The integrin alpha 6 beta 4 is a laminin receptor

doi:10.1083/jcb.117.3.671

This Article

	Full Text (PDF, 1047K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Lee, E. C.
	Articles by Mercurio, A. M.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Lee, E. C.
	Articles by Mercurio, A. M.

Social Bookmarking

	What's this?

ARTICLES

The integrin alpha 6 beta 4 is a laminin receptor

EC Lee, MM Lotz, GD Steele Jr and AM Mercurio
Laboratory of Cancer Biology, Deaconess Hospital, Harvard Medical School, Boston, Massachusetts 02115.

In this study, the putative laminin receptor function of the alpha 6 beta 4 integrin was assessed. For this purpose, we used a human cell line, referred to as clone A, that was derived from a highly invasive, colon adenocarcinoma. This cell line, which expresses the alpha 6 beta 4 integrin, adheres to the E8 and not to the P1 fragment of laminin. The adhesion of clone A cells to laminin is extremely rapid with half- maximal adhesion observed at 5 min after plating. Adhesion to laminin is blocked by GoH3, and alpha 6 specific antibody (60% inhibition), as well as by A9, a beta 4 specific antibody (30% inhibition). Most importantly, we demonstrate that alpha 6 beta 4 binds specifically to laminin-Sepharose columns in the presence of either Mg2+ or Mn2+ and it is eluted from these columns with EDTA but not with NaCl. The alpha 6 beta 4 integrin does not bind to collagen-Sepharose, but the alpha 2 beta 1 integrin does bind. Clone A cells do not express alpha 6 beta 1 as evidenced by the following observations: (a) no beta 1 integrin is detected in beta 1 immunoblots of GoH3 immunoprecipitates; and (b) no alpha 6 beta 1 integrin is seen in GoH3 immunoprecipitates of clone A extracts that had been immunodepleted of all beta 4 containing integrin using the A9 antibody. These data establish that laminin is a ligand for the alpha 6 beta 4 integrin and that this integrin can function as a laminin receptor independently of alpha 6 beta 1.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Xuan, W., Li, Y.-J., Liu, G., Ben-David, Y., Archer, M. C. (2009). Interleukin-24 Induces Expression of {beta}4 Integrin but Suppresses Anchorage-Independent Growth of Rat Mammary Tumor Cells by a Mechanism That Is Independent of {beta}4. Mol Cancer Res 7: 433-442 [Abstract] [Full Text]
Gaudreault, M., Vigneault, F., Gingras, M.-E., Leclerc, S., Carrier, P., Germain, L., Guerin, S. L. (2008). Transcriptional Regulation of the Human {alpha}6 Integrin Gene by the Transcription Factor NFI during Corneal Wound Healing. IOVS 49: 3758-3767 [Abstract] [Full Text]
Gaudreault, M., Vigneault, F., Leclerc, S., Guerin, S. L. (2007). Laminin Reduces Expression of the Human {alpha}6 Integrin Subunit Gene by Altering the Level of the Transcription Factors Sp1 and Sp3. IOVS 48: 3490-3505 [Abstract] [Full Text]
Sroka, T. C., Pennington, M. E., Cress, A. E. (2006). Synthetic D-amino acid peptide inhibits tumor cell motility on laminin-5. Carcinogenesis 27: 1748-1757 [Abstract] [Full Text]
Amici, S. A., Dunn, W. A. Jr, Murphy, A. J., Adams, N. C., Gale, N. W., Valenzuela, D. M., Yancopoulos, G. D., Notterpek, L. (2006). Peripheral Myelin Protein 22 Is in Complex with {alpha}6beta4 Integrin, and Its Absence Alters the Schwann Cell Basal Lamina. J. Neurosci. 26: 1179-1189 [Abstract] [Full Text]
Chartier, N. T., Laine, M., Gout, S., Pawlak, G., Marie, C. A., Matos, P., Block, M. R., Jacquier-Sarlin, M. R. (2006). Laminin-5-integrin interaction signals through PI 3-kinase and Rac1b to promote assembly of adherens junctions in HT-29 cells. J. Cell Sci. 119: 31-46 [Abstract] [Full Text]
Adair-Kirk, T. L., Atkinson, J. J., Kelley, D. G., Arch, R. H., Miner, J. H., Senior, R. M. (2005). A Chemotactic Peptide from Laminin {alpha}5 Functions as a Regulator of Inflammatory Immune Responses via TNF{alpha}-mediated Signaling. J. Immunol. 174: 1621-1629 [Abstract] [Full Text]
Herlevsen, M., Schmidt, D.-S., Miyazaki, K., Zoller, M. (2003). The association of the tetraspanin D6.1A with the {alpha}6{beta}4 integrin supports cell motility and liver metastasis formation. J. Cell Sci. 116: 4373-4390 [Abstract] [Full Text]
Hiran, T. S., Mazurkiewicz, J. E., Kreienberg, P., Rice, F. L., LaFlamme, S. E. (2003). Endothelial expression of the {alpha}6{beta}4 integrin is negatively regulated during angiogenesis. J. Cell Sci. 116: 3771-3781 [Abstract] [Full Text]
Adair-Kirk, T. L., Atkinson, J. J., Broekelmann, T. J., Doi, M., Tryggvason, K., Miner, J. H., Mecham, R. P., Senior, R. M. (2003). A Site on Laminin {alpha}5, AQARSAASKVKVSMKF, Induces Inflammatory Cell Production of Matrix Metalloproteinase-9 and Chemotaxis. J. Immunol. 171: 398-406 [Abstract] [Full Text]
Whelan, M. C., Senger, D. R. (2003). Collagen I Initiates Endothelial Cell Morphogenesis by Inducing Actin Polymerization through Suppression of Cyclic AMP and Protein Kinase A. J. Biol. Chem. 278: 327-334 [Abstract] [Full Text]
Morena, A., Riccioni, S., Marchetti, A., Polcini, A. T., Mercurio, A. M., Blandino, G., Sacchi, A., Falcioni, R. (2002). Expression of the beta 4 integrin subunit induces monocytic differentiation of 32D/v-Abl cells. Blood 100: 96-106 [Abstract] [Full Text]
Davis, T. L., Buerger, F., Cress, A. E. (2002). Differential Regulation of a Novel Variant of the {alpha}6 Integrin, {alpha}6p. Cell Growth Differ. 13: 107-113 [Abstract] [Full Text]
Rabinovitz, I., Gipson, I. K., Mercurio, A. M. (2001). Traction Forces Mediated by alpha 6beta 4 Integrin: Implications for Basement Membrane Organization and Tumor Invasion. Mol. Biol. Cell 12: 4030-4043 [Abstract] [Full Text]
Shaw, L. M. (2001). Identification of Insulin Receptor Substrate 1 (IRS-1) and IRS-2 as Signaling Intermediates in the {alpha}6{beta}4 Integrin-Dependent Activation of Phosphoinositide 3-OH Kinase and Promotion of Invasion. Mol. Cell. Biol. 21: 5082-5093 [Abstract] [Full Text]
DeRoock, I. B., Pennington, M. E., Sroka, T. C., Lam, K. S., Bowden, G. T., Bair, E. L., Cress, A. E. (2001). Synthetic Peptides Inhibit Adhesion of Human Tumor Cells to Extracellular Matrix Proteins. Cancer Res. 61: 3308-3313 [Abstract] [Full Text]
Zhang, X. A., Bontrager, A. L., Stipp, C. S., Kraeft, S.-K., Bazzoni, G., Chen, L. B., Hemler, M. E. (2001). Phosphorylation of a Conserved Integrin {alpha}3 QPSXXE Motif Regulates Signaling, Motility, and Cytoskeletal Engagement. Mol. Biol. Cell 12: 351-365 [Abstract] [Full Text]
Gambaletta, D., Marchetti, A., Benedetti, L., Mercurio, A. M., Sacchi, A., Falcioni, R. (2000). Cooperative Signaling between alpha 6beta 4 Integrin and ErbB-2 Receptor Is Required to Promote Phosphatidylinositol 3-Kinase-dependent Invasion. J. Biol. Chem. 275: 10604-10610 [Abstract] [Full Text]
O'Connor, K. L., Nguyen, B.-K., Mercurio, A. M. (2000). Rhoa Function in Lamellae Formation and Migration Is Regulated by the {alpha}6{beta}4 Integrin and Camp Metabolism. JCB 148: 253-258 [Abstract] [Full Text]
Kimmins, S., MacLaren, L. A. (1999). Cyclic Modulation of Integrin Expression in Bovine Endometrium. Biol. Reprod. 61: 1267-1274 [Abstract] [Full Text]
Basora, N., Herring-Gillam, F. E., Boudreau, F., Perreault, N., Pageot, L.-P., Simoneau, M., Bouatrouss, Y., Beaulieu, J.-F. (1999). Expression of Functionally Distinct Variants of the beta 4A Integrin Subunit in Relation to the Differentiation State in Human Intestinal Cells. J. Biol. Chem. 274: 29819-29825 [Abstract] [Full Text]
Muschler, J., Lochter, A., Roskelley, C. D., Yurchenco, P., Bissell, M. J. (1999). Division of Labor among the alpha 6beta 4 Integrin, beta 1 Integrins, and an E3 Laminin Receptor to Signal Morphogenesis and beta -Casein Expression in Mammary Epithelial Cells. Mol. Biol. Cell 10: 2817-2828 [Abstract] [Full Text]
