Expression of a human hepatocyte growth factor/scatter factor cDNA in MDCK epithelial cells influences cell morphology, motility, and anchorage-independent growth

doi:10.1083/jcb.117.4.889

This Article

	Full Text (PDF, 1547K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Uehara, Y.
	Articles by Kitamura, N.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Uehara, Y.
	Articles by Kitamura, N.


Social Bookmarking

	What's this?

ARTICLES

Expression of a human hepatocyte growth factor/scatter factor cDNA in MDCK epithelial cells influences cell morphology, motility, and anchorage-independent growth

Y Uehara and N Kitamura
Institute for Liver Research, Kansai Medical University, Osaka, Japan.

The addition of exogenous hepatocyte growth factor (HGF)/scatter factor (SF) to MDCK epithelial cells results in fibroblastic morphology and cell motility. We generated HGF/SF producing MDCK cells by transfection with an expression plasmid containing human HGF/SF cDNA. Production of HGF/SF by these cells induced a change from an epithelial to a fibroblastic morphology and increased cell motility. In addition, the HGF/SF producing cells acquired efficient anchorage-independent growth in soft agar but did not form tumors in nude mice. The morphological change and the stimulation of the anchorage-independent growth were prevented by anti-HGF/SF antibody, suggesting that the factor is secreted and then exerts its effects through cell surface receptors.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Klymkowsky, M. W., Savagner, P. (2009). Epithelial-Mesenchymal Transition: A Cancer Researcher's Conceptual Friend and Foe. Am. J. Pathol. 174: 1588-1593 [Abstract] [Full Text]
Wu, J.-C., Chen, T.-Y., Yu, C.-T. R., Tsai, S.-J., Hsu, J.-M., Tang, M.-J., Chou, C.-K., Lin, W.-J., Yuan, C.-J., Huang, C.-Y. F. (2005). Identification of V23RalA-Ser194 as a Critical Mediator for Aurora-A-induced Cellular Motility and Transformation by Small Pool Expression Screening. J. Biol. Chem. 280: 9013-9022 [Abstract] [Full Text]
Zoltan-Jones, A., Huang, L., Ghatak, S., Toole, B. P. (2003). Elevated Hyaluronan Production Induces Mesenchymal and Transformed Properties in Epithelial Cells. J. Biol. Chem. 278: 45801-45810 [Abstract] [Full Text]
Rosenthal, E. L., Wax, M. K., Anderson, P., Kulecz-Martin, M. (2001). The Mucosal Invasion Model: A Novel In Vitro Model for Evaluating the Invasive Behavior of Mucocutaneous Malignancies. Arch Otolaryngol Head Neck Surg 127: 1467-1470 [Abstract] [Full Text]
He, Z., Bateman, A. (1999). Progranulin Gene Expression Regulates Epithelial Cell Growth and Promotes Tumor Growth in Vivo. Cancer Res. 59: 3222-3229 [Abstract] [Full Text]
Bell, A. W., Jiang, J.-G., Chen, Q., Liu, Y., Zarnegar, R. (1998). The Upstream Regulatory Regions of the Hepatocyte Growth Factor Gene Promoter Are Essential for Its Expression in Transgenic Mice. J. Biol. Chem. 273: 6900-6908 [Abstract] [Full Text]
Skouteris, G., Schroder, C. (1997). Cytosolic phospholipase A2 is activated by the hepatocyte growth factor receptor-kinase in Madin Darby canine kidney cells. J. Cell Sci. 110: 1655-1663 [Abstract]
Clark, P (1994). Modulation of scatter factor/hepatocyte growth factor activity by cell-substratum adhesion. J. Cell Sci. 107: 1265-1275 [Abstract]