Immunohistochemical localization of TGF beta 1, TGF beta 2, and TGF beta 3 in the mouse embryo: expression patterns suggest multiple roles during embryonic development

doi:10.1083/jcb.115.4.1091

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Immunohistochemical localization of TGF beta 1, TGF beta 2, and TGF beta 3 in the mouse embryo: expression patterns suggest multiple roles during embryonic development

RW Pelton, B Saxena, M Jones, HL Moses and LI Gold
Department of Cell Biology, Vanderbilt University Medical School, Nashville, Tennessee 37232.

Isoform-specific antibodies to TGF beta 1, TGF beta 2, and TGF beta 3 proteins were generated and have been used to examine the expression of these factors in the developing mouse embryo from 12.5-18.5 d post coitum (d.p.c.). These studies demonstrate the initial characterization of both TGF beta 2 and beta 3 in mammalian embryogenesis and are compared with TGF beta 1. Expression of one or all three TGF beta proteins was observed in many tissues, e.g., cartilage, bone, teeth, muscle, heart, blood vessels, lung, kidney, gut, liver, eye, ear, skin, and nervous tissue. Furthermore, all three TGF beta proteins demonstrated discrete cell-specific patterns of expression at various stages of development and the wide variety of tissues expressing TGF beta proteins represent all three primary embryonic germ layers. For example, specific localization of TGF beta 1 was observed in the lens fibers of the eye (ectoderm), TGF beta 2 in the cortex of the adrenal gland (mesoderm), and TGF beta 3 in the cochlear epithelium of the inner ear (endoderm). Compared to the expression of TGF beta mRNA transcripts in a given embryonic tissue, TGF beta proteins were frequently colocalized within the same cell type as the mRNA, but in some cases were observed to localize to different cells than the mRNA, thereby indicating that a complex pattern of transcription, translation, and secretion for TGF beta s 1-3 exists in the mouse embryo. This also indicates that TGF beta 1, beta 2, and beta 3 act through both paracrine and autocrine mechanisms during mammalian embryogenesis.
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Laping, N. J., Everitt, J. I., Frazier, K. S., Burgert, M., Portis, M. J., Cadacio, C., Gold, L. I., Walker, C. L. (2007). Tumor-Specific Efficacy of Transforming Growth Factor-{beta}RI Inhibition in Eker Rats. Clin. Cancer Res. 13: 3087-3099 [Abstract] [Full Text]
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Janssens, K, Vanhoenacker, F, Bonduelle, M, Verbruggen, L, Van Maldergem, L, Ralston, S, Guanabens, N, Migone, N, Wientroub, S, Divizia, M T, Bergmann, C, Bennett, C, Simsek, S, Melancon, S, Cundy, T, Van Hul, W (2006). Camurati-Engelmann disease: review of the clinical, radiological, and molecular data of 24 families and implications for diagnosis and treatment. J. Med. Genet. 43: 1-11 [Abstract] [Full Text]
Wang, J., Xu, N., Feng, X., Hou, N., Zhang, J., Cheng, X., Chen, Y., Zhang, Y., Yang, X. (2005). Targeted Disruption of Smad4 in Cardiomyocytes Results in Cardiac Hypertrophy and Heart Failure. Circ. Res. 97: 821-828 [Abstract] [Full Text]
Janssens, K., ten Dijke, P., Janssens, S., Van Hul, W. (2005). Transforming Growth Factor-{beta}1 to the Bone. Endocr. Rev. 26: 743-774 [Abstract] [Full Text]
O'Rear, L, Longobardi, L, Torello, M, Law, B K, Moses, H L, Chiarelli, F, Spagnoli, A (2005). Signaling cross-talk between IGF-binding protein-3 and transforming growth factor-{beta} in mesenchymal chondroprogenitor cell growth. J Mol Endocrinol 34: 723-737 [Abstract] [Full Text]
Greene, R. M, Pisano, M M. (2005). Recent advances in understanding transforming growth factor {beta} regulation of orofacial development. Hum Exp Toxicol 24: 1-12 [Abstract]
