatou and LA Greene
Department of Pathology and Center for Neurobiology and Behavior, Columbia University, College of Physicians and Surgeons, New York 10032.

Past studies have shown that serum-free cultures of PC12 cells are a useful model system for studying the neuronal cell death which occurs after neurotrophic factor deprivation. In this experimental paradigm, nerve growth factor (NGF) rescues the cells from death. It is reported here that serum-deprived PC12 cells manifest an endonuclease activity that leads to internucleosomal cleavage of their cellular DNA. This activity is detected within 3 h of serum withdrawal and several hours before any morphological sign of cell degeneration or death. NGF and serum, which promote survival of the cells, inhibit the DNA fragmentation. Aurintricarboxylic acid (ATA), a general inhibitor of nucleases in vitro, suppresses the endonuclease activity and promotes long-term survival of PC12 cells in serum-free cultures. This effect appears to be independent of macromolecular synthesis. In addition, ATA promotes long-term survival of cultured sympathetic neurons after NGF withdrawal. ATA neither promotes nor maintains neurite outgrowth. It is hypothesized that the activation of an endogenous endonuclease could be responsible for neuronal cell death after neurotrophic factor deprivation and that growth factors could promote survival by leading to inhibition of constitutively present endonucleases.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Shigeta, M., Shibukawa, Y., Ihara, H., Miyoshi, E., Taniguchi, N., Gu, J. (2006). {beta}1,4-N-Acetylglucosaminyltransferase III potentiates {beta}1 integrin-mediated neuritogenesis induced by serum deprivation in Neuro2a cells. Glycobiology 16: 564-571 [Abstract] [Full Text]
Biswas, S. C., Liu, D. X., Greene, L. A. (2005). Bim Is a Direct Target of a Neuronal E2F-Dependent Apoptotic Pathway. J. Neurosci. 25: 8349-8358 [Abstract] [Full Text]
Liang, F., Huang, Z., Lee, S.-Y., Liang, J., Ivanov, M. I., Alonso, A., Bliska, J. B., Lawrence, D. S., Mustelin, T., Zhang, Z.-Y. (2003). Aurintricarboxylic Acid Blocks in Vitro and in Vivo Activity of YopH, an Essential Virulent Factor of Yersinia pestis, the Agent of Plague. J. Biol. Chem. 278: 41734-41741 [Abstract] [Full Text]
Tsi, C.-J., Chao, Y., Chen, C.-W., Lin, W. W. (2002). Aurintricarboxylic Acid Protects against Cell Death Caused by Lipopolysaccharide in Macrophages by Decreasing Inducible Nitric-Oxide Synthase Induction via Ikappa B Kinase, Extracellular Signal-Regulated Kinase, and p38 Mitogen-Activated Protein Kinase Inhibition. Mol. Pharmacol. 62: 90-101 [Abstract] [Full Text]
Tatton, W. G., Chalmers-Redman, R. M. E., Ju, W. J. H., Mammen, M., Carlile, G. W., Pong, A. W., Tatton, N. A. (2002). Propargylamines Induce Antiapoptotic New Protein Synthesis in Serum- and Nerve Growth Factor (NGF)-Withdrawn, NGF-Differentiated PC-12 Cells. J. Pharmacol. Exp. Ther. 301: 753-764 [Abstract] [Full Text]
Haimsohn, M., Beery, R., Karasik, A., Kanety, H., Geier, A. (2002). Aurintricarboxylic Acid Induces a Distinct Activation of the IGF-I Receptor Signaling within MDA-231 Cells. Endocrinology 143: 837-845 [Abstract] [Full Text]
Perkins, D., Pereira, E. F. R., Gober, M., Yarowsky, P. J., Aurelian, L. (2002). The Herpes Simplex Virus Type 2 R1 Protein Kinase (ICP10 PK) Blocks Apoptosis in Hippocampal Neurons, Involving Activation of the MEK/MAPK Survival Pathway. J. Virol. 76: 1435-1449 [Abstract] [Full Text]
Stefanis, L., Larsen, K. E., Rideout, H. J., Sulzer, D., Greene, L. A. (2001). Expression of A53T Mutant But Not Wild-Type alpha -Synuclein in PC12 Cells Induces Alterations of the Ubiquitin-Dependent Degradation System, Loss of Dopamine Release, and Autophagic Cell Death. J. Neurosci. 21: 9549-9560 [Abstract] [Full Text]
