A sialoglycoprotein complex linked to the microvillus cytoskeleton acts as a receptor for pilus (AF/R1) mediated adhesion of enteropathogenic Escherichia coli (RDEC-1) in rabbit small intestine

doi:10.1083/jcb.115.4.1021

This Article

	Full Text (PDF, 1175K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Rafiee, P.
	Articles by Kim, Y. S.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Rafiee, P.
	Articles by Kim, Y. S.


Social Bookmarking

	What's this?

ARTICLES

A sialoglycoprotein complex linked to the microvillus cytoskeleton acts as a receptor for pilus (AF/R1) mediated adhesion of enteropathogenic Escherichia coli (RDEC-1) in rabbit small intestine

P Rafiee, H Leffler, JC Byrd, FJ Cassels, EC Boedeker and YS Kim
Gastrointestinal Research Laboratory, Veterans Administration Medical Center, San Francisco, California 94121.

Escherichia coli strain RDEC-1 is an enteroadherent, diarrheagenic pathogen in rabbits that utilizes AF/R1 pili for initial (stage 1) adherence, but the host receptors for this adhesion are unknown. Here we demonstrate that RDEC-1 binds, via AF/R1 pili, to a specific rabbit ileal microvillus membrane glycoprotein receptor complex of subunits 130 and 140 kD. The binding involves sialic acid present on oligosaccharide moieties of the glycoprotein receptor. Furthermore, the microvillus membrane glycoprotein receptor complex appears to be associated with cytoskeletal components via brush border myosin 1. This newly described link between AF/R1 receptor and cytoskeletal components suggests that, in addition to this function in mucosal adherence, the pili may facilitate subsequent (second stage) close effacing attachment of RDEC-1 to the host epithelium by influencing cytoskeletal function.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Gallois, M., Gidenne, T., Tasca, C., Caubet, C., Coudert, C., Milon, A., Boullier, S. (2007). Maternal Milk Contains Antimicrobial Factors That Protect Young Rabbits from Enteropathogenic Escherichia coli Infection. CVI 14: 585-592 [Abstract] [Full Text]
Agin, T. S., Zhu, C., Johnson, L. A., Thate, T. E., Yang, Z., Boedeker, E. C. (2005). Protection against Hemorrhagic Colitis in an Animal Model by Oral Immunization with Isogeneic Rabbit Enteropathogenic Escherichia coli Attenuated by Truncating Intimin. Infect. Immun. 73: 6608-6619 [Abstract] [Full Text]
Ryu, H., Kim, Y. S., Grange, P. A., Cassels, F. J. (2001). Escherichia coli Strain RDEC-1 AF/R1 Endogenous Fimbrial Glycoconjugate Receptor Molecules in Rabbit Small Intestine. Infect. Immun. 69: 640-649 [Abstract] [Full Text]
Mantis, N. J., Frey, A., Neutra, M. R. (2000). Accessibility of glycolipid and oligosaccharide epitopes on rabbit villus and follicle-associated epithelium. Am. J. Physiol. Gastrointest. Liver Physiol. 278: G915-G923 [Abstract] [Full Text]
Ismaili, A., Meddings, J. B., Ratnam, S., Sherman, P. M. (1999). Modulation of host cell membrane fluidity: a novel mechanism for preventing bacterial adhesion. Am. J. Physiol. Gastrointest. Liver Physiol. 277: G201-G208 [Abstract] [Full Text]
Buret, A., Olson, M. E., Gall, D. G., Hardin, J. A. (1998). Effects of Orally Administered Epidermal Growth Factor on Enteropathogenic Escherichia coli Infection in Rabbits. Infect. Immun. 66: 4917-4923 [Abstract] [Full Text]