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Oxburgh, L., Chu, G. C., Michael, S. K., Robertson, E. J. (2004). TGF{beta} superfamily signals are required for morphogenesis of the kidney mesenchyme progenitor population. Development 131: 4593-4605 [Abstract] [Full Text]
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McLennan, I. S., Koishi, K. (2004). Fetal and Maternal Transforming Growth Factor-{beta}1 May Combine to Maintain Pregnancy in Mice. Biol. Reprod. 70: 1614-1618 [Abstract] [Full Text]
Saika, S., Okada, Y., Miyamoto, T., Yamanaka, O., Ohnishi, Y., Ooshima, A., Liu, C.-Y., Weng, D., Kao, W. W.-Y. (2004). Role of p38 MAP Kinase in Regulation of Cell Migration and Proliferation in Healing Corneal Epithelium. IOVS 45: 100-109 [Abstract] [Full Text]
Soo, C., Beanes, S. R., Hu, F.-Y., Zhang, X., Dang, C., Chang, G., Wang, Y., Nishimura, I., Freymiller, E., Longaker, M. T., Lorenz, H.P., Ting, K. (2003). Ontogenetic Transition in Fetal Wound Transforming Growth Factor-{beta} Regulation Correlates with Collagen Organization. Am. J. Pathol. 163: 2459-2476 [Abstract] [Full Text]
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Cheng, J., Grande, J. P. (2002). Transforming Growth Factor-{beta} Signal Transduction and Progressive Renal Disease. Exp. Biol. Med. 227: 943-956 [Abstract] [Full Text]
Vicencio, A. G., Eickelberg, O., Stankewich, M. C., Kashgarian, M., Haddad, G. G. (2002). Regulation of TGF-beta ligand and receptor expression in neonatal rat lungs exposed to chronic hypoxia. J. Appl. Physiol. 93: 1123-1130 [Abstract] [Full Text]
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Ito, Y., Zhao, J., Mogharei, A., Shuler, C. F., Weinstein, M., Deng, C., Chai, Y. (2001). Antagonistic Effects of Smad2 Versus Smad7 Are Sensitive to Their Expression Level during Tooth Development. J. Biol. Chem. 276: 44163-44172 [Abstract] [Full Text]
Sloan, A.J., Couble, M.-L., Bleicher, F., Magloire, H., Smith, A.J., Farges, J.-C. (2001). Expression of TGF-{beta} Receptors I and II in the Human Dental Pulp by in situ Hybridization. ADR 15: 63-67 [Abstract]
Sheen-Chen, S.-M., Chen, H.-S., Sheen, C.-W., Eng, H.-L., Chen, W.-J. (2001). Serum Levels of Transforming Growth Factor {beta}1 in Patients With Breast Cancer. Arch Surg 136: 937-940 [Abstract] [Full Text]
ITO, Y., GOLDSCHMEDING, R., BENDE, R. J., CLAESSEN, N., CHAND, M. A., KLEIJ, L., RABELINK, T. J., WEENING, J. J., ATEN, J. (2001). Kinetics of Connective Tissue Growth Factor Expression during Experimental Proliferative Glomerulonephritis. J. Am. Soc. Nephrol. 12: 472-484 [Abstract] [Full Text]
Obermuller, N., Morente, N., Kranzlin, B., Gretz, N., Witzgall, R. (2001). A possible role for metalloproteinases in renal cyst development. Am. J. Physiol. Renal Physiol. 280: F540-F550 [Abstract] [Full Text]
Plisov, S., Yoshino, K, Dove, L., Higinbotham, K., Rubin, J., Perantoni, A. (2001). TGF beta 2, LIF and FGF2 cooperate to induce nephrogenesis. Development 128: 1045-1057 [Abstract]
Yang, S. P., Woolf, A. S., Yuan, H. T., Scott, R. J., Risdon, R. A., O'Hare, M. J., Winyard, P. J. D. (2000). Potential Biological Role of Transforming Growth Factor-{beta}1 in Human Congenital Kidney Malformations. Am. J. Pathol. 157: 1633-1647 [Abstract] [Full Text]
Dou, C., Lee, J., Liu, B., Liu, F., Massague, J., Xuan, S., Lai, E. (2000). BF-1 Interferes with Transforming Growth Factor beta Signaling by Associating with Smad Partners. Mol. Cell. Biol. 20: 6201-6211 [Abstract] [Full Text]
Melin, M., Joffre-Romeas, A., Farges, J.-C., Couble, M.-L., Magloire, H., Bleicher, F. (2000). Effects of TGF{beta} 1 on Dental Pulp Cells in Cultured Human Tooth Slices. JDR 79: 1689-1696 [Abstract]