Beery, R., Haimsohn, M., Wertheim, N., Hemi, R., Nir, U., Karasik, A., Kanety, H., Geier, A. (2001). Activation of the Insulin-Like Growth Factor 1 Signaling Pathway by the Antiapoptotic Agents Aurintricarboxylic Acid and Evans Blue. Endocrinology 142: 3098-3107 [Abstract] [Full Text]
Niikura, T., Hashimoto, Y., Okamoto, T., Abe, Y., Yasukawa, T., Kawasumi, M., Hiraki, T., Kita, Y., Terashita, K., Kouyama, K., Nishimoto, I. (2001). Insulin-Like Growth Factor I (IGF-I) Protects Cells from Apoptosis by Alzheimer's V642I Mutant Amyloid Precursor Protein through IGF-I Receptor in an IGF-Binding Protein-Sensitive Manner. J. Neurosci. 21: 1902-1910 [Abstract] [Full Text]
Tessitore, L. (2000). Apoptosis and Cell Proliferation Are Involved in the Initiation of Liver Carcinogenesis by a Subnecrogenic Dose of Diethylnitrosamine in Refed Rats. J. Nutr. 130: 104-110 [Abstract] [Full Text]
Davis, P. K., Johnson, G. V. W. (1999). The Microtubule Binding of Tau and High Molecular Weight Tau in Apoptotic PC12 Cells Is Impaired because of Altered Phosphorylation. J. Biol. Chem. 274: 35686-35692 [Abstract] [Full Text]
Saragovi, H. U., Zheng, W., Maliartchouk, S., DiGugliemo, G. M., Mawal, Y. R., Kamen, A., Woo, S. B., Cuello, A. C., Debeir, T., Neet, K. E. (1998). A TrkA-selective, Fast Internalizing Nerve Growth Factor-Antibody Complex Induces Trophic but Not Neuritogenic Signals. J. Biol. Chem. 273: 34933-34940 [Abstract] [Full Text]
Efanova, I. B., Zaitsev, S. V., Zhivotovsky, B., Kohler, M., Orrenius, S., Berggren, P.-O. (1998). Glucose and Tolbutamide Induce Apoptosis in Pancreatic beta -Cells. A PROCESS DEPENDENT ON INTRACELLULAR Ca2+ CONCENTRATION. J. Biol. Chem. 273: 33501-33507 [Abstract] [Full Text]
Zaccagnini, G., Maione, B., Lorenzini, R., Spadafora, C. (1998). Increased Production of Mouse Embryos in In Vitro Fertilization by Preincubating Sperm Cells with the Nuclease Inhibitor Aurintricarboxylic Acid. Biol. Reprod. 59: 1549-1553 [Abstract] [Full Text]
Stefanis, L., Troy, C. M., Qi, H., Shelanski, M. L., Greene, L. A. (1998). Caspase-2 (Nedd-2) Processing and Death of Trophic Factor-Deprived PC12 Cells and Sympathetic Neurons Occur Independently of Caspase-3 (CPP32)-Like Activity. J. Neurosci. 18: 9204-9215 [Abstract] [Full Text]
Li, G., Segu, V. B. G., Rabaglia, M. E., Luo, R.-H., Kowluru, A., Metz, S. A. (1998). Prolonged Depletion of Guanosine Triphosphate Induces Death of Insulin-Secreting Cells by Apoptosis. Endocrinology 139: 3752-3762 [Abstract] [Full Text]
Farinelli, S. E., Greene, L. A., Friedman, W. J. (1998). Neuroprotective Actions of Dipyridamole on Cultured CNS Neurons. J. Neurosci. 18: 5112-5123 [Abstract] [Full Text]
Li, Y., Maher, P., Schubert, D. (1998). Phosphatidylcholine-specific phospholipase C regulates glutamate-induced nerve cell death. Proc. Natl. Acad. Sci. USA 95: 7748-7753 [Abstract] [Full Text]
Aronica, E. M., Gorter, J. A., Grooms, S., Kessler, J. A., Bennett, M. V.�L., Zukin, R. S., Rosenbaum, D. M. (1998). Aurintricarboxylic acid prevents GLUR2 mRNA down-regulation and delayed neurodegeneration in hippocampal CA1 neurons of gerbil after global ischemia. Proc. Natl. Acad. Sci. USA 95: 7115-7120 [Abstract] [Full Text]
Yan, C. Y. I., Greene, L. A. (1998). Prevention of PC12 Cell Death by N-Acetylcysteine Requires Activation of the Ras Pathway. J. Neurosci. 18: 4042-4049 [Abstract] [Full Text]
Mizuno, N., Yoshitomi, H., Ishida, H., Kuromi, H., Kawaki, J., Seino, Y., Seino, S. (1998). Altered bcl-2 and bax Expression and Intracellular Ca2+ Signaling in Apoptosis of Pancreatic Cells and the Impairment of Glucose-Induced Insulin Secretion. Endocrinology 139: 1429-1439 [Abstract] [Full Text]
Sawa, A., Khan, A. A., Hester, L. D., Snyder, S. H. (1997). Glyceraldehyde-3-phosphate dehydrogenase: Nuclear translocation participates in neuronal and nonneuronal cell�death. Proc. Natl. Acad. Sci. USA 94: 11669-11674 [Abstract] [Full Text]
