The Journal of Cell Biology, Vol 117, 1109-1117, Copyright © 1992 by The Rockefeller University Press

ARTICLES

Regulation of alpha 2 beta 1-mediated fibroblast migration on type I collagen by shifts in the concentrations of extracellular Mg2+ and Ca2+ [published erratum appears in J Cell Biol 1992 Jul;118(1):219]

JJ Grzesiak, GE Davis, D Kirchhofer and MD Pierschbacher
La Jolla Cancer Research Foundation, California 92037.

Extracellular Ca2+ can reverse the Mg(2+)-dependent, alpha 2 beta 1- mediated adhesion of WI38 human fibroblasts to type I collagen substrates. Affinity chromatography data also demonstrate that Ca2+ can specifically elute the fibroblast alpha 2 beta 1 integrin bound to type I collagen-Sepharose in Mg2+. In modified Boyden chamber migration assays, Mg2+ alone supports the alpha 2 beta 1-mediated migration of fibroblasts on type I collagen substrates, while Ca2+ does not. However, a twofold enhancement in migration was observed when combinations of the two cations were used, with optimal migration observed when the Mg2+/Ca2+ ratio was higher than one. Inhibitory mAbs directed against various integrin subunits demonstrate that these observed cation effects appear to be mediated primarily by alpha 2 beta 1. These data, together with reports that under certain physiological conditions significant fluctuations in the concentrations of extracellular Ca2+ and Mg2+ can take place in vivo, suggest that the ratio between these two cations is involved in the up- and downregulation of integrin function, and thus, may influence cell migratory behavior.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Facebook    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

• Valdramidou, D., Humphries, M. J., Mould, A. P. (2008). Distinct Roles of {beta}1 Metal Ion-dependent Adhesion Site (MIDAS), Adjacent to MIDAS (ADMIDAS), and Ligand-associated Metal-binding Site (LIMBS) Cation-binding Sites in Ligand Recognition by Integrin {alpha}2{beta}1. J. Biol. Chem. 283: 32704-32714 [Abstract] [Full Text]  
• Ajroud, K., Sugimori, T., Goldmann, W. H., Fathallah, D. M., Xiong, J.-P., Arnaout, M. A. (2004). Binding Affinity of Metal Ions to the CD11b A-domain Is Regulated by Integrin Activation and Ligands. J. Biol. Chem. 279: 25483-25488 [Abstract] [Full Text]  
• Siljander, P., Lassila, R. (1999). Studies of Adhesion-Dependent Platelet Activation : Distinct Roles for Different Participating Receptors Can Be Dissociated by Proteolysis of Collagen. Arterioscler. Thromb. Vasc. Bio. 19: 3033-3043 [Abstract] [Full Text]  
• Cattelino, A., Albertinazzi, C., Bossi, M., Critchley, D. R., de Curtis, I. (1999). A Cell-free System to Study Regulation of Focal Adhesions and of the Connected Actin Cytoskeleton. Mol. Biol. Cell 10: 373-391 [Abstract] [Full Text]  
• Crawford, J. R., Jacobson, B. S. (1998). Extracellular Calcium Regulates HeLa Cell Morphology during Adhesion to Gelatin: Role of Translocation and Phosphorylation of Cytosolic Phospholipase A2. Mol. Biol. Cell 9: 3429-3443 [Abstract] [Full Text]  
• LaFleur, D. W., Chiang, J., Fagin, J. A., Schwartz, S. M., Shah, P. K., Wallner, K., Forrester, J. S., Sharifi, B. G. (1997). Aortic Smooth Muscle Cells Interact with Tenascin-C through Its Fibrinogen-like Domain. J. Biol. Chem. 272: 32798-32803 [Abstract] [Full Text]  
• Ho, W.-c., Heinemann, C., Hangan, D., Uniyal, S., Morris, V. L., Chan, B. M.C. (1997). Modulation of In Vivo Migratory Function of alpha 2beta 1 Integrin in Mouse Liver. Mol. Biol. Cell 8: 1863-1875 [Abstract] [Full Text]  
• Lin, E. C. K., Ratnikov, B. I., Tsai, P. M., Gonzalez, E. R., McDonald, S., Pelletier, A. J., Smith, J. W. (1997). Evidence That the Integrin beta 3 and beta 5 Subunits Contain a Metal Ion-dependent Adhesion Site-like Motif but Lack an I Domain. J. Biol. Chem. 272: 14236-14243 [Abstract] [Full Text]  
• Veiga, S. S., Elias, M. C. Q. B., Gremski, W., Porcionatto, M. A., da Silva, R., Nader, H. B., Brentani, R. R. (1997). Post-translational Modifications of alpha 5beta 1 Integrin by Glycosaminoglycan Chains. THE alpha 5beta 1 INTEGRIN IS A FACULTATIVE PROTEOGLYCAN. J. Biol. Chem. 272: 12529-12535 [Abstract] [Full Text]  
• Luque, A., Gómez, M., Puzon, W., Takada, Y., Sánchez-Madrid, F., Cabañas, C. (1996). Activated Conformations of Very Late Activation Integrins Detected by a Group of Antibodies (HUTS) Specific for a Novel Regulatory Region(355-425) of the Common beta1 Chain. J. Biol. Chem. 271: 11067-11075 [Abstract] [Full Text]  
• Deryugina, E. I., Bourdon, M. A. (1996). Tenascin mediates human glioma cell migration and modulates cell migration on fibronectin. J. Cell Sci. 109: 643-652 [Abstract]  
• Ruggiero, F, Comte, J, Cabanas, C, Garrone, R (1996). Structural requirements for alpha 1 beta 1 and alpha 2 beta 1 integrin mediated cell adhesion to collagen V. J. Cell Sci. 109: 1865-1874 [Abstract]  
• Bazzoni, G., Shih, D.-T., Buck, C. A., Hemler, M. E. (1995). Monoclonal Antibody 9EG7 Defines a Novel beta(1) Integrin Epitope Induced by Soluble Ligand and Manganese, but Inhibited by Calcium. J. Biol. Chem. 270: 25570-25577 [Abstract] [Full Text]  
• Tuckwell, D, Calderwood, D., Green, L., Humphries, M. (1995). Integrin alpha 2 I-domain is a binding site for collagens. J. Cell Sci. 108: 1629-1637 [Abstract]  
• Keely, P., Fong, A., Zutter, M., Santoro, S. (1995). Alteration of collagen-dependent adhesion, motility, and morphogenesis by the expression of antisense alpha 2 integrin mRNA in mammary cells. J. Cell Sci. 108: 595-607 [Abstract]  
• Fabre, M, Garcia de Herreros, A (1993). Phorbol ester-induced scattering of HT-29 human intestinal cancer cells is associated with down-modulation of E-cadherin. J. Cell Sci. 106: 513-521 [Abstract]  

Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents