Cyclins A and B associate with chromatin and the polar regions of spindles, respectively, and do not undergo complete degradation at anaphase in syncytial Drosophila embryos

doi:10.1083/jcb.116.4.967

This Article

	Full Text (PDF, 3193K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Maldonado-Codina, G.
	Articles by Glover, D. M.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Maldonado-Codina, G.
	Articles by Glover, D. M.


Social Bookmarking

	What's this?

ARTICLES

Cyclins A and B associate with chromatin and the polar regions of spindles, respectively, and do not undergo complete degradation at anaphase in syncytial Drosophila embryos

G Maldonado-Codina and DM Glover
Department of Biochemistry, University of Dundee, Scotland.

Maternally contributed cyclin A and B proteins are initially distributed uniformly throughout the syncytial Drosophila embryo. As dividing nuclei migrate to the cortex of the embryo, the A and B cyclins become concentrated in surface layers extending to depths of approximately 30-40 microns and 5-10 microns, respectively. The initiation of nuclear envelope breakdown, spindle formation, and the initial congression of the centromeric regions of the chromosomes onto the metaphase plate all take place within the surface layer occupied by cyclin B on the apical side of the blastoderm nuclei. Cyclin B is seen mainly, but not exclusively, in the vicinity of microtubules throughout the mitotic cycle. It is most conspicuous around the centrosomes. Cyclin A is present at its highest concentrations throughout the cytoplasm during the interphase periods of the blastoderm cycles, although weak punctate staining can also be detected in the nucleus. It associates with the condensing chromosomes during prophase, segregates into daughter nuclei in association with chromosomes during anaphase, to redistribute into the cytoplasm after telophase. In contrast to the cycles following cellularization, neither cyclin is completely degraded upon the metaphase-anaphase transition.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Zhang, X.-H., Axton, J. M., Drinjakovic, J., Lorenz, L., White-Cooper, H., Renault, A. D. (2004). Spatial and temporal control of mitotic cyclins by the Gnu regulator of embryonic mitosis in Drosophila. J. Cell Sci. 117: 3571-3578 [Abstract] [Full Text]
Ji, J.-Y., Haghnia, M., Trusty, C., Goldstein, L. S. B., Schubiger, G. (2002). A Genetic Screen for Suppressors and Enhancers of the Drosophila Cdk1-Cyclin B Identifies Maternal Factors That Regulate Microtubule and Microfilament Stability. Genetics 162: 1179-1195 [Abstract] [Full Text]
Douglas, R. M., Xu, T., Haddad, G. G. (2001). Cell cycle progression and cell division are sensitive to hypoxia in Drosophila melanogaster embryos. Am. J. Physiol. Regul. Integr. Comp. Physiol. 280: R1555-R1563 [Abstract] [Full Text]
Takada, S., Shibata, T., Hiraoka, Y., Masuda, H. (2000). Identification of Ribonucleotide Reductase Protein R1 as an Activator of Microtubule Nucleation in Xenopus Egg Mitotic Extracts. Mol. Biol. Cell 11: 4173-4187 [Abstract] [Full Text]
Stiffler, L., Ji, J., Trautmann, S, Trusty, C, Schubiger, G (1999). Cyclin A and B functions in the early Drosophila embryo. Development 126: 5505-5513 [Abstract]
Su, T. T., Sprenger, F., DiGregorio, P. J., Campbell, S. D., O'Farrell, P. H. (1998). Exit from mitosis in Drosophila syncytial embryos requires proteolysis and cyclin degradation, and is associated with localized�dephosphorylation. Genes Dev. 12: 1495-1503 [Abstract] [Full Text]
Brandeis, M., Rosewell, I., Carrington, M., Crompton, T., Jacobs, M. A., Kirk, J., Gannon, J., Hunt, T. (1998). Cyclin B2-null mice develop normally and are fertile whereas cyclin B1-null mice die in utero. Proc. Natl. Acad. Sci. USA 95: 4344-4349 [Abstract] [Full Text]
