Phosphorylation of myosin-II regulatory light chain by cyclin-p34cdc2: a mechanism for the timing of cytokinesis

doi:10.1083/jcb.118.3.595

This Article

Full Text (PDF, 2958K)

Alert me when this article is cited

Citation Map

Services

Email this article

Similar articles in this journal

Similar articles in PubMed

Alert me to new content in the JCB

Download to citation manager

Citing Articles

Citing Articles via HighWire

Citing Articles via CrossRef

Citing Articles via Google Scholar

Google Scholar

Articles by Satterwhite, L. L.

Articles by Pollard, T. D.

Search for Related Content

PubMed

PubMed Citation

Articles by Satterwhite, L. L.

Articles by Pollard, T. D.

Pubmed/NCBI databases

Substance via MeSH

Social Bookmarking

What's this?

ARTICLES

Phosphorylation of myosin-II regulatory light chain by cyclin-p34cdc2: a mechanism for the timing of cytokinesis

LL Satterwhite, MJ Lohka, KL Wilson, TY Scherson, LJ Cisek, JL Corden and TD Pollard
Department of Cell Biology and Anatomy, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.

To understand how cytokinesis is regulated during mitosis, we tested cyclin-p34cdc2 for myosin-II kinase activity, and investigated the mitotic-specific phosphorylation of myosin-II in lysates of Xenopus eggs. Purified cyclin-p34cdc2 phosphorylated the regulatory light chain of cytoplasmic and smooth muscle myosin-II in vitro on serine-1 or serine-2 and threonine-9, sites known to inhibit the actin-activated myosin ATPase activity of smooth muscle and nonmuscle myosin (Nishikawa, M., J. R. Sellers, R. S. Adelstein, and H. Hidaka. 1984. J. Biol. Chem. 259:8808-8814; Bengur, A. R., A. E. Robinson, E. Appella, and J. R. Sellers. 1987. J. Biol. Chem. 262:7613-7617; Ikebe, M., and S. Reardon. 1990. Biochemistry. 29:2713-2720). Serine-1 or -2 of the regulatory light chain of Xenopus cytoplasmic myosin-II was also phosphorylated in Xenopus egg lysates stabilized in metaphase, but not in interphase. Inhibition of myosin-II by cyclin-p34cdc2 during prophase and metaphase could delay cytokinesis until chromosome segregation is initiated and thus determine the timing of cytokinesis relative to earlier events in mitosis.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Kong, X., Gan, H., Hao, Y., Cheng, C., Jiang, J., Hong, Y., Yang, J., Zhu, H., Chi, Y., Yun, X., Gu, J. (2009). CDK11p58 Phosphorylation of PAK1 Ser174 Promotes DLC2 Binding and Roles on Cell Cycle Progression. J Biochem 146: 417-427 [Abstract] [Full Text]
Satoh, R., Morita, T., Takada, H., Kita, A., Ishiwata, S., Doi, A., Hagihara, K., Taga, A., Matsumura, Y., Tohda, H., Sugiura, R. (2009). Role of the RNA-binding Protein Nrd1 and Pmk1 Mitogen-activated Protein Kinase in the Regulation of Myosin mRNA Stability in Fission Yeast. Mol. Biol. Cell 20: 2473-2485 [Abstract] [Full Text]
Komatsu, S., Ikebe, M. (2007). The Phosphorylation of Myosin II at the Ser1 and Ser2 Is Critical for Normal Platelet-derived Growth Factor induced Reorganization of Myosin Filaments. Mol. Biol. Cell 18: 5081-5090 [Abstract] [Full Text]
Lucero, A., Stack, C., Bresnick, A. R., Shuster, C. B. (2006). A Global, Myosin Light Chain Kinase-dependent Increase in Myosin II Contractility Accompanies the Metaphase-Anaphase Transition in Sea Urchin Eggs. Mol. Biol. Cell 17: 4093-4104 [Abstract] [Full Text]
Lin, Y. M., Teng, Y. N., Chung, C. L., Tsai, W. C., Lin, Y. H., Lin, J. S. N., Kuo, P. L. (2006). Decreased mRNA transcripts of M-phase promoting factor and its regulators in the testes of infertile men. Hum Reprod 21: 138-144 [Abstract] [Full Text]
