100-kD proteins of Golgi- and trans-Golgi network-associated coated vesicles have related but distinct membrane binding properties

doi:10.1083/jcb.117.6.1171

This Article

	Full Text (PDF, 2730K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Wong, D. H.
	Articles by Brodsky, F. M.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Wong, D. H.
	Articles by Brodsky, F. M.


Social Bookmarking

	What's this?

ARTICLES

100-kD proteins of Golgi- and trans-Golgi network-associated coated vesicles have related but distinct membrane binding properties

DH Wong and FM Brodsky
Department of Pharmacy, School of Pharmacy, University of California San Francisco 94143-0446.

The 100-110-kD proteins (alpha-, beta-, beta'-, and gamma-adaptins) of clathrin-coated vesicles and the 110-kD protein (beta-COP) of the nonclathrin-coated vesicles that mediate constitutive transport through the Golgi have homologous protein sequences. To determine whether homologous processes are involved in assembly of the two types of coated vesicles, the membrane binding properties of their coat proteins were compared. After treatment of MDBK cells with the fungal metabolite Brefeldin A (BFA), beta-COP was redistributed to the cytoplasm within 15 s, gamma-adaptin and clathrin in the trans-Golgi network (TGN) dispersed within 30 s, but the alpha-adaptin and clathrin present on coated pits and vesicles derived from the plasma membrane remained membrane associated even after a 15-min exposure to BFA. In PtK1 cells and MDCK cells, BFA did not affect beta-COP binding or Golgi morphology but still induced redistribution of gamma-adaptin and clathrin from TGN membranes to the cytoplasm. Thus BFA affects the binding of coat proteins to membranes in the Golgi region (Golgi apparatus and TGN) but not plasma membranes. However, the Golgi binding interactions of beta- COP and gamma-adaptin are distinct and differentially sensitive to BFA. BFA treatment did not release gamma-adaptin or clathrin from purified clathrin-coated vesicles, suggesting that their distribution to the cytoplasm after BFA treatment of cells was due to interference with their rebinding to TGN membranes after a normal cycle of disassembly. This was confirmed using an in vitro assay in which gamma-adaptin binding to TGN membranes was blocked by BFA and enhanced by GTP gamma S, similar to the binding of beta-COP to Golgi membranes. These results suggest the involvement of GTP-dependent proteins in the association of the 100-kD coat proteins with membranes in the Golgi region of the cell.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Lanke, K. H. W., van der Schaar, H. M., Belov, G. A., Feng, Q., Duijsings, D., Jackson, C. L., Ehrenfeld, E., van Kuppeveld, F. J. M. (2009). GBF1, a Guanine Nucleotide Exchange Factor for Arf, Is Crucial for Coxsackievirus B3 RNA Replication. J. Virol. 83: 11940-11949 [Abstract] [Full Text]
Kametaka, S., Moriyama, K., Burgos, P. V., Eisenberg, E., Greene, L. E., Mattera, R., Bonifacino, J. S. (2007). Canonical Interaction of Cyclin G associated Kinase with Adaptor Protein 1 Regulates Lysosomal Enzyme Sorting. Mol. Biol. Cell 18: 2991-3001 [Abstract] [Full Text]
Gough, L. L., Fan, J., Chu, S., Winnick, S., Beck, K. A. (2003). Golgi Localization of Syne-1. Mol. Biol. Cell 14: 2410-2424 [Abstract] [Full Text]
Lefkir, Y., de Chassey, B., Dubois, A., Bogdanovic, A., Brady, R. J., Destaing, O., Bruckert, F., O'Halloran, T. J., Cosson, P., Letourneur, F. (2003). The AP-1 Clathrin-adaptor Is Required for Lysosomal Enzymes Sorting and Biogenesis of the Contractile Vacuole Complex in Dictyostelium Cells. Mol. Biol. Cell 14: 1835-1851 [Abstract] [Full Text]
Waguri, S., Dewitte, F., Le Borgne, R., Rouille, Y., Uchiyama, Y., Dubremetz, J.-F., Hoflack, B. (2003). Visualization of TGN to Endosome Trafficking through Fluorescently Labeled MPR and AP-1 in Living Cells. Mol. Biol. Cell 14: 142-155 [Abstract] [Full Text]
Gazina, E. V., Mackenzie, J. M., Gorrell, R. J., Anderson, D. A. (2002). Differential Requirements for COPI Coats in Formation of Replication Complexes among Three Genera of Picornaviridae. J. Virol. 76: 11113-11122 [Abstract] [Full Text]
Salamone, M. d. C., Mendiguren, A. K., Salamone, G. V., Fainboim, L. (2001). Membrane trafficking of CD1c on activated T cells. J. Leukoc. Biol. 70: 567-577 [Abstract] [Full Text]
Okamoto, C. T., McKinney, J., Jeng, Y. Y. (2000). Clathrin in mitotic spindles. Am. J. Physiol. Cell Physiol. 279: C369-C374 [Abstract] [Full Text]