Montuori, N., Müller, F., De Riu, S., Fenzi, G., Sobel, M. E., Rossi, G., Vitale, M. (1999). Laminin Receptors in Differentiated Thyroid Tumors: Restricted Expression of the 67-Kilodalton Laminin Receptor in Follicular Carcinoma Cells. J. Clin. Endocrinol. Metab. 84: 2086-2092 [Abstract] [Full Text]
van der Neut, R, Cachaco, A., Thorsteinsdottir, S, Janssen, H, Prins, D, Bulthuis, J, van der Valk, M, Calafat, J, Sonnenberg, A (1999). Partial rescue of epithelial phenotype in integrin beta4 null mice by a keratin-5 promoter driven human integrin beta4 transgene. J. Cell Sci. 112: 3911-3922 [Abstract]
Takaoka, A. S., Yamada, T., Gotoh, M., Kanai, Y., Imai, K., Hirohashi, S. (1998). Cloning and Characterization of the Human beta 4-Integrin Gene Promoter and Enhancers. J. Biol. Chem. 273: 33848-33855 [Abstract] [Full Text]
Gimond, C., Baudoin, C., van der Neut, R., Kramer, D., Calafat, J., Sonnenberg, A. (1998). Cre-loxP-mediated Inactivation of the {alpha}6A Integrin Splice Variant In Vivo: Evidence for a Specific Functional Role of {alpha}6A in Lymphocyte Migration but Not in Heart Development. JCB 143: 253-266 [Abstract] [Full Text]
Katoh, K., Kano, Y., Masuda, M., Onishi, H., Fujiwara, K. (1998). Isolation and Contraction of the Stress Fiber. Mol. Biol. Cell 9: 1919-1938 [Abstract] [Full Text]
Hirako, Y., Usukura, J., Uematsu, J., Hashimoto, T., Kitajima, Y., Owaribe, K. (1998). Cleavage of BP180, a 180-kDa Bullous Pemphigoid Antigen, Yields a 120-kDa Collagenous Extracellular Polypeptide. J. Biol. Chem. 273: 9711-9717 [Abstract] [Full Text]
Rezniczek, G. A., de Pereda, J. M., Reipert, S., Wiche, G. (1998). Linking Integrin {alpha}6{beta}4-based Cell Adhesion to the Intermediate Filament Cytoskeleton: Direct Interaction between the {beta}4 Subunit and Plectin at Multiple Molecular Sites. JCB 141: 209-225 [Abstract] [Full Text]
Homan, S., Mercurio, A., LaFlamme, S. (1998). Endothelial cells assemble two distinct alpha6beta4-containing vimentin-associated structures: roles for ligand binding and the beta4 cytoplasmic tail. J. Cell Sci. 111: 2717-2728 [Abstract]
Rabinovitz, I., Mercurio, A. M. (1997). The Integrin {alpha}6{beta}4 Functions in Carcinoma Cell Migration on Laminin-1 by Mediating the Formation and Stabilization of Actin-containing Motility Structures. JCB 139: 1873-1884 [Abstract] [Full Text]
Biffo, S., Sanvito, F., Costa, S., Preve, L., Pignatelli, R., Spinardi, L., Marchisio, P. C. (1997). Isolation of a Novel beta 4 Integrin-binding Protein (p27BBP) Highly Expressed in Epithelial Cells. J. Biol. Chem. 272: 30314-30321 [Abstract] [Full Text]
Colognato, H., MacCarrick, M., O'Rear, J. J., Yurchenco, P. D. (1997). The Laminin alpha 2-Chain Short Arm Mediates Cell Adhesion through Both the alpha 1beta 1 and alpha 2beta 1 Integrins. J. Biol. Chem. 272: 29330-29336 [Abstract] [Full Text]
Desban, N, Duband, J. (1997). Avian neural crest cell migration on laminin: interaction of the alpha1beta1 integrin with distinct laminin-1 domains mediates different adhesive responses. J. Cell Sci. 110: 2729-2744 [Abstract]
Niessen, C., Hulsman, E., Oomen, L., Kuikman, I, Sonnenberg, A (1997). A minimal region on the integrin beta4 subunit that is critical to its localization in hemidesmosomes regulates the distribution of HD1/plectin in COS-7 cells. J. Cell Sci. 110: 1705-1716 [Abstract]
Hirako, Y., Usukura, J., Nishizawa, Y., Owaribe, K. (1996). Demonstration of the Molecular Shape of BP180, a 180-kDa Bullous Pemphigoid Antigen and Its Potential for Trimer Formation. J. Biol. Chem. 271: 13739-13745 [Abstract] [Full Text]