Brunet, S., Maro, B. (2005). Cytoskeleton and cell cycle control during meiotic maturation of the mouse oocyte: integrating time and space. Reproduction 130: 801-811 [Abstract] [Full Text]
Fazal, F., Gu, L., Ihnatovych, I., Han, Y., Hu, W., Antic, N., Carreira, F., Blomquist, J. F., Hope, T. J., Ucker, D. S., de Lanerolle, P. (2005). Inhibiting Myosin Light Chain Kinase Induces Apoptosis In Vitro and In Vivo. Mol. Cell. Biol. 25: 6259-6266 [Abstract] [Full Text]
Dulyaninova, N. G., Patskovsky, Y. V., Bresnick, A. R. (2004). The N-terminus of the long MLCK induces a disruption in normal spindle morphology and metaphase arrest. J. Cell Sci. 117: 1481-1493 [Abstract] [Full Text]
Maddox, A. S., Burridge, K. (2003). RhoA is required for cortical retraction and rigidity during mitotic cell rounding. JCB 160: 255-265 [Abstract] [Full Text]
Mulvihill, D. P., Hyams, J. S. (2002). Cytokinetic actomyosin ring formation and septation in fission yeast are dependent on the full recruitment of the polo-like kinase Plo1 to the spindle pole body and a functional spindle assembly checkpoint. J. Cell Sci. 115: 3575-3586 [Abstract] [Full Text]
Royou, A., Sullivan, W., Karess, R. (2002). Cortical recruitment of nonmuscle myosin II in early syncytial Drosophila embryos: its role in nuclear axial expansion and its regulation by Cdc2 activity. JCB 158: 127-137 [Abstract] [Full Text]
Guertin, D. A., Trautmann, S., McCollum, D. (2002). Cytokinesis in Eukaryotes. Microbiol. Mol. Biol. Rev. 66: 155-178 [Abstract] [Full Text]
Noguchi, T., Mabuchi, I. (2002). Localized Calcium Signals along the Cleavage Furrow of the Xenopus Egg Are Not Involved in Cytokinesis. Mol. Biol. Cell 13: 1263-1273 [Abstract] [Full Text]
Chew, T.-L., Wolf, W. A., Gallagher, P. J., Matsumura, F., Chisholm, R. L. (2002). A fluorescent resonant energy transfer-based biosensor reveals transient and regional myosin light chain kinase activation in lamella and cleavage furrows. JCB 156: 543-553 [Abstract] [Full Text]
Noguchi, T, Mabuchi, I (2001). Reorganization of actin cytoskeleton at the growing end of the cleavage furrow of Xenopus egg during cytokinesis. J. Cell Sci. 114: 401-412 [Abstract]
McGuire, S. L., Roe, D. L., Carter, B. W., Carter, R. L., Grace, S. P., Hays, P. L., Lang, G. A., Mamaril, J. L. C., McElvaine, A. T., Payne, A. M., Schrader, M. D., Wahrle, S. E., Young, C. D. (2000). Extragenic Suppressors of the nimX2cdc2 Mutation of Aspergillus nidulans Affect Nuclear Division, Septation and Conidiation. Genetics 156: 1573-1584 [Abstract] [Full Text]
Poperechnaya, A., Varlamova, O., Lin, P.-j., Stull, J. T., Bresnick, A. R. (2000). Localization and Activity of Myosin Light Chain Kinase Isoforms during the Cell Cycle. JCB 151: 697-708 [Abstract] [Full Text]
Wei, Q., Adelstein, R. S. (2000). Conditional Expression of a Truncated Fragment of Nonmuscle Myosin II-A Alters Cell Shape but Not Cytokinesis in HeLa Cells. Mol. Biol. Cell 11: 3617-3627 [Abstract] [Full Text]
Le Goff, X, Motegi, F, Salimova, E, Mabuchi, I, Simanis, V (2000). The S. pombe rlc1 gene encodes a putative myosin regulatory light chain that binds the type II myosins myo3p and myo2p. J. Cell Sci. 113: 4157-4163 [Abstract]
Balasubramanian, M., McCollum, D, Surana, U (2000). Tying the knot: linking cytokinesis to the nuclear cycle. J. Cell Sci. 113: 1503-1513 [Abstract]
