Molecular organization of the uvomorulin-catenin complex

doi:10.1083/jcb.116.4.989

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Molecular organization of the uvomorulin-catenin complex

M Ozawa and R Kemler
Max-Planck-Institut fur Immunbiologie, FG Moleculare Embryologie, Freiburg, Germany.

The Ca(2+)-dependent cell adhesion molecule uvomorulin is a member of the cadherin gene family. Its cytoplasmic region complexes with structurally defined proteins termed alpha-, beta-, and gamma-catenins. Here we show that A-CAM (N-cadherin), another member of this gene family, also associates with catenins suggesting that this complex formation may be a general property of the cadherins. For uvomorulin it has been found that this association with catenins is of crucial importance for the adhesive function, but little is known about the molecular organization of the uvomorulin-catenin complex. Using a combination of biochemical analyses we show that a single complex is composed of one molecule of uvomorulin, one or two molecules of beta- catenin, and one molecule of alpha-catenin. Furthermore, beta-catenin seems to interact more directly with uvomorulin. In pulse-chase experiments beta-catenin is already associated with the 135-kD uvomorulin precursor molecule but the assembly of the newly synthesized alpha-catenin into the complex is only detected around the time of endoproteolytic processing.
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Shan, W., Yagita, Y., Wang, Z., Koch, A., Svenningsen, A. F., Gruzglin, E., Pedraza, L., Colman, D. R. (2004). The Minimal Essential Unit for Cadherin-mediated Intercellular Adhesion Comprises Extracellular Domains 1 and 2. J. Biol. Chem. 279: 55914-55923 [Abstract] [Full Text]
Faleiro-Rodrigues, C., Macedo-Pinto, I., Pereira, D., Lopes, C. S. (2004). Prognostic value of E-cadherin immunoexpression in patients with primary ovarian carcinomas. Ann Oncol 15: 1535-1542 [Abstract] [Full Text]
Chan, M. W. C., El Sayegh, T. Y., Arora, P. D., Laschinger, C. A., Overall, C. M., Morrison, C., McCulloch, C. A. G. (2004). Regulation of Intercellular Adhesion Strength in Fibroblasts. J. Biol. Chem. 279: 41047-41057 [Abstract] [Full Text]
de Vries, W. N., Evsikov, A. V., Haac, B. E., Fancher, K. S., Holbrook, A. E., Kemler, R., Solter, D., Knowles, B. B. (2004). Maternal {beta}-catenin and E-cadherin in mouse development. Development 131: 4435-4445 [Abstract] [Full Text]
Dey, S. K., Lim, H., Das, S. K., Reese, J., Paria, B. C., Daikoku, T., Wang, H. (2004). Molecular Cues to Implantation. Endocr. Rev. 25: 341-373 [Abstract] [Full Text]
Calvisi, D. F., Ladu, S., Factor, V. M., Thorgeirsson, S. S. (2004). Activation of {beta}-catenin provides proliferative and invasive advantages in c-myc/TGF-{alpha} hepatocarcinogenesis promoted by phenobarbital. Carcinogenesis 25: 901-908 [Abstract] [Full Text]
Vincent, P. A., Xiao, K., Buckley, K. M., Kowalczyk, A. P. (2004). VE-cadherin: adhesion at arm's length. Am. J. Physiol. Cell Physiol. 286: C987-C997 [Abstract] [Full Text]
DeBellefeuille, S., Hermo, L., Gregory, M., Dufresne, J., Cyr, D. G. (2003). Catenins in the Rat Epididymis: Their Expression and Regulation in Adulthood and during Postnatal Development. Endocrinology 144: 5040-5049 [Abstract] [Full Text]
