Kinesin-related proteins required for assembly of the mitotic spindle

doi:10.1083/jcb.118.1.95

This Article

	Full Text (PDF, 3418K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Roof, D. M.
	Articles by Rose, M. D.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Roof, D. M.
	Articles by Rose, M. D.


Social Bookmarking

	What's this?

ARTICLES

Kinesin-related proteins required for assembly of the mitotic spindle

DM Roof, PB Meluh and MD Rose
Department of Molecular Biology, Princeton University, New Jersey 08544.

We identified two new Saccharomyces cerevisiae kinesin-related genes, KIP1 and KIP2, using polymerase chain reaction primers corresponding to highly conserved regions of the kinesin motor domain. Both KIP proteins are expressed in vivo, but deletion mutations conferred no phenotype. Moreover, kip1 kip2 double mutants and a triple mutant with kinesin- related kar3 had no synthetic phenotype. Using a genetic screen for mutations that make KIP1 essential, we identified another gene, KSL2, which proved to be another kinesin-related gene, CIN8. KIP1 and CIN8 are functionally redundant: double mutants arrested in mitosis whereas the single mutants did not. The microtubule organizing centers of arrested cells were duplicated but unseparated, indicating that KIP1 or CIN8 is required for mitotic spindle assembly. Consistent with this role, KIP1 protein was found to colocalize with the mitotic spindle.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Chiroli, E., Rancati, G., Catusi, I., Lucchini, G., Piatti, S. (2009). Cdc14 Inhibition by the Spindle Assembly Checkpoint Prevents Unscheduled Centrosome Separation in Budding Yeast. Mol. Biol. Cell 20: 2626-2637 [Abstract] [Full Text]
Cui, H., Ghosh, S. K., Jayaram, M. (2009). The selfish yeast plasmid uses the nuclear motor Kip1p but not Cin8p for its localization and equal segregation. JCB 185: 251-264 [Abstract] [Full Text]
Movshovich, N., Fridman, V., Gerson-Gurwitz, A., Shumacher, I., Gertsberg, I., Fich, A., Hoyt, M. A., Katz, B., Gheber, L. (2008). Slk19-dependent mid-anaphase pause in kinesin-5-mutated cells. J. Cell Sci. 121: 2529-2539 [Abstract] [Full Text]
Simmons Kovacs, L. A., Nelson, C. L., Haase, S. B. (2008). Intrinsic and Cyclin-dependent Kinase-dependent Control of Spindle Pole Body Duplication in Budding Yeast. Mol. Biol. Cell 19: 3243-3253 [Abstract] [Full Text]
Amaro, I. A., Costanzo, M., Boone, C., Huffaker, T. C. (2008). The Saccharomyces cerevisiae Homolog of p24 Is Essential for Maintaining the Association of p150Glued With the Dynactin Complex. Genetics 178: 703-709 [Abstract] [Full Text]
Castillo, A., Morse, H. C. III, Godfrey, V. L., Naeem, R., Justice, M. J. (2007). Overexpression of Eg5 Causes Genomic Instability and Tumor Formation in Mice. Cancer Res. 67: 10138-10147 [Abstract] [Full Text]
Ma, L., McQueen, J., Cuschieri, L., Vogel, J., Measday, V. (2007). Spc24 and Stu2 Promote Spindle Integrity When DNA Replication Is Stalled. Mol. Biol. Cell 18: 2805-2816 [Abstract] [Full Text]
Hildebrandt, E. R., Gheber, L., Kingsbury, T., Hoyt, M. A. (2006). Homotetrameric Form of Cin8p, a Saccharomyces cerevisiae Kinesin-5 Motor, Is Essential for Its in Vivo Function. J. Biol. Chem. 281: 26004-26013 [Abstract] [Full Text]
