Purification, characterization, and immunofluorescence localization of Saccharomyces cerevisiae capping protein

doi:10.1083/jcb.117.5.1067

This Article

	Full Text (PDF, 3117K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Amatruda, J. F.
	Articles by Cooper, J. A.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Amatruda, J. F.
	Articles by Cooper, J. A.


Social Bookmarking

	What's this?

ARTICLES

Purification, characterization, and immunofluorescence localization of Saccharomyces cerevisiae capping protein

JF Amatruda and JA Cooper
Department of Cell Biology and Physiology, Washington University School of Medicine, St. Louis, Missouri 63110.

Capping protein binds the barbed ends of actin filaments and nucleates actin filament assembly in vitro. We purified capping protein from Saccharomyces cervisiae. One of the two subunits is the product of the CAP2 gene, which we previously identified as the gene encoding the beta subunit of capping protein based on its sequence similarity to capping protein beta subunits in chicken and Dictyostelium (Amatruda, J. F., J. F. Cannon, K. Tatchell, C. Hug, and J. A. Cooper. 1990. Nature (Lond.) 344:352-354). Yeast capping protein has activity in critical concentration and low-shear viscometry assays consistent with barbed- end capping activity. Like chicken capping protein, yeast capping protein is inhibited by PIP2. By immunofluorescence microscopy yeast capping protein colocalizes with cortical actin spots at the site of bud emergence and at the tips of growing buds and shmoos. In contrast, capping protein does not colocalize with actin cables or with actin rings at the site of cytokinesis.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Kuhn, J. R., Pollard, T. D. (2007). Single Molecule Kinetic Analysis of Actin Filament Capping: POLYPHOSPHOINOSITIDES DO NOT DISSOCIATE CAPPING PROTEINS. J. Biol. Chem. 282: 28014-28024 [Abstract] [Full Text]
Buttery, S. M., Yoshida, S., Pellman, D. (2007). Yeast Formins Bni1 and Bnr1 Utilize Different Modes of Cortical Interaction during the Assembly of Actin Cables. Mol. Biol. Cell 18: 1826-1838 [Abstract] [Full Text]
Park, H.-O., Bi, E. (2007). Central Roles of Small GTPases in the Development of Cell Polarity in Yeast and Beyond. Microbiol. Mol. Biol. Rev. 71: 48-96 [Abstract] [Full Text]
Smythe, E., Ayscough, K. R. (2006). Actin regulation in endocytosis. J. Cell Sci. 119: 4589-4598 [Abstract] [Full Text]
Sagot, I., Pinson, B., Salin, B., Daignan-Fornier, B. (2006). Actin Bodies in Yeast Quiescent Cells: An Immediately Available Actin Reserve?. Mol. Biol. Cell 17: 4645-4655 [Abstract] [Full Text]
Moseley, J. B., Goode, B. L. (2006). The Yeast Actin Cytoskeleton: from Cellular Function to Biochemical Mechanism. Microbiol. Mol. Biol. Rev. 70: 605-645 [Abstract] [Full Text]
Frank, D. J., Hopmann, R., Lenartowska, M., Miller, K. G. (2006). Capping Protein and the Arp2/3 Complex Regulate Nonbundle Actin Filament Assembly to Indirectly Control Actin Bundle Positioning during Drosophila melanogaster Bristle Development. Mol. Biol. Cell 17: 3930-3939 [Abstract] [Full Text]
Moseley, J. B., Okada, K., Balcer, H. I., Kovar, D. R., Pollard, T. D., Goode, B. L. (2006). Twinfilin is an actin-filament-severing protein and promotes rapid turnover of actin structures in vivo. J. Cell Sci. 119: 1547-1557 [Abstract] [Full Text]
Huang, S., Gao, L., Blanchoin, L., Staiger, C. J. (2006). Heterodimeric Capping Protein from Arabidopsis Is Regulated by Phosphatidic Acid. Mol. Biol. Cell 17: 1946-1958 [Abstract] [Full Text]
Moseley, J. B., Goode, B. L. (2005). Differential Activities and Regulation of Saccharomyces cerevisiae Formin Proteins Bni1 and Bnr1 by Bud6. J. Biol. Chem. 280: 28023-28033 [Abstract] [Full Text]
Kovar, D. R., Wu, J.-Q., Pollard, T. D. (2005). Profilin-mediated Competition between Capping Protein and Formin Cdc12p during Cytokinesis in Fission Yeast. Mol. Biol. Cell 16: 2313-2324 [Abstract] [Full Text]
Mattila, P. K., Quintero-Monzon, O., Kugler, J., Moseley, J. B., Almo, S. C., Lappalainen, P., Goode, B. L. (2004). A High-affinity Interaction with ADP-Actin Monomers Underlies the Mechanism and In Vivo Function of Srv2/cyclase-associated Protein. Mol. Biol. Cell 15: 5158-5171 [Abstract] [Full Text]