Damon, D. H. (2000). VSM growth is stimulated in sympathetic neuron/VSM cocultures: role of TGF-beta 2 and endothelin. Am. J. Physiol. Heart Circ. Physiol. 278: H404-H411 [Abstract] [Full Text]
Fuller, K, Lean, J., Bayley, K., Wani, M., Chambers, T. (2000). A role for TGFbeta(1) in osteoclast differentiation and survival. J. Cell Sci. 113: 2445-2453 [Abstract]
Ireland, M. E., Mrock, L. K. (2000). Differentiation of Chick Lens Epithelial Cells: Involvement of the Epidermal Growth Factor Receptor and Endogenous Ligand. IOVS 41: 183-190 [Abstract] [Full Text]
Young, D.L., Schneider, R.A., Hu, D., Helms, J.A. (2000). Genetic and Teratogenic Approaches To Craniofacial Development. CROBM 11: 304-317 [Abstract] [Full Text]
Yan, Q., Sage, E. H. (1999). SPARC, a Matricellular Glycoprotein with Important Biological Functions. J. Histochem. Cytochem. 47: 1495-1506 [Full Text]
Hollnagel, A., Oehlmann, V., Heymer, J., Ruther, U., Nordheim, A. (1999). Id Genes Are Direct Targets of Bone Morphogenetic Protein Induction in Embryonic Stem Cells. J. Biol. Chem. 274: 19838-19845 [Abstract] [Full Text]
Filvaroff, E, Erlebacher, A, Ye, J, Gitelman, S., Lotz, J, Heillman, M, Derynck, R (1999). Inhibition of TGF-beta receptor signaling in osteoblasts leads to decreased bone remodeling and increased trabecular bone mass. Development 126: 4267-4279 [Abstract]
Deng, X., Bellis, S., Yan, Z., Friedman, E. (1999). Differential Responsiveness to Autocrine and Exogenous Transforming Growth Factor (TGF) {beta}1 in Cells with Nonfunctional TGF-{beta} Receptor Type III. Cell Growth Differ. 10: 11-18 [Abstract] [Full Text]
Montuenga, L. M., Mariano, J. M., Prentice, M. A., Cuttitta, F., Jakowlew, S. B. (1998). Coordinate Expression of Transforming Growth Factor-{beta}1 and Adrenomedullin in Rodent Embryogenesis. Endocrinology 139: 3946-3957 [Abstract] [Full Text]
Kelly, D., Kim, S.-J., Rizzino, A. (1998). Transcriptional Activation of the Type II Transforming Growth Factor-beta Receptor Gene upon Differentiation of Embryonal Carcinoma Cells. J. Biol. Chem. 273: 21115-21124 [Abstract] [Full Text]
Sahlberg, C., Hormia, M., Airenne, T., Thesleffl, I. (1998). Laminin y2 Expression is Developmentally Regulated during Murine Tooth Morphogenesis and is Intense in Ameloblasts. JDR 77: 1589-1596 [Abstract]
Erlebacher, A., Filvaroff, E. H., Ye, J.-Q., Derynck, R. (1998). Osteoblastic Responses to TGF-beta during Bone Remodeling. Mol. Biol. Cell 9: 1903-1918 [Abstract] [Full Text]
Sun, D, Vanderburg, C., Odierna, G., Hay, E. (1998). TGFbeta3 promotes transformation of chicken palate medial edge epithelium to mesenchyme in vitro. Development 125: 95-105 [Abstract]
Serra, R., Johnson, M., Filvaroff, E. H., LaBorde, J., Sheehan, D. M., Derynck, R., Moses, H. L. (1997). Expression of a Truncated, Kinase-Defective TGF-{beta} Type II Receptor in Mouse Skeletal Tissue Promotes Terminal Chondrocyte Differentiation and Osteoarthritis. JCB 139: 541-552 [Abstract] [Full Text]
Lyon, M., Rushton, G., Gallagher, J. T. (1997). The Interaction of the Transforming Growth Factor-beta s with Heparin/Heparan Sulfate Is Isoform-specific. J. Biol. Chem. 272: 18000-18006 [Abstract] [Full Text]
Dobbertin, A., Schmid, P., Gelman, M., Glowinski, J., Mallat, M. (1997). Neurons Promote Macrophage Proliferation by Producing Transforming Growth Factor-beta 2. J. Neurosci. 17: 5305-5315 [Abstract] [Full Text]
McGowan, S. E., Jackson, S. K., Olson, P. J., Parekh, T., Gold, L. I. (1997). Exogenous and Endogenous Transforming Growth Factors-beta Influence Elastin Gene Expression in Cultured Lung Fibroblasts. Am. J. Respir. Cell Mol. Bio. 17: 25-35 [Abstract] [Full Text]
Wu, L., Siddiqui, A., Morris, D. E., Cox, D. A., Roth, S. I., Mustoe, T. A. (1997). Transforming Growth Factor {beta}3 (TGF{beta}3) Accelerates Wound Healing Without Alteration of Scar Prominence: Histologic and Competitive Reverse-Transcription-Polymerase Chain Reaction Studies. Arch Surg 132: 753-760 [Abstract]