Motegi, F, Nakano, K, Mabuchi, I (2000). Molecular mechanism of myosin-II assembly at the division site in Schizosaccharomyces pombe. J. Cell Sci. 113: 1813-1825 [Abstract]
Foe, V., Field, C., Odell, G. (2000). Microtubules and mitotic cycle phase modulate spatiotemporal distributions of F-actin and myosin II in Drosophila syncytial blastoderm embryos. Development 127: 1767-1787 [Abstract]
Zeng, Q, Lagunoff, D, Masaracchia, R, Goeckeler, Z, Cote, G, Wysolmerski, R (2000). Endothelial cell retraction is induced by PAK2 monophosphorylation of myosin II. J. Cell Sci. 113: 471-482 [Abstract]
Wolkow, T. D., Mirabito, P. M., Venkatram, S., Hamer, J. E. (2000). Hypomorphic bimAAPC3 Alleles Cause Errors in Chromosome Metabolism That Activate the DNA Damage Checkpoint Blocking Cytokinesis in Aspergillus nidulans. Genetics 154: 167-179 [Abstract] [Full Text]
Ikebe, R., Reardon, S., Mitsui, T., Ikebe, M. (1999). Role of the N-terminal Region of the Regulatory Light Chain in the Dephosphorylation of Myosin by Myosin Light Chain Phosphatase. J. Biol. Chem. 274: 30122-30126 [Abstract] [Full Text]
Shuster, C. B., Burgess, D. R. (1999). Parameters That Specify the Timing of Cytokinesis. JCB 146: 981-992 [Abstract] [Full Text]
Totsukawa, G., Yamakita, Y., Yamashiro, S., Hosoya, H., Hartshorne, D. J., Matsumura, F. (1999). Activation of Myosin Phosphatase Targeting Subunit by Mitosis-specific Phosphorylation. JCB 144: 735-744 [Abstract] [Full Text]
Shannon, K. B., Li, R. (1999). The Multiple Roles of Cyk1p in the Assembly and Function of the Actomyosin Ring in Budding Yeast. Mol. Biol. Cell 10: 283-296 [Abstract] [Full Text]
Schumacher, J. M., Golden, A., Donovan, P. J. (1998). AIR-2: An Aurora/Ipl1-related Protein Kinase Associated with Chromosomes and Midbody Microtubules Is Required for Polar Body Extrusion and Cytokinesis in Caenorhabditis elegans Embryos. JCB 143: 1635-1646 [Abstract] [Full Text]
Yasui, Y., Amano, M., Nagata, K.-i., Inagaki, N., Nakamura, H., Saya, H., Kaibuchi, K., Inagaki, M. (1998). Roles of Rho-associated Kinase in Cytokinesis; Mutations in Rho-associated Kinase Phosphorylation Sites Impair Cytokinetic Segregation of Glial Filaments. JCB 143: 1249-1258 [Abstract] [Full Text]
Cai, S., Pestic-Dragovich, L., O'Donnell, M. E., Wang, N., Ingber, D., Elson, E., De Lanerolle, P. (1998). Regulation of cytoskeletal mechanics and cell growth by myosin light chain phosphorylation. Am. J. Physiol. Cell Physiol. 275: C1349-C1356 [Abstract] [Full Text]
Simerly, C., Nowak, G., de Lanerolle, P., Schatten, G. (1998). Differential Expression and Functions of Cortical Myosin IIA and IIB Isotypes during Meiotic Maturation, Fertilization, and Mitosis in Mouse Oocytes and Embryos. Mol. Biol. Cell 9: 2509-2525 [Abstract] [Full Text]
Sakurada, K., Seto, M., Sasaki, Y. (1998). Dynamics of myosin light chain phosphorylation at Ser19 and Thr18/Ser19 in smooth muscle cells in culture. Am. J. Physiol. Cell Physiol. 274: C1563-C1572 [Abstract] [Full Text]
Hinchcliffe, E. H., Cassels, G. O., Rieder, C. L., Sluder, G. (1998). The Coordination of Centrosome Reproduction with Nuclear Events of the Cell Cycle in the Sea Urchin Zygote. JCB 140: 1417-1426 [Abstract] [Full Text]
Harris, S. D., Kraus, P. R. (1998). Regulation of Septum Formation in Aspergillus nidulans by a DNA Damage Checkpoint Pathway. Genetics 148: 1055-1067 [Abstract] [Full Text]
