Perturbation of the morphology of the trans-Golgi network following Brefeldin A treatment: redistribution of a TGN-specific integral membrane protein, TGN38

doi:10.1083/jcb.116.1.85

This Article

	Full Text (PDF, 1260K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Reaves, B.
	Articles by Banting, G.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Reaves, B.
	Articles by Banting, G.


Social Bookmarking

	What's this?

ARTICLES

Perturbation of the morphology of the trans-Golgi network following Brefeldin A treatment: redistribution of a TGN-specific integral membrane protein, TGN38

B Reaves and G Banting
Department of Biochemistry, School of Medical Sciences, University of Bristol, U.K.

Brefeldin A (BFA) has a dramatic effect on the morphology of the Golgi apparatus and induces a rapid redistribution of Golgi proteins into the ER (Lippincott-Schwartz, J., L. C. Yuan, J. S. Bonifacino, and R. D. Klausner. 1989. Cell. 56:801-813). To date, no evidence that BFA affects the morphology of the trans-Golgi network (TGN) has been presented. We describe the results of experiments, using a polyclonal antiserum to a TGN specific integral membrane protein (TGN38) (Luzio, J.P., B. Brake, G. Banting, K. E. Howell, P. Braghetta, and K. K. Stanley. 1990. Biochem. J. 270:97-102), which demonstrate that incubation of cells with BFA does induce morphological changes to the TGN. However, rather than redistributing to the ER, the majority of the TGN collapses around the microtubule organizing center (MTOC). The effect of BFA upon the TGN is (a) independent of protein synthesis, (b) fully reversible (c) microtubule dependent (as shown in nocodazole- treated cells), and (d) relies upon the hydrolysis of GTP (as shown by performing experiments in the presence of GTP gamma S). ATP depletion reduces the ability of BFA to induce a redistribution of Golgi proteins into the ER; however, it has no effect upon the BFA-induced relocalizations of the TGN. These data confirm that the TGN is an organelle which is independent of the Golgi, and suggest a dynamic interaction between the TGN and microtubules which is centered around the MTOC.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