Edsall, L. C., Pirianov, G. G., Spiegel, S. (1997). Involvement of Sphingosine 1-Phosphate in Nerve Growth Factor-Mediated Neuronal Survival and Differentiation. J. Neurosci. 17: 6952-6960 [Abstract] [Full Text]
Kruman, I., Bruce-Keller, A. J., Bredesen, D., Waeg, G., Mattson, M. P. (1997). Evidence that 4-Hydroxynonenal Mediates Oxidative Stress-Induced Neuronal Apoptosis. J. Neurosci. 17: 5089-5100 [Abstract] [Full Text]
Guo, Q., Sopher, B. L., Furukawa, K., Pham, D. G., Robinson, N., Martin, G. M., Mattson, M. P. (1997). Alzheimer's Presenilin Mutation Sensitizes Neural Cells to Apoptosis Induced by Trophic Factor Withdrawal and Amyloid beta -Peptide: Involvement of Calcium and Oxyradicals. J. Neurosci. 17: 4212-4222 [Abstract] [Full Text]
Troy, C. M., Stefanis, L., Greene, L. A., Shelanski, M. L. (1997). Nedd2 Is Required for Apoptosis after Trophic Factor Withdrawal, But Not Superoxide Dismutase (SOD1) Downregulation, in Sympathetic Neurons and PC12 Cells. J. Neurosci. 17: 1911-1918 [Abstract] [Full Text]
D'Mello, S. R., Borodezt, K., Soltoff, S. P. (1997). Insulin-Like Growth Factor and Potassium Depolarization Maintain Neuronal Survival by Distinct Pathways: Possible Involvement of PI 3-Kinase in IGF-1 Signaling. J. Neurosci. 17: 1548-1560 [Abstract] [Full Text]
Escargueil-Blanc, I., Meilhac, O., Pieraggi, M.-T., Arnal, J.-F., Salvayre, R., Negre-Salvayre, A. (1997). Oxidized LDLs Induce Massive Apoptosis of Cultured Human Endothelial Cells Through a Calcium-Dependent Pathway: Prevention by Aurintricarboxylic Acid. Arterioscler. Thromb. Vasc. Bio. 17: 331-339 [Abstract] [Full Text]
Stefanis, L., Park, D. S., Yan, C. Y. I., Farinelli, S. E., Troy, C. M., Shelanski, M. L., Greene, L. A. (1996). Induction of CPP32-like Activity in PC12 Cells by Withdrawal of Trophic Support. DISSOCIATION FROM APOPTOSIS. J. Biol. Chem. 271: 30663-30671 [Abstract] [Full Text]
Park, D. S., Stefanis, L., Yan, C. Y. I., Farinelli, S. E., Greene, L. A. (1996). Ordering the Cell Death Pathway. DIFFERENTIAL EFFECTS OF BCL2, AN INTERLEUKIN-1-CONVERTING ENZYME FAMILY PROTEASE INHIBITOR, AND OTHER SURVIVAL AGENTS ON JNK ACTIVATION IN SERUM/NERVE GROWTH FACTOR-DEPRIVED PC12 CELLS. J. Biol. Chem. 271: 21898-21905 [Abstract] [Full Text]
Bredesen, D. E. (1996). REVIEW {blacksquare} : Keeping Neurons Alive: The Molecular Control of Apoptosis (Part I. Neuroscientist 2: 181-190 [Abstract]
Park, D. S., Farinelli, S. E., Greene, L. A. (1996). Inhibitors of Cyclin-dependent Kinases Promote Survival of Post-mitotic Neuronally Differentiated PC12 Cells and Sympathetic Neurons. J. Biol. Chem. 271: 8161-8169 [Abstract] [Full Text]
Xia, Z., Dickens, M., Raingeaud, J.�l, Davis, R. J., Greenberg, M. E. (1995). Opposing Effects of ERK and JNK-p38 MAP Kinases on Apoptosis. Science 270: 1326-1331 [Abstract]
Yan, C. Y. I., Ferrari, G., Greene, L. A. (1995). N-Acetylcysteine-promoted Survival of PC12 Cells Is Glutathione-independent but Transcription-dependent. J. Biol. Chem. 270: 26827-26832 [Abstract] [Full Text]
Okada, N., Koizumi, S. (1995). A Neuroprotective Compound, Aurin Tricarboxylic Acid, Stimulates the Tyrosine Phosphorylation Cascade in PC12 Cells. J. Biol. Chem. 270: 16464-16469 [Abstract] [Full Text]
Ferrari, G., Anderson, B. L., Stephens, R. M., Kaplan, D. R., Greene, L. A. (1995). Prevention of Apoptotic Neuronal Death by G[IMAGE] Ganglioside. J. Biol. Chem. 270: 3074-3080 [Abstract] [Full Text]
Birren, S. J., Lo, L., Anderson, D. J. (1993). Sympathetic neuroblasts undergo a developmental switch in trophic dependence. Development 119: 597-610 [Abstract]
Garcia, I, Martinou, I, Tsujimoto, Y, Martinou, J. (1992). Prevention of programmed cell death of sympathetic neurons by the bcl-2 proto-oncogene. Science 258: 302-304 [Abstract]