The Journal of Cell Biology, Vol 117, 935-948, Copyright © 1992 by The Rockefeller University Press

ARTICLES

Localization of RAP1 and topoisomerase II in nuclei and meiotic chromosomes of yeast

F Klein, T Laroche, ME Cardenas, JF Hofmann, D Schweizer and SM Gasser
Swiss Institute for Experimental Cancer Research (ISREC), Epalinges s/Lausanne.

Topoisomerase II (topoII) and RAP1 (Repressor Activator Protein 1) are two abundant nuclear proteins with proposed structural roles in the higher-order organization of chromosomes. Both proteins co-fractionate as components of nuclear scaffolds from vegetatively growing yeast cells, and both proteins are present as components of pachytene chromosome, co-fractionating with an insoluble subfraction of meiotic nuclei. Immunolocalization using antibodies specific for topoII shows staining of an axial core of the yeast meiotic chromosome, extending the length of the synaptonemal complex. RAP1, on the other hand, is located at the ends of the paired bivalent chromosomes, consistent with its ability to bind telomeric sequences in vitro. In interphase nuclei, again in contrast to anti-topoII, anti-RAP1 gives a distinctly punctate staining that is located primarily at the nuclear periphery. Approximately 16 brightly staining foci can be identified in a diploid nucleus stained with anti-RAP1 antibodies, suggesting that telomeres are grouped together, perhaps through interaction with the nuclear envelope.
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• Tomaska, L'u., Nosek, J., Fukuhara, H. (1997). Identification of a Putative Mitochondrial Telomere-binding Protein of the Yeast Candida parapsilosis. J. Biol. Chem. 272: 3049-3056 [Abstract] [Full Text]  
• Andreassen, P. R., Lacroix, F. B., Margolis, R. L. (1997). Chromosomes with Two Intact Axial Cores Are Induced by G2 Checkpoint Override: Evidence That DNA Decatenation Is not Required to Template the Chromosome Structure. JCB 136: 29-43 [Abstract] [Full Text]  
• Maillet, L, Boscheron, C, Gotta, M, Marcand, S, Gilson, E, Gasser, S M (1996). Evidence for silencing compartments within the yeast nucleus: a role for telomere proximity and Sir protein concentration in silencer-mediated repression.. Genes Dev. 10: 1796-1811 [Abstract]  
• Friedman, K L, Diller, J D, Ferguson, B M, Nyland, S V, Brewer, B J, Fangman, W L (1996). Multiple determinants controlling activation of yeast replication origins late in S phase.. Genes Dev. 10: 1595-1607 [Abstract]  
• Marcand, S, Buck, S W, Moretti, P, Gilson, E, Shore, D (1996). Silencing of genes at nontelomeric sites in yeast is controlled by sequestration of silencing factors at telomeres by Rap 1 protein.. Genes Dev. 10: 1297-1309 [Abstract]  
• Holmes, S G, Broach, J R (1996). Silencers are required for inheritance of the repressed state in yeast.. Genes Dev. 10: 1021-1032 [Abstract]  
• Chong, L., van Steensel, B., Broccoli, D., Erdjument-Bromage, H., Hanish, J., Tempst, P., de Lange, T. (1995). A Human Telomeric Protein. Science 270: 1663-1667 [Abstract]  
• Gromova, I. I., Nielsen, O. F., Razin, S. V. (1995). Long-range Fragmentation of the Eukaryotic Genome by Exogenous and Endogenous Nucleases Proceeds in a Specific Fashion via Preferential DNA Cleavage at Matrix Attachment Sites. J. Biol. Chem. 270: 18685-18690 [Abstract] [Full Text]  
• Allshire, R C, Nimmo, E R, Ekwall, K, Javerzat, J P, Cranston, G (1995). Mutations derepressing silent centromeric domains in fission yeast disrupt chromosome segregation.. Genes Dev. 9: 218-233 [Abstract]  
• Meller, V., Fisher, P. (1995). Nuclear distribution of Drosophila DNA topoisomerase II is sensitive to both RNase and DNase. J. Cell Sci. 108: 1651-1657 [Abstract]  
• Kirk, K E, Blackburn, E H (1995). An unusual sequence arrangement in the telomeres of the germ-line micronucleus in Tetrahymena thermophila.. Genes Dev. 9: 59-71 [Abstract]  
• Moretti, P, Freeman, K, Coodly, L, Shore, D (1994). Evidence that a complex of SIR proteins interacts with the silencer and telomere-binding protein RAP1.. Genes Dev. 8: 2257-2269 [Abstract]  
• Dobson, M., Pearlman, R., Karaiskakis, A, Spyropoulos, B, Moens, P. (1994). Synaptonemal complex proteins: occurrence, epitope mapping and chromosome disjunction. J. Cell Sci. 107: 2749-2760 [Abstract]  
• Morse-Gaudio, M, Risley, M. (1994). Topoisomerase II expression and VM-26 induction of DNA breaks during spermatogenesis in Xenopus laevis. J. Cell Sci. 107: 2887-2898 [Abstract]  
• Kyrion, G, Liu, K, Liu, C, Lustig, A J (1993). RAP1 and telomere structure regulate telomere position effects in Saccharomyces cerevisiae.. Genes Dev. 7: 1146-1159 [Abstract]  
• Cardenas, M., Gasser, S. (1993). Regulation of topoisomerase II by phosphorylation: a role for casein kinase II. J. Cell Sci. 104: 219-225  
• Thompson, J.S., Hecht, A., Grunstein, M. (1993). Histones and the Regulation of Heterochromatin in Yeast. Cold Spring Harb Symp Quant Biol 58: 247-256 [Abstract]  
• Palladino, F., Laroche, T., Gilson, E., Pillus, L., Gasser, S.M. (1993). The Positioning of Yeast Telomeres Depends on SIR3, SIR4, and the Integrity of the Nuclear Membrane. Cold Spring Harb Symp Quant Biol 58: 733-746 [Abstract]  
• Jones, M. H., He, X., Giddings, T. H., Winey, M. (2001). Yeast Dam1p has a role at the kinetochore in assembly of the mitotic spindle. Proc. Natl. Acad. Sci. USA 98: 13675-13680 [Abstract] [Full Text]  

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