Miranda, K. C., Joseph, S. R., Yap, A. S., Teasdale, R. D., Stow, J. L. (2003). Contextual Binding of p120ctn to E-cadherin at the Basolateral Plasma Membrane in Polarized Epithelia. J. Biol. Chem. 278: 43480-43488 [Abstract] [Full Text]
Wahl, J. K. III, Kim, Y. J., Cullen, J. M., Johnson, K. R., Wheelock, M. J. (2003). N-cadherin-Catenin Complexes Form Prior to Cleavage of the Proregion and Transport to the Plasma Membrane. J. Biol. Chem. 278: 17269-17276 [Abstract] [Full Text]
Martin, B., Schneider, R., Janetzky, S., Waibler, Z., Pandur, P., Kuhl, M., Behrens, J., von der Mark, K., Starzinski-Powitz, A., Wixler, V. (2002). The LIM-only protein FHL2 interacts with {beta}-catenin and promotes differentiation of mouse myoblasts. JCB 159: 113-122 [Abstract] [Full Text]
Tran, N. L., Adams, D. G., Vaillancourt, R. R., Heimark, R. L. (2002). Signal Transduction from N-cadherin Increases Bcl-2. REGULATION OF THE PHOSPHATIDYLINOSITOL 3-KINASE/Akt PATHWAY BY HOMOPHILIC ADHESION AND ACTIN CYTOSKELETAL ORGANIZATION. J. Biol. Chem. 277: 32905-32914 [Abstract] [Full Text]
Le, T. L., Joseph, S. R., Yap, A. S., Stow, J. L. (2002). Protein kinase C regulates endocytosis and recycling of E-cadherin. Am. J. Physiol. Cell Physiol. 283: C489-C499 [Abstract] [Full Text]
Palmen, T., Lehtonen, S., Ora, A., Kerjaschki, D., Antignac, C., Lehtonen, E., Holthofer, H. (2002). Interaction of Endogenous Nephrin and CD2-Associated Protein in Mouse Epithelial M-1 Cell Line. J. Am. Soc. Nephrol. 13: 1766-1772 [Abstract] [Full Text]
Pokutta, S., Drees, F., Takai, Y., Nelson, W. J., Weis, W. I. (2002). Biochemical and Structural Definition of the l-Afadin- and Actin-binding Sites of alpha -Catenin. J. Biol. Chem. 277: 18868-18874 [Abstract] [Full Text]
Prozialeck, W. C., Fay, M. J., Lamar, P. C., Pearson, C. A., Sigar, I., Ramsey, K. H. (2002). Chlamydia trachomatis Disrupts N-Cadherin-Dependent Cell-Cell Junctions and Sequesters {beta}-Catenin in Human Cervical Epithelial Cells. Infect. Immun. 70: 2605-2613 [Abstract] [Full Text]
Matsubara, S., Ozawa, M. (2001). Expression of {alpha}-catenin in {alpha}-catenin-deficient cells increases resistance to sphingosine-induced apoptosis. JCB 154: 573-584 [Abstract] [Full Text]
Decaens, C., Cassio, D. (2001). Spatiotemporal expression of catenins, ZO-1, and occludin during early polarization of hepatic WIF-B9 cells. Am. J. Physiol. Cell Physiol. 280: C527-C539 [Abstract] [Full Text]
Ozawa, M, Ohkubo, T (2001). Tyrosine phosphorylation of p120(ctn) in v-Src transfected L cells depends on its association with E-cadherin and reduces adhesion activity. J. Cell Sci. 114: 503-512 [Abstract]
Chitaev, N., Averbakh, A., Troyanovsky, R., Troyanovsky, S. (2000). Molecular organization of the desmoglein-plakoglobin complex. J. Cell Sci. 111: 1941-1949 [Abstract]
Yu, J, Ebert, M P A, Miehlke, S, Rost, H, Lendeckel, U, Leodolter, A, Stolte, M, Bayerdorffer, E, Malfertheiner, P (2000). alpha -Catenin expression is decreased in human gastric cancers and in the gastric mucosa of first degree relatives. Gut 46: 639-644 [Abstract] [Full Text]
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Hanakawa, Y, Amagai, M, Shirakata, Y, Sayama, K, Hashimoto, K (2000). Different effects of dominant negative mutants of desmocollin and desmoglein on the cell-cell adhesion of keratinocytes. J. Cell Sci. 113: 1803-1811 [Abstract]