Chernousov, M. A., Stahl, R. C., Carey, D. J. (1996). Schwann Cells Secrete a Novel Collagen-like Adhesive Protein That Binds N-Syndecan. J. Biol. Chem. 271: 13844-13853 [Abstract] [Full Text]
Falk, M, Salmivirta, K, Durbeej, M, Larsson, E, Ekblom, M, Vestweber, D, Ekblom, P (1996). Integrin alpha 6B beta 1 is involved in kidney tubulogenesis in vitro. J. Cell Sci. 109: 2801-2810 [Abstract]
Lallier, T., Whittaker, C., DeSimone, D. (1996). Integrin alpha 6 expression is required for early nervous system development in Xenopus laevis. Development 122: 2539-2554 [Abstract]
Niessen, C., van der Raaij-Helmer, M., Hulsman, E., van der Neut, R, Jonkman, M., Sonnenberg, A (1996). Deficiency of the integrin beta 4 subunit in junctional epidermolysis bullosa with pyloric atresia: consequences for hemidesmosome formation and adhesion properties. J. Cell Sci. 109: 1695-1706 [Abstract]
Giancotti, F. (1996). Signal transduction by the alpha 6 beta 4 integrin: charting the path between laminin binding and nuclear events. J. Cell Sci. 109: 1165-1172
Clarke, A. S., Lotz, M. M., Chao, C., Mercurio, A. M. (1995). Activation of the p21 Pathway of Growth Arrest and Apoptosis by the beta(4) Integrin Cytoplasmic Domain. J. Biol. Chem. 270: 22673-22676 [Abstract] [Full Text]
Gaietta, G, Redelmeier, T., Jackson, M., Tamura, R., Quaranta, V (1994). Quantitative measurement of alpha 6 beta 1 and alpha 6 beta 4 integrin internalization under cross-linking conditions: a possible role for alpha 6 cytoplasmic domains. J. Cell Sci. 107: 3339-3349 [Abstract]
Tozeren, A, Kleinman, H., Wu, S, Mercurio, A., Byers, S. (1994). Integrin alpha 6 beta 4 mediates dynamic interactions with laminin. J. Cell Sci. 107: 3153-3163 [Abstract]
Feltri, M., Scherer, S., Nemni, R, Kamholz, J, Vogelbacker, H, Scott, M., Canal, N, Quaranta, V, Wrabetz, L (1994). Beta 4 integrin expression in myelinating Schwann cells is polarized, developmentally regulated and axonally dependent. Development 120: 1287-1301 [Abstract]
Ojakian, G., Schwimmer, R (1994). Regulation of epithelial cell surface polarity reversal by beta 1 integrins. J. Cell Sci. 107: 561-576 [Abstract]
Simon-Assmann, P, Leberquier, C, Molto, N, Uezato, T, Bouziges, F, Kedinger, M (1994). Adhesive properties and integrin expression profiles of two colonic cancer populations differing by their spreading on laminin. J. Cell Sci. 107: 577-587 [Abstract]
Schoenenberger, C., Zuk, A, Zinkl, G., Kendall, D, Matlin, K. (1994). Integrin expression and localization in normal MDCK cells and transformed MDCK cells lacking apical polarity. J. Cell Sci. 107: 527-541 [Abstract]
Niessen, C., Cremona, O, Daams, H, Ferraresi, S, Sonnenberg, A, Marchisio, P. (1994). Expression of the integrin alpha 6 beta 4 in peripheral nerves: localization in Schwann and perineural cells and different variants of the beta 4 subunit. J. Cell Sci. 107: 543-552 [Abstract]
Sonnenberg, A, de Melker, A., Martinez de Velasco, A., Janssen, H, Calafat, J, Niessen, C. (1993). Formation of hemidesmosomes in cells of a transformed murine mammary tumor cell line and mechanisms involved in adherence of these cells to laminin and kalinin. J. Cell Sci. 106: 1083-1102 [Abstract]
Aplin, J. D., Sattar, A., Mould, A. P. (1992). Variant choriocarcinoma (BeWo) cells that differ in adhesion and migration on fibronectin display conserved patterns of integrin expression. J. Cell Sci. 103: 435-444 [Abstract]
Lallier, T, Leblanc, G, Artinger, K., Bronner-Fraser, M (1992). Cranial and trunk neural crest cells use different mechanisms for attachment to extracellular matrices. Development 116: 531-541 [Abstract]
Tani, T. T., Mercurio, A. M. (2001). PDZ Interaction Sites in Integrin alpha Subunits. TIP-2/GIPC BINDS TO A TYPE I RECOGNITION SEQUENCE IN alpha 6A/alpha 5 AND A NOVEL SEQUENCE IN alpha 6B. J. Biol. Chem. 276: 36535-36542 [Abstract] [Full Text]