del Castillo, F. J., Cohen-Salmon, M., Charollais, A., Caille, D., Lampe, P. D., Chavrier, P., Meda, P., Petit, C. (2009). Consortin, a trans-Golgi network cargo receptor for the plasma membrane targeting and recycling of connexins. Hum Mol Genet 0: ddp490v2-ddp490 [Abstract] [Full Text]
Marie, M., Dale, H. A., Sannerud, R., Saraste, J. (2009). The Function of the Intermediate Compartment in Pre-Golgi Trafficking Involves its Stable Connection with the Centrosome. Mol. Biol. Cell 20: 4458-4470 [Abstract] [Full Text]
Bernardino, A. L. F., Myers, T. A., Alvarez, X., Hasegawa, A., Philipp, M. T. (2008). Toll-Like Receptors: Insights into Their Possible Role in the Pathogenesis of Lyme Neuroborreliosis. Infect. Immun. 76: 4385-4395 [Abstract] [Full Text]
Holloway, Z. G., Grabski, R., Szul, T., Styers, M. L., Coventry, J. A., Monaco, A. P., Sztul, E. (2007). Activation of ADP-ribosylation factor regulates biogenesis of the ATP7A-containing trans-Golgi network compartment and its Cu-induced trafficking. Am. J. Physiol. Cell Physiol. 293: C1753-C1767 [Abstract] [Full Text]
Radulescu, A. E., Siddhanta, A., Shields, D. (2007). A Role for Clathrin in Reassembly of the Golgi Apparatus. Mol. Biol. Cell 18: 94-105 [Abstract] [Full Text]
Hatanaka, T., Hatanaka, Y., Tsuchida, J.-i., Ganapathy, V., Setou, M. (2006). Amino Acid Transporter ATA2 Is Stored at the trans-Golgi Network and Released by Insulin Stimulus in Adipocytes. J. Biol. Chem. 281: 39273-39284 [Abstract] [Full Text]
Schaub, B. E., Berger, B., Berger, E. G., Rohrer, J. (2006). Transition of Galactosyltransferase 1 from Trans-Golgi Cisterna to the Trans-Golgi Network Is Signal Mediated. Mol. Biol. Cell 17: 5153-5162 [Abstract] [Full Text]
Williams, D., Hicks, S. W., Machamer, C. E., Pessin, J. E. (2006). Golgin-160 Is Required for the Golgi Membrane Sorting of the Insulin-responsive Glucose Transporter GLUT4 in Adipocytes. Mol. Biol. Cell 17: 5346-5355 [Abstract] [Full Text]
Schon, S., Prante, C., Bahr, C., Kuhn, J., Kleesiek, K., Gotting, C. (2006). Cloning and Recombinant Expression of Active Full-length Xylosyltransferase I (XT-I) and Characterization of Subcellular Localization of XT-I and XT-II. J. Biol. Chem. 281: 14224-14231 [Abstract] [Full Text]
Yoshino, A., Setty, S. R. G., Poynton, C., Whiteman, E. L., Saint-Pol, A., Burd, C. G., Johannes, L., Holzbaur, E. L., Koval, M., McCaffery, J. M., Marks, M. S. (2005). tGolgin-1 (p230, golgin-245) modulates Shiga-toxin transport to the Golgi and Golgi motility towards the microtubule-organizing centre. J. Cell Sci. 118: 2279-2293 [Abstract] [Full Text]
del Rio, T., Ch'ng, T. H., Flood, E. A., Gross, S. P., Enquist, L. W. (2005). Heterogeneity of a Fluorescent Tegument Component in Single Pseudorabies Virus Virions and Enveloped Axonal Assemblies. J. Virol. 79: 3903-3919 [Abstract] [Full Text]
Wagner, C. A., Finberg, K. E., Breton, S., Marshansky, V., Brown, D., Geibel, J. P. (2004). Renal Vacuolar H+-ATPase. Physiol. Rev. 84: 1263-1314 [Abstract] [Full Text]
Sarnataro, D., Campana, V., Paladino, S., Stornaiuolo, M., Nitsch, L., Zurzolo, C. (2004). PrPC Association with Lipid Rafts in the Early Secretory Pathway Stabilizes Its Cellular Conformation. Mol. Biol. Cell 15: 4031-4042 [Abstract] [Full Text]
Yoshimura, S.-i., Yamamoto, A., Misumi, Y., Sohda, M., Barr, F. A., Fujii, G., Shakoori, A., Ohno, H., Mihara, K., Nakamura, N. (2004). Dynamics of Golgi Matrix Proteins after the Blockage of ER to Golgi Transport. J Biochem 135: 201-216 [Abstract] [Full Text]
Spitzenberger, F., Pietropaolo, S., Verkade, P., Habermann, B., Lacas-Gervais, S., Mziaut, H., Pietropaolo, M., Solimena, M. (2003). Islet Cell Autoantigen of 69 kDa Is an Arfaptin-related Protein Associated with the Golgi Complex of Insulinoma INS-1 Cells. J. Biol. Chem. 278: 26166-26173 [Abstract] [Full Text]