Liu, M., Aneja, R., Liu, C., Sun, L., Gao, J., Wang, H., Dong, J.-T., Sarli, V., Giannis, A., Joshi, H. C., Zhou, J. (2006). Inhibition of the Mitotic Kinesin Eg5 Up-regulates Hsp70 through the Phosphatidylinositol 3-Kinase/Akt Pathway in Multiple Myeloma Cells. J. Biol. Chem. 281: 18090-18097 [Abstract] [Full Text]
Tytell, J. D., Sorger, P. K. (2006). Analysis of kinesin motor function at budding yeast kinetochores.. JCB 172: 861-874 [Abstract] [Full Text]
D'Silva, P. R., Schilke, B., Walter, W., Craig, E. A. (2005). Role of Pam16's degenerate J domain in protein import across the mitochondrial inner membrane. Proc. Natl. Acad. Sci. USA 102: 12419-12424 [Abstract] [Full Text]
Yoder, T. J., McElwain, M. A., Francis, S. E., Bagley, J., Muller, E. G.D., Pak, B., O'Toole, E. T., Winey, M., Davis, T. N. (2005). Analysis of a Spindle Pole Body Mutant Reveals a Defect in Biorientation and Illuminates Spindle Forces. Mol. Biol. Cell 16: 141-152 [Abstract] [Full Text]
Mackey, A. T., Sproul, L. R., Sontag, C. A., Satterwhite, L. L., Correia, J. J., Gilbert, S. P. (2004). Mechanistic Analysis of the Saccharomyces cerevisiae Kinesin Kar3. J. Biol. Chem. 279: 51354-51361 [Abstract] [Full Text]
Cochran, J. C., Sontag, C. A., Maliga, Z., Kapoor, T. M., Correia, J. J., Gilbert, S. P. (2004). Mechanistic Analysis of the Mitotic Kinesin Eg5. J. Biol. Chem. 279: 38861-38870 [Abstract] [Full Text]
Sakowicz, R., Finer, J. T., Beraud, C., Crompton, A., Lewis, E., Fritsch, A., Lee, Y., Mak, J., Moody, R., Turincio, R., Chabala, J. C., Gonzales, P., Roth, S., Weitman, S., Wood, K. W. (2004). Antitumor Activity of a Kinesin Inhibitor. Cancer Res. 64: 3276-3280 [Abstract] [Full Text]
D'Silva, P. D., Schilke, B., Walter, W., Andrew, A., Craig, E. A. (2003). J protein cochaperone of the mitochondrial inner membrane required for protein import into the mitochondrial matrix. Proc. Natl. Acad. Sci. USA 100: 13839-13844 [Abstract] [Full Text]
Grishanin, R. N., Klenchin, V. A., Loyet, K. M., Kowalchyk, J. A., Ann, K., Martin, T. F. J. (2002). Membrane Association Domains in Ca2+-dependent Activator Protein for Secretion Mediate Plasma Membrane and Dense-core Vesicle Binding Required for Ca2+-dependent Exocytosis. J. Biol. Chem. 277: 22025-22034 [Abstract] [Full Text]
Li, Y., Bachant, J., Alcasabas, A. A., Wang, Y., Qin, J., Elledge, S. J. (2002). The mitotic spindle is required for loading of the DASH complex onto the kinetochore. Genes Dev. 16: 183-197 [Abstract] [Full Text]
Hildebrandt, E. R., Hoyt, M. A. (2001). Cell Cycle-dependent Degradation of the Saccharomyces cerevisiae Spindle Motor Cin8p Requires APCCdh1 and a Bipartite Destruction Sequence. Mol. Biol. Cell 12: 3402-3416 [Abstract] [Full Text]
Prigozhina, N. L., Walker, R. A., Oakley, C. E., Oakley, B. R. (2001). gamma -Tubulin and the C-Terminal Motor Domain Kinesin-like Protein, KLPA, Function in the Establishment of Spindle Bipolarity in Aspergillus nidulans. Mol. Biol. Cell 12: 3161-3174 [Abstract] [Full Text]
Enquist-Newman, M., Cheeseman, I. M., Van Goor, D., Drubin, D. G., Meluh, P. B., Barnes, G. (2001). Dad1p, Third Component of the Duo1p/Dam1p Complex Involved in Kinetochore Function and Mitotic Spindle Integrity. Mol. Biol. Cell 12: 2601-2613 [Abstract] [Full Text]
Miki, H., Setou, M., Kaneshiro, K., Hirokawa, N. (2001). All kinesin superfamily protein, KIF, genes in mouse and human. Proc. Natl. Acad. Sci. USA 98: 7004-7011 [Abstract] [Full Text]