Ohkubo, T., Ozawa, M. (1999). p120ctn Binds to the Membrane-proximal Region of the E-cadherin Cytoplasmic Domain and Is Involved in Modulation of Adhesion Activity. J. Biol. Chem. 274: 21409-21415 [Abstract] [Full Text]
Pece, S., Chiariello, M., Murga, C., Gutkind, J. S. (1999). Activation of the Protein Kinase Akt/PKB by the Formation of E-cadherin-mediated Cell-Cell Junctions. EVIDENCE FOR THE ASSOCIATION OF PHOSPHATIDYLINOSITOL 3-KINASE WITH THE E-CADHERIN ADHESION COMPLEX. J. Biol. Chem. 274: 19347-19351 [Abstract] [Full Text]
Jesuthasan, S (1999). Furrow-associated microtubule arrays are required for the cohesion of zebrafish blastomeres following cytokinesis. J. Cell Sci. 111: 3695-3703 [Abstract]
Kang, D. E., Soriano, S., Frosch, M. P., Collins, T., Naruse, S., Sisodia, S. S., Leibowitz, G., Levine, F., Koo, E. H. (1999). Presenilin 1�Facilitates the Constitutive Turnover of beta -Catenin: Differential Activity of Alzheimer's Disease-Linked PS1 Mutants in the beta -Catenin-Signaling Pathway. J. Neurosci. 19: 4229-4237 [Abstract] [Full Text]
Pötter, E., Bergwitz, C., Brabant, G. (1999). The Cadherin-Catenin System: Implications for Growth and Differentiation of Endocrine Tissues. Endocr. Rev. 20: 207-239 [Abstract] [Full Text]
Itoh, M., Morita, K., Tsukita, S. (1999). Characterization of ZO-2 as a MAGUK Family Member Associated with Tight as well as Adherens Junctions with a Binding Affinity to Occludin and alpha �Catenin. J. Biol. Chem. 274: 5981-5986 [Abstract] [Full Text]
Chen, Y.-T., Stewart, D. B., Nelson, W. J. (1999). Coupling Assembly of the E-Cadherin/{beta}-Catenin Complex to Efficient Endoplasmic Reticulum Exit and Basal-lateral Membrane Targeting of E-Cadherin in Polarized MDCK Cells. JCB 144: 687-699 [Abstract] [Full Text]
Huber, O, Kemler, R, Langosch, D (1999). Mutations affecting transmembrane segment interactions impair adhesiveness of E-cadherin. J. Cell Sci. 112: 4415-4423 [Abstract]
Zhu, A., Watt, F. (1999). beta-catenin signalling modulates proliferative potential of human epidermal keratinocytes independently of intercellular adhesion. Development 126: 2285-2298 [Abstract]
Ozawa, M., Kemler, R. (1998). The Membrane-proximal Region of the E-Cadherin Cytoplasmic Domain Prevents Dimerization and Negatively Regulates Adhesion Activity. JCB 142: 1605-1613 [Abstract] [Full Text]
Adams, C. L., Chen, Y.-T., Smith, S. J, James Nelson, W. (1998). Mechanisms of Epithelial Cell-Cell Adhesion and Cell Compaction Revealed by High-resolution Tracking of E-Cadherin- Green Fluorescent Protein. JCB 142: 1105-1119 [Abstract] [Full Text]
Chitaev, N. A., Troyanovsky, S. M. (1998). Adhesive But Not Lateral E-cadherin Complexes Require Calcium and Catenins for Their Formation. JCB 142: 837-846 [Abstract] [Full Text]
Batt, D. B., Roberts, T. M. (1998). Cell Density Modulates Protein-tyrosine Phosphorylation. J. Biol. Chem. 273: 3408-3414 [Abstract] [Full Text]
Kowalczyk, A., Navarro, P, Dejana, E, Bornslaeger, E., Green, K., Kopp, D., Borgwardt, J. (1998). VE-cadherin and desmoplakin are assembled into dermal microvascular endothelial intercellular junctions: a pivotal role for plakoglobin in the recruitment of desmoplakin to intercellular junctions. J. Cell Sci. 111: 3045-3057 [Abstract]
Norvell, S., Green, K. (1998). Contributions of extracellular and intracellular domains of full length and chimeric cadherin molecules to junction assembly in epithelial cells. J. Cell Sci. 111: 1305-1318 [Abstract]
Li, Z, Gallin, W., Lauzon, G, Pasdar, M (1998). L-CAM expression induces fibroblast-epidermoid transition in squamous carcinoma cells and down-regulates the endogenous N-cadherin. J. Cell Sci. 111: 1005-1019 [Abstract]
Marcozzi, C, Burdett, I., Buxton, R., Magee, A. (1998). Coexpression of both types of desmosomal cadherin and plakoglobin confers strong intercellular adhesion. J. Cell Sci. 111: 495-509 [Abstract]
Litvinov, S. V., Balzar, M., Winter, M. J., Bakker, H. A.M., Bruijn, I. H. B.-d., Prins, F., Fleuren, G. J., Warnaar, S. O. (1997). Epithelial Cell Adhesion Molecule (Ep-CAM) Modulates Cell-Cell Interactions Mediated by Classic Cadherins. JCB 139: 1337-1348 [Abstract] [Full Text]
Koslov, E. R., Maupin, P., Pradhan, D., Morrow, J. S., Rimm, D. L. (1997). alpha -Catenin Can Form Asymmetric Homodimeric Complexes and/or Heterodimeric Complexes with beta -Catenin. J. Biol. Chem. 272: 27301-27306 [Abstract] [Full Text]
Redfield, A., Nieman, M. T., Knudsen, K. A. (1997). Cadherins Promote Skeletal Muscle Differentiation in Three-dimensional Cultures. JCB 138: 1323-1331 [Abstract] [Full Text]
Itoh, M., Nagafuchi, A., Moroi, S., Tsukita, S. (1997). Involvement of ZO-1 in Cadherin-based Cell Adhesion through Its Direct Binding to {alpha} Catenin and Actin Filaments. JCB 138: 181-192 [Abstract] [Full Text]
Finnemann, S., Mitrik, I., Hess, M., Otto, G., Wedlich, D. (1997). Uncoupling of XB/U-Cadherin-Catenin Complex Formation from Its Function in Cell-Cell Adhesion. J. Biol. Chem. 272: 11856-11862 [Abstract] [Full Text]
Barth, A. I.M., Pollack, A. L., Altschuler, Y., Mostov, K. E., Nelson, W. J. (1997). NH2-terminal Deletion of {beta}-Catenin Results in Stable Colocalization of Mutant {beta}-Catenin with Adenomatous Polyposis Coli Protein and Altered MDCK Cell Adhesion. JCB 136: 693-706 [Abstract] [Full Text]
Kofron, M, Spagnuolo, A, Klymkowsky, M, Wylie, C, Heasman, J (1997). The roles of maternal alpha-catenin and plakoglobin in the early Xenopus embryo. Development 124: 1553-1560 [Abstract]
Wang, Y, Rose, B (1997). An inhibition of gap-junctional communication by cadherins. J. Cell Sci. 110: 301-309 [Abstract]
Chen, H, Paradies, N., Fedor-Chaiken, M, Brackenbury, R (1997). E-cadherin mediates adhesion and suppresses cell motility via distinct mechanisms. J. Cell Sci. 110: 345-356 [Abstract]
Miller, J R, Moon, R T (1996). Signal transduction through beta-catenin and specification of cell fate during embryogenesis.. Genes Dev. 10: 2527-2539
Fuchs, M., Muller, T., Lerch, M. M., Ullrich, A. (1996). Association of Human Protein-tyrosine Phosphatase kappa with Members of the Armadillo Family. J. Biol. Chem. 271: 16712-16719 [Abstract] [Full Text]
Kitajima, I., Kawahara, K., Hanyu, N., Shin, H., Tokioka, T., Soejima, Y., Tsutsui, J., Ozawa, M., Shimayama, T., Maruyama, I. (1996). Enhanced E-cadherin expression and increased calcium-dependent cell-cell adhesion in human T-cell leukemia virus type I Tax-expressing PC12 cells. J. Cell Sci. 109: 609-617 [Abstract]
Wahl, J., Sacco, P., McGranahan-Sadler, T., Sauppe, L., Wheelock, M., Johnson, K. (1996). Plakoglobin domains that define its association with the desmosomal cadherins and the classical cadherins: identification of unique and shared domains. J. Cell Sci. 109: 1143-1154 [Abstract]
