Slow calcium waves accompany cytokinesis in medaka fish eggs

doi:10.1083/jcb.115.5.1259

Quantitative Colocalization Analysis Software

This Article

	Full Text (PDF, 894K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Fluck, R. A.
	Articles by Jaffe, L. F.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Fluck, R. A.
	Articles by Jaffe, L. F.


Social Bookmarking

	What's this?

ARTICLES

Slow calcium waves accompany cytokinesis in medaka fish eggs

RA Fluck, AL Miller and LF Jaffe
Marine Biological Laboratory, Woods Hole, Massachusetts 02543.

Animal cells are cleaved by the formation and contraction of an extremely thin actomyosin band. In most cases this contractile band seems to form synchronously around the whole equator of the cleaving cell; however in giant cells it first forms near the mitotic apparatus and then slowly grows outwards over the cell. We studied the relationship of calcium to such contractile band growth using aequorin injected medaka fish eggs: we see two successive waves of faint luminescence moving along each of the first three cleavage furrows at approximately 0.5 micron/s. The first, narrower waves accompany furrow extension, while the second, broader ones, accompany the subsequent apposition or slow zipping together of the separating cells. If the first waves travel within the assembling contractile band, they would indicate local increases of free calcium to concentrations of about five to eight micromolar. This is the first report to visualize high free calcium within cleavage furrows. Moreover, this is also the first report to visualize slow (0.3-1.0 micron/s) as opposed to fast (10-100 microns/s) calcium waves. We suggest that these first waves are needed for furrow growth; that in part they further furrow growth by speeding actomyosin filament shortening, while such shortening in turn acts to mechanically release calcium and thus propagates these waves as well as furrow growth. We also suggest that the second waves act to induce the exocytosis which provides new furrow membrane.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Jaffe, L. F (2008). Calcium waves. Phil Trans R Soc B 363: 1311-1317 [Abstract] [Full Text]
Webb, S. E, Li, W. M., Miller, A. L (2008). Calcium signalling during the cleavage period of zebrafish development. Phil Trans R Soc B 363: 1363-1369 [Abstract] [Full Text]
Whitaker, M. (2008). Calcium signalling in early embryos. Phil Trans R Soc B 363: 1401-1418 [Abstract] [Full Text]
Lucero, A., Stack, C., Bresnick, A. R., Shuster, C. B. (2006). A Global, Myosin Light Chain Kinase-dependent Increase in Myosin II Contractility Accompanies the Metaphase-Anaphase Transition in Sea Urchin Eggs. Mol. Biol. Cell 17: 4093-4104 [Abstract] [Full Text]
Whitaker, M. (2006). Calcium at Fertilization and in Early Development. Physiol. Rev. 86: 25-88 [Abstract] [Full Text]
Parry, H., McDougall, A., Whitaker, M. (2005). Microdomains bounded by endoplasmic reticulum segregate cell cycle calcium transients in syncytial Drosophila embryos. JCB 171: 47-59 [Abstract] [Full Text]
FitzHarris, G., Larman, M., Richards, C., Carroll, J. (2005). An increase in [Ca2+]i is sufficient but not necessary for driving mitosis in early mouse embryos. J. Cell Sci. 118: 4563-4575 [Abstract] [Full Text]
Saul, D., Fabian, L., Forer, A., Brill, J. A. (2004). Continuous phosphatidylinositol metabolism is required for cleavage of crane fly spermatocytes. J. Cell Sci. 117: 3887-3896 [Abstract] [Full Text]
Noguchi, T., Mabuchi, I. (2002). Localized Calcium Signals along the Cleavage Furrow of the Xenopus Egg Are Not Involved in Cytokinesis. Mol. Biol. Cell 13: 1263-1273 [Abstract] [Full Text]
Antoine, A. F., Faure, J.-E., Cordeiro, S., Dumas, C., Rougier, M., Feijó, J. A. (2000). A calcium influx is triggered and propagates in the zygote as a wavefront during in vitro fertilization of flowering plants. Proc. Natl. Acad. Sci. USA 10.1073/pnas.180243697v1 [Abstract] [Full Text]
Jesuthasan, S (1999). Furrow-associated microtubule arrays are required for the cohesion of zebrafish blastomeres following cytokinesis. J. Cell Sci. 111: 3695-3703 [Abstract]
Zhao, M., Forrester, J. V., McCaig, C. D. (1999). A small, physiological electric field orients cell division. Proc. Natl. Acad. Sci. USA 96: 4942-4946 [Abstract] [Full Text]
Beckhelling, C, Penny, C, Clyde, S, Ford, C (1999). Timing of calcium and protein synthesis requirements for the first mitotic cell cycle in fertilised Xenopus eggs. J. Cell Sci. 112: 3975-3984 [Abstract]
Matsumura, F., Ono, S., Yamakita, Y., Totsukawa, G., Yamashiro, S. (1998). Specific Localization of Serine 19 Phosphorylated Myosin II during Cell Locomotion and Mitosis of Cultured Cells. JCB 140: 119-129 [Abstract] [Full Text]
Creton, R, Speksnijder, J., Jaffe, L. (1998). Patterns of free calcium in zebrafish embryos. J. Cell Sci. 111: 1613-1622 [Abstract]
Roegiers, F, McDougall, A, Sardet, C (1995). The sperm entry point defines the orientation of the calcium-induced contraction wave that directs the first phase of cytoplasmic reorganization in the ascidian egg. Development 121: 3457-3466 [Abstract]
Miller, A., Fluck, R., McLaughlin, J., Jaffe, L. (1993). Calcium buffer injections inhibit cytokinesis in Xenopus eggs. J. Cell Sci. 106: 523-534 [Abstract]
Antoine, A. F., Faure, J.-E., Cordeiro, S., Dumas, C., Rougier, M., Feijo, J. A. (2000). A calcium influx is triggered and propagates in the zygote as a wavefront during in vitro fertilization of flowering plants. Proc. Natl. Acad. Sci. USA 97: 10643-10648 [Abstract] [Full Text]