Cheeseman, I. M., Enquist-Newman, M., Muller-Reichert, T., Drubin, D. G., Barnes, G. (2001). Mitotic Spindle Integrity and Kinetochore Function Linked by the Duo1p/Dam1p Complex. JCB 152: 197-212 [Abstract] [Full Text]
Touitou, I, Lhomond, G, Pruliere, G (2001). Boursin, a sea urchin bimC kinesin protein, plays a role in anaphase and cytokinesis. J. Cell Sci. 114: 481-491 [Abstract]
Kapoor, T. M., Mayer, T. U., Coughlin, M. L., Mitchison, T. J. (2000). Probing Spindle Assembly Mechanisms with Monastrol, a Small Molecule Inhibitor of the Mitotic Kinesin, Eg5. JCB 150: 975-988 [Abstract] [Full Text]
Tabb, M. M., Tongaonkar, P., Vu, L., Nomura, M. (2000). Evidence for Separable Functions of Srp1p, the Yeast Homolog of Importin alpha (Karyopherin alpha ): Role for Srp1p and Sts1p in Protein Degradation. Mol. Cell. Biol. 20: 6062-6073 [Abstract] [Full Text]
Fujiwara, T., Tanaka, K., Inoue, E., Kikyo, M., Takai, Y. (1999). Bni1p Regulates Microtubule-Dependent Nuclear Migration through the Actin Cytoskeleton in Saccharomyces cerevisiae. Mol. Cell. Biol. 19: 8016-8027 [Abstract] [Full Text]
Cottingham, F. R., Gheber, L., Miller, D. L., Hoyt, M. A. (1999). Novel Roles for Saccharomyces cerevisiae Mitotic Spindle Motors. JCB 147: 335-350 [Abstract] [Full Text]
Gordon, D. M., Roof, D. M. (1999). The Kinesin-related Protein Kip1p of Saccharomyces cerevisiae Is Bipolar. J. Biol. Chem. 274: 28779-28786 [Abstract] [Full Text]
Dickson, K. S., Bilger, A., Ballantyne, S., Wickens, M. P. (1999). The Cleavage and Polyadenylation Specificity Factor in Xenopus laevis Oocytes Is a Cytoplasmic Factor Involved in Regulated Polyadenylation. Mol. Cell. Biol. 19: 5707-5717 [Abstract] [Full Text]
Jones, M. H., Bachant, J. B., Castillo, A. R., Giddings, T. H. Jr., Winey, M. (1999). Yeast Dam1p Is Required to Maintain Spindle Integrity during Mitosis and Interacts with the Mps1p Kinase. Mol. Biol. Cell 10: 2377-2391 [Abstract] [Full Text]
Kim, J.-h., Kang, J.-s., Chan, C. S.M. (1999). Sli15 Associates with the Ipl1 Protein Kinase to Promote Proper Chromosome Segregation in Saccharomyces cerevisiae. JCB 145: 1381-1394 [Abstract] [Full Text]
Hardwick, K. G., Li, R., Mistrot, C., Chen, R.-H., Dann, P., Rudner, A., Murray, A. W. (1999). Lesions in Many Different Spindle Components Activate the Spindle Checkpoint in the Budding Yeast Saccharomyces cerevisiae. Genetics 152: 509-518 [Abstract] [Full Text]
O'Toole, E. T., Winey, M., McIntosh, J. R. (1999). High-Voltage Electron Tomography of Spindle Pole Bodies and Early Mitotic Spindles in the Yeast Saccharomyces cerevisiae. Mol. Biol. Cell 10: 2017-2031 [Abstract] [Full Text]
Giet, R., Uzbekov, R., Cubizolles, F., Le Guellec, K., Prigent, C. (1999). The Xenopus laevis Aurora-related Protein Kinase pEg2 Associates with and Phosphorylates the Kinesin-related Protein XlEg5. J. Biol. Chem. 274: 15005-15013 [Abstract] [Full Text]
Gheber, L., Kuo, S. C., Hoyt, M. A. (1999). Motile Properties of the Kinesin-related Cin8p Spindle Motor Extracted from Saccharomyces cerevisiae Cells. J. Biol. Chem. 274: 9564-9572 [Abstract] [Full Text]
Manning, B. D., Barrett, J. G., Wallace, J. A., Granok, H., Snyder, M. (1999). Differential Regulation of the Kar3p Kinesin-related Protein by Two Associated Proteins, Cik1p and Vik1p. JCB 144: 1219-1233 [Abstract] [Full Text]
Biggins, S., Severin, F. F., Bhalla, N., Sassoon, I., Hyman, A. A., Murray, A. W. (1999). The conserved protein kinase Ipl1 regulates microtubule binding to kinetochores in budding yeast. Genes Dev. 13: 532-544 [Abstract] [Full Text]
