Identification of an epithelial protein related to the desmosome and intermediate filament network

doi:10.1083/jcb.118.6.1477

This Article

	Full Text (PDF, 5417K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Ouyang, P.
	Articles by Sugrue, S. P.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Ouyang, P.
	Articles by Sugrue, S. P.


Social Bookmarking

	What's this?

ARTICLES

Identification of an epithelial protein related to the desmosome and intermediate filament network

P Ouyang and SP Sugrue
Department of Anatomy and Cellular Biology, Harvard Medical School, Boston, Massachusetts 02115.

Using a mAb, referred to as 08L, we have identified a protein, of M(r) approximately 140,000, associated with desmosomes of epithelial cells. The 08L antibody stained the intracellular side of lateral cell margins of monolayer epithelial cells but did not stain cell margins free of cell contact. Immunoelectron microscopy revealed that the 08L antigen was localized to the cytosolic surface of the desmosomal plaque near points of intermediate filament convergence with apparently little staining of the desmosomal plaque proper. Western blots revealed the 08L antigen to be a protein, of M(r) approximately 140,000, found in the Triton-X 100 insoluble pellet. High salt-containing buffers extracted the 08L antigen from the insoluble material. Examination of the assembly of 08L to the desmosome complex, in cells grown in low confluent culture or in calcium-switch assays, by double immunofluorescence with 08L and anti-desmoplakin antibody, revealed that 08L was recruited to morphologically identifiable desmosomes. 08L antigen may exist in a cytosolic pool prior to assembly to the cell surface. The solubility of 08L in low calcium and normal calcium conditions, however, was similar. 08L association to the desmosome was correlated with increased organization of the intermediate filament network. We suggest that the 08L antigen may be involved in the organization and stabilization of the desmosome-IF complexes of epithelia.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Alpatov, R., Shi, Y., Munguba, G. C., Moghimi, B., Joo, J.-H., Bungert, J., Sugrue, S. P. (2008). Corepressor CtBP and Nuclear Speckle Protein Pnn/DRS Differentially Modulate Transcription and Splicing of the E-Cadherin Gene. Mol. Cell. Biol. 28: 1584-1595 [Abstract] [Full Text]
Hsiao, V. C., Tian, R., Long, H., Der Perng, M., Brenner, M., Quinlan, R. A., Goldman, J. E. (2005). Alexander-disease mutation of GFAP causes filament disorganization and decreased solubility of GFAP. J. Cell Sci. 118: 2057-2065 [Abstract] [Full Text]
Alpatov, R., Munguba, G. C., Caton, P., Joo, J. H., Shi, Y., Shi, Y., Hunt, M. E., Sugrue, S. P. (2004). Nuclear Speckle-Associated Protein Pnn/DRS Binds to the Transcriptional Corepressor CtBP and Relieves CtBP- Mediated Repression of the E-Cadherin Gene. Mol. Cell. Biol. 24: 10223-10235 [Abstract] [Full Text]
Zimowska, G., Shi, J., Munguba, G., Jackson, M. R., Alpatov, R., Simmons, M. N., Shi, Y., Sugrue, S. P. (2003). Pinin/DRS/memA Interacts with SRp75, SRm300 and SRrp130 in Corneal Epithelial Cells. IOVS 44: 4715-4723 [Abstract] [Full Text]
Li, C., Lin, R.-I., Lai, M.-C., Ouyang, P., Tarn, W.-Y. (2003). Nuclear Pnn/DRS Protein Binds to Spliced mRNPs and Participates in mRNA Processing and Export via Interaction with RNPS1. Mol. Cell. Biol. 23: 7363-7376 [Abstract] [Full Text]
Shi, J., Sugrue, S. P. (2000). Dissection of Protein Linkage between Keratins and Pinin, a Protein with Dual Location at Desmosome-Intermediate Filament Complex and in the Nucleus. J. Biol. Chem. 275: 14910-14915 [Abstract] [Full Text]
Shi, Y., Tabesh, M., Sugrue, S. P. (2000). Role of Cell Adhesion-Associated Protein, Pinin (DRS/memA), in Corneal Epithelial Migration. IOVS 41: 1337-1345 [Abstract] [Full Text]
Wallis, S., Lloyd, S., Wise, I., Ireland, G., Fleming, T. P., Garrod, D. (2000). The alpha Isoform of Protein Kinase C Is Involved in Signaling the Response of Desmosomes to Wounding in Cultured Epithelial Cells. Mol. Biol. Cell 11: 1077-1092 [Abstract] [Full Text]
Kowalczyk, A. P., Bornslaeger, E. A., Borgwardt, J. E., Palka, H. L., Dhaliwal, A. S., Corcoran, C. M., Denning, M. F., Green, K. J. (1997). The Amino-terminal Domain of Desmoplakin Binds to Plakoglobin and Clusters Desmosomal Cadherin-Plakoglobin Complexes. JCB 139: 773-784 [Abstract] [Full Text]
Hatzfeld, M, Kristjansson, G., Plessmann, U, Weber, K (1994). Band 6 protein, a major constituent of desmosomes from stratified epithelia, is a novel member of the armadillo multigene family. J. Cell Sci. 107: 2259-2270 [Abstract]