Hickson, G. R.X., Matheson, J., Riggs, B., Maier, V. H., Fielding, A. B., Prekeris, R., Sullivan, W., Barr, F. A., Gould, G. W. (2003). Arfophilins Are Dual Arf/Rab 11 Binding Proteins That Regulate Recycling Endosome Distribution and Are Related to Drosophila Nuclear Fallout. Mol. Biol. Cell 14: 2908-2920 [Abstract] [Full Text]
Kim, B-E, Smith, K, Petris, M J (2003). A copper treatable Menkes disease mutation associated with defective trafficking of a functional Menkes copper ATPase. J. Med. Genet. 40: 290-295 [Full Text]
Kirschke, C. P., Huang, L. (2003). ZnT7, a Novel Mammalian Zinc Transporter, Accumulates Zinc in the Golgi Apparatus. J. Biol. Chem. 278: 4096-4102 [Abstract] [Full Text]
Taylor, S. G., Osman, N., McKenzie, I. F.C., Sandrin, M. S. (2002). Reduction of {alpha}-Gal expression by relocalizing {alpha}-galactosidase to the trans-Golgi network and cell surface. Glycobiology 12: 729-739 [Abstract] [Full Text]
Nebenfuhr, A., Ritzenthaler, C., Robinson, D. G. (2002). Brefeldin A: Deciphering an Enigmatic Inhibitor of Secretion. Plant Physiol. 130: 1102-1108 [Full Text]
Korkmaz, K. S., Elbi, C., Korkmaz, C. G., Loda, M., Hager, G. L., Saatcioglu, F. (2002). Molecular Cloning and Characterization of STAMP1, a Highly Prostate-specific Six Transmembrane Protein that Is Overexpressed in Prostate Cancer. J. Biol. Chem. 277: 36689-36696 [Abstract] [Full Text]
Ladinsky, M. S., Wu, C. C., McIntosh, S., McIntosh, J. R., Howell, K. E. (2002). Structure of the Golgi and Distribution of Reporter Molecules at 20{degrees}C Reveals the Complexity of the Exit Compartments. Mol. Biol. Cell 13: 2810-2825 [Abstract] [Full Text]
Moreno, R. D., Schatten, G., Ramalho-Santos, J. (2002). Golgi Apparatus Dynamics During Mouse Oocyte In Vitro Maturation: Effect of the Membrane Trafficking Inhibitor Brefeldin A. Biol. Reprod. 66: 1259-1266 [Abstract] [Full Text]
Yoo, J.-S., Moyer, B. D., Bannykh, S., Yoo, H.-M., Riordan, J. R., Balch, W. E. (2002). Non-conventional Trafficking of the Cystic Fibrosis Transmembrane Conductance Regulator through the Early Secretory Pathway. J. Biol. Chem. 277: 11401-11409 [Abstract] [Full Text]
Das Sarma, J., Meyer, R. A., Wang, F., Abraham, V., Lo, C. W., Koval, M. (2002). Multimeric connexin interactions prior to the trans-Golgi network. J. Cell Sci. 114: 4013-4024 [Abstract] [Full Text]
Windheim, M., Burgert, H.-G. (2002). Characterization of E3/49K, a Novel, Highly Glycosylated E3 Protein of the Epidemic Keratoconjunctivitis-Causing Adenovirus Type 19a. J. Virol. 76: 755-766 [Abstract] [Full Text]
Yeaman, C., Grindstaff, K. K., Wright, J. R., Nelson, W. J. (2001). Sec6/8 complexes on trans-Golgi network and plasma membrane regulate late stages of exocytosis in mammalian cells. JCB 155: 593-604 [Abstract] [Full Text]
Chabin-Brion, K., Marceiller, J., Perez, F., Settegrana, C., Drechou, A., Durand, G., Pous, C. (2001). The Golgi Complex Is a Microtubule-organizing Organelle. Mol. Biol. Cell 12: 2047-2060 [Abstract] [Full Text]
Granzow, H., Klupp, B. G., Fuchs, W., Veits, J., Osterrieder, N., Mettenleiter, T. C. (2001). Egress of Alphaherpesviruses: Comparative Ultrastructural Study. J. Virol. 75: 3675-3684 [Abstract] [Full Text]
McMillan, T. N., Johnson, D. C. (2001). Cytoplasmic Domain of Herpes Simplex Virus gE Causes Accumulation in the trans-Golgi Network, a Site of Virus Envelopment and Sorting of Virions to Cell Junctions. J. Virol. 75: 1928-1940 [Abstract] [Full Text]
Kasai, K., Akagawa, K. (2001). Roles of the cytoplasmic and transmembrane domains of syntaxins in intracellular localization and trafficking. J. Cell Sci. 114: 3115-3124 [Abstract] [Full Text]