Sharp, D. J., McDonald, K. L., Brown, H. M., Matthies, H. J., Walczak, C., Vale, R. D., Mitchison, T. J., Scholey, J. M. (1999). The Bipolar Kinesin, KLP61F, Cross-links Microtubules within Interpolar Microtubule Bundles of Drosophila Embryonic Mitotic Spindles. JCB 144: 125-138 [Abstract] [Full Text]
Hofmann, C., Cheeseman, I. M., Goode, B. L., McDonald, K. L., Barnes, G., Drubin, D. G. (1998). Saccharomyces cerevisiae Duo1p and Dam1p, Novel Proteins Involved in Mitotic Spindle Function. JCB 143: 1029-1040 [Abstract] [Full Text]
Straight, A. F., Sedat, J. W., Murray, A. W. (1998). Time-Lapse Microscopy Reveals Unique Roles for Kinesins during Anaphase in Budding Yeast. JCB 143: 687-694 [Abstract] [Full Text]
Segal, M., Clarke, D. J., Reed, S. I. (1998). Clb5-associated Kinase Activity is Required Early in the Spindle Pathway for Correct Preanaphase Nuclear Positioning in Saccharomyces cerevisiae. JCB 143: 135-145 [Abstract] [Full Text]
Ferhat, L., Cook, C., Chauviere, M., Harper, M., Kress, M., Lyons, G. E., Baas, P. W. (1998). Expression of the Mitotic Motor Protein Eg5 in Postmitotic Neurons: Implications for Neuronal Development. J. Neurosci. 18: 7822-7835 [Abstract] [Full Text]
Miller, R. K., Heller, K. K., Frisèn, L., Wallack, D. L., Loayza, D., Gammie, A. E., Rose, M. D. (1998). The Kinesin-related Proteins, Kip2p and Kip3p, Function Differently in Nuclear Migration in Yeast. Mol. Biol. Cell 9: 2051-2068 [Abstract] [Full Text]
Wigge, P. A., Jensen, O. N., Holmes, S., Soues, S., Mann, M., Kilmartin, J. V. (1998). Analysis of the Saccharomyces Spindle Pole by Matrix-assisted Laser Desorption/Ionization (MALDI) Mass Spectrometry. JCB 141: 967-977 [Abstract] [Full Text]
Miller, R. K., Rose, M. D. (1998). Kar9p Is a Novel Cortical Protein Required for Cytoplasmic Microtubule Orientation in Yeast. JCB 140: 377-390 [Abstract] [Full Text]
Drummond, D., Hagan, I. (1998). Mutations in the bimC box of Cut7 indicate divergence of regulation within the bimC family of kinesin related proteins. J. Cell Sci. 111: 853-865 [Abstract]
Huyett, A, Kahana, J, Silver, P, Zeng, X, Saunders, W. (1998). The Kar3p and Kip2p motors function antagonistically at the spindle poles to influence cytoplasmic microtubule numbers. J. Cell Sci. 111: 295-301 [Abstract]
Schwartz, K., Richards, K., Botstein, D. (1997). BIM1 Encodes a Microtubule-binding Protein in Yeast. Mol. Biol. Cell 8: 2677-2691 [Abstract] [Full Text]
Hoyt, M. A., Hyman, A. A., Bahler, M. (1997). Motor proteins of the eukaryotic�cytoskeleton. Proc. Natl. Acad. Sci. USA 94: 12747-12748 [Full Text]
Stearns, T. (1997). Motoring to the Finish: Kinesin and Dynein Work Together to Orient the Yeast Mitotic Spindle. JCB 138: 957-960 [Full Text]
DeZwaan, T. M., Ellingson, E., Pellman, D., Roof, D. M. (1997). Kinesin-related KIP3 of Saccharomyces cerevisiae Is Required for a Distinct Step in Nuclear Migration. JCB 138: 1023-1040 [Abstract] [Full Text]
Cottingham, F. R., Hoyt, M. A. (1997). Mitotic Spindle Positioning in Saccharomyces cerevisiae Is Accomplished by Antagonistically Acting Microtubule Motor Proteins. JCB 138: 1041-1053 [Abstract] [Full Text]
Nakagawa, T., Tanaka, Y., Matsuoka, E., Kondo, S., Okada, Y., Noda, Y., Kanai, Y., Hirokawa, N. (1997). Identification and classification of 16�new kinesin superfamily (KIF) proteins in mouse�genome. Proc. Natl. Acad. Sci. USA 94: 9654-9659 [Abstract] [Full Text]