Hertig, C., Butz, S, Koch, S, Eppenberger-Eberhardt, M, Kemler, R, Eppenberger, H. (1996). N-cadherin in adult rat cardiomyocytes in culture. II. Spatio-temporal appearance of proteins involved in cell-cell contact and communication. Formation of two distinct N-cadherin/catenin complexes. J. Cell Sci. 109: 11-20 [Abstract]
Sacco, P. A., McGranahan, T. M., Wheelock, M. J., Johnson, K. R. (1995). Identification of Plakoglobin Domains Required for Association with N-cadherin and alpha-Catenin. J. Biol. Chem. 270: 20201-20206 [Abstract] [Full Text]
Monier-Gavelle, F, Duband, J. (1995). Control of N-cadherin-mediated intercellular adhesion in migrating neural crest cells in vitro. J. Cell Sci. 108: 3839-3853 [Abstract]
Haegel, H, Larue, L, Ohsugi, M, Fedorov, L, Herrenknecht, K, Kemler, R (1995). Lack of beta-catenin affects mouse development at gastrulation. Development 121: 3529-3537 [Abstract]
Zeschnigk, M, Kozian, D, Kuch, C, Schmoll, M, Starzinski-Powitz, A (1995). Involvement of M-cadherin in terminal differentiation of skeletal muscle cells. J. Cell Sci. 108: 2973-2981 [Abstract]
Aberle, H, Butz, S, Stappert, J, Weissig, H, Kemler, R, Hoschuetzky, H (1994). Assembly of the cadherin-catenin complex in vitro with recombinant proteins. J. Cell Sci. 107: 3655-3663 [Abstract]
Fagotto, F, Gumbiner, B. (1994). Beta-catenin localization during Xenopus embryogenesis: accumulation at tissue and somite boundaries. Development 120: 3667-3679 [Abstract]
Tanihara, H, Kido, M, Obata, S, Heimark, R., Davidson, M, St John, T, Suzuki, S (1994). Characterization of cadherin-4 and cadherin-5 reveals new aspects of cadherins. J. Cell Sci. 107: 1697-1704 [Abstract]
Wolburg, H, Neuhaus, J, Kniesel, U, Krauss, B, Schmid, E., Ocalan, M, Farrell, C, Risau, W (1994). Modulation of tight junction structure in blood-brain barrier endothelial cells. Effects of tissue culture, second messengers and cocultured astrocytes. J. Cell Sci. 107: 1347-1357 [Abstract]
Navarro, P, Lozano, E, Cano, A (1993). Expression of E- or P-cadherin is not sufficient to modify the morphology and the tumorigenic behavior of murine spindle carcinoma cells. Possible involvement of plakoglobin. J. Cell Sci. 105: 923-934 [Abstract]
Peifer, M (1993). The product of the Drosophila segment polarity gene armadillo is part of a multi-protein complex resembling the vertebrate adherens junction. J. Cell Sci. 105: 993-1000 [Abstract]
Schneider, S, Herrenknecht, K, Butz, S, Kemler, R, Hausen, P (1993). Catenins in Xenopus embryogenesis and their relation to the cadherin-mediated cell-cell adhesion system. Development 118: 629-640 [Abstract]
Takeichi, M., Hirano, S., Matsuyoshi, N., Fujimori, T. (1992). Cytoplasmic Control of Cadherin-mediated Cell-Cell Adhesion. Cold Spring Harb Symp Quant Biol 57: 327-334 [Abstract]
Pece, S., Gutkind, J. S. (2000). Signaling from E-cadherins to the MAPK Pathway by the Recruitment and Activation of Epidermal Growth Factor Receptors upon Cell-Cell Contact Formation. J. Biol. Chem. 275: 41227-41233 [Abstract] [Full Text]
Huber, A. H., Stewart, D. B., Laurents, D. V., Nelson, W. J., Weis, W. I. (2001). The Cadherin Cytoplasmic Domain Is Unstructured in the Absence of beta -Catenin. A POSSIBLE MECHANISM FOR REGULATING CADHERIN TURNOVER. J. Biol. Chem. 276: 12301-12309 [Abstract] [Full Text]
Niessen, C. M., Gumbiner, B. M. (2002). Cadherin-mediated cell sorting not determined by binding or adhesion specificity. JCB 156: 389-400 [Abstract] [Full Text]