Hales, A. M., Chamberlain, C. G., Murphy, C. R., McAvoy, J. W. (1997). Estrogen Protects Lenses against Cataract Induced by Transforming Growth Factor-{beta} (TGF{beta}). JEM 185: 273-280 [Abstract] [Full Text]
Stern, H., Lin-Jones, J, Hauschka, S. (1997). Synergistic interactions between bFGF and a TGF-beta family member may mediate myogenic signals from the neural tube. Development 124: 3511-3523 [Abstract]
Sanford, L., Ormsby, I, Gittenberger-de Groot, A., Sariola, H, Friedman, R, Boivin, G., Cardell, E., Doetschman, T (1997). TGFbeta2 knockout mice have multiple developmental defects that are non-overlapping with other TGFbeta knockout phenotypes. Development 124: 2659-2670 [Abstract]
Maas, R., Bei, M. (1997). The Genetic Control of Early Tooth Development. CROBM 8: 4-39 [Abstract] [Full Text]
Montuenga, L. M., Martinez, A., Miller, M. J., Unsworth, E. J., Cuttitta, F. (1997). Expression of Adrenomedullin and Its Receptor during Embryogenesis Suggests Autocrine or Paracrine Modes of Action. Endocrinology 138: 440-451 [Abstract] [Full Text]
Ryden, M., Imamura, T., Jornvall, H., Belluardo, N., Neveu, I., Trupp, M., Okadome, T., ten Dijke, P., Ibanez, C. F. (1996). A Novel Type I Receptor Serine-Threonine Kinase Predominantly Expressed in the Adult Central Nervous System. J. Biol. Chem. 271: 30603-30609 [Abstract] [Full Text]
Taipale, J., Keski-Oja, J. (1996). Hepatocyte Growth Factor Releases Epithelial and Endothelial Cells from Growth Arrest Induced by Transforming Growth Factor-beta1. J. Biol. Chem. 271: 4342-4348 [Abstract] [Full Text]
Bellusci, S, Henderson, R, Winnier, G, Oikawa, T, Hogan, B. (1996). Evidence from normal expression and targeted misexpression that bone morphogenetic protein (Bmp-4) plays a role in mouse embryonic lung morphogenesis. Development 122: 1693-1702 [Abstract]
Taipale, J., Lohi, J., Saarinen, J., Kovanen, P. T., Keski-Oja, J. (1995). Human Mast Cell Chymase and Leukocyte Elastase Release Latent Transforming Growth Factor-beta1 from the Extracellular Matrix of Cultured Human Epithelial and Endothelial Cells. J. Biol. Chem. 270: 4689-4696 [Abstract] [Full Text]
Glick, A B, Lee, M M, Darwiche, N, Kulkarni, A B, Karlsson, S, Yuspa, S H (1994). Targeted deletion of the TGF-beta 1 gene causes rapid progression to squamous cell carcinoma.. Genes Dev. 8: 2429-2440 [Abstract]
Heikinheimo, K. (1994). Stage-specific Expression of Decapentaplegic-Vg-related Genes 2, 4, and 6 (Bone Morphogenetic Proteins 2, 4, and 6) During Human Tooth Morphogenesis. JDR 73: 590-597 [Abstract]
Buchman, V., Sporn, M, Davies, A. (1994). Role of transforming growth factor-beta isoforms in regulating the expression of nerve growth factor and neurotrophin-3 mRNA levels in embryonic cutaneous cells at different stages of development. Development 120: 1621-1629 [Abstract]
Filvaroff, E., Ebner, R, Derynck, R (1994). Inhibition of myogenic differentiation in myoblasts expressing a truncated type II TGF-beta receptor. Development 120: 1085-1095 [Abstract]
Lawler, S, Candia, A., Ebner, R, Shum, L, Lopez, A., Moses, H., Wright, C., Derynck, R (1994). The murine type II TGF-beta receptor has a coincident embryonic expression and binding preference for TGF-beta 1. Development 120: 165-175 [Abstract]
Nieto, M., Bennett, M., Sargent, M., Wilkinson, D. (1992). Cloning and developmental expression of Sna, a murine homologue of the Drosophila snail gene. Development 116: 227-237 [Abstract]
Blauer, M, Kohonen, J, Leivonen, I, Vilja, P, Tuohimaa, P (1992). Inhibin alpha- and beta A-subunit immunoreactivity in the chicken embryo during morphogenesis. Development 115: 1079-1086 [Abstract]