Walczak, C. E., Verma, S., Mitchison, T. J. (1997). XCTK2: A Kinesin-related Protein That Promotes Mitotic Spindle Assembly in Xenopus laevis Egg Extracts. JCB 136: 859-870 [Abstract] [Full Text]
Cuevo, R. S., Garrett, S., Horowitz, J. M. (1997). Detection and Functional Characterization of p180, a Novel Cell Cycle Regulated Yeast Transcription Factor That Binds Retinoblastoma Control Elements. J. Biol. Chem. 272: 3813-3822 [Abstract] [Full Text]
Wilson, P., Fuller, M., Borisy, G. (1997). Monastral bipolar spindles: implications for dynamic centrosome organization. J. Cell Sci. 110: 451-464 [Abstract]
Asada, T, Kuriyama, R, Shibaoka, H (1997). TKRP125, a kinesin-related protein involved in the centrosome-independent organization of the cytokinetic apparatus in tobacco BY-2 cells. J. Cell Sci. 110: 179-189 [Abstract]
Lalo, D., Steffan, J. S., Dodd, J. A., Nomura, M. (1996). RRN11 Encodes the Third Subunit of the Complex Containing Rrn6p and Rrn7p That Is Essential for the Initiation of rDNA Transcription by Yeast RNA Polymerase I. J. Biol. Chem. 271: 21062-21067 [Abstract] [Full Text]
Keys, D A, Lee, B S, Dodd, J A, Nguyen, T T, Vu, L, Fantino, E, Burson, L M, Nogi, Y, Nomura, M (1996). Multiprotein transcription factor UAF interacts with the upstream element of the yeast RNA polymerase I promoter and forms a stable preinitiation complex.. Genes Dev. 10: 887-903 [Abstract]
Vashishtha, M., Walther, Z., Hall, J. L. (1996). The kinesin-homologous protein encoded by the Chlamydomonas FLA10 gene is associated with basal bodies and centrioles. J. Cell Sci. 109: 541-549 [Abstract]
Stirling, D., Rayner, T., Prescott, A., Stark, M. (1996). Mutations which block the binding of calmodulin to Spc110p cause multiple mitotic defects. J. Cell Sci. 109: 1297-1310 [Abstract]
Bauer, A, Kolling, R (1996). The SAC3 gene encodes a nuclear protein required for normal progression of mitosis. J. Cell Sci. 109: 1575-1583 [Abstract]
Masuda, H, Shibata, T (1996). Role of gamma-tubulin in mitosis-specific microtubule nucleation from the Schizosaccharomyces pombe spindle pole body. J. Cell Sci. 109: 165-177 [Abstract]
Goodson, H. V., Kang, S. J., Endow, S. A. (1994). Molecular phylogeny of the kinesin family of microtubule motor proteins. J. Cell Sci. 107: 1875-1884 [Abstract]
Kuriyama, R, Dragas-Granoic, S, Maekawa, T, Vassilev, A, Khodjakov, A, Kobayashi, H (1994). Heterogeneity and microtubule interaction of the CHO1 antigen, a mitosis-specific kinesin-like protein. Analysis of subdomains expressed in insect sf9 cells. J. Cell Sci. 107: 3485-3499 [Abstract]
Cary, R., Klymkowsky, M., Evans, R., Domingo, A, Dent, J., Backhus, L. (1994). Vimentin's tail interacts with actin-containing structures in vivo. J. Cell Sci. 107: 1609-1622 [Abstract]
Rodionov, V., Gelfand, V., Borisy, G. (1993). Kinesin-like molecules involved in spindle formation. J. Cell Sci. 106: 1179-1188 [Abstract]
Richardson, H, Lew, D J, Henze, M, Sugimoto, K, Reed, S I (1992). Cyclin-B homologs in Saccharomyces cerevisiae function in S phase and in G2.. Genes Dev. 6: 2021-2034 [Abstract]
Gordon, D. M., Roof, D. M. (2001). Degradation of the kinesin Kip1p at anaphase onset is mediated by the anaphase-promoting complex and Cdc20p. Proc. Natl. Acad. Sci. USA 98: 12515-12520 [Abstract] [Full Text]