Cortical localization of a calcium release channel in sea urchin eggs

doi:10.1083/jcb.116.5.1111

This Article

	Full Text (PDF, 1529K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by McPherson, S. M.
	Articles by Longo, F. J.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by McPherson, S. M.
	Articles by Longo, F. J.


Social Bookmarking

	What's this?

ARTICLES

Cortical localization of a calcium release channel in sea urchin eggs

SM McPherson, PS McPherson, L Mathews, KP Campbell and FJ Longo
Department of Anatomy, University of Iowa, College of Medicine, Iowa City 52242.

We have used an antibody against the ryanodine receptor/calcium release channel of skeletal muscle sarcoplasmic reticulum to localize a calcium release channel in sea urchin eggs. The calcium release channel is present in less than 20% of immature oocytes, where it does not demonstrate a specific cytoplasmic localization, while it is confined to the cortex of all mature eggs examined. This is in contrast to the cortical and subcortical localization of calsequestrin in mature and immature eggs. Immunolocalization of the calcium release channel reveals a cortical reticulum or honeycomb staining network that surrounds cortical granules and is associated with the plasma membrane. The network consists of some immunoreactive electron-dense material coating small vesicles and elongate cisternae of the endoplasmic reticulum. The fluorescent reticular staining pattern is lost when egg cortices are treated with agents known to affect sarcoplasmic reticulum calcium release and induce cortical granule exocytosis (ryanodine, calcium, A-23187, and caffeine). An approximately 380-kD protein of sea urchin egg cortices is identified by immunoblot analysis with the ryanodine receptor antibody. These results demonstrate: (a) the presence of a ryanodine-sensitive calcium release channel that is located within the sea urchin egg cortex; (b) an altered calcium release channel staining pattern as a result of treatments that initiate the cortical granule reaction; and (c) a spatial and functional dichotomy of the ER which may be important in serving different roles in the mobilization of calcium at fertilization.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Morgan, A. J., Galione, A. (2007). Fertilization and Nicotinic Acid Adenine Dinucleotide Phosphate Induce pH Changes in Acidic Ca2+ Stores in Sea Urchin Eggs. J. Biol. Chem. 282: 37730-37737 [Abstract] [Full Text]
Leckie, C., Empson, R., Becchetti, A., Thomas, J., Galione, A., Whitaker, M. (2003). The NO Pathway Acts Late during the Fertilization Response in Sea Urchin Eggs. J. Biol. Chem. 278: 12247-12254 [Abstract] [Full Text]
Balakier, H., Dziak, E., Sojecki, A., Librach, C., Michalak, M., Opas, M. (2002). Calcium-binding proteins and calcium-release channels in human maturing oocytes, pronuclear zygotes and early preimplantation embryos. Hum Reprod 17: 2938-2947 [Abstract] [Full Text]
Dumollard, R., Carroll, J., Dupont, G., Sardet, C. (2002). Calcium wave pacemakers in eggs. J. Cell Sci. 115: 3557-3564 [Abstract] [Full Text]
Kuroda, R., Kontani, K., Kanda, Y., Katada, T., Nakano, T., Satoh, Y.-i., Suzuki, N., Kuroda, H. (2001). Increase of cGMP, cADP-ribose and inositol 1,4,5-trisphosphate preceding Ca2+ transients in fertilization of sea urchin eggs. Development 128: 4405-4414 [Abstract] [Full Text]
Yazaki, I (2001). Ca(2+) in specification of vegetal cell fate in early sea urchin embryos. J. Exp. Biol. 204: 823-834 [Abstract]
Ito, M., Hirata, Y., Nakamura, H., Ohizumi, Y. (1999). Xestoquinone, Isolated from Sea Sponge, Causes Ca2+ Release through Sulfhydryl Modification from Skeletal Muscle Sarcoplasmic Reticulum. J. Pharmacol. Exp. Ther. 291: 976-981 [Abstract] [Full Text]
Fissore, R. A., Longo, F. J., Anderson, E., Parys, J. B., Ducibella, T. (1999). Differential Distribution of Inositol Trisphosphate Receptor Isoforms in Mouse Oocytes. Biol. Reprod. 60: 49-57 [Abstract] [Full Text]
Lokuta, A. J, Darszon, A., Beltran, C., Valdivia, H. H (1998). Detection and functional characterization of ryanodine receptors from sea urchin eggs. J. Physiol. 510: 155-164 [Abstract] [Full Text]
Zucchi, R., Ronca-Testoni, S. (1997). The Sarcoplasmic Reticulum Ca2+ Channel/Ryanodine Receptor: Modulation by Endogenous Effectors, Drugs and Disease States. Pharmacol. Rev. 49: 1-52 [Abstract] [Full Text]
Berg, L., Wessel, G. (1997). Cortical granules of the sea urchin translocate early in oocyte maturation. Development 124: 1845-1850 [Abstract]
Willmott, N., Sethi, J. K., Walseth, T. F., Lee, H. C., White, A. M., Galione, A. (1996). Nitric Oxide-induced Mobilization of Intracellular Calcium via the Cyclic ADP-ribose Signaling Pathway. J. Biol. Chem. 271: 3699-3705 [Abstract] [Full Text]
Jones, K. T., Carroll, J., Whittingham, D. G. (1995). Ionomycin, Thapsigargin, Ryanodine, and Sperm Induced Ca[IMAGE] Release Increase during Meiotic Maturation of Mouse Oocytes. J. Biol. Chem. 270: 6671-6677 [Abstract] [Full Text]
Ayabe, T, Kopf, G., Schultz, R. (1995). Regulation of mouse egg activation: presence of ryanodine receptors and effects of microinjected ryanodine and cyclic ADP ribose on uninseminated and inseminated eggs. Development 121: 2233-2244 [Abstract]
Stelly, N, Halpern, S, Nicolas, G, Fragu, P, Adoutte, A (1995). Direct visualization of a vast cortical calcium compartment in Paramecium by secondary ion mass spectrometry (SIMS) microscopy: possible involvement in exocytosis. J. Cell Sci. 108: 1895-1909 [Abstract]
Carroll, J, Swann, K, Whittingham, D, Whitaker, M (1994). Spatiotemporal dynamics of intracellular [Ca2+]i oscillations during the growth and meiotic maturation of mouse oocytes. Development 120: 3507-3517 [Abstract]
Galione, A, McDougall, A, Busa, W., Willmott, N, Gillot, I, Whitaker, M (1993). Redundant mechanisms of calcium-induced calcium release underlying calcium waves during fertilization of sea urchin eggs. Science 261: 348-352 [Abstract]
Churchill, G. C., Galione, A. (2000). Spatial Control of Ca2+ Signaling by Nicotinic Acid Adenine Dinucleotide Phosphate Diffusion and Gradients. J. Biol. Chem. 275: 38687-38692 [Abstract] [Full Text]
Shiwa, M., Murayama, T., Ogawa, Y. (2002). Molecular cloning and characterization of ryanodine receptor from unfertilized sea urchin eggs. Am. J. Physiol. Regul. Integr. Comp. Physiol. 282: R727-R737 [Abstract] [Full Text]