Tenascin promotes cerebellar granule cell migration and neurite outgrowth by different domains in the fibronectin type III repeats

doi:10.1083/jcb.116.6.1475

This Article

	Full Text (PDF, 2027K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Husmann, K.
	Articles by Schachner, M.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Husmann, K.
	Articles by Schachner, M.


Social Bookmarking

	What's this?

ARTICLES

Tenascin promotes cerebellar granule cell migration and neurite outgrowth by different domains in the fibronectin type III repeats

K Husmann, A Faissner and M Schachner
Department of Neurobiology, University of Heidelberg, Germany.

The extracellular matrix molecule tenascin has been implicated in neuron-glia recognition in the developing central and peripheral nervous system and in regeneration. In this study, its role in Bergmann glial process-mediated neuronal migration was assayed in vitro using tissue explants of the early postnatal mouse cerebellar cortex. Of the five mAbs reacting with nonoverlapping epitopes on tenascin, mAbs J1/tn1, J1/tn4, and J1/tn5, but not mAbs J1/tn2 and J1/tn3 inhibited granule cell migration. Localization of the immunoreactive domains by EM of rotary shadowed tenascin molecules revealed that the mAbs J1/tn4 and J1/tn5, like the previously described J1/tn1 antibody, bound between the third and fifth fibronectin type III homologous repeats and mAb J1/tn3 bound between the third and fifth EGF-like repeats. mAb J1/tn2 had previously been found to react between fibronectin type III homologous repeats 10 and 11 of the mouse molecule (Lochter, A., L. Vaughan, A. Kaplony, A. Prochiantz, M. Schachner, and A. Faissner. 1991. J. Cell Biol. 113:1159-1171). When postnatal granule cell neurons were cultured on tenascin adsorbed to polyornithine, both the percentage of neurite-bearing cells and the length of outgrowing neurites were increased when compared to neurons growing on polyornithine alone. This neurite outgrowth promoting effect of tenascin was abolished only by mAb J1/tn2 or tenascin added to the culture medium in soluble form. The other antibodies did not modify the stimulatory or inhibitory effects of the molecule. These observations indicate that tenascin influences neurite outgrowth and migration of cerebellar granule cells by different domains in the fibronectin type III homologous repeats.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Liao, H., Huang, W., Schachner, M., Guan, Y., Guo, J., Yan, J., Qin, J., Bai, X., Zhang, L. (2008). {beta} 1 Integrin-mediated Effects of Tenascin-R Domains EGFL and FN6-8 on Neural Stem/Progenitor Cell Proliferation and Differentiation in Vitro. J. Biol. Chem. 283: 27927-27936 [Abstract] [Full Text]
McManus, M. F., Golden, J. A. (2005). Topical Review: Neuronal Migration in Developmental Disorders. J Child Neurol 20: 280-286 [Abstract]
McManus, M. F., Golden, J. A. (2004). Topical Review: Neuronal Migration in Developmental Disorders. J Child Neurol 19: 280-286 [Abstract]
Rao, Y., Wong, K., Ward, M., Jurgensen, C., Wu, J. Y. (2002). Neuronal migration and molecular conservation with leukocyte chemotaxis. Genes Dev. 16: 2973-2984 [Full Text]
Evers, M. R., Salmen, B., Bukalo, O., Rollenhagen, A., Bosl, M. R., Morellini, F., Bartsch, U., Dityatev, A., Schachner, M. (2002). Impairment of L-type Ca2+ Channel-Dependent Forms of Hippocampal Synaptic Plasticity in Mice Deficient in the Extracellular Matrix Glycoprotein Tenascin-C. J. Neurosci. 22: 7177-7194 [Abstract] [Full Text]