Varlamov, O, Kalinina, E, Che, F., Fricker, L. (2001). Protein phosphatase 2A binds to the cytoplasmic tail of carboxypeptidase D and regulates post-trans-Golgi network trafficking. J. Cell Sci. 114: 311-322 [Abstract]
Zhang, X. D., Franco, A. V., Nguyen, T., Gray, C. P., Hersey, P. (2000). Differential Localization and Regulation of Death and Decoy Receptors for TNF-Related Apoptosis-Inducing Ligand (TRAIL) in Human Melanoma Cells. J. Immunol. 164: 3961-3970 [Abstract] [Full Text]
Robertson, A. M., Allan, V. J. (2000). Brefeldin A-dependent Membrane Tubule Formation Reconstituted In Vitro Is Driven by a Cell Cycle-regulated Microtubule Motor. Mol. Biol. Cell 11: 941-955 [Abstract] [Full Text]
Dressman, M. A., Olivos–Glander, I. M., Nussbaum, R. L., Suchy, S. F. (2000). Ocrl1, a PtdIns(4,5)P2 5-Phosphatase, Is Localized to the Trans-Golgi Network of Fibroblasts and Epithelial Cells. J. Histochem. Cytochem. 48: 179-190 [Abstract] [Full Text]
Prekeris, R., Foletti, D. L., Scheller, R. H. (1999). Dynamics of Tubulovesicular Recycling Endosomes in Hippocampal Neurons. J. Neurosci. 19: 10324-10337 [Abstract] [Full Text]
Advani, R. J., Yang, B., Prekeris, R., Lee, K. C., Klumperman, J., Scheller, R. H. (1999). Vamp-7 Mediates Vesicular Transport from Endosomes to Lysosomes. JCB 146: 765-776 [Abstract] [Full Text]
Steegmaier, M., Klumperman, J., Foletti, D. L., Yoo, J.-S., Scheller, R. H. (1999). Vesicle-associated Membrane Protein 4�is Implicated in Trans-Golgi Network Vesicle Trafficking. Mol. Biol. Cell 10: 1957-1972 [Abstract] [Full Text]
Jones, S. J., Ledgerwood, E. C., Prins, J. B., Galbraith, J., Johnson, D. R., Pober, J. S., Bradley, J. R. (1999). TNF Recruits TRADD to the Plasma Membrane But Not the trans-Golgi Network, the Principal Subcellular Location of TNF-R1. J. Immunol. 162: 1042-1048 [Abstract] [Full Text]
Alconada, A., Bauer, U., Sodeik, B., Hoflack, B. (1999). Intracellular Traffic of Herpes Simplex Virus Glycoprotein gE: Characterization of the Sorting Signals Required for Its trans-Golgi Network Localization. J. Virol. 73: 377-387 [Abstract] [Full Text]
Eng, F. J., Varlamov, O., Fricker, L. D. (1999). Sequences within the Cytoplasmic Domain of Gp180/Carboxypeptidase D Mediate Localization to the Trans-Golgi Network. Mol. Biol. Cell 10: 35-46 [Abstract] [Full Text]
Prekeris, R., Klumperman, J., Chen, Y. A., Scheller, R. H. (1998). Syntaxin 13 Mediates Cycling of Plasma Membrane Proteins via Tubulovesicular Recycling Endosomes. JCB 143: 957-971 [Abstract] [Full Text]
Roquemore, E. P., Banting, G. (1998). Efficient Trafficking of TGN38 from the Endosome to the trans-Golgi Network Requires a Free Hydroxyl Group at Position 331�in the Cytosolic Domain. Mol. Biol. Cell 9: 2125-2144 [Abstract] [Full Text]
Bose, S., Chapin, S. J., Seetharam, S., Feix, J., Mostov, K. E., Seetharam, B. (1998). Brefeldin A (BFA) Inhibits Basolateral Membrane (BLM) Delivery and Dimerization of Transcobalamin II Receptor in Human Intestinal Epithelial Caco-2 Cells. BFA EFFECTS ON BLM CHOLESTEROL CONTENT. J. Biol. Chem. 273: 16163-16169 [Abstract] [Full Text]
Reaves, B. J., Banting, G., Luzio, J. P. (1998). Lumenal and Transmembrane Domains Play a Role in Sorting Type I Membrane Proteins on Endocytic Pathways. Mol. Biol. Cell 9: 1107-1122 [Abstract] [Full Text]
Tang, B. L., Tan, A. E.�H., Lim, L. K., Lee, S. S., Low, D. Y.�H., Hong, W. (1998). Syntaxin 12,�a Member of the Syntaxin Family Localized to the Endosome. J. Biol. Chem. 273: 6944-6950 [Abstract] [Full Text]
Lannert, H., Gorgas, K., Meissner, I., Wieland, F. T., Jeckel, D. (1998). Functional Organization of the Golgi Apparatus in Glycosphingolipid Biosynthesis. LACTOSYLCERAMIDE AND SUBSEQUENT GLYCOSPHINGOLIPIDS ARE FORMED IN THE LUMEN OF THE LATE GOLGI. J. Biol. Chem. 273: 2939-2946 [Abstract] [Full Text]
