Protein folding causes an arrest of preprotein translocation into mitochondria in vivo

doi:10.1083/jcb.115.6.1601

This Article

	Full Text (PDF, 1193K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Wienhues, U.
	Articles by Neupert, W.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Wienhues, U.
	Articles by Neupert, W.


Social Bookmarking

	What's this?

ARTICLES

Protein folding causes an arrest of preprotein translocation into mitochondria in vivo

U Wienhues, K Becker, M Schleyer, B Guiard, M Tropschug, AL Horwich, N Pfanner and W Neupert
Institut fur Physiologische Chemie, Universitat Munchen, Germany.

With vital yeast cells, a hybrid protein consisting of the amino- terminal third of the precursor to cytochrome b2 and of the entire dihydrofolate reductase was arrested on the import pathway into mitochondria. Accumulation of the protein in the mitochondrial membranes was achieved by inducing a stable tertiary structure of the dihydrofolate reductase domain. Thereby, three salient features of mitochondrial protein uptake in vivo were demonstrated: its posttranslational character; the requirement for unfolding of precursors; and import through translocation contact sites. The permanent occupation of translocation sites by the fusion protein inhibited the import of other precursors; it did, however, not lead to leakage of mitochondrial ions, implying the existence of a channel that is sealed around the membrane spanning polypeptide segment.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Torrado, L. C., Temmerman, K., Muller, H.-M., Mayer, M. P., Seelenmeyer, C., Backhaus, R., Nickel, W. (2009). An intrinsic quality-control mechanism ensures unconventional secretion of fibroblast growth factor 2 in a folded conformation. J. Cell Sci. 122: 3322-3329 [Abstract] [Full Text]
Wagner, K., Gebert, N., Guiard, B., Brandner, K., Truscott, K. N., Wiedemann, N., Pfanner, N., Rehling, P. (2008). The Assembly Pathway of the Mitochondrial Carrier Translocase Involves Four Preprotein Translocases. Mol. Cell. Biol. 28: 4251-4260 [Abstract] [Full Text]
Krayl, M., Lim, J. H., Martin, F., Guiard, B., Voos, W. (2007). A Cooperative Action of the ATP-Dependent Import Motor Complex and the Inner Membrane Potential Drives Mitochondrial Preprotein Import. Mol. Cell. Biol. 27: 411-425 [Abstract] [Full Text]
Ahmed, A. U., Beech, P. L., Lay, S. T., Gilson, P. R., Fisher, P. R. (2006). Import-Associated Translational Inhibition: Novel In Vivo Evidence for Cotranslational Protein Import into Dictyostelium discoideum Mitochondria. Eukaryot Cell 5: 1314-1327 [Abstract] [Full Text]
Backhaus, R., Zehe, C., Wegehingel, S., Kehlenbach, A., Schwappach, B., Nickel, W. (2004). Unconventional protein secretion: membrane translocation of FGF-2 does not require protein unfolding. J. Cell Sci. 117: 1727-1736 [Abstract] [Full Text]
Schricker, R., Angermayr, M., Strobel, G., Klinke, S., Korber, D., Bandlow, W. (2002). Redundant Mitochondrial Targeting Signals in Yeast Adenylate Kinase. J. Biol. Chem. 277: 28757-28764 [Abstract] [Full Text]
Artigues, A., Iriarte, A., Martinez-Carrion, M. (2002). Binding to Chaperones Allows Import of a Purified Mitochondrial Precursor into Mitochondria. J. Biol. Chem. 277: 25047-25055 [Abstract] [Full Text]
Huang, S., Murphy, S., Matouschek, A. (2000). Effect of the protein import machinery at the mitochondrial surface on precursor stability. Proc. Natl. Acad. Sci. USA 10.1073/pnas.230243097v1 [Abstract] [Full Text]
Funfschilling, U., Rospert, S. (1999). Nascent Polypeptide-associated Complex Stimulates Protein Import into Yeast Mitochondria. Mol. Biol. Cell 10: 3289-3299 [Abstract] [Full Text]
Schulke, N., Sepuri, N. B. V., Gordon, D. M., Saxena, S., Dancis, A., Pain, D. (1999). A Multisubunit Complex of Outer and Inner Mitochondrial Membrane Protein Translocases Stabilized in Vivo by Translocation Intermediates. J. Biol. Chem. 274: 22847-22854 [Abstract] [Full Text]
Lumb, M. J., Drake, A. F., Danpure, C. J. (1999). Effect of N-terminal alpha -Helix Formation on the Dimerization and Intracellular Targeting of Alanine:Glyoxylate Aminotransferase. J. Biol. Chem. 274: 20587-20596 [Abstract] [Full Text]
Lange, H., Kispal, G., Lill, R. (1999). Mechanism of Iron Transport to the Site of Heme Synthesis inside Yeast Mitochondria. J. Biol. Chem. 274: 18989-18996 [Abstract] [Full Text]
Ni, L., Heard, T. S., Weiner, H. (1999). In Vivo Mitochondrial Import. A COMPARISON OF LEADER SEQUENCE CHARGE AND STRUCTURAL RELATIONSHIPS WITH THE IN VITRO MODEL RESULTING IN EVIDENCE FOR CO-TRANSLATIONAL IMPORT. J. Biol. Chem. 274: 12685-12691 [Abstract] [Full Text]
Teter, S. A., Theg, S. M. (1998). Energy-transducing thylakoid membranes remain highly impermeable to ions during�protein�translocation. Proc. Natl. Acad. Sci. USA 95: 1590-1594 [Abstract] [Full Text]
Bomer, U., Meijer, M., Guiard, B., Dietmeier, K., Pfanner, N., Rassow, J. (1997). The Sorting Route of Cytochrome b2 Branches from the General Mitochondrial Import Pathway at the Preprotein Translocase of the Inner Membrane. J. Biol. Chem. 272: 30439-30446 [Abstract] [Full Text]
Schulke, N., Sepuri, N. B. V., Pain, D. (1997). In vivo zippering of inner and outer mitochondrial membranes by a stable translocation�intermediate. Proc. Natl. Acad. Sci. USA 94: 7314-7319 [Abstract] [Full Text]
Schatz, G., Schatz, G. (1996). The Protein Import System of Mitochondria. J. Biol. Chem. 271: 31763-31766 [Full Text]
Johnston, J. A., Johnson, E. S., Waller, P. R. H., Varshavsky, A. (1995). Methotrexate Inhibits Proteolysis of Dihydrofolate Reductase by the N-end Rule Pathway. J. Biol. Chem. 270: 8172-8178 [Abstract] [Full Text]
Lithgow, T., Horst, M., Rospert, S., Matouschek, A., Haucke, V., Schatz, G. (1995). Import and Folding of Proteins by Mitochondria. Cold Spring Harb Symp Quant Biol 60: 609-617 [Abstract]
Paunola, E., Qiao, M., Shmelev, A., Makarow, M. (2001). Inhibition of Translocation of beta -Lactamase into the Yeast Endoplasmic Reticulum by Covalently Bound Benzylpenicillin. J. Biol. Chem. 276: 34553-34559 [Abstract] [Full Text]
Huang, S., Murphy, S., Matouschek, A. (2000). Effect of the protein import machinery at the mitochondrial surface on precursor stability. Proc. Natl. Acad. Sci. USA 97: 12991-12996 [Abstract] [Full Text]