Rigato, F., Garwood, J., Calco, V., Heck, N., Faivre-Sarrailh, C., Faissner, A. (2002). Tenascin-C Promotes Neurite Outgrowth of Embryonic Hippocampal Neurons through the Alternatively Spliced Fibronectin Type III BD Domains via Activation of the Cell Adhesion Molecule F3/Contactin. J. Neurosci. 22: 6596-6609 [Abstract] [Full Text]
Thelen, K., Kedar, V., Panicker, A. K., Schmid, R.-S., Midkiff, B. R., Maness, P. F. (2002). The Neural Cell Adhesion Molecule L1 Potentiates Integrin-Dependent Cell Migration to Extracellular Matrix Proteins. J. Neurosci. 22: 4918-4931 [Abstract] [Full Text]
Garcion, E., Faissner, A., ffrench-Constant, C. (2001). Knockout mice reveal a contribution of the extracellular matrix molecule tenascin-C to neural precursor proliferation and migration. Development 128: 2485-2496 [Abstract] [Full Text]
Maseruka, H., Ridgway, A., Tullo, A., Bonshek, R. (2000). Developmental Changes in Patterns of Expression of Tenascin-C Variants in the Human Cornea. IOVS 41: 4101-4107 [Abstract] [Full Text]
Heffron, D. S., Golden, J. A. (2000). DM-GRASP Is Necessary for Nonradial Cell Migration during Chick Diencephalic Development. J. Neurosci. 20: 2287-2294 [Abstract] [Full Text]
Vaillant, C., Didier-Bazes, M., Hutter, A., Belin, M.-F., Thomasset, N. (1999). Spatiotemporal Expression Patterns of Metalloproteinases and Their Inhibitors in the Postnatal Developing Rat Cerebellum. J. Neurosci. 19: 4994-5004 [Abstract] [Full Text]
Rauch, U., Clement, A., Retzler, C., Frohlich, L., Fassler, R., Gohring, W., Faissner, A. (1997). Mapping of a Defined Neurocan Binding Site to Distinct Domains of Tenascin-C. J. Biol. Chem. 272: 26905-26912 [Abstract] [Full Text]
Golden, J., Zitz, J., McFadden, K, Cepko, C. (1997). Cell migration in the developing chick diencephalon. Development 124: 3525-3533 [Abstract]
Fischer, D, Brown-Ludi, M, Schulthess, T, Chiquet-Ehrismann, R (1997). Concerted action of tenascin-C domains in cell adhesion, anti-adhesion and promotion of neurite outgrowth. J. Cell Sci. 110: 1513-1522 [Abstract]
Williamson, T., Gordon-Weeks, P.�R., Schachner, M., Taylor, J. (1996). Microtubule reorganization is obligatory for growth cone�turning. Proc. Natl. Acad. Sci. USA 93: 15221-15226 [Abstract] [Full Text]
Deryugina, E. I., Bourdon, M. A. (1996). Tenascin mediates human glioma cell migration and modulates cell migration on fibronectin. J. Cell Sci. 109: 643-652 [Abstract]
Mackie, E., Ramsey, S (1996). Modulation of osteoblast behaviour by tenascin. J. Cell Sci. 109: 1597-1604 [Abstract]
Fischer, D., Chiquet-Ehrismann, R., Bernasconi, C., Chiquet, M. (1995). A Single Heparin Binding Region within the Fibrinogen-like Domain Is Functional in Chick Tenascin-C. J. Biol. Chem. 270: 3378-3384 [Abstract] [Full Text]
Pesheva, P, Probstmeier, R, Skubitz, A., McCarthy, J., Furcht, L., Schachner, M (1994). Tenascin-R (J1 160/180 inhibits fibronectin-mediated cell adhesion--functional relatedness to tenascin-C. J. Cell Sci. 107: 2323-2333 [Abstract]
Wehrle-Haller, B, Chiquet, M (1993). Dual function of tenascin: simultaneous promotion of neurite growth and inhibition of glial migration. J. Cell Sci. 106: 597-610 [Abstract]
Sriramarao, P, Mendler, M, Bourdon, M. (1993). Endothelial cell attachment and spreading on human tenascin is mediated by alpha 2 beta 1 and alpha v beta 3 integrins. J. Cell Sci. 105: 1001-1012 [Abstract]
Saga, Y, Yagi, T, Ikawa, Y, Sakakura, T, Aizawa, S (1992). Mice develop normally without tenascin.. Genes Dev. 6: 1821-1831 [Abstract]