Bridge, A. J., Morphew, M., Bartlett, R., Hagan, I. M. (1998). The fission yeast SPB component Cut12 links bipolar spindle formation to mitotic�control. Genes Dev. 12: 927-942 [Abstract] [Full Text]
Mathe, E, Boros, I, Josvay, K, Li, K, Puro, J, Kaufman, T., Szabad, J (1998). The Tomaj mutant alleles of alpha Tubulin67C reveal a requirement for the encoded maternal specific tubulin isoform in the sperm aster, the cleavage spindle apparatus and neurogenesis during embryonic development in Drosophila. J. Cell Sci. 111: 887-896 [Abstract]
Roghi, C, Giet, R, Uzbekov, R, Morin, N, Chartrain, I, Le Guellec, R, Couturier, A, Doree, M, Philippe, M, Prigent, C (1998). The Xenopus protein kinase pEg2 associates with the centrosome in a cell cycle-dependent manner, binds to the spindle microtubules and is involved in bipolar mitotic spindle assembly. J. Cell Sci. 111: 557-572 [Abstract]
Kidd, D, Raff, J. (1997). LK6, a short lived protein kinase in Drosophila that can associate with microtubules and centrosomes. J. Cell Sci. 110: 209-219 [Abstract]
Brisch, E, Daggett, M., Suprenant, K. (1996). Cell cycle-dependent phosphorylation of the 77 kDa echinoderm microtubule-associated protein (EMAP) in vivo and association with the p34cdc2 kinase. J. Cell Sci. 109: 2885-2893 [Abstract]
Masuda, H, Shibata, T (1996). Role of gamma-tubulin in mitosis-specific microtubule nucleation from the Schizosaccharomyces pombe spindle pole body. J. Cell Sci. 109: 165-177 [Abstract]
Girard, F, Fernandez, A, Lamb, N (1995). Delayed cyclin A and B1 degradation in non-transformed mammalian cells. J. Cell Sci. 108: 2599-2608 [Abstract]
Edgar, B A, Sprenger, F, Duronio, R J, Leopold, P, O'Farrell, P H (1994). Distinct molecular mechanism regulate cell cycle timing at successive stages of Drosophila embryogenesis.. Genes Dev. 8: 440-452 [Abstract]
Rimmington, G, Dalby, B, Glover, D. (1994). Expression of N-terminally truncated cyclin B in the Drosophila larval brain leads to mitotic delay at late anaphase. J. Cell Sci. 107: 2729-2738 [Abstract]
White-Cooper, H, Alphey, L, Glover, D. (1993). The cdc25 homologue twine is required for only some aspects of the entry into meiosis in Drosophila. J. Cell Sci. 106: 1035-1044 [Abstract]
Maridor, G, Gallant, P, Golsteyn, R, Nigg, E. (1993). Nuclear localization of vertebrate cyclin A correlates with its ability to form complexes with cdk catalytic subunits. J. Cell Sci. 106: 535-544 [Abstract]
Philp, A., Axton, J., Saunders, R., Glover, D. (1993). Mutations in the Drosophila melanogaster gene three rows permit aspects of mitosis to continue in the absence of chromatid segregation. J. Cell Sci. 106: 87-98 [Abstract]
Ookata, K, Hisanaga, S, Okumura, E, Kishimoto, T (1993). Association of p34cdc2/cyclin B complex with microtubules in starfish oocytes. J. Cell Sci. 105: 873-881 [Abstract]
Maldonado-Codina, G, Llamazares, S, Glover, D. (1993). Heat shock results in cell cycle delay and synchronisation of mitotic domains in cellularised Drosophila melanogaster embryos. J. Cell Sci. 105: 711-720 [Abstract]
Rothbarth, K, Petzelt, C, Lu, X, Todorov, I., Joswig, G, Pepperkok, R, Ansorge, W, Werner, D (1993). cDNA-derived molecular characteristics and antibodies to a new centrosome-associated and G2/M phase-prevalent protein. J. Cell Sci. 104: 19-30 [Abstract]
Ohta, K, Shiina, N, Okumura, E, Hisanaga, S, Kishimoto, T, Endo, S, Gotoh, Y, Nishida, E, Sakai, H (1993). Microtubule nucleating activity of centrosomes in cell-free extracts from Xenopus eggs: involvement of phosphorylation and accumulation of pericentriolar material. J. Cell Sci. 104: 125-137 [Abstract]
Dawson, I. A., Roth, S., Akam, M., Artavanis-Tsakonas, S. (1993). Mutations of the fizzy locus cause metaphase arrest in Drosophila melanogaster embryos. Development 117: 359-376 [Abstract]