Kim, K., Yamashita, A., Wear, M. A., Maeda, Y., Cooper, J. A. (2004). Capping protein binding to actin in yeast: biochemical mechanism and physiological relevance. JCB 164: 567-580 [Abstract] [Full Text]
Moseley, J. B., Sagot, I., Manning, A. L., Xu, Y., Eck, M. J., Pellman, D., Goode, B. L. (2004). A Conserved Mechanism for Bni1- and mDia1-induced Actin Assembly and Dual Regulation of Bni1 by Bud6 and Profilin. Mol. Biol. Cell 15: 896-907 [Abstract] [Full Text]
Huang, S., Blanchoin, L., Kovar, D. R., Staiger, C. J. (2003). Arabidopsis Capping Protein (AtCP) Is a Heterodimer That Regulates Assembly at the Barbed Ends of Actin Filaments. J. Biol. Chem. 278: 44832-44842 [Abstract] [Full Text]
Young, M. E., Karpova, T. S., Brugger, B., Moschenross, D. M., Wang, G. K., Schneiter, R., Wieland, F. T., Cooper, J. A. (2002). The Sur7p Family Defines Novel Cortical Domains in Saccharomyces cerevisiae, Affects Sphingolipid Metabolism, and Is Involved in Sporulation. Mol. Cell. Biol. 22: 927-934 [Abstract] [Full Text]
Drees, B. L., Sundin, B., Brazeau, E., Caviston, J. P., Chen, G.-C., Guo, W., Kozminski, K. G., Lau, M. W., Moskow, J. J., Tong, A., Schenkman, L. R., McKenzie, A. III, Brennwald, P., Longtine, M., Bi, E., Chan, C., Novick, P., Boone, C., Pringle, J. R., Davis, T. N., Fields, S., Drubin, D. G. (2001). A protein interaction map for cell polarity development. JCB 154: 549-576 [Abstract] [Full Text]
Ni, L., Snyder, M. (2001). A Genomic Study of the Bipolar Bud Site Selection Pattern in Saccharomyces cerevisiae. Mol. Biol. Cell 12: 2147-2170 [Abstract] [Full Text]
Adames, N. R., Oberle, J. R., Cooper, J. A. (2001). The Surveillance Mechanism of the Spindle Position Checkpoint in Yeast. JCB 153: 159-168 [Abstract] [Full Text]
Whitacre, J. L., Davis, D. A., Toenjes, K. A., Brower, S. M., Adams, A. E. M. (2001). Generation of an Isogenic Collection of Yeast Actin Mutants and Identification of Three Interrelated Phenotypes. Genetics 157: 533-543 [Abstract] [Full Text]
Ooms, L. M., McColl, B. K., Wiradjaja, F., Wijayaratnam, A. P. W., Gleeson, P., Gething, M. J., Sambrook, J., Mitchell, C. A. (2000). The Yeast Inositol Polyphosphate 5-Phosphatases Inp52p and Inp53p Translocate to Actin Patches following Hyperosmotic Stress: Mechanism for Regulating Phosphatidylinositol 4,5-Bisphosphate at Plasma Membrane Invaginations. Mol. Cell. Biol. 20: 9376-9390 [Abstract] [Full Text]
Bi, E., Chiavetta, J. B., Chen, H., Chen, G.-C., Chan, C. S. M., Pringle, J. R. (2000). Identification of Novel, Evolutionarily Conserved Cdc42p-interacting Proteins and of Redundant Pathways Linking Cdc24p and Cdc42p to Actin Polarization in Yeast. Mol. Biol. Cell 11: 773-793 [Abstract] [Full Text]
Pruyne, D, Bretscher, A (2000). Polarization of cell growth in yeast. J. Cell Sci. 113: 571-585 [Abstract]
Morrell, J. L., Morphew, M., Gould, K. L. (1999). A Mutant of Arp2p Causes Partial Disassembly of the Arp2/3 Complex and Loss of Cortical Actin Function in Fission Yeast. Mol. Biol. Cell 10: 4201-4215 [Abstract] [Full Text]
Winter, D. C., Choe, E. Y., Li, R. (1999). Genetic dissection of the budding yeast Arp2/3 complex: A comparison of the in vivo and structural roles of individual subunits. Proc. Natl. Acad. Sci. USA 96: 7288-7293 [Abstract] [Full Text]
Lau, P.-m., Zucker, R. S., Bentley, D. (1999). Induction of Filopodia by Direct Local Elevation of Intracellular Calcium Ion Concentration. JCB 145: 1265-1276 [Abstract] [Full Text]
Arkowitz, R. A., Lowe, N. (1997). A Small Conserved Domain in the Yeast Spa2p Is Necessary and Sufficient for Its Polarized Localization. JCB 138: 17-36 [Abstract] [Full Text]
Li, R. (1997). Bee1, a Yeast Protein with Homology to Wiscott-Aldrich Syndrome Protein, Is Critical for the Assembly of Cortical Actin Cytoskeleton. JCB 136: 649-658 [Abstract] [Full Text]
Barron-Casella, E. A., Torres, M. A., Scherer, S. W., Heng, H. H. Q., Tsui, L.-C., Casella, J. F. (1995). Sequence Analysis and Chromosomal Localization of Human Cap Z. J. Biol. Chem. 270: 21472-21479 [Abstract] [Full Text]
Ivanenkov, V. V., Jamieson, G. A. Jr., Gruenstein, E., Dimlich, R. V. W. (1995). Characterization of S-100b Binding Epitopes. J. Biol. Chem. 270: 14651-14658 [Abstract] [Full Text]
Pringle, J.R., Bi, E., Harkins, H.A., Zahner, J.E., De Virgilio, C., Chant, J., Corrado, K., Fares, H. (1995). Establishment of Cell Polarity in Yeast. Cold Spring Harb Symp Quant Biol 60: 729-744 [Abstract]