Grallert, A., Grallert, B., Ribar, B., Sipiczki, M. (1998). Coordination of Initiation of Nuclear Division and Initiation of Cell Division in Schizosaccharomyces pombe: Genetic Interactions of Mutations. J. Bacteriol. 180: 892-900 [Abstract] [Full Text]
Lippincott, J., Li, R. (1998). Sequential Assembly of Myosin II, an IQGAP-like Protein, and Filamentous Actin to a Ring Structure Involved in Budding Yeast Cytokinesis. JCB 140: 355-366 [Abstract] [Full Text]
Perez-Mongiovi, D, Chang, P, Houliston, E (1998). A propagated wave of MPF activation accompanies surface contraction waves at first mitosis in Xenopus. J. Cell Sci. 111: 385-393 [Abstract]
Sohrmann, M., Schmidt, S., Hagan, I., Simanis, V. (1998). Asymmetric segregation on spindle poles of the Schizosaccharomyces pombe septum-inducing protein kinase�Cdc7p. Genes Dev. 12: 84-94 [Abstract] [Full Text]
Zang, J.-H., Cavet, G., Sabry, J. H., Wagner, P., Moores, S. L., Spudich, J. A. (1997). On the Role of Myosin-II in Cytokinesis: Division of Dictyostelium Cells under Adhesive and Nonadhesive Conditions. Mol. Biol. Cell 8: 2617-2629 [Abstract] [Full Text]
Gould, K. L., Simanis, V. (1997). The control of septum formation in fission�yeast. Genes Dev. 11: 2939-2951 [Full Text]
Wheatley, S. P., Hinchcliffe, E. H., Glotzer, M., Hyman, A. A., Sluder, G., Wang, Y.-l. (1997). CDK1 Inactivation Regulates Anaphase Spindle Dynamics and Cytokinesis In Vivo. JCB 138: 385-393 [Abstract] [Full Text]
Adachi, H., Takahashi, Y., Hasebe, T., Shirouzu, M., Yokoyama, S., Sutoh, K. (1997). Dictyostelium IQGAP-related Protein Specifically Involved in the Completion of Cytokinesis. JCB 137: 891-898 [Abstract] [Full Text]
Cordenonsi, M, Mazzon, E, De Rigo, L, Baraldo, S, Meggio, F, Citi, S (1997). Occludin dephosphorylation in early development of Xenopus laevis. J. Cell Sci. 110: 3131-3139 [Abstract]
Walker, G., Shuster, C., Burgess, D. (1997). Microtubule-entrained kinase activities associated with the cortical cytoskeleton during cytokinesis. J. Cell Sci. 110: 1373-1386 [Abstract]
Holmes, JenniferK., Solomon, MarkJ. (1996). A Predictive Scale for Evaluating Cyclin-dependent Kinase Substrates. A COMPARISON OF p34cdc2 AND p33cdk2. J. Biol. Chem. 271: 25240-25246 [Abstract] [Full Text]
Pato, M. D., Sellers, J. R., Preston, Y. A., Harvey, E. V., Adelstein, R. S. (1996). Baculovirus Expression of Chicken Nonmuscle Heavy Meromyosin II-B. J. Biol. Chem. 271: 2689-2695 [Abstract] [Full Text]
Bosc, D. G., Slominski, E., Sichler, C., Litchfield, D. W. (1995). Phosphorylation of Casein Kinase II by p34[IMAGE]. J. Biol. Chem. 270: 25872-25878 [Abstract] [Full Text]
Kelley, C. A., Oberman, F., Yisraeli, J. K., Adelstein, R. S. (1995). A Xenopus Nonmuscle Myosin Heavy Chain Isoform Is Phosphorylated by Cyclin-p34[IMAGE] Kinase during Meiosis. J. Biol. Chem. 270: 1395-1401 [Abstract] [Full Text]
Mabuchi, I. (1994). Cleavage furrow: timing of emergence of contractile ring actin filaments and establishment of the contractile ring by filament bundling in sea urchin eggs. J. Cell Sci. 107: 1853-1862 [Abstract]
Jimenez, M, Goday, C (1993). A centrosome-associated antibody from Drosophila melanogaster reveals a new microtubule-dependent structure in the equatorial zone of Parascaris univalens embryos. J. Cell Sci. 106: 719-730 [Abstract]
Chew, T.-L., Wolf, W. A., Gallagher, P. J., Matsumura, F., Chisholm, R. L. (2002). A fluorescent resonant energy transfer-based biosensor reveals transient and regional myosin light chain kinase activation in lamella and cleavage furrows. JCB 156: 543-553 [Abstract] [Full Text]