Clathrin heavy chain is required for pinocytosis, the presence of large vacuoles, and development in Dictyostelium

doi:10.1083/jcb.118.6.1371

This Article

	Full Text (PDF, 2131K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by O'Halloran, T. J.
	Articles by Anderson, R. G.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by O'Halloran, T. J.
	Articles by Anderson, R. G.


Social Bookmarking

	What's this?

ARTICLES

Clathrin heavy chain is required for pinocytosis, the presence of large vacuoles, and development in Dictyostelium

TJ O'Halloran and RG Anderson
Department of Cell Biology and Neuroscience, University of Texas Southwestern Medical Center, Dallas 75235.

To investigate the intracellular role of the clathrin heavy chain in living cells, we have used "antisense" RNA to engineer mutant Dictyostelium discoideum cells that are severely deficient in clathrin heavy chain expression. Immunoblots stained with an anti-clathrin heavy chain antiserum revealed that mutant cells contained undetectable amounts of clathrin heavy chain protein. Similarly, Northern blots showed an absence of clathrin heavy chain mRNA. Clathrin heavy chain- deficient Dictyostelium cells were viable, but exhibited growth rates twofold slower than parental cells. Whereas many morphological features of the mutant cells were normal, mutant cells lacked coated pits and coated vesicles. Clathrin-deficient cells were also missing large translucent vacuoles that serve as endosomes and contractile vacuoles. In the absence of clathrin heavy chain, mutant cells displayed three distinct functional defects: (a) impairment in endocytosis of fluid phase markers, but competence in another endocytic pathway, the phagocytosis of solid particles; (b) defects in osmoregulation; and (c) inability to complete the starvation-induced development cycle.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Wen, Y., Stavrou, I., Bersuker, K., Brady, R. J., De Lozanne, A., O'Halloran, T. J. (2009). AP180-Mediated Trafficking of Vamp7B Limits Homotypic Fusion of Dictyostelium Contractile Vacuoles. Mol. Biol. Cell 20: 4278-4288 [Abstract] [Full Text]
Kriebel, P. W., Barr, V. A., Rericha, E. C., Zhang, G., Parent, C. A. (2008). Collective cell migration requires vesicular trafficking for chemoattractant delivery at the trailing edge. JCB 183: 949-961 [Abstract] [Full Text]
Brady, R. J., Wen, Y., O'Halloran, T. J. (2008). The ENTH and C-terminal domains of Dictyostelium epsin cooperate to regulate the dynamic interaction with clathrin-coated pits. J. Cell Sci. 121: 3433-3444 [Abstract] [Full Text]
Calvo-Garrido, J., Carilla-Latorre, S., Lazaro-Dieguez, F., Egea, G., Escalante, R. (2008). Vacuole Membrane Protein 1 Is an Endoplasmic Reticulum Protein Required for Organelle Biogenesis, Protein Secretion, and Development. Mol. Biol. Cell 19: 3442-3453 [Abstract] [Full Text]
Damer, C. K., Bayeva, M., Kim, P. S., Ho, L. K., Eberhardt, E. S., Socec, C. I., Lee, J. S., Bruce, E. A., Goldman-Yassen, A. E., Naliboff, L. C. (2007). Copine A Is Required for Cytokinesis, Contractile Vacuole Function, and Development in Dictyostelium. Eukaryot Cell 6: 430-442 [Abstract] [Full Text]
Stavrou, I., O'Halloran, T. J. (2006). The Monomeric Clathrin Assembly Protein, AP180, Regulates Contractile Vacuole Size in Dictyostelium discoideum. Mol. Biol. Cell 17: 5381-5389 [Abstract] [Full Text]
Mercanti, V., Blanc, C., Lefkir, Y., Cosson, P., Letourneur, F. (2006). Acidic clusters target transmembrane proteins to the contractile vacuole in Dictyostelium cells. J. Cell Sci. 119: 837-845 [Abstract] [Full Text]
Sabe, H. (2003). Requirement for Arf6 in Cell Adhesion, Migration, and Cancer Cell Invasion. J Biochem 134: 485-489 [Abstract] [Full Text]
Lefkir, Y., de Chassey, B., Dubois, A., Bogdanovic, A., Brady, R. J., Destaing, O., Bruckert, F., O'Halloran, T. J., Cosson, P., Letourneur, F. (2003). The AP-1 Clathrin-adaptor Is Required for Lysosomal Enzymes Sorting and Biogenesis of the Contractile Vacuole Complex in Dictyostelium Cells. Mol. Biol. Cell 14: 1835-1851 [Abstract] [Full Text]
Janssen, K.-P., Rost, R., Eichinger, L., Schleicher, M. (2001). Characterization of CD36/LIMPII Homologues in Dictyostelium discoideum. J. Biol. Chem. 276: 38899-38910 [Abstract] [Full Text]
Temesvari, L., Zhang, L., Fodera, B., Janssen, K.-P., Schleicher, M., Cardelli, J. A. (2000). Inactivation of lmpA, Encoding a LIMPII-related Endosomal Protein, Suppresses the Internalization and Endosomal Trafficking Defects in Profilin-null Mutants. Mol. Biol. Cell 11: 2019-2031 [Abstract] [Full Text]
Damer, C. K., O'Halloran, T. J. (2000). Spatially Regulated Recruitment of Clathrin to the Plasma Membrane during Capping and Cell Translocation. Mol. Biol. Cell 11: 2151-2159 [Abstract] [Full Text]
Noegel, A., Schleicher, M (2000). The actin cytoskeleton of Dictyostelium: a story told by mutants. J. Cell Sci. 113: 759-766 [Abstract]
Aguado-Velasco, C., Bretscher, M. S. (1999). Circulation of the Plasma Membrane in Dictyostelium. Mol. Biol. Cell 10: 4419-4427 [Abstract] [Full Text]
Kwak, E., Gerald, N., Larochelle, D. A., Vithalani, K. K., Niswonger, M. L., Maready, M., De Lozanne, A. (1999). LvsA, a Protein Related to the Mouse Beige Protein, Is Required for Cytokinesis in Dictyostelium. Mol. Biol. Cell 10: 4429-4439 [Abstract] [Full Text]
DeFIFE, K. M., JENNEY, C. R., COLTON, E., ANDERSON, J. M. (1999). Disruption of filamentous actin inhibits human macrophage fusion. FASEB J. 13: 823-832 [Abstract] [Full Text]
Seastone, D. J., Zhang, L., Buczynski, G., Rebstein, P., Weeks, G., Spiegelman, G., Cardelli, J. (1999). The Small Mr Ras-like GTPase Rap1 and the Phospholipase C Pathway Act to Regulate Phagocytosis in Dictyostelium discoideum. Mol. Biol. Cell 10: 393-406 [Abstract] [Full Text]
Journet, A, Chapel, A, Jehan, S, Adessi, C, Freeze, H, Klein, G, Garin, J (1999). Characterization of Dictyostelium discoideum cathepsin D. J. Cell Sci. 112: 3833-3843 [Abstract]
Perry, D. G., Daugherty, G. L., Martin, W. J. II (1999). Clathrin-Coated Pit-Associated Proteins Are Required for Alveolar Macrophage Phagocytosis. J. Immunol. 162: 380-386 [Abstract] [Full Text]
Laurent, O., Bruckert, F., Adessi, C., Satre, M. (1998). In Vitro Reconstituted Dictyostelium discoideum Early Endosome Fusion Is Regulated by Rab7 but Proceeds in the Absence of ATP-Mg2+ from the Bulk Solution. J. Biol. Chem. 273: 793-799 [Abstract] [Full Text]
Niswonger, M. L., O'Halloran, T. J. (1997). A novel role for clathrin in�cytokinesis. Proc. Natl. Acad. Sci. USA 94: 8575-8578 [Abstract] [Full Text]
Buczynski, G., Grove, B., Nomura, A., Kleve, M., Bush, J., Firtel, R. A., Cardelli, J. (1997). Inactivation of Two Dictyostelium discoideum Genes, DdPIK1 and DdPIK2, Encoding Proteins Related to Mammalian Phosphatidylinositide 3-kinases, Results in Defects in Endocytosis, Lysosome to Postlysosome Transport, and Actin Cytoskeleton Organization. JCB 136: 1271-1286 [Abstract] [Full Text]
Kokoza, V. A., Raikhel, A. S. (1997). Ovarian- and Somatic-specific Transcripts of the Mosquito Clathrin Heavy Chain Gene Generated by Alternative 5'-Exon Splicing and Polyadenylation. J. Biol. Chem. 272: 1164-1170 [Abstract] [Full Text]
Hacker, U, Albrecht, R, Maniak, M (1997). Fluid-phase uptake by macropinocytosis in Dictyostelium. J. Cell Sci. 110: 105-112 [Abstract]
Niswonger, M., O'Halloran, T. (1997). Clathrin heavy chain is required for spore cell but not stalk cell differentiation in Dictyostelium discoideum. Development 124: 443-451 [Abstract]
Temesvari, L. A., Bush, J. M., Peterson, M. D., Novak, K. D., Titus, M. A., Cardelli, J. A. (1996). Examination of the endosomal and lysosomal pathways in Dictyostelium discoideum myosin I mutants. J. Cell Sci. 109: 663-673 [Abstract]
Temesvari, L., Rodriguez-Paris, J., Bush, J., Zhang, L, Cardelli, J. (1996). Involvement of the vacuolar proton-translocating ATPase in multiple steps of the endo-lysosomal system and in the contractile vacuole system of Dictyostelium discoideum. J. Cell Sci. 109: 1479-1495 [Abstract]
Adessi, C, Chapel, A, Vincon, M, Rabilloud, T, Klein, G, Satre, M, Garin, J (1995). Identification of major proteins associated with Dictyostelium discoideum endocytic vesicles. J. Cell Sci. 108: 3331-3337 [Abstract]
Bush, J, Nolta, K, Rodriguez-Paris, J, Kaufmann, N, O'Halloran, T, Ruscetti, T, Temesvari, L, Steck, T, Cardelli, J (1994). A Rab4-like GTPase in Dictyostelium discoideum colocalizes with V-H(+)-ATPases in reticular membranes of the contractile vacuole complex and in lysosomes. J. Cell Sci. 107: 2801-2812 [Abstract]
Aubry, L, Klein, G, Martiel, J., Satre, M (1993). Kinetics of endosomal pH evolution in Dictyostelium discoideum amoebae. Study by fluorescence spectroscopy. J. Cell Sci. 105: 861-866 [Abstract]
Neuhaus, E. M., Almers, W., Soldati, T. (2002). Morphology and Dynamics of the Endocytic Pathway in Dictyostelium discoideum. Mol. Biol. Cell 13: 1390-1407 [Abstract] [Full Text]