Requirement for p34cdc2 kinase is restricted to mitosis in the mammalian cdc2 mutant FT210

doi:10.1083/jcb.117.5.1041

This Article

	Full Text (PDF, 2000K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Hamaguchi, J. R.
	Articles by Bradbury, E. M.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Hamaguchi, J. R.
	Articles by Bradbury, E. M.


Social Bookmarking

	What's this?

ARTICLES

Requirement for p34cdc2 kinase is restricted to mitosis in the mammalian cdc2 mutant FT210

JR Hamaguchi, RA Tobey, J Pines, HA Crissman, T Hunter and EM Bradbury
Department of Biological Chemistry, School of Medicine, University of California, Davis 95616.

The mouse FT210 cell line is a temperature-sensitive cdc2 mutant. FT210 cells are found to arrest specifically in G2 phase and unlike many alleles of cdc2 and cdc28 mutants of yeasts, loss of p34cdc2 at the nonpermissive temperature has no apparent effect on cell cycle progression through the G1 and S phases of the division cycle. FT210 cells and the parent wild-type FM3A cell line each possess at least three distinct histone H1 kinases. H1 kinase activities in chromatography fractions were identified using a synthetic peptide substrate containing the consensus phosphorylation site of histone H1 and the kinase subunit compositions were determined immunochemically with antisera prepared against the "PSTAIR" peptide, the COOH-terminus of mammalian p34cdc2 and the human cyclins A and B1. The results show that p34cdc2 forms two separate complexes with cyclin A and with cyclin B1, both of which exhibit thermal lability at the non-permissive temperature in vitro and in vivo. A third H1 kinase with stable activity at the nonpermissive temperature is comprised of cyclin A and a cdc2-like 34-kD subunit, which is immunoreactive with anti-"PSTAIR" antiserum but is not recognized with antiserum specific for the COOH- terminus of p34cdc2. The cyclin A-associated kinases are active during S and G2 phases and earlier in the division cycle than the p34cdc2- cyclin B1 kinase. We show that mouse cells possess at least two cdc2- related gene products which form cell cycle regulated histone H1 kinases and we propose that the murine homolog of yeast p34cdc/CDC28 is essential only during the G2-to-M transition in FT210 cells.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Ullah, Z., Kohn, M. J., Yagi, R., Vassilev, L. T., DePamphilis, M. L. (2008). Differentiation of trophoblast stem cells into giant cells is triggered by p57/Kip2 inhibition of CDK1 activity. Genes Dev. 22: 3024-3036 [Abstract] [Full Text]
Li, C.-j., Vassilev, A., DePamphilis, M. L. (2004). Role for Cdk1 (Cdc2)/Cyclin A in Preventing the Mammalian Origin Recognition Complex's Largest Subunit (Orc1) from Binding to Chromatin during Mitosis. Mol. Cell. Biol. 24: 5875-5886 [Abstract] [Full Text]
Sugimoto, N., Tatsumi, Y., Tsurumi, T., Matsukage, A., Kiyono, T., Nishitani, H., Fujita, M. (2004). Cdt1 Phosphorylation by Cyclin A-dependent Kinases Negatively Regulates Its Function without Affecting Geminin Binding. J. Biol. Chem. 279: 19691-19697 [Abstract] [Full Text]
Li, T., Inoue, A., Lahti, J. M., Kidd, V. J. (2004). Failure To Proliferate and Mitotic Arrest of CDK11p110/p58-Null Mutant Mice at the Blastocyst Stage of Embryonic Cell Development. Mol. Cell. Biol. 24: 3188-3197 [Abstract] [Full Text]
Fujita, M., Yamada, C., Tsurumi, T., Hanaoka, F., Matsuzawa, K., Inagaki, M. (1998). Cell Cycle- and Chromatin Binding State-dependent Phosphorylation of Human MCM Heterohexameric Complexes. A ROLE FOR cdc2 KINASE. J. Biol. Chem. 273: 17095-17101 [Abstract] [Full Text]
Gabrielli, B., De Souza, C., Tonks, I., Clark, J., Hayward, N., Ellem, K. (1996). Cytoplasmic accumulation of cdc25B phosphatase in mitosis triggers centrosomal microtubule nucleation in HeLa cells. J. Cell Sci. 109: 1081-1093 [Abstract]
Sauer, K, Knoblich, J A, Richardson, H, Lehner, C F (1995). Distinct modes of cyclin E/cdc2c kinase regulation and S-phase control in mitotic and endoreduplication cycles of Drosophila embryogenesis.. Genes Dev. 9: 1327-1339 [Abstract]
Ongkeko, W, Ferguson, D., Harris, A., Norbury, C (1995). Inactivation of Cdc2 increases the level of apoptosis induced by DNA damage. J. Cell Sci. 108: 2897-2904 [Abstract]
Chevalier, S, Tassan, J., Cox, R, Philippe, M, Ford, C (1995). Both cdc2 and cdk2 promote S phase initiation in Xenopus egg extracts. J. Cell Sci. 108: 1831-1841 [Abstract]
Creanor, J, Mitchison, J. (1994). The kinetics of H1 histone kinase activation during the cell cycle of wild-type and wee mutants of the fission yeast Schizosaccharomyces pombe. J. Cell Sci. 107: 1197-1204 [Abstract]
Tsai, L. H., Takahashi, T., Caviness, V. S., Harlow, E. (1993). Activity and expression pattern of cyclin-dependent kinase 5 in the embryonic mouse nervous system. Development 119: 1029-1040 [Abstract]
Baldin, V, Lukas, J, Marcote, M J, Pagano, M, Draetta, G (1993). Cyclin D1 is a nuclear protein required for cell cycle progression in G1.. Genes Dev. 7: 812-821 [Abstract]
Stern, B., Ried, G., Clegg, N. J., Grigliatti, T. A., Lehner, C. F. (1993). Genetic analysis of the Drosophila cdc2 homolog. Development 117: 219-232 [Abstract]