Martin, P. E.�M., George, C. H., Castro, C., Kendall, J. M., Capel, J., Campbell, A. K., Revilla, A., Barrio, L. C., Evans, W. H. (1998). Assembly of Chimeric Connexin-Aequorin Proteins into Functional Gap Junction Channels. REPORTING INTRACELLULAR AND PLASMA MEMBRANE CALCIUM ENVIRONMENTS. J. Biol. Chem. 273: 1719-1726 [Abstract] [Full Text]
Varlamov, O, Fricker, L. (1998). Intracellular trafficking of metallocarboxypeptidase D in AtT-20 cells: localization to the trans-Golgi network and recycling from the cell surface. J. Cell Sci. 111: 877-885 [Abstract]
Drecktrah, D, de Figueiredo, P, Mason, R., Brown, W. (1998). Retrograde trafficking of both Golgi complex and TGN markers to the ER induced by nordihydroguaiaretic acid and cyclofenil diphenol. J. Cell Sci. 111: 951-965 [Abstract]
Girotti, M, Banting, G (1996). TGN38-green fluorescent protein hybrid proteins expressed in stably transfected eukaryotic cells provide a tool for the real-time, in vivo study of membrane traffic pathways and suggest a possible role for ratTGN38. J. Cell Sci. 109: 2915-2926 [Abstract]
Reaves, B., Bright, N., Mullock, B., Luzio, J. (1996). The effect of wortmannin on the localisation of lysosomal type I integral membrane glycoproteins suggests a role for phosphoinositide 3-kinase activity in regulating membrane traffic late in the endocytic pathway. J. Cell Sci. 109: 749-762 [Abstract]
Lehel, C., Ol�h, Z.�n, Jakab, G�b., Sz�ll�si, Z.�n, Petrovics, G.�r., Harta, G.�n., Blumberg, P. M., Anderson, W. B. (1995). Protein Kinase C [IMAGE] Subcellular Localization Domains and Proteolytic Degradation Sites. J. Biol. Chem. 270: 19651-19658 [Abstract] [Full Text]
Walker, J, Watson, J, Holmes, C, Edelman, A, Banting, G (1995). Production and characterisation of monoclonal and polyclonal antibodies to different regions of the cystic fibrosis transmembrane conductance regulator (CFTR): detection of immunologically related proteins. J. Cell Sci. 108: 2433-2444 [Abstract]
Johnston, P., Stieber, A, Gonatas, N. (1994). A hypothesis on the traffic of MG160, a medial Golgi sialoglycoprotein, from the trans-Golgi network to the Golgi cisternae. J. Cell Sci. 107: 529-537 [Abstract]
Turner, M., Handel, S., Wilde, C., Burgoyne, R. (1993). Differential effect of brefeldin A on phosphorylation of the caseins in lactating mouse mammary epithelial cells. J. Cell Sci. 106: 1221-1226 [Abstract]
Roa, M, Cornet, V, Yang, C., Goud, B (1993). The small GTP-binding protein rab6p is redistributed in the cytosol by brefeldin A. J. Cell Sci. 106: 789-802 [Abstract]
Satiat-Jeunemaitre, B., Hawes, C. (1992). Redistribution of a Golgi glycoprotein in plant cells treated with Brefeldin A. J. Cell Sci. 103: 1153-1166 [Abstract]
Antony, C., Cibert, C., Geraud, G., Santa Maria, A., Maro, B., Mayau, V., Goud, B. (1992). The small GTP-binding protein rab6p is distributed from medial Golgi to the trans-Golgi network as determined by a confocal microscopic approach. J. Cell Sci. 103: 785-796 [Abstract]
Milland, J., Taylor, S. G., Dodson, H. C., McKenzie, I. F. C., Sandrin, M. S. (2001). The Cytoplasmic Tail of alpha 1,2-Fucosyltransferase Contains a Sequence for Golgi Localization. J. Biol. Chem. 276: 12012-12018 [Abstract] [Full Text]
Misumi, Y., Sohda, M., Tashiro, A., Sato, H., Ikehara, Y. (2001). An Essential Cytoplasmic Domain for the Golgi Localization of Coiled-coil Proteins with a COOH-terminal Membrane Anchor. J. Biol. Chem. 276: 6867-6873 [Abstract] [Full Text]
Orzech, E., Livshits, L., Leyt, J., Okhrimenko, H., Reich, V., Cohen, S., Weiss, A., Melamed-Book, N., Lebendiker, M., Altschuler, Y., Aroeti, B. (2001). Interactions between Adaptor Protein-1 of the Clathrin Coat and Microtubules via Type 1a Microtubule-associated Proteins. J. Biol. Chem. 276: 31340-31348 [Abstract] [Full Text]