Arneson, L. S., Kunz, J., Anderson, R. A., Traub, L. M. (1999). Coupled Inositide Phosphorylation and Phospholipase D Activation Initiates Clathrin-coat Assembly on Lysosomes. J. Biol. Chem. 274: 17794-17805 [Abstract] [Full Text]
Zhu, Y., Traub, L. M., Kornfeld, S. (1999). High-Affinity Binding Of The AP-1 Adaptor Complex to Trans-Golgi Network Membranes Devoid Of Mannose 6-Phosphate Receptors. Mol. Biol. Cell 10: 537-549 [Abstract] [Full Text]
Orzech, E., Schlessinger, K., Weiss, A., Okamoto, C. T., Aroeti, B. (1999). Interactions of the AP-1 Golgi Adaptor with the Polymeric Immunoglobulin Receptor and Their Possible Role in Mediating Brefeldin A-sensitive Basolateral Targeting from the trans-Golgi Network. J. Biol. Chem. 274: 2201-2215 [Abstract] [Full Text]
Chen, C.-Y., Graham, T. R. (1998). An arf1{Delta} Synthetic Lethal Screen Identifies a New Clathrin Heavy Chain Conditional Allele That Perturbs Vacuolar Protein Transport in Saccharomyces cerevisiae. Genetics 150: 577-589 [Abstract] [Full Text]
Ooi, C. E., Dell'Angelica, E. C., Bonifacino, J. S. (1998). ADP-Ribosylation Factor 1 (ARF1) Regulates Recruitment of the AP-3 Adaptor Complex to Membranes. JCB 142: 391-402 [Abstract] [Full Text]
Zhu, Y., Traub, L. M., Kornfeld, S. (1998). ADP-Ribosylation Factor 1�Transiently Activates High-Affinity Adaptor Protein Complex AP-1 Binding Sites On Golgi Membranes. Mol. Biol. Cell 9: 1323-1337 [Abstract] [Full Text]
Futter, C.E., Gibson, A., Allchin, E.H., Maxwell, S., Ruddock, L.J., Odorizzi, G., Domingo, D., Trowbridge, I.S., Hopkins, C.R. (1998). In Polarized MDCK Cells Basolateral Vesicles Arise from Clathrin-{gamma}-adaptin-coated Domains on Endosomal Tubules. JCB 141: 611-623 [Abstract] [Full Text]
Okamoto, C. T., Karam, S. M., Jeng, Y. Y., Forte, J. G., Goldenring, J. R. (1998). Identification of clathrin and clathrin adaptors on tubulovesicles of gastric acid secretory (oxyntic) cells. Am. J. Physiol. Cell Physiol. 274: C1017-C1029 [Abstract] [Full Text]
Drecktrah, D, de Figueiredo, P, Mason, R., Brown, W. (1998). Retrograde trafficking of both Golgi complex and TGN markers to the ER induced by nordihydroguaiaretic acid and cyclofenil diphenol. J. Cell Sci. 111: 951-965 [Abstract]
Liang, J. O., Kornfeld, S. (1997). Comparative Activity of ADP-ribosylation Factor Family Members in the Early Steps of Coated Vesicle Formation on Rat Liver Golgi Membranes. J. Biol. Chem. 272: 4141-4148 [Abstract] [Full Text]
Beck, K., Buchanan, J., Nelson, W. (1997). Golgi membrane skeleton: identification, localization and oligomerization of a 195 kDa ankyrin isoform associated with the Golgi complex. J. Cell Sci. 110: 1239-1249 [Abstract]
Seaman, M. N.J., Sowerby, P. J., Robinson, M. S. (1996). Cytosolic and Membrane-associated Proteins Involved in the Recruitment of AP-1 Adaptors onto the Trans-Golgi Network. J. Biol. Chem. 271: 25446-25451 [Abstract] [Full Text]
Tolbert, L. M., Lameh, J. (1996). Human Muscarinic Cholinergic Receptor Hm1 Internalizes via Clathrin-coated Vesicles. J. Biol. Chem. 271: 17335-17342 [Abstract] [Full Text]
Le Borgne, R., Griffiths, G., Hoflack, B. (1996). Mannose 6-Phosphate Receptors and ADP-ribosylation Factors Cooperate for High Affinity Interaction of the AP-1 Golgi Assembly Proteins with Membranes. J. Biol. Chem. 271: 2162-2170 [Abstract] [Full Text]
Mallet, W., Brodsky, F. (1996). A membrane-associated protein complex with selective binding to the clathrin coat adaptor AP1. J. Cell Sci. 109: 3059-3068 [Abstract]
Traub, L. M., Kornfeld, S., Ungewickell, E. (1995). Different Domains of the AP-1 Adaptor Complex Are Required for Golgi Membrane Binding and Clathrin Recruitment. J. Biol. Chem. 270: 4933-4942 [Abstract] [Full Text]
Simon, J.-P., Ivanov, I.E., Ren, M., Zeng, J., Shopsin, B., Hersh, D., Tempst, P., Erdjument-Bromage, H., Lui, M., De Lemos-Chiarandini, C., Rosenfeld, M., Gravotta, D., Morimoto, T., Adesnik, M., Sabatini, D.D. (1995). Regulation of Post-Golgi Vesicle Production in an In Vitro System. Cold Spring Harb Symp Quant Biol 60: 179-195 [Abstract]
Wagner, M, Rajasekaran, A., Hanzel, D., Mayor, S, Rodriguez-Boulan, E (1994). Brefeldin A causes structural and functional alterations of the trans-Golgi network of MDCK cells. J. Cell Sci. 107: 933-943 [Abstract]
Yan, R., Han, P., Miao, H., Greengard, P., Xu, H. (2001). The Transmembrane Domain of the Alzheimer's beta -Secretase (BACE1) Determines Its Late Golgi Localization and Access to beta -Amyloid Precursor Protein (APP) Substrate. J. Biol. Chem. 276: 36788-36796 [Abstract] [Full Text]