Nerve growth factor transcriptional control of c-fos promoter transfected in cultured spinal sensory neurons

doi:10.1083/jcb.118.1.131

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Nerve growth factor transcriptional control of c-fos promoter transfected in cultured spinal sensory neurons

N Gabellini, MC Minozzi, A Leon and R Dal Toso
Fidia Research Laboratories, Abano Terme, Italy.

High efficiency gene transfer (greater than 90%) in chicken dorsal root ganglion neurons has been obtained by DNA calcium phosphate co- precipitation, hence providing an important tool to study control of gene expression in primary neurons. Transfection with c-fos promoter sequences linked to the chloramphenicol acetyltransferase reporter gene showed that the serum responsive element functions as a strong transcriptional enhancer. Transcription from this element is developmentally regulated, and mediates the genetic response to nerve growth factor (NGF) in developing avian sensory neurons. Furthermore, NGF exerts a negative effect on transcription from the cyclic AMP responsive element, thereby supporting the involvement of tyrosine kinase activation by NGF in primary sensory neurons.
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