The Journal of Cell Biology, Vol 118, 1245-1258, Copyright © 1992 by The Rockefeller University Press

ARTICLES

Nectadrin, the heat-stable antigen, is a cell adhesion molecule

G Kadmon, M Eckert, M Sammar, M Schachner and P Altevogt
Department of Immunology and Genetics, German Centre for Cancer Research, Heidelberg.

Nectadrin, the cell surface glycoprotein recognized by the novel mAb 79, was found to be immunologically identical to the heat-stable antigen (HSA). It is a glycoprotein with a polypeptide core of only 30 amino acids and a very high carbohydrate content (Wenger, R. H., M. Ayane, R. Bose, G. Kohler, and P. J. Nielsen. 1991. Eur. J. Immunol. 21:1039-1046). Immunocytological studies using cultured splenic B- lymphocytes, neuroblastoma cells, and cerebellar cells indicated that nectadrin is preferentially expressed at sites of cell-cell contact. Purified nectadrin and monoclonal nectadrin antibody 79, but not other monoclonal nectadrin antibodies, inhibited the aggregation of B- lymphocytes by 70%, suggesting that nectadrin may act as a cell adhesion molecule. Nectadrin was purified from a mouse lymphoma cell line in two forms of 40-60 and 23-30 kD. The lower molecular weight form appears to be generated from the higher molecular weight form by degradative removal of saccharide residues characteristic of complex type oligosaccharide side chains. Latex beads coated with purified nectadrin aggregated and the rate of their aggregation depended on the molecular form of nectadrin, with the larger form being more potent than the smaller one in mediating bead aggregation. Nectadrin thus appears to be a self-binding cell adhesion molecule of a structurally novel type in that its extensive glycan structures may be implicated in mediating cell adhesion.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Facebook    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

• Lieberoth, A., Splittstoesser, F., Katagihallimath, N., Jakovcevski, I., Loers, G., Ranscht, B., Karagogeos, D., Schachner, M., Kleene, R. (2009). Lewisx and {alpha}2,3-Sialyl Glycans and Their Receptors TAG-1, Contactin, and L1 Mediate CD24-Dependent Neurite Outgrowth. J. Neurosci. 29: 6677-6690 [Abstract] [Full Text]  
• Smith, S. C., Oxford, G., Wu, Z., Nitz, M. D., Conaway, M., Frierson, H. F., Hampton, G., Theodorescu, D. (2006). The Metastasis-Associated Gene CD24 Is Regulated by Ral GTPase and Is a Mediator of Cell Proliferation and Survival in Human Cancer. Cancer Res. 66: 1917-1922 [Abstract] [Full Text]  
• Baumann, P., Cremers, N., Kroese, F., Orend, G., Chiquet-Ehrismann, R., Uede, T., Yagita, H., Sleeman, J. P. (2005). CD24 Expression Causes the Acquisition of Multiple Cellular Properties Associated with Tumor Growth and Metastasis. Cancer Res. 65: 10783-10793 [Abstract] [Full Text]  
• Fiume, R., Faenza, I., Matteucci, A., Astolfi, A., Vitale, M., Martelli, A. M., Cocco, L. (2005). Nuclear Phospholipase C {beta}1 (PLC{beta}1) Affects CD24 Expression in Murine Erythroleukemia Cells. J. Biol. Chem. 280: 24221-24226 [Abstract] [Full Text]  
• Jung, K. C., Park, W. S., Kim, H. J., Choi, E. Y., Kook, M.-C., Lee, H.-W., Bae, Y. (2004). TCR-Independent and Caspase-Independent Apoptosis of Murine Thymocytes by CD24 Cross-Linking. J. Immunol. 172: 795-802 [Abstract] [Full Text]  
• Re, F., Belyanskaya, S. L., Riese, R. J., Cipriani, B., Fischer, F. R., Granucci, F., Ricciardi-Castagnoli, P., Brosnan, C., Stern, L. J., Strominger, J. L., Santambrogio, L. (2002). Granulocyte-Macrophage Colony-Stimulating Factor Induces an Expression Program in Neonatal Microglia That Primes Them for Antigen Presentation. J. Immunol. 169: 2264-2273 [Abstract] [Full Text]  
• Belvindrah, R., Rougon, G., Chazal, G. (2002). Increased Neurogenesis in Adult mCD24-Deficient Mice. J. Neurosci. 22: 3594-3607 [Abstract] [Full Text]  
• Qin, G., Takenaka, T., Telsch, K., Kelley, L., Howard, T., Levade, T., Deans, R., Howard, B. H., Malech, H. L., Brady, R. O., Medin, J. A. (2001). Preselective gene therapy for Fabry disease. Proc. Natl. Acad. Sci. USA 10.1073/pnas.061020598v1 [Abstract] [Full Text]  
• Zhou, Q., Guo, Y., Liu, Y. (1998). Regulation of the Stability of Heat-Stable Antigen mRNA by Interplay between Two Novel cis Elements in the 3' Untranslated Region. Mol. Cell. Biol. 18: 815-826 [Abstract] [Full Text]  
• Aigner, S., Sthoeger, Z. M., Fogel, M., Weber, E., Zarn, J., Ruppert, M., Zeller, Y., Vestweber, D., Stahel, R., Sammar, M., Altevogt, P. (1997). CD24, a Mucin-Type Glycoprotein, Is a Ligand for P-Selectin on Human Tumor Cells. Blood 89: 3385-3395 [Abstract] [Full Text]  
• Nielsen, P.J., Lorenz, B., Muller, A.M., Wenger, R.H., Brombacher, F., Simon, M., von der Weid, T., Langhorne, J., Mossmann, H., Kohler, G. (1997). Altered Erythrocytes and a Leaky Block in B-Cell Development in CD24/HSA-Deficient Mice. Blood 89: 1058-1067 [Abstract] [Full Text]  
• Magnaldo, T, Barrandon, Y (1996). CD24 (heat stable antigen, nectadrin), a novel keratinocyte differentiation marker, is preferentially expressed in areas of the hair follicle containing the colony-forming cells. J. Cell Sci. 109: 3035-3045 [Abstract]  
• Kilger, G., Needham, L. A., Nielsen, P. J., Clements, J., Vestweber, D., Holzmann, B. (1995). Differential Regulation of alpha(4) Integrin-dependent Binding to Domains 1 and 4 of Vascular Cell Adhesion Molecule-1. J. Biol. Chem. 270: 5979-5984 [Abstract] [Full Text]  
• Qin, G., Takenaka, T., Telsch, K., Kelley, L., Howard, T., Levade, T., Deans, R., Howard, B. H., Malech, H. L., Brady, R. O., Medin, J. A. (2001). Preselective gene therapy for Fabry disease. Proc. Natl. Acad. Sci. USA 98: 3428-3433 [Abstract] [Full Text]  

Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents